1.Manual reduction for radius head fracture with radioulnar synostosis and elbow disloction: a case report.
He-bo LIU ; Ling-li WEI ; Chang-bao ZHOU
China Journal of Orthopaedics and Traumatology 2015;28(6):535-537
Adult
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Elbow Joint
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injuries
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Female
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Humans
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Joint Dislocations
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therapy
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Musculoskeletal Manipulations
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Radius
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abnormalities
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Radius Fractures
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therapy
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Synostosis
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therapy
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Ulna
;
abnormalities
2.Critical care medicine in China: history and perspective.
Ling LIU ; Da-Wei LIU ; Hai-Bo QIU
Chinese Medical Journal 2013;126(10):1806-1808
3.The effects of curcumin on selenic cataract in rats
Wan-guo, FENG ; Bo, FU ; Ling-ling, DU ; Wei-qi, GAO
Chinese Journal of Experimental Ophthalmology 2011;29(11):983-986
Background Curcumin can eliminate free radical arising in the system and superoxide anion and further inhibit lipid peroxidation.Researches proved that curcumin has resisting effect on oxidative damage of lens,but its effect on the formation of age-related cataract is under clear.Objective This study was to investigate the effect of curcumin on the cataract induced by sodium selenite.Methods Thirty healthy clean SD rats of 12-day-old were selected.All the rats were randomly divided into the normal control group,model group and curcumin group and ten rats for every group.The selenite cataract models were established in the rats of model group and curcumin group by the injection of sodium selenite subcutaneously.Meanwhile,0.005 % curcumin lavaged the rats in the curcumin group daily for two weeks.Lens of the rats were examined under the slit lamp at 4,7,10,14 days after injection in all groups,and the opacification of lens was graded based on the He' criteria.The lenses of rats were obtained at the end of experiment and then the level of superoxide dismutase (SOD),the activities of malondialdehyde (MDA) and glutathione peroxidase(GSH-Px) in rat lens were detected using biochemical assay method.The use of experimental animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The lenses were transparent throughout the experimental duration in the blank control group.Compared with model group,the development of the Ⅲ,Ⅳ,Ⅴ grade of cataract was slow down in the curcumin group with the significant difference( P<0.05 ).The content of MDA,SOD and GSH-Px activity in lens appeared considerable differences among 3 groups ( MDA:F =215.42,P<0.01 ; SOD:F =46.83,P< 0.01 ; GSH-Px:F=44.29,P<0.01 ).The level of SOD was significantly reduced in the model group and the curcumin group compered with blank control group ( P < 0.05 ) and that in the curcumin group was higher than the model group ( P<0.05 ).The MDA level in the model group and the curcumin group was obviously rised in comparison with blank control group( P<0.05 ),but that in the curcumin group lowed in comparison to model group( P<0.05 ).GSH-Px activity in the curcumin group was significantly lower than that in blank control group and higher than that in the model group( P < 0.05 ).Conclusions The curcumin can effectively delay rather than the cataract formation induced by sodium selenite in SD rat by improved the resistance of oxidation in the lens.
4.Effect of fibrous root extract of Coptis chinensis on soil microbes and enzyme activities.
Yang-Bo LI ; Lin-Wei HE ; Wei ZHANG ; Ye-Kuan WU ; Ling YUAN ; Jian-Guo HUANG
China Journal of Chinese Materia Medica 2014;39(21):4205-4210
Coptis chinensis is widely used as Chinese medicine herbs and serious soil problems occur after continual cultivation of this medicinal plant. In the preset experiment, fibrous root extract of C. chinensis (REC) was added into soil to study the effect of REC on microbes and enzyme activity in soil. The results showed that both bacteria and actinomycetes decreased by about 2 times in contrast to fungi, which increased by about 3 folds. Phosphorus bacteria, potassium bacteria, azotobacter, ammonia bacteria, and nitrifying bacteria were also reduced significantly by REC, suggesting the inhibition of nitrogen biofixation and supply, mobilization of phosphorus and potassium, ad plant growth promotion as REC added into soil. There were multiple influences of REC on soil enzyme activities. Invertase activity was stimulated, while urease was inhibited and dehydrogenase unchanged by REC, indicating the interference of biochemical reactions in soil. In addition, type and total content of phosphorus lipid fatty acids (PLFAs) , the signature of microbes, decreased while the ratio of bacterium to fungus PLFAs increased as REC increased in soil, which suggested that fungi increased relatively with bacteria decreased thereby leading to easy occurrence of crop fungus diseases following cultivation of C. chinensis. The decrease in diversity and evenness indexes of microbial community in soil by REC indicated soil ecosystem deterioration and reduction of microbial groups and densities in soil. Therefore, allelopathic chemicals released from the roots of C. chinensis could change microbial community structure and resulted in serious soil problems by continual cropping of this medicinal plant.
Coptis
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Ecosystem
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Plant Extracts
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pharmacology
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Plant Roots
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Soil
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chemistry
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Soil Microbiology
5.Atypical ossifying fibromyxoid tumor: a case report and literature review.
Yuan HUANG ; Huan-Jin LOU ; Wei-Bo MAO ; Wei GONG ; Yi-Ling ZHU
Chinese Journal of Pathology 2008;37(3):206-207
Aged
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Female
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Fibroma
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pathology
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Fibroma, Ossifying
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pathology
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Gingival Neoplasms
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pathology
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Humans
6.Hepatic lymphoepithelioma-like cholangiocarcinoma: report of a case.
Wei-bo MAO ; Wei GONG ; Yuan HUANG ; Shao-jie XU ; Yi-ling ZHU ; Zhong-wei ZHAO
Chinese Journal of Pathology 2011;40(7):493-494
Adult
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Bile Duct Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Bile Ducts, Intrahepatic
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Carcinoma, Hepatocellular
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metabolism
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pathology
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Carcinoma, Squamous Cell
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diagnosis
;
metabolism
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pathology
;
surgery
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Cholangiocarcinoma
;
diagnosis
;
metabolism
;
pathology
;
surgery
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Cholecystectomy
;
methods
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Diagnosis, Differential
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Hepatectomy
;
methods
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Humans
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In Situ Hybridization
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Keratin-19
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metabolism
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Keratin-7
;
metabolism
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Keratin-8
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metabolism
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Magnetic Resonance Imaging
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Male
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RNA, Viral
;
metabolism
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Tomography, X-Ray Computed
7.Effect of pigment epithelium derived factor on NO and the expression of caspase-3 in retinal tissues of model rats with optic nerve crush injury
Xiao-Xiao, YAN ; Hai-Bo, JIA ; Xiao-Ling, YIN ; Cui, CUI ; Wei-Xing, PU ; Nan, HUO ; Jun-Bo, ZHAO
International Eye Science 2017;17(6):1047-1050
AIM: To analyze the effect of pigment epithelium derived factor (PEDF) on nitrogen monoxide (NO) and expression of cysteine-containing, aspartate-specific proteases-3 (caspase-3) in retinal tissues of model rats with optic nerve crush injury.METHODS: A total of 60 SD rats were randomly divided into the blank control group, model group and PEDF group, with 20 rats in each group.Except the blank control group, the optic nerve crush injury rat models were established in the other groups, and left eyeballs were taken as samples.After successfully modeling, the model group were treated with intravitreal injection of 5μL of balanced salt solution while PEDF group were treated with intravitreal injection of 5μL of PEDF (0.2μg/μL).Two weeks later, the retinal tissues were collected, and changes of shape were observed under microscope after HE staining.The changes of NO level were measured by colorimetry assay, the expression of caspase-3 mRNA and caspase-3 protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blot.RESULTS: HE staining showed that retinal tissues of the blank control group arranged neatly and clearly.Retinal ganglion cells (RGCs) arranged in a monolayer, and cells were oval, uniform in size and distribution, the cell nuclei were clear, closely arranged, with clear boundaries.The retinal tissues of the model group were sparse in shape, RGCs showed vacuolar changes, the overall number of cells was reduced, and cell nuclei of residual RGCs showed pyknosis and uneven staining.RGCs in PEDF group were with slightly edema and arranged closely, and the degree of injury was significantly milder than that in the model group.Levels of Caspase-3 mRNA and protein and NO levels in the three groups showed the model group > PEDF group > blank control group (all P < 0.05).CONCLUSION: The application of PEDF can down regulate the expression of Caspase-3 and NO in rates with optic nerve injury and reduce RGCs injury.
10.Effect of alcohol exposure during pregnancy on learning and memory and expression of cdk5 in the hippocampus of infant rats.
Shuang LI ; Chun-Yang XU ; Hong-Bo JIANG ; Wei HAO ; Rui-Ling ZHANG
Chinese Journal of Applied Physiology 2012;28(1):13-83
Animals
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Cyclin-Dependent Kinase 5
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metabolism
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Ethanol
;
adverse effects
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Female
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Hippocampus
;
drug effects
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metabolism
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Learning
;
drug effects
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Male
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Memory
;
drug effects
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Pregnancy
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Prenatal Exposure Delayed Effects
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metabolism
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physiopathology
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Rats