Anesthesia, General ; adverse effects ; Child, Preschool ; Humans ; Male ; Malignant Hyperthermia ; diagnosis ; etiology

Modern management is in essence management of people and the idea and strategy of humanistic management infiltrate into various management activities of a hospital. Our hospital has attempted to enhance the ideological quality and vocational level of the nursing staff by the theory of humanistic management. As a result of such measures as stimulating the nursing staff's thirst for knowledge, enlarging the scope of rewards, increasing the work pressure of young nurses in a proper way, providing room for the free development of head nurses, implementing dynamic management, and adopting democratic management with a high degree of openness, the ideological quality of the nurses has been greatly enhanced. At the same time, measures have been taken to ensure from 3 links that their vocational level keeps improving as well.

Objective To study the possibility of fetal cardiac rhabdomyoma diagnosed by ultrasonography,and the relationship between the fetal cardiac rhabdomyoma and family tuberous sclerosis. Methods High resolution ultrasonic equipment with fetal cardiac software was used. The main cross section of four chamber view was studied mainly. The location, the number of the tumor and the outflow tract of left and right ventricular cordis were scanned carefully, the maternal abdominal scan was performed as well. Results Five cases of fetal cardiac rhabdomyoma were found by routine prenatal ultrasonography, and were confirmed by autopsy. Among them, one case were associated with maternal tuberous sclerosis. Conclusions Fetal cardiac rhabdomyoma associated with tuberous sclerosis. Fetus with cardiac rhabdomyoma frequently die of cardiac failure; since the prognosis of the tuberous sclerosis with neural sclerosis was very poor, when the fetal cardiac rhabdomyoma was detected prenatally, the possibility of tuberous sclerosis should be considered, also relative examination must be performed to the mother to decrease the perinatal mortality.

Adult ; Benzene ; poisoning ; Cord Blood Stem Cell Transplantation ; Female ; Humans ; Infant, Newborn ; Leukemia ; etiology ; surgery ; Male ; Treatment Outcome ; Young Adult

Objective To investigate the anticancer effects and molecular mechanism of betulinic acid (BA)on Raji cells in vitro.Methods The effects of BA on the growth of Raji cells were studied by MTT assay.Apoptosis was assessed by Annexin-V/PI double-labeled cytometry.The influence on cell cycle was studied by flow cytometer.The cyclin D3 mRNA expression was checked by Western blotting and RT-PCR techniques.Results BA showed obvious inhibition on proliferation,as well as induction potency of apoptosis on Raji cells in vitro in a time-and dose-dependent manner by Annexin-V/PI double-labeled method.With the IC_(50)value for 24 h being(39.44?0.65)?g/mL,Raji cells treated with BA showed ac- cumulation in G_0/G_1 phase and reduction in the percentage of cells in S phase.The cyclin D3 mRNA ex- pression and protein were sharply decreased in Raji cells treated with BA.Conclusion BA could inhibit the proliferation of Raji cells by regulating the cell cycle that arrests cells at G_0/G_1 phase and induces apop- tosis of Raji cells.The antitumor effects of BA may be related to down-regulation of the expression of cy- clin D3.

ObjectiveTo study the prevalence rate of post-stroke depression (PSD) of the elderly in the community and analyze risk factors of them. MethodsThe elders of the age of 60 or over were sampled as subjects from a community. Zung (SDS) was used as screening instruments and Hamilton rating scale for depression (HRSD-24) as evaluating scales. The prevalence rate of PSD was surveyed. A structured questionnaire was used and physical examination was carried out. Risk factors measured included baseline information, daily habits, and history of main diseases (hypertension, coronary heart disease, diabetes mellitus, myocardial infarction, and so on). The relationship between risk factors and PSD was analyzed.ResultsThe prevalence of PSD was 9.5%. The risk factors for PSD were life quality and age. ConclusionPost-stroke depression disturbs elder's health and rehabilitation. The prevalence rate and harm of PSD are higher than depression without stroke.

Objective Mikulicz's disease (MD) was considered to be a subtype of Sj(o)gren's syndrome (SS) due to the clinical and histological similarities between them.Evidence had shown that there were differences between MD and typical SS.The purpose of this study was to investigate the correlation between MD and SS,by means of analyzing the expression of IgG4 in salivary glands and the clinical characteristics of patients who were previously considered as SS.Methods The paraffin sections of salivary glands from SS patients were stained with monoclonal antibodies to IgG4 and CD38.Patients were divided into two groups based on the pathological results.Analysis of the symptoms,the signs and the laboratory results were carried out in these patients.The difference in laboratory parameters and histopathological gradings in the two groups was analyzed.Normal and abnormal distributed data comparison was conducted using random independent samples t-test and rank sum test respectively.Two-sample rates were compared with Chi-square test.Results Based on immunohistochemistry of IgG4 distribution,the 58 patients with SS were divided into two groups:IgG4 related (9 cases) and non-IgG4 related (49 cases).Histopat-hologically,IgG4 related cases showed IgG4+ plasma cells/IgG+ plasma cells infiltration and there were more IgG4 related monoclonal antibody expressed when compared to IgG4 unrelated cases.In addition,there were also significant differences in clinical features between the two groups.IgG4 related disease was associated with male gender,higher level of plateletconnt,lymphocytes [(2.4±0.8)×109/L vs (1.4±0.7) ×109/L] count and CRP [(52±60) mg/L vs (15±17) mg/L] levels and lower titer of IgM [(1.2±0.7) g/L vs (1.8±0.8) g/L],antinuclear antibody (56％ vs 87％) and anti-SSB antibodies (13％ vs 54％) (P＜0.05),when compared with IgG4-unrelated cases.There was no significant difference in other indicators (P＞0.05).Conclusion The present study has demonstrated that some of the MD patients misdiagnosed as SS.Some of the laboratory tests such as the level of platelet and lymphocyte count,serum level of CRP,IgM,antinuclear antibody,anti-SSB antibodies,the serum levels of IgG4 and the histopathological presentations in the salivary gland are different between these two disorders.Because of good response to steroid in MD,so laboratory tests and pathological examinations for IgG4 can help to avoid misdiagnosis.

AIM: To study the effects of Ginkgo biloba extract (GbE) on cerebral ischemia-reperfusion injury. METHODS: Cerebral ischemia-reperfusion injury was produced by 10 min or 20 min occlusion of bilateral carotid arteries followed by 5 d or 1 d reperfusion in gerbils. Ninety-five gerbils were divided into 4 groups: sham-operation, ischemia-reperfusion, GbE 50 mg*kg-1 and GbE 100 mg*kg-1 groups. Drugs were given intragastrically 2 d prior to ischemia and during reperfusion. The effects of GbE on the contents of calcium, sodium, water in cortex, and lipid peroxide(LPO) in brain hemispheres, as well as the density of neuron in hippocampal CA1 sector were observed. RESULTS: GbE could reduce the increase of calcium, sodium, water content in a manner of dose-depedance. The dosage of GbE 100 mg*kg-1 could decrease the content of LPO and the mortality, increase the density of neuron in hippocampal CA1 sector. CONCLUSION: GbE has protective effects on cerebral ischemia reperfusion injury.

The paper introduces and summarizes the teaching purpose and content project,implementation and effect of the optional course in Excel and medical data analysis.

Objective:To explore the incidence,mortality,trends and time distribution of food poisoning in Hunan Province.Methods:The data on food poisoning was derived from the Information Office of Hunan Provincial Health Department.Using the trend-test and circular distribution methods,we have described the current situation of food poisoning and tested the central tendency of the peak time points and the peak time zone of food poisoning in Hunan from 2000 to 2009.Results:On average,the incidence of food poisoning in Hunan from 2000 to 2009 was 0.072 per 100000 population.And the average number of people affected in these incidents was 1.937 per 100000 population.There were no apparent trends in either the number of incidents or people affected between 2000 and 2009 (u=-0.98,P＞0.05; u=-1.34,P＞0.05,respectively).The average mortality was 0.015 per 100000 population.The trend-test indicated that the average annual mortality decreased significantly from 2000 to 2009 (u=-1.72,P＜0.05).Meanwhile the average annual fatality rate was 0.77％.The trend-test revealed statistically significant differences for the average annual fatality rate (u=-1.88,P＜0.05).The circular distribution analysis showed that there was a central tendency of the distribution of food poisoning cases,with the average peak time atAugust 28th and the average peak time zone from June 7th to November 18th for food poisoning from 2000 to 2008.Conclusion:From 2000 to 2009,there is a significant tendency in the average annual mortality and fatality rate of food poisoning in Hunan.Summer and fall are the high seasons for food poisoning.We should pay attention to the peak time zone,especially the peak time point of food poisoning for food safety monitoring,and strengthen the prevention and control on food poisoning.