Diagnostic Errors ; Female ; Humans ; Maxillary Sinus ; pathology ; Melanoma ; diagnosis ; Middle Aged ; Paranasal Sinus Neoplasms ; diagnosis ; Polyps ; diagnosis ; pathology

Antibodies, Monoclonal, Humanized ; therapeutic use ; Bevacizumab ; Electrocoagulation ; methods ; Endoscopy ; Epistaxis ; therapy ; Hemostatic Techniques ; Humans ; Male ; Middle Aged ; Telangiectasia, Hereditary Hemorrhagic ; therapy

[Abstract ] Objective To investigate the molecular mechanism how genistein increased the sensitivity of colon cancer cell to 5-fluorouracil(FU)and promoted colon cancer cell apoptosis.Methods SW480 colon cancer cell line was chosen as experimental object.Genistein 80 μmol/L and 5-FU 30 μg/mL used separated or combined for 48 hours were set as drug experiment group.There were four experiment groups in this study:control group (without any drug),5-FU group,genistein group,5-FU and genistein combined group.The expression of survivin,Bcl-2,p21 ,caspase3 and caspase 9 in the tumor cells of each group at mRNA and protein level was deteced by real-time quantitative polymerase chain reaction (PCR) and Western blotting.The relative expression quantity of genes was determined by comparative threshold method (2 -ΔΔCT )andβ-actin was taken as an internal reference.Semi quantitative analysis was performed for protein relative expression quantity.The DNA combination activity of nuclear factor(NF)-κB of each group was measured by electrophoretic mobility shift assay (EMSA).Single factor analysis of variance was used for mean comparison among multiple groups and LSD test was for mean comparison between two groups.Results The expression of caspase3,caspase 9 and p21 of 5-FU and genistein combination group at mRNA (1 .903±0.122,2.726±0.050 and 2.541 ±0.393)and protein level (0.534±0.077,1 .161 ± 0.172 and 0.463±0.016)were all higher than those of control group (1 .001 ±0.052,1 .000±0.014 and 1 .001 ±0.037;0.080 ±0.043,0.248±0.059 and 0.139 ±0.021 ),genistein group (1 .559 ±0.038, 2.394±0.095 and 1 .686 ±0.061 ;0.335 ±0.052,0.478 ±0.059 and 0.304 ±0.018)and 5-FU group (1 .198±0.063,1 .051 ±0.043 and 1 .399±0.055 ;0.194±0.015 ,0.337 ±0.036 and 0.231 ±0.011 );the expression of survivin of 5-FU and genistein combined group at mRNA and protein level (0.165 ± 0.018 and 0.216±0.014)were all lower than those of control group,genistein group and 5-FU group (1 .001 ±0.033,0.775 ±0.044 and 0.395 ±0.030;0.594±0.079,0.375 ±0.014 and 0.295 ±0.014), and all the differences were statistically significant (gene,F = 802.865 ,52.760,39.992,187.288, 37.435 ;protein,F =10.466,44.483,19.490,200.011 ,45 .238;all P <0.01);the difference was also statistically significant in the expression of caspase3 and p21 of 5-FU group and genistein group compared with those of control group (LSD test,P <0.05 ).The results of EMSA assay showed that the DNA binding activity of NF-κB protein of genistein group (461.64 ±15.41 )and combined group (585.28 ±7.82) significantly decreased compared with that of control group (1 067.97 ±36.01)and 5-FU group (718.83± 23.18,LSD-test,P < 0.05).Conclusions Genistein combined with 5-FU seemed to have synergistic effects on apoptosis of colon cancer cell.The mechanism was that genistein inhibited the DNA binding activity of NF-κB mediated by genistein,further up-regulated caspase 3,caspase 9,p21 and down regulated survivin.Therefore,genistein may provide assistance in colon cancer chemotherapy.

Objective To investigate the effects of recombinant human augmenter of liver regeneration (rhALR) on renal inflammation in acute kidney injury (AKI) induced by renal ischemia reperfusion (IR). Methods SD rats were randomly divided into sham-operated group,IR group,rhALR1 group (100 μg/kg) and rhALR2 group (200 μg/kg).Both renal pedicles of rats were identified and occluded with microvascular clamps for 60 min to induce acute kidney injury (AKI).Blood urea nitrogen and serum creatinine levels were evaluated using a Hitachi 747 automatic analyzer. For histological examination, sections were stained with HE. The activity of myeloperoxidase (MPO) was detected by spectrophotometer.Expression of TNF-α,ICAM-1,MCP-1 was determined by Western blotting. Results Blood urea nitrogen,serum creatinine levels and the injury of kidney were improved significantly in rhALR group as compared with IR group (all P＜ 0.05).They were improved more significantly in rhALR2 group as compared to in rhALR1 group (all P＜0.05).The protein levels of TNF-α,ICAM-1,MCP-1 and the activity of MPO in kidneys from the sham-operated rats were low,and increased significantly after renal ischemia reperfusion injury (all P＜0.05).After treated with rhALR,the expression of TNF-α,ICAM-1,MCP-1 and the activity of MPO were decreased significantly in kidneys as compared to those in IR group (all P＜0.05),which decreased more significantly in rhALR2 group than those in rhALR1 group (all P＜ 0.05). Conclusions nhALR can protect kidneys from ischemia reperfusion injury in rats.The mechanism may be associated with the inhibition of renal inflammatory cells infiltration and down-regulated expressions of YNF-α,ICAM-1 and MCP-1 in the kidney.

This study is to establish the gastric cold model of rats. After gastric feeding with cold water for 5 weeks and extra iced water bath in the last 2 weeks, model group show distinct physical sign of gastric cold syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group(treated with Fanzuojin Wan) show mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than blank group. The difference with significance among model group, treatment group and blank group reveals the success of the establishment of gastric cold syndrome.
Animals ; Cold Temperature ; Disease Models, Animal ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach ; chemistry ; metabolism ; pathology ; physiopathology ; Stomach Diseases ; metabolism ; pathology ; physiopathology

This study is to establish the gastric hot model of rats. After gastric feeding with ethanol solution for 3 weeks and feeding with extra capsaicin and ethanol solution for another 2 weeks, model group show distinct physical sign of gastric hot syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group (treat with Zuojin Wan) shows mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than the blank group. The obvious difference among model group, treatment group and blank group reveals the success of the establishment of gastric hot model.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Eating ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach Diseases ; drug therapy ; pathology ; physiopathology

Calcinosis ; Fibula ; Free Tissue Flaps ; pathology ; Humans ; Reconstructive Surgical Procedures

Objective:To assess the feasibility and safety of 2-staged hybrid technique for treating coronary artery disease (CAD) patients with multi-vessel lesions.
Methods: Our research included 2 groups:Hybrid group, CAD patients with left anterior descending artery (LAD) lesion or with other major epicardial vessel stenosis>70%who received 2-staged hybrid treatment in our hospital from 2012-03 to 2015-03 and Control group, CAD patients received elective conventional off-pump coronary artery bypass (OPCAB) by the same surgeon at meanwhile. n=91 in each group. The peri-operative conditions and complications were compared between two groups.
Results: Compared with Control group, Hybrid group had the shorter post-operative mechanical ventilation time (7.9 ± 4.8) h vs (21.6 ± 35.9) h, shorter ICU-stay time (29.6 ± 20.8) h vs (47.5 ± 38.3) h, all P<0.01 and less peri-operative blood transfusion (0.59 ± 1.48) U vs (2.82 ± 3.81) U, P<0.01. The post-operative complications of mortality, MI occurrence and delayed wound healing were similar between 2 groups, P>0.05.
Conclusion:2-staged hybrid technique is a safe, feasible and minimally invasive technique for treating CAD patients with LAD and multi-vessel lesions.

Objective To evaluate the effectiveness of transsphenoidal endoscopic endonasal approach for the resection of Rathke cleft cysts (RCC).Methods Thirty-five pathologically diagnosed RCCs were resected throuth transsphenoidal endoscopic endonasal approach at the HuanHu hospital between January 2000 and December 2011.The clinical data were retrospectively analysed.There were 12 male and 23 female.The average age was 45.7 years and the average disease course was 4.6 years.The typical clinical manifestations included headache (26 cases),visual interference (11 cases) and pituitary dysfunction (8 cases).The postoperative symptoms and follow-up results were recorded.Results All of the patients underwent complete removal of the cyst contents,complete removal of the cyst wall in 20,fenestration and aspiration of the RCC with partial excision of the cyst wall in 15.The mean cyst volume was (1 145.3 ± 12.1) mm3 [range (121-4586) mm3].One patient had cerebrospinal fluid leak,which was repaired using artificial dura mate of brain and gelatin sponge intraoperatively.No postoperative complications were found.Follow-up time ranged from half a year to 11 years.Headache resolved in 100％ (26/26),visual interference resolved in 90.9％ (10/11),pituitary dysfunction resolved in 62.5％ (5/8).No patient recurred.Conclusion It is concluded that the transsphenoidal endoscopic endonasal approach is microinvasive,safe and effective in the treatment of RCC.

Objective To analyze the correlation between olfactory bulb (OB) volume with depth of olfactory sulcus(OS) and olfactory function in patients with idiopathic olfactory loss(IOL).Methods Forty patients with IOL and forty normal controls were compared in terms of olfactory function T&T testing and magnetic resonance imaging (MRI,observation of OB volume and depth of OS).T&T testing and MRI were performed again after a year in 40 IOL patients,the results were compared with the first time.Results OB volume of left side in IOL patients was (30.31 ±4.07) mm3,right side was (30.82 ±4.14) mm3,average OB volume was (30.53 ±4.10)mm3 ; OB volume of left side in normal controls was (49.56 ±7.19)mm3,right side was (49.84 ±7.25)mm3,average OB volume was (49.73 ±7.21)mm3.OB volume was lower in IOL patients as compared to controls(t value were 8.122,8.274,8.231,all P ＜ 0.01).OS depth study revealed no statistical different between IOL patients and controls(t value were 0.998,1.017,1.001,all P ＞ 0.05).Olfactory discriminate threshold was negatively correlated with OB volume in IOL patients (r =-0.53,P ＜ 0.05).There was no correlation with the depth of OS(r =-0.19,P ＞ 0.05).Among 40 IOL patients,when followed-up,12 showed increased in OB volume and olfactory function after a year,but no statistical difference was found with the first time (t value were 0.831,0.864,0.826,all P ＞ 0.05).The other 28 patients showed no significant changes of OB volume and olfactory function.Conclusions The OB volume was lower in IOL patients as compared to normal controls.The depth of OS showed no significant changes in IOL patients.The OB volume was correlated with olfactory function.The depth of OS did not correlated with the olfactory function.Some IOL patients showed increased OB volume and improved olfactory function with the development of the disease.