Objective To explore the effect of comprehensive interventions on protective behaviors of sanitary workers against occupational exposure in hospitals. Methods Comprehensive interventions were done in 50 sanitary workers in our hospital during September 2013 to September 2014, with the intervention contents including establishment of sanitary workers management system, education training, on-the-spot inspection, signature of duty informs, planning of occupational exposure prevention flow chart. The intervention lasted 12 months. Then the score on occupational exposure prevention knowledge and behaviors, the qualification rate of all indexes and all occupational harms in pre-intervention and post-intervention were compared. Result The score on occupational exposure preventive knowledge after intervention was significantly higher than that before intervention , the qualification rate after intervention was higher than that before intervention and the incurrence of occupational harms was significantly lower than that before intervention (P < 0.01). Conclusion Multiple measures including education, supervision, inspection, duty informing and flow chats can strengthen the awareness of preventing occupational exposure and lower the incidence of occupational harms among the sanitary workers.

In order to investigate the type of infiltrating T cells in allergic contact dermatitis (ACD) reaction, by the methods of RT-PCR and immunohistochemistry, we tried to detect IL-2mRNA, IL-2, IFN-?, IL-4 in the lesions of patients with ACD. The results showed that Th1 cytokines were expressed aberrantly in ACD patients and Th1-like clone which secrets IL-2, IFN-? appeared to be predominant in ACD reaction.

Objective To discuss the effect of Human Immunoglobulin for Intravenous Injection in the treatment of neonatal pneumonia treatment and intervention. Methods 100 cases of newborns in our hospital from July 2015 to 2016 to receive the treatment of infectious pneumonia patients, and were randomly divided into control group and observation group, 50 cases in each group. The control group used routine treatment, the observation group on the basis of intravenous immunoglobulin and take corresponding nursing measures. The treatment were compared between the two groups of the total efficiency of disappearance of hospitalization time and pulmonary rales and difficulty breathing time. Results In the control group than the treatment in the observation group the total efficiency significantly comparison between the two groups of children have statistical significance(P<0.05); disappeared in the observation group hospitalization time and pulmonary rales and dyspnea was significantly better on time According to the group, there were significant differences between the two groups have statistical significance(P<0.05). Conclusion The use of Human Immunoglobulin for Intravenous Injection on the treatment of neonatal pneumonia, can effectively promote the treatment efficiency, and is helpful to the patient's recovery, worthy of promotion in clinical use.

Objective: To evaluate the application value of fluorine-18-fludeoxyglucose (18F-FDG) PET/CT maximal standard uptake (SUVmax) in the diagnosis of lymphoma. Methods: A total of 103 patients with lymphoma which confirmed by pathological diagnosis in Gansu Provincial Hospital from January 2011 to December 2016 were collected. All patients underwent a whole body simultaneous18F-FDG PET/CT and had complete clinical data. The possible relationships of SUVmax with the different pathological subtypes, clinical stages and risk grades of lymphoma were analyzed. The correlations of SUVmax value with Ki-67 index, lactate dehydrogenase (LDH) and erythrocyte sedimentation rate (ESR) were analyzed. Results: There was no significant difference in SUVmax between Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL) (P > 0.05). The SUVmax in patients with strongly invasive diffuse large B-cell lymphoma (DLBCL), T-lymphoblastic lymphoma (T-LBL), anaplastic large cell lymphoma (ALCL) and natural killer (NK)/T cell lymphoma was higher than that in patients with relatively weakly invasive mantle cell lymphoma (MCL), peripheral T-cell lymphoma (PTCL), small lymphocytic lymphoma (SLL), follicular lymphoma (FL) (grade 1-2) and marginal zone lymphoma (MZL) (P < 0.05). The SUVmax in the patients with DLBCL was higher than that in the patients with MCL, PTCL, SLL, FL and MZL, respectively (all P < 0.05). The SUVmax in the patients with ALCL was higher than that in the patients with FL (P < 0.05). The differences of SUVmax among other subtypes were not statistically significant (P > 0.05). The SUVmax had no significant difference between stage -Ⅱ and III- in the patients with lymphoma (P > 0.05). The SUVmax in the patients with high-risk NHL was higher than that in the patients with intermediate- and low-risk NHL (both P < 0.01). For 103 patients with lymphoma, the SUVmax obviously had a positive correlation with Ki-67 index (r = 0.67, P < 0.001). There was no significant difference in SUVmax between the ESR increasing group and the normal group as well as between the LDH increasing group and the normal group (both P > 0.05). Conclusion:18F-FDG PET/CT SUVmax can be used as a reference indicator for evaluating the invasive degree of lymphoma, predicting Ki-67 index, and judging the risk classification of NHL.

Objective To investigate the serum concentration of adiponectin(apM1)in patients of Han population with type 2 diabetes(T2DM), impaired glucose tolerance(IGT)and normal glucose tolerance(NGT)in Liaoning Province,and explore its correlation with T2DM process. Meth-ods Unrelated 278 subjects in Liaoning Province were enrolled for the study,including 97 newly diagnosed T2DM subjects,88 IGT subjects,and 93 cases with NGT. Serum apM1,fasting and post-glucose load glucose and insulin,blood lipids,blood pressure,calculate HOMA-IR and HOMA-B,height,weight,waist,hips were measured;body mass index(BMI)and waist hip ratio(WHR)were calculated. Three groups were divided into obese overweight and non-obese overweight subgroups with BMI≥25 kg/m2 as the boundary point. Then central and peripheral obesity was distin-guished using WHR(male≥0.90,female≥0.85 as the boundary point). Results Reduced apM1 levels was first observed in obese NGT stage. Accompanied by elevated levels of insulin resistance,apM1 levels showed a gradual decline trend paralleled with the NGT-IGT-T2DM model. Se-rum apM1 in T2DM was significantly lower than that in NGT(P<0.001). Negative correlations were found between apM1 and WHR,fasting plas-ma glucose,HOMA-IR and triglyceride. Stepwise multiple regression analysis indicated that,HOMA-IR and triglyceride were independent factors for serum apM1 levels. Conclusion Reduced apM1 levels were first appeared in obese NGT stage;apM1 is closely related to insulin resistance and T2DM process. Low level of apM1 can be used as a predictor of early intervention of IGT outcome and T2DM process.

OBJECTIVE:To provide reference for medication and chemotherapy in patients with colorectal cancer. METH-ODS:Through retrospective study,case histories and doctor’s advice of the colorectal cancer patients receiving chemotherapy in our hospital during 2011-2014 were consulted to analyze the chemotherapy,medication and irrational drug use. RESULTS:A total of 593 cases of colorectal cancer patients with chemotherapy frequency of 1 940 times were collected to analyze the choice of che-motherapy regimen mainly from the following aspects:indications of chemotherapy,choice of chemotherapy regimen,chemothera-py process and chemotherapy period. There was 409 cases of irrational drug use according to the analysis of drug dosage,selection of solvents and drug concentration,the unqualified rate was 21.08% . CONCLUSIONS:The medication and chemotherapy in pa-tients with colorectal cancer in our hospital are basically rational,but there are still certain problems and shortcomings. In future clinical applications,the medication and chemotherapy need to be improved.

Objective To establish an HPLC method for the content determination of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from different habitats.Methods The chromatographic separation was performed on an Agilent C18 column (4.6 mm×150 mm, 5μm) with mobile phase of acetonitrile-water solution with gradient elution at the flow rate of 0.8 mL/min; the detection wavelength was 208 nm; the column temperature was 30℃; the injection volume was 20μL.Results Protodioscin showed a good linear relationship among the range of 1.73–8.64 μg (r=0.999 6), with the average recovery of 101.98% (RSD=1.53%); Diosgenin showed a good linear relationship among the range of 1.03–8.20μg (r=0.999 1), with the average recovery of 101.60% (RSD=2.41%). The contents of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from 10 different habitats were in the range of 0.89%–2.24% and 0.75%–3.22%, respectively.Conclusion The method is simple, accurate and with repeatability, which can be used as quality control method of Dioscoreae Hypoglaucae Rhizoma.

OBJECTIVE:To study the protective effects of Li medicine Chushi qufeng decoction on arthritis model rats. METH-ODS:60 rats were randomly divided into normal group,model group,positive group and Chushi qufeng decoction high-dose,me-dium-dose and low-dose groups [45.9,22.95,11.48 g(crude drug)/kg]. Except for normal group,those groups were given chicken type Ⅱ collagen to induce arthritis model. After modeling,normal group and model group were given normal saline intragastrical-ly,once a day,for consecutive 12 d;Chushi qufeng decoction groups were given relevant medicine intragastrically;positive group was given leflunomide 4.5 mg/kg on 1st-3rd day and 1.8 mg/kg on 4th-12th day. The degree of joint lesion in rats were scored. The degree of joint swelling was determined as well as the serum levels of TNF-α,IL-1β and type Ⅱ collagen antibody. RESULTS:Compared with normal group,arthritis index,degree of joint swelling,the serum levels of TNF-α,IL-1βand typeⅡcollagen anti-body increased significantly in model group (P<0.01). Compared with model group,pathological score of positive group and Chushi qufeng decoction high-dose group decreased significantly,and serum levels of TNF-α,IL-1β and type Ⅱ collagen antibody decreased significantly in treatment groups(P<0.05 or P<0.01). CONCLUSIONS:Li medicine Chushi qufeng decoction has cer-tain protective effect on arthritis model rats induced by chicken typeⅡcollagen.

Objective : To examine the effect of asbestos exposure on oxidative stress, so as to provide insights into the elucidation of pathogenesis and management of asbestos-related diseases. Methods : Totally 245 subjects were recruited from an asbestos manufacturing area in Zhejiang Province, and their gender, age and history of asbestos exposure were collected through a questionnaire survey. The serum levels of 8-hydroxy-2'deoxyguanosine ( 8-OHdG ), glutathione ( GSH ), malondialdehyde ( MDA ), superoxide dismutase ( SOD ) and total antioxidative capacity ( TAOC ) were measured using an enzyme-linked immunosorbent assay ( ELISA ), and the levels of catalase ( CAT ), peroxiredoxin 2 ( PRX2 ), SOD1, SOD2 and thioredoxin-1 ( TRX1 ) were detected in peripheral white blood cells ( WBCs ) using a liquid-chip assay. Multivariable linear regression analysis was performed to identify the association between asbestos exposure and oxidative stress parameters. Results : There were 50 subjects without a history of asbestos exposure (unexposed group), 102 subjects with asbestos exposure for less than 10 years ( AE<10-year group ) and 93 subjects with asbestos exposure for 10 years and more ( AE≥10-year group ). No significant differences were found among the three groups in terms of age, gender, proportion of smokers or proportion of alcohol consumers ( P>0.05 ). Significantly higher 8-OHdG and MDA in serum, and higher PRX2 in peripheral WBCs were detected in the AE≥10-year group than in the unexposed group ( P<0.05 ); lower GSH and TAOC in serum, and lower CAT in peripheral WBCs were detected in the AE≥10-year group than in the unexposed group ( P<0.05 ); higher 8-OHdG and MDA in serum, and higher PRX2 in peripheral WBCs were detected in the AE≥10-year group than in the AE<10-year group ( P<0.05 ). Multivariable linear regression analysis showed that asbestos exposure significantly correlated with 8-OHdG, MDA and TAOC in serum, and CAT and PRX2 in peripheral WBCs ( P<0.05 ). Conclusion Asbestos exposure may induce the oxidative stress damage, suggesting that oxidative stress may be involved in asbestos-related diseases.

Objective To study the significance of combined detection of procalcitonin ( PCT ) , C-reactive protein( CRP) and lipopolysaccharide( LPS) for early diagnosis of bacterial infection in newborns. Methods Clinical data of ninety-eight newborns from neonatal ward of our hospital were retrospectively studied. Fifty cases with bacterial infectious diseases were selected as infection group,in the same period,48 cases with non-bacterial infectious diseases were selected as control group. In the 24 hours after admission before use of antibiotics,all of cases were picked blood used for testing CRP,PCT,LPS and blood culture, and the results were contrasted and analyzed. Results The levels of serum PCT,CRP and LPS in infection group were respectively significantly higher than those in control group,and the differences were statistically significant(P<0. 05,respectively). In gram-positive bacterium group,the positive rate of combined detection of serum PCT and CRP was obviously higher than that of single detection of PCT or CRP ( 91. 3% vs. 60. 9%,P<0. 05;91. 3% vs. 56. 5%,P<0. 05 respectively) . In gram-negative bacteria group,positive rate of combined detection of serum PCT and LPS was obviously higher than that of single detection of PCT or LPS respectively(88. 9% vs. 59. 3%,P<0. 05;88. 9% vs. 66. 7%,P<0. 05 respectively). Conclusion Joint detection can improve the diagnostic efficiency, and reduce missed diagnosis. And we can identify disease which predominantly infected by positive bacteria or negative bacteria through joint detection,which can contribute to the choice of clinical antibiotic drugs.