1.Epidemiological study of small airway disease in Railway Area in Jinan.
Ling-min KOU ; Yan-xia GAO ; Pei-jun XIA ; Ke-hui ZHAO ; Jun WANG
Chinese Journal of Epidemiology 2004;25(11):1010-1010
Adult
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Aged
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Asthma
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epidemiology
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etiology
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Bronchiolitis Obliterans
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epidemiology
;
etiology
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China
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epidemiology
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Female
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Humans
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Male
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Middle Aged
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Prevalence
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Smoking
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adverse effects
2.Surveillance on HIV-1 incidence among men who have sex with men in Chongqing, China,2006-2008
Mei HAN ; Lian-Gui FENG ; Yan JIANG ; Sheng SHEN ; Hua LING ; Xian-Bin DING ; Lin OUYANG ; Jin-Kou ZHAO ; Yao XIAO ; Min ZHANG
Chinese Journal of Epidemiology 2009;30(9):878-881
n increasing trend.
3.Heteroclitins R-S: new dibenzocylooctadiene lignans from Kadsura heteroclita.
Min CHEN ; You-Ping LUO ; Yan-Lin ZOU ; Ling-Hu LANG ; Dao-Feng CHEN
Chinese Journal of Natural Medicines (English Ed.) 2014;12(9):689-692
AIM:
To study the dibenzocylooctadiene lignans from the stems of Kadsura heteroclita.
METHOD:
Chromatographic separations of silica gel and semi-preparative HPLC were used. All of the structures were elucidated on the basis of spectroscopic analysis, including 2D-NMR and HR-MS techniques.
RESULTS:
Four dibenzocylooctadiene lignans were isolated from K. heteroclita. Their structures were identified as heteroclitin R (1), heteroclitin S (2), gonisin O (3), and schisanlignone A (4).
CONCLUSION
Heteroclitin R (1) and heteroclitin S (2) are new natural lignans.
Kadsura
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chemistry
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Lignans
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chemistry
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isolation & purification
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Molecular Structure
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Plant Extracts
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chemistry
4.Expression Level and Target Gene Prediction of miR-181b in Patients with Chronic Lymphocytic Leukemia.
Zhen KOU ; Hong LIU ; Yi-Chun WANG ; Qin HUANG ; Zeng-Sheng WANG ; Zai-Li Nu Er GU ; Tao LANG ; Yu-Ling NIE ; Li AN ; Zi-Gu Li A ; He-Ta Bai Er MU ; Xiao-Yan ZHANG ; Ling FU ; He-Mai Jiang AI ; Min MAO ; Xiao-Min WANG ; Yan LI
Journal of Experimental Hematology 2020;28(3):808-814
OBJECTIVE:
To investigate the expression level of miR-181b in CD19+ B lymphocytes of patients with chronic lymphocytic leukemia (CLL), to analyze the relationship between its expression and the prognosis of CLL patients, and to predict the potential target gene of miR-181b in CLL by using bioinformatics.
METHODS:
Eight-four patients with CLL treated in People's Hospital of Xinjiang Uygur Autonomous Region from June 2013 to June 2018 were selected. and 20 healthy people were selected as control group. RNA was extracted from CD19+B lymphocytes of peripheral blood by magnetic bead sorting, the expression level of miR-181b was detected, and it's expression differences in different IPI groups were analyzed. The correlation between the expression level of miR-181b and PFS of CLL patients also was analyzed. miR-181b target genes were predicted by online database and literatures, and gene annotation analysis and relevant signal pathway analysis were performed for candidate target genes.
RESULTS:
The expression level of miR-181b in CLL patients was significantly lower than that in control group (P<0.01); The expression level of miR-181b in the low-risk group was higher than that in high-risk group and extremely high-risk group (P<0.05), but there was no statistical difference between low-risk group and medium-risk group (P=1.00). The expression level of miR-181b in medium-risk group was higher than that in high-risk group and extremely high-risk group (P<0.05), but there was no difference between high-risk group and extremely high-risk group (P=1.00). ROC curve results showed that the area under the curve (AUC) was 0.792 (P<0.01).When the expression level of miR-181b was at the threshold value of 0.279, it showed a better sensitivity (62.9%) and specificity (91.8%). Survival analysis results suggested that compared with the high expression group, the miR-181b low expression group had poor PFS (log rank: P=0.047). Prediction of miR-181b by using the starBase, targetscan and picTar database and its combination with literature reports indicated that CARD11, ZFP36L1, RUNX1, NR4A3, ATP1B1, PUM1 and PLAG1 related with blood diseases, and up-regulated CARD11 and ZFP36L1 participated in lymphoid tumor formation by promoting cell proliferation and inhibiting cell aging.
CONCLUSION
The expression level of miR-181b in CLL group are significantly lower than that in the controls group, and the low expression of miR-181b relates with poor prognosis of CLL patients. Through bioinformatics prediction and combined with literature reports, it is speculated that CARD11 and ZFP36L1 as target genes of miR-181b may be participated in the occurrence and development of CLL. Further experiments are needed to verify this result.
Apoptosis Regulatory Proteins
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Cell Proliferation
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Humans
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Leukemia, Lymphocytic, Chronic, B-Cell
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genetics
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MicroRNAs
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Prognosis