ObjectiveTo explore the influence of early enteral nutrition (EEN) on the immune function and outcome in patients with severe traumatic brain injury.MethodsTotally 131 patients with severe traumatic brain injury who were admitted to the Intensive Care Unit of our hospital between March 2010 and March 2011 were enrolled in this study.Among them,69 were in EEN group and 62 were in control group.On day 7,humoral immune function was measured using automatic biochemistry analyzer,and the peripheral blood lymphocyte subset proportions were measured using flow cytometry.The complications were recorded in the initial 7 days after trauma.ResultsCompared with the control group,the EEN group had significantly lower incidences of complications including glucose metabolism disorder,diarrhea/stomach intestine distend,infection,multiple organ dysfunction syndrome,and stress ulcer on day 7 ( P =0.000,0.002,0.001,0.004,and 0.002).Hospital mortality in the EEN group was also significantly lower than that in control group ( P =0.028).The serum levels of IgM and IgG in EEN group were significantly higher than in control group ( both P =0.000),whereas the serum levels of C3 and C4 in the EEN group were significantly lower ( both P =0.000).IgA showed no significant difference between these two groups (P =0.144).The serum levels of CD3 + CD4 + T lymphocytes,CD4 +/CD8 + T lymphocytes,and CD19 + lymphocytes in the EEN group were significantly higher than in control group (all P =0.000),while CD3 + CD8 + T lymphocytes showed no significant difference ( P =0.070 ),The serum level of CD16+ CD56+ T lymphocytes in the EEN group was significantly lower than that in control group (P =0.026).ConclusionPatients with severe traumatic brain injury have good tolerance of EEN,which may enhance the immune function and improve the outcome of the patients.

Objective To investigate the display status of ultrasonography imaging check in central nervous system (CNS) in infants of early pregnancy and the diagnostic value of CNS malformation in infants of early pregnancy.Methods Gestational weeks of 2 751 enrolled subjects were divided according to the ultrasonic measurement of the crown rump length (CRL):11-11 +6 weeks group,12-12+6 weeks group,and 13-13 + 6 weeks group,prenatal ultrasound were performed to examine fetal CNS anatomy in infants of early pregnancy,record the display status in each groups of infants and analyze the relationship between the display situation and gestational age.Results Fourteen cases of fetal CNS malformation (20 malformations) in total were found by prenatal ultrasound,and the incidence of CNS malformation was about 5.09％ (14/2 571).Wherein,12 cases of early pregnancy were diagnosed,and 2 cases of middle pregnancy were diagnosed.The sensitivity of ultrasound of early pregnancy in the diagnosis of fetal CNS malformation was 85.71％.In the group of research,the ultrasound display ratios of 11-11+6 weeks group,12-12+6 weeks group and 13-13+6 weeks group were 96.73％,97.94％,98.06％,respectively.There was no significant difference in early pregnancy fetal CNS display ratio among groups (x2 =1.56,v =2,x2＜ x0.05.2 =5.99,P ＞ 0.05).Conclusions The display rate of CNS structure in infants of early pregnancy (11-13+6 weeks)is higher,and is not affected by gestational weeks.Prenatal ultrasound can effectively diagnose CNS severe malformation in infants of early pregnancy.

OBJECTIVETo obtain the normal value of Coben analysis for Haerbin with normal occlusion and analyze the different features of cranio-maxilla-facial structure between male and female, between adults and children.

METHODS120 Haerbin subjects (60 adults and 60 children) who fulfilled specific normal occlusion were seleceted from primary schools and universities students of Haerbin. They were divided into comparison groups on the basis of age and sex. Lateral cephalograms were taken for all subjects, and the mean and standard deviation for each parameter was recorded and compared between groups. Paired t test was used to assess statistical significance.

RESULTSIn depth, both sexes exhibited great increment in the middle and lower face. In anterior and posterior face height, the male adults exhibited increment, and in female adults only the posterior face height exhibited increment. The male adults face far exceeded the female in vertical growth, and less than the female in horizontal growth. No statistically significant differences of facial form were found between the sexes in children.

CONCLUSIONThe normal values of Coben analysis for Haerbin with normal occlusion was established. There were significant differences in normal values of Coben analysis between male and female, between adults and children.

Adult ; Cephalometry ; Child ; Dental Occlusion ; Face ; Female ; Humans ; Male ; Maxilla ; Vertical Dimension

Silk fibroin is a natural polymer with certain water solubility, structural modification, good biocompatibility and biodegradability, which can be used as a drug delivery carrier material. As a promising drug delivery system, drug-loaded silk fibroin nanoparticles can control drug release, reduce toxicity and improve therapeutic effects. In this paper, the basic characteristics of silk fibroin, the preparation methods of drug-loaded silk fibroin nanoparticles and the application of silk fibroin in nanoparticulate drug delivery systems are reviewed, and on this basis, the further development of drug-loaded silk fibroin nanoparticles is prospected.

Objective To examine the effects of comprehensive family intervention on family function among HIV-infected injection drug users (IDUs). Methods Ninety-eight HIV-infected IDUs in 3 AIDS treatment sites in Hunan Province were selected by random cluster sampling and were randomly divided into the intervention group (50 cases) and the control group (48 cases). Subjects in the intervention group were given a 9-month comprehensive family intervention, while those in the control group received standard treatment and care. When the study was completed, APGAR questionnaire was used to analyze the effects of comprehensive family intervention. Results Before the intervention ,the scores of family function were not significantly different the intervention group and the control group. After the intervention, the scores of family function among the control group were(4.26± 3.73) points and the intervention group was (6.53± 4.29) points, the scores of family function were significantly different between the two groups. Conclusions The comprehensive family intervention is an effective model to improve the family function of HIV-infected injection drug users.

Adolescent ; Biopsy, Fine-Needle ; Chromosomes, Human, Pair 18 ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Oncogene Proteins, Fusion ; genetics ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Sarcoma, Synovial ; genetics ; metabolism ; pathology ; Soft Tissue Neoplasms ; genetics ; metabolism ; pathology ; Translocation, Genetic ; Vimentin ; metabolism

Objective To investigate the biological behavior of breast cancer cells induced by miR-128 targeting RECK and its possible mechanism.Methods The expression of miR-128 in cell lines and transfection efficiency of lentiviral were detected by Western blot.Flow cytometry was used to detect the effect of miR-128 on the apoptosis of breast cancer cells.The dual luciferase assay was used to detect the in-teraction of miR-128 and RECK in breast cancer cells.Flow cytometry was used to detect the reversal effect of RECK on miR-128 in the cell cycle and colony formation of breast cancer cells.Results The expression of miR-128 in MCF-7 cells was 3.05 times that of NC cells,and the lentiviral transfection efficiency was obvious.miR-128 inhibits the growth of MCF-7 cells by inducing apoptosis and inhibiting cell cycle progression.RECK was the direct target of miR-128,and miR-128 directly regulated the expression of RECK.RECK ectopic recovery,miR-128 on breast cancer cell apoptosis and cell cycle inhibition had also been partially restored.Conclusion miR-128 plays an important role in the development and progression of breast cancer.It can interact with RECK to regulate the biological behavior of breast cancer cells,which suggests that miR-128 and RECK may be the potential therapeutic targets of breast cancer.

OBJECTIVEBy analyzing the group variations of Chinese school-aged youth, to gather scientific bases for establishing a screening norm for obesity.

METHODSMore than 228,250 students aged 6 through 18 years participated in the 2000 National Surveillance on Students Health and Physical Fitness were enrolled as subjects, and were divided into various groups. The P(5), P(50) and P(95) of BMI were calculated and compared.

RESULTSThe differences of BMI among various groups were mainly reflected in the P95 high level of BMI, e.g., the means of BMI of boys aged 13 in large cities and in relatively underdeveloped rural areas were 25.7 and 21.1 with a difference of 4.6, and 22.8 and 20.3 for girls aged 11 with a difference of 2.5. Evident north-south differences were also found, especially during adolescent growth spurt. The means of BMI of those lived in the north and south China were 22.3 and 21.0 for girls aged 11 (difference 1.3), and 25.4 and 23.5 for boys aged 14 (difference 1.9), respectively. These findings clearly showed that much more obese youth exist in the metropolis than in the rural areas, and in the north than in the south China. The difference in BMI between the developed areas and developing areas could be found in early school ages. They were 22.5 and 16.7 for boys aged 7, and 19.8 and 16.7 for girls aged 7, respectively, which is a dominant difference among Chinese youth groups. However, that the BMI differences in the P(5) level were not as evident as in the high level means that malnutrition and under-weight were still popular in all Chinese youth groups.

CONCLUSIONIn making a national norm for screening obesity in China, we'd better take high-level growth population as reference, to adapt the secular growth changes of Chinese youth. However, the huge urban-rural, north-south, developed-developing differences shown in this study should also be taken into full consideration, in order to make the screen norm be accepted by most of the Chinese youth groups.

Adolescent ; Age Factors ; Body Mass Index ; Child ; China ; Female ; Geography ; Humans ; Male ; Mass Screening ; Obesity ; prevention & control ; Rural Population ; statistics & numerical data ; Urban Population ; statistics & numerical data

OBJECTIVETo observe the effect of recombinant human erythropoietin (rHuEPO) on immune function of premature rats.

METHODSRHuEPO of 250 IU/(kg.t) or 500 IU/(kg.t) was administered to premature rats every other day for nineteen days. The control premature rats were received normal saline. The changes of hemoglobin (Hb), serum erythropoietin (EPO), red blood cell (RBC) immune function, T lymphocyte proliferative responsiveness, and production of tumor necrosis factor alpha (TNF-alpha) were observed.

RESULTSPremature rats showed lower levels on Hb, RBC immune function, T cell responsiveness and production of TNF-alpha compared with mature rats at birth. The postnatal declines of Hb and RBC immune function were lessened in the treated groups of premature rats, the higher dosage group of 500 IU/(kg.t) was more significant than the lower dosage group of 250 IU/(kg.t). When experiments were over, Hb of control premature rats was (7.72 +/- 0.89) g/dl, Hb of premature rats received 500 IU/(kg.t) was (10.08 +/- 0.90) g/dl (P < 0.01). C3b-R% of control premature rats was (11.00 +/- 0.95)%, C3b-R% of premature rats received 500 IU/(kg.t) was (17.75 +/- 1.04)% (P < 0.01). IC-R% in control premature rats was (12.83 +/- 1.33)%, IC-R% of premature rats received 500 IU/(kg.t) was (10.50 +/- 1.67)% (P < 0.01). The postnatal rise of T cell responsiveness and the production of TNF-alpha in premature rats increased in the treated groups, which was more significant in the higher dosage group of 500 IU/(kg.t) than in the lower dosage group of 250 IU/(kg.t). The OD index of control premature rats was 0.159 +/- 0.014, the OD index of premature rats received 500 IU/(kg.t) was 0.354 +/- 0.050 (P < 0.01). TNF-alpha in control premature rats was (0.270 +/- 0.014) ng/ml, TNF-alpha of premature rats received 500 IU/(kg.t) was (0.415 +/- 0.010) ng/ml (P < 0.01).

CONCLUSIONS(1) Premature rats had lower RBC immune function and T cell responsiveness and underproduction of TNF-alpha at birth. (2) Premature rats had an improvement with the RBC immune function after rHuEPO administration. (3) Premature rats had improvements with T cell responsiveness and TNF-alpha after rHuEPO administration, and there was a positive correction between the RBC immune function and T cell responsiveness with the production of TNF-alpha.

Animals ; Erythrocyte Count ; Erythrocytes ; drug effects ; immunology ; Erythropoietin ; blood ; pharmacology ; Female ; Hemoglobins ; analysis ; Pregnancy ; Rats ; Recombinant Proteins ; T-Lymphocytes ; drug effects ; immunology ; Tumor Necrosis Factor-alpha ; analysis

To explore the association between polymorphisms of transcription factor 7-like 2 gene (TCF7L2) and type 2 diabetes mellitus in Hefei district. The results suggest that the genetic variation DG10S478 in the TCF7L2 gene was not associated with type 2 diabetes mellitus in Hefei district ( P>0.05 ). However, its attribution to the susceptibility of type 2 diabetes mellitus in Hefei is not important.