1.Relationship between Wnt/?-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2
Journal of Chongqing Medical University 2003;0(06):-
Objective:To study the regulatory mechanism of Wnt/?-Catenin passthway-influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of ?-Catenin mRNA,Bcl-2mRNA,Caspase-8mRNA and Caspase-3mRNA.Methods:(1) Expressions of ?-Catenin,Bcl-2,Caspase-8 and Caspase-3mRNAs in groups with or without indomethacin induction were detected by RT-PCR.(2) Methyl thiazolyl tetrazolium(MTT) assay was used to measure the anticancer activity of indomethacin.Results:(1)As compared with groups without indomethacin induction,expression of ?-Catenin mRNA and Bcl-2 mRNA were lower,however,increased expression of Caspase-8 and Caspase-3mRNA were found in SGC-7901 cells after treatment with indomethacin.(2) Indomethacin of 50 ?mol/L at 24 h couldn't induce apoptosis of gastric cancer cells.Indomethacin of 50?mol/L at 48 and 72 h and of 100?mol/L and 200?mol/L at 24、48 and 72 h could induce apoptosis of gastric cancer cells in a dose-and time-dependent manner.Conclusion:Gastric cancer cells may be caused by apoptosis inbalance through Wnt/?-Catenin passthway,which activates expression of proto-oncogene Bcl-2 and inhibites expressions of Caspase-8 and Caspase-3.
2.Tuina Treatment for 37 Cases of Postoperative Urin ary Retention
Journal of Acupuncture and Tuina Science 2009;7(2):116-117
Objective:To observe the effect of postoperative urinary retention treated with tuina therapy.Methods:Tuina therapy was applied on 37 cases of postoperative urinary retention;Qihai(CV 6),Guanyuan(CV 4),Zhongji(CV 3)and bilateral Sanyinjiao(SP 6)were selected.Results:Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments.Conclusion:Point tuina is an effective method in treating postoperative urinary retention.
3.Exploration and practice on experimental teaching in preventive medicine specialty
Chinese Journal of Medical Education Research 2011;10(3):306-308
By reviewing experiment teaching,the primal problems and solutions of practical training in preventive medicine specialty are explored in order to provide some beneficial reference and help for experimental teaching.
4.Research progress on mitochondrial oxidative stress and corresponding targeted delivery systems in Alzheimer's disease
Ling-ling ZHOU ; Kang QIAN ; Peng YANG ; Qi-zhi ZHANG
Acta Pharmaceutica Sinica 2022;57(6):1630-1640
Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of
5.Impact of Conditioned Medium of Decidual Stromal Cell Cluture on Proliferation,Apoptosis and Invasion of Trophoblast Cells
Ling KANG ; Guowu YIN ; Xiaoming ZHU
Journal of Practical Obstetrics and Gynecology 2017;33(6):420-425
Objective:To investigate the impact of conditioned medium of decidual stromal cell culture(DSCM) on proliferation,apoptosis and invasion of trophoblast cells.Methods:lsolution,culturing and indentifying the purity of decidual stromal cells from healthy women of early pregnancy to prepare different concentrations of DSCM (5%,20%,50% and 100%);Proliferation was evaluated by MTT assay;Cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining;Cell invasion was assayed using a Transwell chamber;RT-PCR and gelatin zymography were used to explore the mechanism of cell invasion.Results:Using this method we obtained a purity over 90% of decidual stromal cells.With the concentration of DSCM increased(5%,20%,50%,100%),the OD value of the MTT assay has elevated and the rate of apoptosis declined,the infiltrating cell increased as well (P < 0.05);Compared with control group,DSCM treatment led to a significant increase in the mRNA and protein expression of MMP-2/9(P <0.05).Conclusions:Decidual microenviroment may promote trophoblast proliferation and inhibite its apoptosis besides could enhance its ability of invasion by altering the expression of MMP-2 and MMP-9.
6.Left ventricular multiple myxomas in a neonate.
Chinese Journal of Pediatrics 2005;43(8):630-630
7.Development of a new portable combined aid bag
Ling ZHANG ; Jianing LI ; Lingyan CHENG ; Jun KANG ; Ting ZHANG
Chinese Medical Equipment Journal 1989;0(03):-
Objective To study a new portable aid bag used to first aid and care in the process of wounded soldiers' delivery so as to meet the need of treatment in the high-technique local war. Methods The function,kinds and amount of outfit are decided on the basis of treating range. The new aid bag with hung sack and capsule outline is designed according to six different modules,which are not only easily-opened but convenient for fast treatment and supply. The field trial was taken after the development. Result A new portable combined aid bag fit for both wartime and peacetime is developed .Conclusion The new portable combined aid bag can meet the need of wounded soldiers' treatment in the modern high-technique local war,and can be used to first aid and care in the process of wounded soldiers' delivery.
8.Nosocomial Infection in Patients with Hematological Malignancies:Targeted Monitoring and Risk Factor Analysis
Hong ZHOU ; Ling REN ; Wen ZHENG ; Yiping MAO ; Haiquan KANG
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To investigate the characteristics and risk factors of nosocomial infection(NI) in patients with hematological malignancies(HM),and provide the bases for making the controlling measures of NI.METHODS Using the targeted monitoring to study NI in patients with HM,and recording 14 factors such as the time of hospitalization,chemotherapy,count of leukocyte and absolute neutrophil count(ANC) and so on.The data were analyzed with unifactorial ?2 test and multifactorial Logistic-regression analysis.RESULTS Among 242 patients with HM the prevalence of NI was 35.5%(86/242) and the prevalence of NI time-cases was 52.9%(128/242).Among 86 patients of NI there were 27 patients occurred multiple sites NI(31.4%).The main infection sites were upper respiratory tract,gastrointestinal tract,lower respiratory tract,oral cavity and blood.66.7% Of NI happened in the period of chemotherapy and 7 days after chemotherapy.The time of hospitalization and ANC were independent risk factors of NI in patients with HM.CONCLUSIONS The patients with HM are susceptible population of NI,and NI often occurs in the period of chemotherapy and 7 days after chemotherapy.So medical staff should strengthen monitoring,and shorten the time of patient hospitalization and of recovery of ANC to reduce the prevalence of NI efficiently.
9.Clinical Bacterial Distribution and Analysis of Drug Resistance in Lower Respiratory Tract Nosocomial Infection
Hong ZHOU ; Ling REN ; Fangzheng HAN ; Yiping MAO ; Haiquan KANG
Chinese Journal of Nosocomiology 2006;0(12):-
OBJECTIVE To analyze the bacterial distribution and drug resistance in lower respiratory tract nosocomial infection(NI).METHODS To investigate 351 patients suffered from lower respiratory tract NI using the prospective monitoring methods,and doing the pathogenic bacterium cultivation for sputums of 351 patients and then taking the susceptibility test.RESULTS Totally 346 pathogenic bacteria were found in sputums of 351 patients.The major pathogenic bacteria were Pseudomonas aeruginosa,Escherichia coli,Klebsiella and Staphylococcus aureus.ESBLs were 36.0% and 40.0%,respectively in E.coli and Klebsiella,and MRSA were 82.1% in S.aureus.Drug resistances were common in Gram-negative bacilli(GNB) and Gram-positive cocci.Piperacillin/tazobactam and cefoperazone/sulbactam and imipenem were the most sensitive for GNB,S.aureus,S.epidermidis and Enterococcus were all sensitive to vancomycin.CONCLUSIONS Drug resistance of the pathogenic bacteria in lower respiratory tract NI is common,so it′s necessary to emphasize pathogenic bacterium monitoring and use the antibacterials exactly.
10.New-type portable firstaid kit for evacuation corridor
Jianing LI ; Ting ZHANG ; Ling ZHANG ; Jun KANG
Chinese Medical Equipment Journal 1993;0(06):-
The paper mainly introuduces a kind of advanced portable firstaid kit,which is used in evacuation corridor by doctors,hygeians and nurses.It has such fuctions as hemostasis,bandaging,fixing,ventilation-improved respiration and contershock.With reasonable internal structure,it is very convenient to be quickly opened,closed and supplied.With the optimized allocation of ambulance drugs and materials,it meets the effective and modularization needs of field medical equipment.