AIM:To establish a simple and accurate HPLC method for quality evaluation and determination of 8 active constituents, i.e. Danshensu, Protocatechualdehyde,rosmarinic acid,Salvianolic acid B, Dihydrotanshinone, Cryptotanshinone, Tanshinone Ⅰand Tanshinone ⅡA in Radix Salviae Miltiorrhizae, at the same time, in order to study the correlations of the contents of the 8 constituents. METHODS: Detected at 286 nm, analytes were separated on a ODS C_ 18 column(150 mm?4.6 mm, 10 ?m) eluted gradually with methanol and 1% tetrafuran in 1% formic acid water solution(3∶97)on the increasement of methanol from 3% to 70% in the whole analysis. After determination, SPSS 13.0 software was used to calculate the correlation between the 8 constituents. RESULTS: Under the above conditions, HPLC method was constructed to quantify 8 constituents,and their correlation coefficients were calculated. CONCLUSION: The result shows that the contents of the 8 constituents in Salvia miltiorrhiza Bge. vary greatly from different origins; and there are significant correlations between the contents of hydrophilic constituents such as Salvianolic acid B,Danshensu,rosmarinic acid, so do hydrophobic constituents like Cryptotanshinone,Tanshinone Ⅰ,Tanshinone ⅡA,but there is no correlation between the hydrophilic constituents and hydrophobic constituents.

Objective To explore the influencing factors of disability degree in elderly hospital-ized patients with disability and the correlations of disability with malnutrition and sarcopenia.Meth-ods A total of 234 elderly hospitalized patients with disability were selected as study subjects.The Activities of Daily Living(ADL)scale,Mini-nutritional Assessment Short Form(MNA-SF),and Sarcopenia Five-item Criteria with calf circumference(SARC-CalF)were used to investigate the sta-tus of disability,nutrition,and sarcopenia in patients.The clinical characteristics of different types of patients were compared,and correlations of disability with malnutrition and sarcopenia were analyzed using Pearson correlation analysis.Multiple linear regression analysis was used to explore the influen-cing factors of the degree of disability in patients.Results Of the 234 patients,46.58％(109/234)had mild disability,45.30％(106/234)had moderate disability,and 8.12％(19/234)had severe disability.The results of Pearson correlation analysis showed that the degree of disability(ADL score)in elderly hospitalized patients with disability was significantly correlated with the degree of malnutrition(MNA-SF score)and sarcopenia(SARC-CalF score)(r=0.177,-0.193,P=0.007,0.003).Multiple linear regression analysis showed that malnutrition and sarcopenia were in-fluencing factors of the degree of disability in elderly hospitalized patients with disability(P＜0.05).Conclusion There are correlations of disability with malnutrition and sarcopenia in elderly hospital-ized patients with disability.The risk of exacerbation of disability is significantly increased in patients with malnutrition and sarcopenia.Therefore,clinicians need to identify high-risk patients as early as possible and provide targeted intervention measures.

Objective To explore the influencing factors of disability degree in elderly hospital-ized patients with disability and the correlations of disability with malnutrition and sarcopenia.Meth-ods A total of 234 elderly hospitalized patients with disability were selected as study subjects.The Activities of Daily Living(ADL)scale,Mini-nutritional Assessment Short Form(MNA-SF),and Sarcopenia Five-item Criteria with calf circumference(SARC-CalF)were used to investigate the sta-tus of disability,nutrition,and sarcopenia in patients.The clinical characteristics of different types of patients were compared,and correlations of disability with malnutrition and sarcopenia were analyzed using Pearson correlation analysis.Multiple linear regression analysis was used to explore the influen-cing factors of the degree of disability in patients.Results Of the 234 patients,46.58％(109/234)had mild disability,45.30％(106/234)had moderate disability,and 8.12％(19/234)had severe disability.The results of Pearson correlation analysis showed that the degree of disability(ADL score)in elderly hospitalized patients with disability was significantly correlated with the degree of malnutrition(MNA-SF score)and sarcopenia(SARC-CalF score)(r=0.177,-0.193,P=0.007,0.003).Multiple linear regression analysis showed that malnutrition and sarcopenia were in-fluencing factors of the degree of disability in elderly hospitalized patients with disability(P＜0.05).Conclusion There are correlations of disability with malnutrition and sarcopenia in elderly hospital-ized patients with disability.The risk of exacerbation of disability is significantly increased in patients with malnutrition and sarcopenia.Therefore,clinicians need to identify high-risk patients as early as possible and provide targeted intervention measures.

Objective To study the different drying technology.on Wudang Lonicerae Flos (Ⅰ) in chlorogenic acid content.Methods Using HPLC method to research the difference in the contents of chlorogenic acid of Wudang Lonicerae Flos (Ⅰ) employing oven-drying method,air-dried method and steamed drying technologies.DIONEX C18 (4.6 mm×250 mm,5 μm) was used.The mobile phases consisted of acetonitrile-0.4％ phosphoric acid aqueous solution (17 ∶ 83).The detection wavelengths of chlorogenic acid was 327nm.The flow rate was 0.4 ml/min.The column temperature was 30 ℃.Results The Separation of Chlorogenic acid content was satisfactory.The concentration and peak area showed a good linearity relationship with the range of 0.285-2.850 μg.Precision,repeatability and stability met the requirements.The average recovery was 100.26％ with RSD 1.95％.Chlorogenic acid content in the steamed drying Wudang Honeysuckle (Ⅰ) was higher.Conclusions The method of steam drying is better than that of oven-drying method and drying.