To investigate the effects of medicated serum prepared with Chinese herbal medicine Zhizhen Recipe (ZZR) on activity of nuclear factor-κB (NF-κB) and expression and function of P-glycoprotein (P-gp) in human colorectal cancer multidrug-resistant cell line HCT-8/VCR.

Objective:To explore the relationship between ten‐eleven translocation 2 (TET2) Mutations and Expression of 5hmC in breast cancer .Methods :TET2 mutations in breast cancer tissues from 100 patients were detected by Sanger method . The expression of 5hmC in breast cancer tissues was detected by immunohistochemistry . The relationship between TET2 mutations and expression of 5hmC was analyzed .Results :A total of 13 kinds of TET2 mutations were identified in 100 patients .Among them ,3 new types of mutations were identified in 4 patients . They were Ala341Val , Leu1622Phe and Pro1578Tyr .Pro1578Tyr was identified in two patients .And immunohistochemical analysis showed that there was a sharp reduction of 5hmC in breast cancer tissues with TET2 mutations than in breast cancer tissues without TET2 mutations . Conclusions :T ET2 mutations can lead to decrease of 5hmC level in breast cancer .