OBJECTIVE:To prepare icaritin-poloxamer 188 solid dispersions in order to increase the dissolution rate of icaritin. METHODS:With poloxamer 188 as the carrier,melting method was used to prepare solid dispersions. By comparing the in vitro dissolution rates,the effects of the content of poloxamer 188(the ratios of icaritin to poloxamer 188 were 5∶1,3∶1,2∶1,1∶1,1∶3,1∶5,1∶7,1∶9,1∶11,1∶13,1∶15,1∶17,1∶19,1∶27 and 1∶31),melting temperature(60,70 and 80 ℃)and cooling tem-perature(-20,0 and 20 ℃)on the dissolution rate of icaritin in the solid dispersions were investigated,and the in vitro dissolu-tion rates of icaritin in its active pharmaceutical ingredient,physical mixture and solid dispersions were compared to confirm the for-mation of the solid dispersions. RESULTS:The dissolution rate of icaritin in the prepared solid dispersions increased to some extent as the proportion of the carrier increased. When the ratio of icaritin to the carrier was 1∶17-1∶27,the dissolution rate of icaritin at 120 min was above 90%. Where melting temperature and cooling temperature were respectively determined as 60 ℃ and 0 ℃ after comprehensive comparison,the dissolution rate of icaritin in the solid dispersions was 1.5 times as much as that in the physical mix-ture at 30 min. CONCLUSIONS:The prepared solid dispersion has a significantly higher dissolution rate of icaritin.

Objective To observe the effect and toxicity of oxaliplatin combined with calcium folinate and tegafur in the treatment of rectal cancer patients,in order to provide a safe and effective clinical program for rectal cancer treatment.Methods 117 rectal cancer patients conforming to the selection criteria were randomly divided into treatment group 59 cases and control group 58 cases by using random number method,treatment group were received chemotherapy of Oxaliplatin,calcium folinate and tegafur;control group were received FOLFOX4 program.After imple-mentation of two treatment plans,solid tumor curative effect evaluation standard from WHO was referenced to evaluate the curative effect.And toxicity classification was reached according to the WHO cancer drug toxicity assessment standard.Results Total effective rate of treatment group was 27.1%,total effective rate of control group was 29.3%,the comparative difference of two rates was not statistically significant (χ2 =0.069,P >0.05).The main symptoms of adverse reactions of two treatment plans were decreased of leukopenia,hemoglobin and thrombocytopenia of the blood system;nausea,vomiting,weak and abnormal liver function of the digestive system;,and limb numbness or pain of the nervous system.Adverse reactions difference in two groups was not significant (χ2 =0.106,0.158, 0.000,0.563,0.001,0.284,0.068,0.000,all P >0.05).After treatment,the median surial time was 15.5 months in treatment group and 16.5 months in control group,the difference of the median surial time in two groups was not statistically significant (P =0.781,P >0.05).Conclusion Combination of Oxaliplatin,calcium fluoride and tegafur is another safe and effective plan after the FOLFOX4 program in the treatment of rectal cancer drug application.

Objective To explore the therapeutic efficacy and safety of cyclophosphamide combined with MP for patients with multiple myeloma (MM),as well as its influence on serum CRP,LDH,albumin (Alb)and β2 -microglobulin (β2 -MG).Methods 6 1 patients diagnosed MM in our hospital were divided into two groups random-ly:32 cases in the treatment group were given the regimen of cyclophosphamide combined with MP,and 29 cases in the controlled group were given the regimen of MP.After four periods of treatment,the curative effects and side effects of two groups were evaluated,besides,the changes of serum CRP,LDH,Alb and β2 -MG were compared.Results In the treatment group,the ORR was 90.63%,CR was 37.50%,VGPR was 25.00%,PR was 28.13%.And in the controlled group,the ORR was 62.06%,CR was 10.34%,VGPR was 17.24%,PR was 34.48%.The further studies shows that the CR and ORR between the two groups were significantly different (χ2 =6.050,7.015,P=0.013, 0.008).Moreover,the side effects included hematological toxicities and non-hematological toxicities which were not different notably (P>0.05).In addition,the serum CRP,LDH andβ2 -MG were decreased after the treatment nota-bly (t=7.321,4.972,7.668,5.174,4.231,2.379,all P<0.05),especially in the treatment group (t=-2.097,-2.409,-2.112,all P<0.05);the Alb was increased compared to precious notably (t=-4.170,-2.805,all P<0.05),especially in the treatment group (t=1.710,P<0.05).Conclusion The regimen of cyclophosphamide com-bined with MP is an effective and safe therapy for multiple myeloma which seems significantly superior to MP regimen with higher CR and ORR and which can also lower CRP,LDH,β2 -MG and raise Alb to cut down the burden of tumor.

Objective: To establish a colorectal cancer phage-peptide library and to screen for biomarkers for early detection of colorectal cancer. Methods: A T7 phage display peptide library was constructed using 30 surgical colorectal cancer specimens from Changhai Hospital, Second Military Medical University. Protein-A/G was used to enrich IgG from control sera as well as colorectal cancer sera. Five biopanning protocols were carried out for enrichment of colorectal cancer-specific phage clones, and 2 000 phage clones were randomly selected. ELISA was used for further screening of clones of different reactivities between the cancer serum and control serum; and the selected clones were subjected to DNA sequencing and the cloned protein function was forecasted by Chilibot for validation. Results: (1) The titer of the colorectal cancer phage display peptide library was 3.0×106pfu, with a recombination rate of 60% as showed by PCR identification and a storage capacity of 1.8×106 pfu. (2) Of the 18 phage clones selected by ELISA, 12 were cancer-related genes. Conclusion: ELISA for screening the recombinant tumor antigen phage display peptide library can be used to discover new differentially expressed antigens ; and the selected phage clones expressing antigen might be used for early detection of colorectal cancer.

Objective To explore the relationship of OLFM1 expression with the development of lung cancer by comparing the OLFM1 expression in lung cancer specimens and matched normal lung specimens. Methods The OLFM1 mRNA expression was examined by semi-quantitative RT-PCR and fluorescence quantitative PCR in 21 lung cancer tissues and the paired normal lung tissues, and the protein expression of OLFM1 was detected by immunohistochemistry. Results The OLFM mRNA expression levels in 8 primary lung squamous cell carcinoma tissues were decreased by 2. 2 folds compared with that in the matched normal lung tissues, with the difference being significantly different (P = 0.028). The OLFM1 protein was located in cytoplasm and only expressed in lung adenocarcinoma tissues ( P < 0. 001), and the expression was not associated with patients' age, gender, tumor stage, and differentiation degrees(P>0. 05). Conclusion The lower expression of OLFM1 gene in lung squamous cell carcinoma may serve as a potential molecule marker for the condition. The expression of OLFM1 protein in lung adenocarcinoma, but not in lung squamous cell carcinoma and normal lung tissue, may suggest that different pathological types of lung cancers may have different pathological mechanisms.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

Objective To discuss the clinical value of serum brain natriuretic peptide (BNP) ,tumor necrosis factor‐α(TNF‐α) , matrix metalloproteinase‐9(MMP‐9) and interleukin‐6(IL‐6) detection in chronic heart failure (CHF) patients with different heart function grades and their correlation with CHF .Methods Totally 67 cases of CHF in our hospital from Jan .2013 to Jun .2015 were selected as the observation group and the NYHA classification was performed :gradeⅠ in 15 cases ,gradeⅡ in 21 cases ,gradeⅢ in 23 cases and grade Ⅳ in 9 cases .Other 30 individuals undergoing the healthy physical examination were chosen as the control group at the same period .The venous blood samples were collected for detecting serum BNP ,TNF‐α,MMP‐9 and IL‐6 .LVEDD ,LVESD and LVEF were measured by echocardiography .Then the obtained data were analyzed by using the SPSS 21 .0 software .Results The serum BNP ,TNF‐α,MMP‐9 and IL‐6 levels in the observation group and various cardiac function groups were higher ,the differences between them were statistically significant (P< 0 .05);LVEF in the observation group and various cardiac function groups were obviously declined ,while LVEDD and LVESD were obviously increased ,the differences were statistically significant (P<0 .05) .The linear regression was adopted to analyze the correlation between serum BNP ,TNF‐α,MMP‐9 and IL‐6 with the left ventricular echocardiographic parameters in the CHF patients ,the results showed that serum BNP ,TNF‐α,MMP‐9 and IL‐6 levels were negatively correlated with LVEF (P<0 .05) ,while positively correlated with LVEDD and LVESD (P<0 .05) .Conclusion The serum BNP ,TNF‐α,MMP‐9 and IL‐6 levels are the good indexes for diagnosing CHF ,moreover can accurately reflect different heart function status in the CHF patients with different cardiac function grades ,which are closely related to the occurrence and de‐velopment process of CHF .

Altogether 202 cases of cervical spondylosis were treated with traction first,followed by needling such points as Jiaji (Ex-B 2), Dazhui (GV 14), Fengfu (GV 16), Fengchi(GB 20), Baihui (GV 20), Yuzhen (BL 9), Houxi (SI 3) and Hegu (LI 4) with combination of point injection on Jiaji (Ex-B 2) points and TDP irradiation on the affected region. After three courses of treatment, the total effective rate reached 98.5%, among which those with types of cervical, nerve-root and vertebral artery took 1-2 courses, while those with types of spinal cord and sympathetic nerve took 2-3 courses.

Objective Using second duplication checking to prevent academic misconduct of accepted papers ,and to improve the academic quality of journals for the consolidating credibility of academic journal and brand establishing .Methods Academic Mis-conduct Literature Check(AMLC)system developed by CNKI was applied to check possible academic misconduct of 90 accepted pa-pers for Chongqing Medical Journal ,then the contribution time ,duplication checking time ,words repetition percentage of the accept-ed papers with academic misconduct and similarity literature were analyzed compared with the source papers .Results After chec-king 90 accepted papers ,9 of them were suspected with academic misconduct ,including 5 papers with 30 .1% -40 .0% of coinci-dence rate ,1 paper with 40 .1% -50 .0% of coincidence rate ,and 3 papers with more than 50 .1% of coincidence rate .Most of the academic misconduct were multiple contribution of same manuscript or duplicate publication .Conclusion To avoid the multiple con-tribution of the same manuscript or duplicate publication ,we should shorten peer review and publishing period ,carry on multi-dupli-cation checking ,punish academic misconduct ,and work together with other sci-tech periodicals to eliminate academic misconduct .

Toxoplasmosis is an infectious disease which affects both human and animals.It distributes all over the world and makes great harm to human beings.In recent years,the morbidity of toxoplasmosis in our country increased because of contacting with pets.Toxoplasmosis has no specific clinical manifestations and responds well to correct treatment.In order to raise clinicians' notice to toxoplasmosis,this article will review the disease from the aspects of etiology,epidemiology,clinical manifestation and experimental diagnosis.