Chronic kidney disease ( CKD ) is a worldwide public health problem.Glomerular filtration rate ( GFR ) is a key indicator for early diagnosis and accurate classification of CKD , how to estimate GFR accurately and conveniently has been a difficulty and hot-points.We describes the development of several major eGFR equations based on creatinine and cystatin C , analysis the impact of the different research methods on performance of equation , and discusses the issue in research and application of eGFR in China.Accordingly make the following recommendations ( 1 ) Expand the study size ( multiple centers) and participants number , then develop and validate eGFR equations based on Chinese population ;(2) Standardize the gold standardof GFR in the study and unify the analysis and evaluation methods;(3) Promote the consistency and standardization of creatinine and cystatin C which is the basis for the wide range of applications of the eGFR formula.

OBJECTIVE:To bring the spillover effects of multinational pharmaceutical enterprises'Research&Develop-ment institutions intofull play in China.METHODS:The routes for the exertion of spillover effects and the main restrictive factors of multinational pharmaceutical enterprises were analyzed and some strategies to enhance the spillover effects were put forward.RESULTS&CONCLUSION:The absorbing ability of the domestic pharmaceutical enterprises should be enhanced in China;the flow of the personnel between the multinational enterprises and the domestic pharmaceutical enterprises should be accelerated.We should participate actively in the Research&Development system of multinational pharmaceutical enterprises,reinforce the protection of intellectual property and promote the Research&Development investment of multinational phar-maceutical enterprises in China.

OBJECTIVE:To discuss the outsourcing strategy of pharmaceutical enterprises.METHODS:The feasibility and the necessity of carrying out outsourcing strategy in pharmaceutical enterprises were analyzed based on value chain theory;and the current outsourcing strategies carried out in China pharmaceutical enterprises were evaluated.RESULTS&CON?CLUSION:Outsourcing is a feasible choice for our pharmaceutical enterprises,by which their competitive edge can be im?proved effectively.

Acupuncture Therapy ; Adult ; Female ; Humans ; Hyperhidrosis ; therapy ; Male ; Medicine in Literature ; Middle Aged ; Pruritus ; therapy

More and more studies have found that EBV infection is closely related to the occurrence, development, treatment and prognosis of lymphoma. The treatment of EBV-positive lymphoma bases mainly on combined chemo-radiotherapy together with ganciclovir, acyclovir and other antiviral drugs. Also there are novel ways to treat EBV positive- lymphoma including CD70 monoclonal antibody, butyrate, etc. The only way to prevent EBV infection is to inoculate anti-viral vaccine. The criterion of treating EBV positive-lymphoma remains to be further investigated.

0 05) The plasma Hcy level was significantly higher in DVT group than in control group [(12 2?8 7) ?mol/L vs (10 4?4 8) ?mol/L, P

Objective To explore the reversing mechanism of multidrug resistance of ciclosporin(CsA) on K562/A02 cell line,attenuating DNA damage repair through H2AX suppression.Methods K563 and K562/A02 respectively co-cultured with adriamycin(ADM) of different concentrations and CsA.MTT assay was employed to determine the inhibitory concentration of 50 percent(IC50),the resistance times and the reversal times.The K562/A02 cells treated with CsA,and reverse transcriptase(RT)-PCR technique was used to examine the mdr1 and H2AX mRNA level.Flow cytometry was used to measure P-glycoprotein(P-gp) and H2AX expression.Neutral comet assay was used to detect the level of DNA double strands break(DSBs) of the K562/A02 treated with ?H2AX antibody.Results 2?10-3g/L CsA could increase the sensitivity of K562/A02 to ADM,and the reversal times was 5.28.Mdr1 mRNA level and H2AX mRNA level were decreased when treated with CsA(P

Objective To propose a method for the determination of droxidopa concentration in human plasma by HPLC method for the study of the pharmacokinetics of droxidopa.Methods After a single oral dose of 200 mg droxidopa capsule was administered to 12 healthy Chinese male volunteers,we used perchloric acid to precipitate proteinum and then determined the droxidopa concentration in plasma by HPLC.The pharmacokinetic parameters of droxidopa were calculated by 3P87 software.Results The linear range was 0.025-2 ?g/ml.Pharmacokinetic parameters were: T1/2(108.50?30.78)min,AUC(185.74?26.41) ?g?ml-1?min-1,CL(1.08?0.08)ml/min,Tmax(99.90?10.05)min,Cmax(0.74?0.08)?g/ml.At 10 h after administration,droxidopa was eliminated from the blood almost completely.Conclusion The method we proposed is sensitive,reproducible,and easy to operate.Pharmacokinetics of droxidopa in vivo is fitted to two-compartment model.

Objective To investigate the best dose of treating diabetic renal insufficiency (DRI) with Tangduqing granules. Methods To establish model rats of DRI by the method of 5/6 nephrectomy and abdominal cavity injection with streptozotocin. The successful model rats with DRI were divided into four groups randomly:model group, high dose group, medium dose group, low dose group. Ten age-matched healthy male SD rats were served as controls (administered with NS 2 mL/d). Ten weeks later, contents of the BUN, SCr and urine protein excretion in 24 hours were determined. And to detect the best dose of treating DRI with Tangduqing granules by evaluating the effect of Tangduqing. Results Tangduqing had the function of reducing contents of BUN, SCr and urine protein excretion in 24 hours of rats with renal insufficiency. Conclusions Tangduqing may effectively delay the development of renal failure. The therapeutic effects of medium dose group were the best.

Thirty-six healthy women were divided into 3 groups according to their calcium intake at week 18 of gestation. The levels of their blood calcium, phosphate, parathyroid hormone (PTH) and calcitonin were assayed during pregnancy and postpartum, and bone mineral density ( BMD) was measured postpartum. The levels of PHI and calcitonin were increasing with advancing pregnancy and reached the highest at the end of pregnancy. The women with higher calcium intake during pregnancy period had higher BMD than that of the women with ordinary diet during pregnancy period.