Objective To study the effect of PinXl on radiosensitivity and ROS level of esophageal cancer cells.Methods Esophageal cancer cells were divided into control group (no cell transfection),radiation group (8 Gy of X-rays),PinX1 group (pDsRed1-PinX1 transfect to overexpression,radiation+PinX1 group (8 Gy X-rays after pDsRed1-PinX1 transfection).Cell proliferation was measured by MTT assay,cell apoptosis was measured by flow cytometry,the activation levels of Caspase-3 and Caspase-9 in cells were measured by Western blot,the cellular ROS level was determined using DCFH-DA probe,and the cell radiosensitivity was evaluated by the colony formation assay.The subcutaneous xenograft tumor model of EC9706 cells was established with nude mice,and each group received local irradiation every 4 days 8 Gy five fractions γ-rays.The nude mice bearing PinX1-transfected cells were implanted with PinX1 plasmid into tumor before radiotherapy to investigate the relationship between PinX1 overexpression and radiosensitivity in xenotransplantation mice models.Results Compared with control group cells,the survival rate of EC9706 cells of radiation group or PinX1 group decreased significantly from 100％ to (67.92±4.71) ％ and (83.14 ±4.01) ％ (t =9.433,4.957,P＜0.05),the apoptosis rate increased significantly from (6.47± 1.46) ％ to (44.38±4.16) ％ and (25.42 ±2.78) ％ (t =12.882,6.439,P ＜ 0.05),Caspase-3 activation level increased (t =6.655,2.531,P ＜0.05),and the activation level of Caspase-9 and cellular ROS was also significantly increased.Compared with radiation group cells,the cell viability of radiation+PinX1 group decreased from (67.92 ±4.71) ％ to (52.73 ±5.58) ％ (t =8.942,P＜0.05),the apoptosis rate increased from (44.38±4.16) ％ to (55.29±4.98)％ (t=3.707,P＜0.05),the activation levels of Caspase-3,-9,and the level of ROS in cells was increased (t =15.435,17.990,4.526,P＜0.05).The radiosensitivity of EC9706 cells was increased after PinX1 transfection with a sensitization ratio of 1.408.The volume of xenograft tumor with PinX1 overexpression cells in nude mice was significantly smaller than control.Conclusions Overexpression of PinX1 could inhibit the proliferation,induce apoptosis and increase the radiosensitivity of esophageal cancer cells.

Objective:To explore the application of photoplethysmography (iPPG) for contactless vital signs monitoring in the intensive care unit (ICU).Methods:Ten tracheostomy patients in intensive care had their heart rate, oxygen saturation, and diastolic and systolic pressures monitored using iPPG technology and a 24-hour bedside monitor. The readings included periods at rest, during turning, during suctioning, and when undergoing vigorous physical therapy and occupational therapy. The monitoring lasted 3 consecutive days. The data collected by the two methods were compared to analyze the accuracy of the contactless vital signs monitoring system.Results:The oxygen saturation readings of the two systems showed no significant differences. The heart rates, diastolic pressures, and systolic pressures did, however, differ significantly.Conclusions:In the situations tested, contactless monitoring of oxygen saturation is effective, but there is still significant room for improvement in the three indicators of heart rate, systolic pressure, and diastolic pressure.