1.Relationship between Wnt/?-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2
Journal of Chongqing Medical University 2003;0(06):-
Objective:To study the regulatory mechanism of Wnt/?-Catenin passthway-influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of ?-Catenin mRNA,Bcl-2mRNA,Caspase-8mRNA and Caspase-3mRNA.Methods:(1) Expressions of ?-Catenin,Bcl-2,Caspase-8 and Caspase-3mRNAs in groups with or without indomethacin induction were detected by RT-PCR.(2) Methyl thiazolyl tetrazolium(MTT) assay was used to measure the anticancer activity of indomethacin.Results:(1)As compared with groups without indomethacin induction,expression of ?-Catenin mRNA and Bcl-2 mRNA were lower,however,increased expression of Caspase-8 and Caspase-3mRNA were found in SGC-7901 cells after treatment with indomethacin.(2) Indomethacin of 50 ?mol/L at 24 h couldn't induce apoptosis of gastric cancer cells.Indomethacin of 50?mol/L at 48 and 72 h and of 100?mol/L and 200?mol/L at 24、48 and 72 h could induce apoptosis of gastric cancer cells in a dose-and time-dependent manner.Conclusion:Gastric cancer cells may be caused by apoptosis inbalance through Wnt/?-Catenin passthway,which activates expression of proto-oncogene Bcl-2 and inhibites expressions of Caspase-8 and Caspase-3.
2.Exploration and practice on experimental teaching in preventive medicine specialty
Chinese Journal of Medical Education Research 2011;10(3):306-308
By reviewing experiment teaching,the primal problems and solutions of practical training in preventive medicine specialty are explored in order to provide some beneficial reference and help for experimental teaching.
3.Tuina Treatment for 37 Cases of Postoperative Urin ary Retention
Journal of Acupuncture and Tuina Science 2009;7(2):116-117
Objective:To observe the effect of postoperative urinary retention treated with tuina therapy.Methods:Tuina therapy was applied on 37 cases of postoperative urinary retention;Qihai(CV 6),Guanyuan(CV 4),Zhongji(CV 3)and bilateral Sanyinjiao(SP 6)were selected.Results:Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments.Conclusion:Point tuina is an effective method in treating postoperative urinary retention.
4.Research progress on mitochondrial oxidative stress and corresponding targeted delivery systems in Alzheimer's disease
Ling-ling ZHOU ; Kang QIAN ; Peng YANG ; Qi-zhi ZHANG
Acta Pharmaceutica Sinica 2022;57(6):1630-1640
Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of
5.Left ventricular multiple myxomas in a neonate.
Chinese Journal of Pediatrics 2005;43(8):630-630
6.Impact of Conditioned Medium of Decidual Stromal Cell Cluture on Proliferation,Apoptosis and Invasion of Trophoblast Cells
Ling KANG ; Guowu YIN ; Xiaoming ZHU
Journal of Practical Obstetrics and Gynecology 2017;33(6):420-425
Objective:To investigate the impact of conditioned medium of decidual stromal cell culture(DSCM) on proliferation,apoptosis and invasion of trophoblast cells.Methods:lsolution,culturing and indentifying the purity of decidual stromal cells from healthy women of early pregnancy to prepare different concentrations of DSCM (5%,20%,50% and 100%);Proliferation was evaluated by MTT assay;Cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining;Cell invasion was assayed using a Transwell chamber;RT-PCR and gelatin zymography were used to explore the mechanism of cell invasion.Results:Using this method we obtained a purity over 90% of decidual stromal cells.With the concentration of DSCM increased(5%,20%,50%,100%),the OD value of the MTT assay has elevated and the rate of apoptosis declined,the infiltrating cell increased as well (P < 0.05);Compared with control group,DSCM treatment led to a significant increase in the mRNA and protein expression of MMP-2/9(P <0.05).Conclusions:Decidual microenviroment may promote trophoblast proliferation and inhibite its apoptosis besides could enhance its ability of invasion by altering the expression of MMP-2 and MMP-9.
7.Evaluation on safety and efficacy of tirofiban in patients with acute ST-elevation myocardial infarction undergoing emergency percutaneous coronary intervention
Liwei CHEN ; Ming YANG ; Kang GAO ; Yiguang SUN ; Ling HAN
Chinese Journal of Postgraduates of Medicine 2011;34(24):3-6
ObjectiveTo evaluate the safety and efficacy of tirofiba in the treatment of patients with acute ST-elevation myocardial infarction (STEMI) undergoing emergency percutaneous coronary intervention (PCI). MethodsA total of 158 patients with acute STEMI were randomly divided into tirofiban group 1 (59 cases, received tirofiban before PCI), tirofiban group 2 (56 cases, received tirofiban when PCI) and control group(43 cases, only received PCI). The coronary reperfusion flow(TIMI grade) of infarct related artery (IRA) after PCI, the resolution of the sum of ST segment elevation(sum STR) at 90 min after the procedure, the changes of myocardial enzyme at 6 h and 12 h afterwards, the left ventricular ejection fraction (LVEF) 1 week later, the major adverse cardiac events(MACE) within 30 d, bleeding and thrombocytopenia complications were analyzed and compared among the three groups. ResultsTIMI reperfusion grades in tirofiban group 1[98.3%(58/59 )]and tirofiban group 2[92.9%(52/56)]were higher than those in control group[60.5%(26/43)](P <0.05). The resolution of sum STR at 90 min after PCI in tirofiban group 1 [(89.3 ± 6.9)%]and tirofiban group 2[(82.4 + 7.3)%]was higher than that in control group[(65.6 +8.1 )%](P< 0.01 ),and there was significant difference between tirofiban group I and tirofiban group 2 (P<0.05 ). The occurrence of MACE within 30 d was lower in tirofiban group 1 and tirofiban group 2 than that in control group (P< 0.05). The level of CK-MB at 6 h and 12 h afterwards was lower in tirofiban group 1 than that in tirofiban group 2,and tirofiban group 2 was lower than control group (P< 0.05). LVEF 1 week later in tirofiban group 1[(56.2 + 6.4)%]was higher than that in tirofiban group 2[(51.1 + 4.9)%]and control group[(49.8 + 5.7)%](P < 0.05),but there was no significant difference between tirofiban group 2 and control group (P > 0.05). Although bleeding incidence in tirofiban group 1 and tirofiban group 2 was higher than that in control group, no severe bleeding and thrombocytopenia was observed. Conclusion Tirofiban can safely and effectively reduce the incidence of the ischemic events in the patients with acute STEM1 during preoperative of emergency PCI.
8.Review on the research progress of mining of OMIM data.
Jianhua LI ; Zheren LI ; Yan KANG ; Ling LI
Journal of Biomedical Engineering 2014;31(6):1400-1404
Online Mendelian Inheritance in Man (OMIM) is a knowledge source and data base for human genetic diseases and related genes. Each OMIM entry includes clinical synopsis, linkage analysis for candidate genes, chromosomal localization and animal models, which has become an authoritative source of information for the study of the relationship between genes and diseases. As overlap of disease symptoms may reflect interactions at the molecular level, comparison of phenotypic similarity may indicate candidate genes and help to discover functional connections between genes and proteins. However, the OMIM has used free text to describe disease phenotypes, which does not suit computer analysis. Standardization of OMIM data therefore has important implications for large-scale comparison of disease phenotypes and prediction of phenotype-genotype correlations. Recently, standard medical language systems, term frequency-inverse document frequency and the law of cosines for document classification have been introduced for mining of OMIM data. Combined with Gene Ontology and various comparison methods, this has achieved substantial successes. In this article, we have reviewed various methods for standardization and similarity comparison of OMIM data. We also predicted the trend for research in this direction.
Databases, Genetic
;
Humans
;
Phenotype
9.Epithelium constitution for esophageal tissue engineering using electrospinning technology.
Ling CHEN ; Jingjing LV ; Xuechan YU ; Cheng KANG ; Yabin ZHU
Chinese Journal of Biotechnology 2013;29(12):1796-1807
The basement membrane (BM) is crucial in regulating the physical and biological activities of esophageal epithelial cells which attach to the underlying BM. In order to simulate the natural construction of BM, we prepared the fibrous scaffolds using biodegradable polylactide (PLA) and silk fibroin (SF) as the materials via electrospinning technology. BM's proteins containing collagen (IV), laminin, entactin and proteoglycan were extracted from porcine esophagus and coated on the eletrospun fibers. Morphology, mechanical strength, biodegradability and cytocompatibility of the coated and uncoated scaffolds were tested and evaluated using scanning electron micrography, mechanical test system, immunofluorescence assay and western blotting with CK14 as the primary antibody. The fibrous scaffold PLA or PLA/SF, generated from the present protocol had good formation and mechanical and biodegradable properties. After coating with BM's proteins, the scaffold could enhance the growth and differentiation of esophageal epithelial cells, which would contribute to remodel and regenerate the tissue engineered epithelium and further contribute to engineer the whole esophagus in future.
Absorbable Implants
;
Basement Membrane
;
Biocompatible Materials
;
chemistry
;
Epithelium
;
Esophagus
;
physiology
;
Fibroins
;
chemistry
;
Humans
;
Nanostructures
;
chemistry
;
Polyesters
;
chemistry
;
Regeneration
;
physiology
;
Tissue Engineering
;
methods
;
Tissue Scaffolds
;
chemistry
10.An Investigation of influence of in-service training on awareness of tuberculosis prevention and detection in health-care workers
Suhua ZHENG ; Haiying LI ; Ying LING ; Wanii KANG ; Weiwei GAO
Chinese Journal of General Practitioners 2010;9(4):241-243
Objective To investigate awareness about tuberculosis (TB) prevention and treatment and influence of professional training on TB detection among health-care workers (HCWs) in general hospitals.Methods In total,750 HCWs were trained for TB-related knowledge for eight class-hours in a two-day course in three general hospitals,and 20 HCWs from each of the three hospitals classified as grade 3A and grade 2A in urban Beijing and grade B at suburban Beijing,respectively,were interviewed with questionnaire designed to understand their awareness about TB prevention policy,epidemiology,diagnosis and treatment,and to evaluate effectiveness of the training,respectively.All the trainees responded before and after the training,with a hundred percent of response rate.TB diagnosis and reporting one year before training in the three hospitals were compared to those one year after it.Results Scores of knowledge about TB diagnosis and treatment averaged 64-80 for HCWs before training,with statistically significant difference among three hospital (F = 5.984,P < 0.01).Scores increased after training,but without significant difference from those before it (P > 0.05).Awareness of TB prevention policy,regulations and epidemiology was insufficient in most HCWs of those hospitals,with lowest and highest average scores of 38.3 and 71.7 before training,respectively,but scores increased significantly after training (P < 0.01).Proportion of TB diagnosis with chest roentgenograph at grades 3A and 2A hospitals was significantly higher one month,three months and six months after training,as compared to that at suburban hospitals (P <0.01).There was significant decrease (P < 0.01) in proportion of chest roentgenograph at respiratory departments in hospitals grade 3A and grade 2A after training.There was no significant difference in reporting of pulmonary TB and positive sputum smear (P > 0.05) before and after training.Conclusions HCWs in general hospitals had experience and capacity in diagnosis and treatment of pulmonary TB,but their knowledge of TB prevention policy and epidemiology was insufficient.Their ability in finding and reporting TB can not been improved with short-term training.