Objective To explore the changes of humoral immunity in neonates with hypoxic-ischemic encephalopathy(HIE)and observe the influence of perinatal factors on humoral immune function.Methods Sixty-two neonates with HIE were enrolled and 30 healthy neonates were selected as control group.The percentages of CD19,CD25 of B lymphocyte were detected by Flow Cytometr.The levels of IgG,IgM,IgA and complement C3,C4 were detected by immune rate nephewlometry.The results were analyzed by SPSS 11.5 software.Results 1.The percentages of CD19+(17.93?3.10)%,CD19+CD25+(0.64?0.42)% and the levels of IgM(0.13?0.05)g/L,IgA(0.14?0.07)g/L and complement C3(0.62?0.12)g/L,C4(0.10?0.03)g/L in neonates with HIE were significantly lower than those in the healthy term neonates(Pa0.05).2.There were dignificant diffe-rences of the percentages of CD19+,CD19+CD25+ and the levels of IgM,IgA,complement C3,C4 among the different stages of HIE(Pa

Objective To observe the sub-chronic effects of low doses of arsenic poisoning in rabbits exposed to different periods on some of the serum enzymes and biochemical indicators, and to provide the basis for screening of meaningful hematologic indicators for early diagnosis of arsenic poisoning. Methods Twelve adult rabbits,weighing 2.0 - 3.5 kg, were randomly divided into four groups, 3 in each group, and they were fed with drinking water containing sodium arsenite 0(control),0.01,0.05,0.25 mg/L, respectively. Serum alanine aminotransferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), γ-glutamyl transacylase (y-GT), total protein(TP), albumin(ALB), globulin(GLP), and ALB/GLP of rabbit were measured by SYSMEX-180 automated biochemistry analyzer after 8 weeks and 12 weeks exposure. Results The results showed that ALT in 0.05 mg/Lgroup of 12 week[(60.00 ± 4.14)U/L]increased significantly compared with the control[(41.50 ± 2.12)U/L, P ＜0.05];AST in 0.25 mg/L group of 8 week and 12 week[(46.50 ± 3.21 ), (52.33 ± 3.81 )U/L]increased significantly compared with the control[(21.33 ± 3.53), (29.50 ± 3.23 )U/L, all P ＜ 0.05];ALP in 0.05 mg/L and 0.25 mg/L group of 12 week [(78.68 ± 4.85 ), ( 103.00 ± 7.83 ) U / L]increased significantly compared with the control [(45.50 ± 5.50)U/L, all P ＜ 0.05];γ-GT in 0.05 mg/L group of 12 week[(19.33 ± 7.50)U/L]increased significantly compared with the contro1[(8.50 ± 3.53)U/L, P＜ 0.05]. TP, ALB, GLP, ALB/GLP of different groups of 8 week and 12 week were not significantly different statistically(F= 0.77,0.02,0.16,3.14 and 0.51,0.29,0.41,0.52, all P ＞ 0.05). Conclusions Zero point zero five mg/L and higher doses of sub-chronic arsenic exposure has some major damage to the liver. Compared with other serum enzymes and the biochemical indexes, serum AST is a early sensitive indicator of liver injury of the arsenic poisoning.

OBJECTIVETo investigate the effects of the treatment of supplementing qi, nourishing yin, and promoting blood flow (SQNYPBF) on the serum levels of CRP, TNF-alpha, IL-1 and IL-6, as well as the expression of HLA-DR in the peripheral monocytes in septic patients suffering from stress-induced hyperglycemia.

METHODSIn the prospective randomized controlled study, eighty-five stress-induced hyperglycemia patients with sepsis were randomly assigned to the experimental group (45 cases) and the control group (40 cases). On the basis of routine therapies, including anti-infection, nutrition support, and the glucose control with insulin pump, patients in the experimental group additionally received the treatment of SQNYPBF (They were intravenously dripped with Shenmai Injection and Sulfotanshinone Sodium Injection, once daily, for 7 successive days). The serum levels of CRP, TNF-alpha, IL-1, and IL-6 and the HLA-DR expression of the peripheral monocytes were detected using ELISA before treatment and on the 8th day of the treatment. The total dose and the duration of insulin used, the morbidity of hypoglycemia, the APACHE II scores, and the mortality within 28-day hospitalization were compared between the two groups.

RESULTSThe total dose of insulin used, the duration of insulin used, the morbidity of hypoglycemia, the APACHE II score on the 8th day of treatment, and the mortality within 28-day hospitalization significantly decreased in the experimental group, when compared with the control group (P < 0.05, P < 0.01). There was no difference in the expression of HLA-DR, the serum levels of CRP, TNF-alpha, IL-1, or IL-6 before treatment between the two groups (P > 0.05). After treatment the serum levels of CRP, TNF-alpha, IL-1, and IL-6 significantly decreased (P < 0.05) and the expression of HLA-DR significantly increased in the two groups (P < 0.05). Better effects were shown in the experimental group (P < 0.05, P < 0.01).

CONCLUSIONSQNYPBF combined intensive insulin therapy could better improve the sepsis patients' immunity, decrease the plasma glucose level and duration, increase their survival rate, and improve their prognosis.

APACHE ; Aged ; C-Reactive Protein ; analysis ; Female ; HLA-DR Antigens ; metabolism ; Humans ; Hyperglycemia ; drug therapy ; etiology ; Interleukin-1 ; blood ; Interleukin-6 ; blood ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Oxidative Stress ; Prospective Studies ; Sepsis ; complications ; drug therapy ; metabolism ; Tumor Necrosis Factor-alpha ; blood

Nowadays, nanotechnologies have shown wide application foreground in the biomedical field of medicine laboratory tests, drug delivery, gene therapy and bioremediation. However, in recent years, nanomaterials have been labeled poisonous, because of the disputes and misunderstandings of mainstream views on their safety. Besides, for the barriers of technical issues in preparation like: (1) low efficacy (poor PK & PD and low drug loading), (2) high cost (irreproducibility and difficulty in scale up), little of that research has been successfully translated into commercial products. Currently, along with the new theory of "physical damage is the origin of nanotoxicity", biodegradability and biocompatibility of nanomaterials are listed as the basic principle of safe application of nanomaterials. Combining scientific design based on molecular level with precision control of process engineering will provide a new strategy to overcome the core technical challenges. New turning point of translational medicine in nanotechnology may emerge.
Biocompatible Materials ; Nanostructures ; toxicity ; Nanotechnology ; Translational Medical Research

Objective To study the influence of fluoride on the expression of osteoprotegerin(OPG) mRNA and protein in suckling rat osteoblasts. Methods Osteoblasts obtained from calvarial of suckling Wistar rats were cultured in vitro in the media supplemented with NaF at a series of doses[O(control), 1,2 and 4 mg/L groups], and OPG mRNA expression and protein were evaluated by RT-PCR and ELISA methods, respectively. Results OPG mRNA expression in suckling rat osteoblasts cultured in vitro significantly increased after exposure to NaF for 48 h and 72 h(F=333.48,808.34,P<0.05). OPG mRNA expression in suckling rat osteoblasts cultured in vitro after exposure to NaF for 48 h at different doses(0.810±0.003, 0.819±0.031 and 0.870±0.044 for 1,2 and 4 mg/L groups, respectively) compared with that of control (0.800±0.040, all P<0.05). OPG mRNA expression further increased for 72 h exposure to NaF(0.933±0.047,1.031±0.051,1.240±0.062 for 1,2 and 4 mg/L, respectively), significantly higher than that of the control (0.805±0.020,all P<0.05) and corresponding groups at 48 h. NaF doses and time exposure exhibited a significant synergistic effect on OPG mRNA expression(F=2004.16, P<0.05). NaF also enhanced OPG protein expression in suckling rat osteoblasts cultured in vitro. Significant differences were observed only in 4 mg/L group(0.228±0.014,0.277±0.048) and control(0.205±0.012,0.229±0.010) at 48 h and 72 h (P<0.05). In addition, OPG protein expression at 72 h post-exposure was higher than that at 48 h,but there was no synergistic effect between concentration and time(F=1.21,P>0.05). Conclusions The results suggested that NaF could increase OPG mRNA and protein expression in suckling rat osteoblasts with a synergistic effect between the doses and exposure time.

Objective:To explore the gene differential expression pattern of polycystic ovary syn-drome.Methods:We carried out microarray analysis to define the gene networks by the PCOS granulosacells in order to identify differentially expressed genes in PCOS patients.These granulosa cells of fivePCOS cases and five control cases which were derived during oocyte retrieval from women undergoingIVF.Results:As compared with control human ovarian granulosa cells,46 genes were screened out,25genes were up-regulated,and 21genes were down-regulated in PCOS.These differentially expressedgenes were involved in various biologic functions,such as regulation of fatty acid metabolism,cell-cellsignal transduction,immune and inflammatory response,reflecting the complexity of clinical manifesta-tions of PCOS.Conclusion:Microarray analysis technology is an effective mothod to identify novel PCOSassociated candidate genes.

Objective To investigate the minimally invasive treatment of complex renal calculi nursing.Methods Retrospective analysis,from February 2009 to January 2011,50 regular channels percutaneous nephrolithotomy nephrostomy lithotomy patients(observation group)and from January 2007 to January 2009,the traditional opening of the regular 50 surgical treatment of complex renal calculi patients(control group)were selected,and length of stay,hospital costs,complications such as postoperative infection and other indicators were observed between the two groups.Results Successful rate of percutaneous nephrostomy large channel lithotomy in the observation group were 100％.Days in hospital(t =2.95,P ＜0.05),costs(t =11.68,P ＜0.05)and complication rate after operation(x2 =6.25,P ＜ 0.05)were significantly different between the two groups.Conclusions Comprehensive patient evaluation and preoperative psychological communication,close observation and meticulous condition postoperative care,help to promote patient recovery and improve the quality of care.

Objective To explore the nursing care for complications of children with complex renal calculus who receive minimally invnsive percutaneous nephrolithotomy lithotripsy treatment.Methods Children who received routine minimally invasive percutaneous nephrolithotomy hthotripsy treatment for complex renal calculus from February 2008 to April 29,2011 and 30 cases who received conventional open surgery from February 2005 to 2008 were divided into the observation group and the control group,respectively.Postoperative hospital stay,hospital costs and the incidence of postoperative complications were compared between the two groups.Results Hospital stay in observation group was(12.80 ± 2.60)d,and(18.25 ± 3.34)d in control group; there was statistically significant difference between the two groups(t =10.32,P ＜ 0.05).Hospital costs in observation group was(13 924.9 ± 340.3)yuan,and(15 510.4 ± 509.3)yuan in control group,the difference was obvious(t =13.20,P ＜ 0.05)between the two groups.the incidence of postoperative complications was 10.3％ and 40.0％ in observation group and in control group,respectively,there was significant difference(x2 =6.60,P ＜ 0.05).Conclusions Minimally invasive percutaneous nephrolithotomy was better than traditional open surgery in terms of dealing with children with complex stone stones,close observation,careful and meticulous care and more records,more communication can help reduce the number of hospital stay,hospital costs and the incidence of postoperative infectious complications,etc.It is good for the rehabilitation of patients and improvement of the quality of care.

Objective To explore and evaluate the effect of family intervention on quality of life of children with tourette syndrome.Methods Total of 200 children with tourette syndrome were randomly divided into observation group and control group.The observation group received conventional treatment and family intervention including family education,family visits and education guidance to build a harmonious family environment and healthy lifestyle,while the control group only received the conventional treatment and care.The therapeutic effects of both groups were assessed by Yale Global Tic Severity Scale (YGTSS),PedsQLTM 4.0 universal core scales score and Children' s subjective quality of life questionnaires.Results One year later,the frequency,intensity,complexity,interference,damage of spasm in the observation group were significantly lower than those of the control group (t =14.370,20.612,17.677,24.386,11.643,13.972,respectively;P ＜0.01 ).The average scores of families report in the observation group in physical function,mental function,social function,role function were (90.78 ± 11.32 ),( 88.80 ± 14.43 ),( 76.78 ± 11.30 ),( 66.80 ± 14.53 ),respectively,which were higher than (80.10 ± 14.23 ),(58.10 ± 13.32),(58.50 ± 14.32),(52.80 ± 13.15 )in the control group,and the differences were statistically significant (t =5.873,17.563,10.677,7.139,respectively;P ＜ 0.01 ). Each dimension score of children self-evaluation in the observation group was significantly higher than that in the control group (P ＜ 0.001 ).There were significant differences in family life,companion contact,life environment,self-cognition,depress experience,anxiety,except the dimension of emotional body( P ＜0.01 ).Conclusions Family intervention can significantly improve the quality of life and the quality of the population for children with tourette syndrome.

This study was purpose to investigate the effects of CD147 on the invasiveness of leukemia cells U937. The experiments were divided into 4 groups: control group, LPS group, CD147mAb group and LPS+CD147 mAb group. Cells were treated by lipopolysaccharide (LPS) or anti-CD147 monoclonal antibody, and the expression of CD147 and MMP-2, -9, the invasive potential of the cells in vitro and ex vivo, as well as the invasion of the implanted tumors in SCID mice were analysed by RT-PCR, FCM, gel zymography and invasion test in vitro respectively. The results showed that the expression of CD147 was elevated by the induction of LPS, and the enhanced expression of CD147 on U937 cells increased the production and secretion of MMP-2 and MMP-9 as measured by reverse transcription-PCR and gel zymography. An increased number of LPS-induced cells invading through a reconstituted basement membrane were observed by invasion assays. These responses were down-regulated after blocking CD147 with anti-CD147 antibody. At 30 days after intravenous injection of LPS pretreated U937 cells to SCID mice human U937 cells were found in the bone marrow and lung of the mice, indicating the invasion of the tumor cells. And overexpressions of CD147, MMP-2 and MMP-9 were found in the lung tissue of the mice injected with LPS-treated but not anti-CD147 antibody treated tumor cells. It is concluded that overexpression of CD147 on U937 cells may increase the secretion and activation of MMP-2 and MMP-9 and thus promote the invasiveness of the tumor cells.
Animals ; Antibodies, Monoclonal ; pharmacology ; Basigin ; genetics ; immunology ; metabolism ; Female ; Humans ; Matrix Metalloproteinase 2 ; genetics ; metabolism ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Mice ; Mice, Nude ; Mice, SCID ; Neoplasm Invasiveness ; RNA, Messenger ; genetics ; metabolism ; U937 Cells