Objective To develop a method for the determination of 13 kinds of organic phosphorus pesticides in the water by gas chromatography-mass spectrometry with solid phase extraction(SPE).Methods The 13 organic phosphorus pesticide residues,including dichlorvos,mevinphos,phorate,diazinon,propetamphos,ddimethoate,methyl parathion,malathion,fenitrothion,parathion,phenthion,methidathion,ethion,were separated on DB-1701P elastic silica capillary column followed by SPE with carbon-amino group complicated column and analyzed by gas chromatography mass spectrometry.Methylene dichloride was elution solvent,injection inlet temperature was 280 ℃ and column flow rate was 1.2 ml/min.Results The leaner range and limit of detection was 0.05-2.5 mg/L,0.49 ?g/L for dichlorvos;0.10-2.5 mg/L,1.4 ?g/L for mevinphos;0.05-2.5 mg/L,0.96 ?g/L for phorate;0.05-2.5 mg/L,0.73 ?g/L for diazinon;0.05-2.5 mg/L,0.60 ?g/L for propetamphos;0.05-2.5 mg/L,1.8 ?g/L for ddimethoate;0.10-2.5 mg/L,2.2 ?g/L for methyl parathion;0.05-2.5 mg/L,0.34 ?g/L for malathion;0.05-2.5 mg/L,1.1 ?g/L for fenitrothion;0.10-2.5 mg/L,0.58 ?g/L for parathion;0.05-2.5 mg/L,1.2 ?g/L for phenthion;0.05-2.5 mg/L,0.77 ?g/L for methidathion;0.50-2.5 mg/L,0.26 ?g/L for ethion.For all thirteen organic phosphorus pesticides,the correlation coefficient was above 0.99.The average recovery rates were 94.40%-100.80% and RSDs were found in the range of 4.17%-14.73%.Conclusion The method is simple,rapid,sensitive,accurate and applicable to the simultaneous determination of 13 kinds of organic phosphorus pesticide residues in water.

Objective To discuss the nursing care after the treatment of lower limb deep venous thrombosis by using thrombolytic drug from lateral dorsum of foot vein.Methods 32 patients with lower limb deep venous thrombosis were treated by using micropump pumping thrombolytic drug from lateral dorsum of foot vein,and administration of heparin sodium,706 artificial plasma and danshen root,in combination with nursing care,healthy guidance,observing the signs,symptoms and the effect of treatment.Results Of 32 cases,12 cases were complete recovery,19 cases were partial recovery and 1 case was noneffective.Conclusion Correct nursing care is important in the patients with lower limb deep venous thrombosis after thrombolytic treatment.

Objective To evaluate the effects of percutaneous balloon mitral valvuloplasty (PBMV) on repeated rheumatism attacks after continual anti rheumatic treatment in vain over a long period of time.Methods 23 cases received treatment by PBMV and follow up visits were performed.Results After operation,patients' cardiac function and their physique were improved obviously and the activity of rheumatism ceased after routine anti rheumatic treatment and prevention of streptococcal infection.Follow up visits for 36～81 months showed that mitral re stenosis occurred to only 2 patients.Conclusion Patients with mitral stenosis whose rheumatism attacks repeatedly in spite of longterm anti rheumatic treatment may be treated with PBMV first and then given anti rheumatism.In this way,a better curative effect can be obtained.

AIM: To establish a standard for quality control of Xinfang Biyan Capsule(Radix Saponikovae, Radix Angelicae Dahuricae, Radix Scutellariae, etc.). METHODS: TLC and HPLC were used. RESULTS: Flos Magnoliae, Radix Saponikovae, Flos Chrysanthemi, Radix Bupleuri in the capsule could be identified by TLC, and baicalin content in the capsule could be measured by RP-HPLC with a linear relatioship at a range of 0.243 ?g- 2.43 ?g(r= 0.999 8 ). The average recovery was 98.15% and RSD was 0.15% . CONCLUSION: The methods are accurate and can be used for the quality control of Xinfang Biyan Capsules.

Objective:To identify the effect of ?-synuclein overexpression on mitochondrial membrane structure with atomic force microscopy. Methods:?-syn expression was mediated by AAV (adeno-associated viral vector) and Recombinant AAV/?-syn and AAV/LacZ viral particles were stereotaxically injected in the left side of rat substantia nigra (SN) for rat model of ?-synuclein overexpression. Mitochondria were isolated from rats SN of Brain. Mitochondria were analysis with JC-1 staining,atomic force microscopy and Western blot. Results:By 16 weeks post-infection of AAV-?-syn,the level of ?-syn increased about 2 times in mitochondrial fraction with Western blot and mitochondrial membrane potential (??) decreased with JC-1 staining. Furthermore,mitochondria swelling and porous like structure formed on the mitochondrial membrane with atomic force microscopy. Conclusion:The data suggested that ?-syn could accumulate in mitochondria,might form mitochondrial membrane pores and lead to ?? decreases. ?-syn might lead to mitochondrial dysfunction in Parkinson's disease.

Objective To evaluate the feasibility and application of histopathology diagnosis of endometrial tissues obtained by endometrial cell collector (Jingyou, SAP-1). Methods One hundred and ninety-three patients whose endometrial lesions should be excluded were selected. First, endometrial tissue were obtained from the patients by Jingyou, then they underwent comprehensive curettage under hysteroscopy. The histopathology diagnosis was performed respectively. The specimen satisfaction rate and diagnostic accuracy was analyzed and compared. Results The specimen satisfaction rate of curettage under hysteroscopy was 95.85%(185/193). The specimens of 8 cases were not satisfied because the tissues were not enough. The specimen satisfaction rate of Jingyou was 82.38% (159/193). The specimens of 34 cases were not satisfied, among whom in 10 cases scratches did not throughout the whole palace antrum, and in 24 cases tissues quality were poor. The endometrial thickness in unsatisfactory specimen by Jinyou was significantly thinner than that in satisfactory specimen:(0.64 ± 0.18) cm vs. (0.97 ± 0.43) cm, and there was statistical difference (P<0.05). The diagnostic accordance rate between Jingyou and curettage under hysteroscopy was 79.87%(127/159). The sensitivity of Jingyou from high to low was 94.19% (81/86) in normal menstrual endometrial, 7/10 in endometrial carcinoma/ atypical hyperplasia, 67.86%(38/56) in endometrial hyperplasia and 1/7 in endometrial polyps. Missed diagnosis of jingyou inluded 2 cases of endometrial carcinoma and 4 cases of endometrial atypical hyperplasia. The misdiagnosed rate of high grade endometrial lesions was 6/16, and the patients were misdiagnosed because the tissues were not enough. Four cases of endometrial atypical hyperplasia had underwent conservative treatment of repeated curettage. Conclusions Application of Jingyou can obtain micro endometrial tissues, and the accordance rate of histopathology diagnosis is high with curettage under hysteroscopy. When the collector makes a comprehensive collection to the uterine cavity specimen, it can accurately screen endometrial carcinoma and atypical hyperplasia. The patients who have endometrial atypical hyperplasia and receive conservative treatment and curettage repeatedly curettage, thin endometrium and ultrasonic highly suspected endometrial polyps, are not recommend to use Jingyou to obtain specimen. When the specimen is not satisfied using the collector, additional hysteroscopy should be performed to avoid misdiagnosis of high grade endometrial lesions.

Objective To observe the clinical effect and safety of umbilical cord blood mesenchymal stem cell(UCB-MSC) transplantation in the treatment of diabetic foot. Methods UCB-MSC suspension (cell concentration (3 -7) × 107/L,0.3 -0.5 ml per point) was injected into multiple spots on affected lower limb with a 3 cm × 3 cm istance among each point. Demixing injection could be performed in regions with multilayer muscles. Clinical symptoms and related index were routinely observed from the first day to three months after operation. Results After three months of UCB-MSC transplantation, pain of patients was relieved, skin temperature increased, intermittent claudication ameliorated, ulcer healed, ankle-brachial index and transcutaneous partial pressure of oxygen increased. The lower extremity lesions showed an abundant collateral vessel formation after the treatment in 2 patients by angiography. Both patients had no severe complications and adverse reactions, none underwent amputation. Conclusions Umbilical cord blood mesenchymal stem cell transplantation is a safe and effective treatment of diabetic lower limb ischemic disease, which can exempt the patient from amputation and improve their quality of life.

s of catheter,and extend the service life of a catheter.

Objective To investigate the effect of soybean isoflavone on histology and uhrastructure of benign prostatic hyperplasia in rats.Methods Male SD rats were injected subcutaneously testosterone propionate for 28 d to induce prostatic hyperplasia,and the rats were divided into 5 groups:control group,model group,and 3 test groups with SI in a dose of 60 mg/(kg·d),120 mg/(kg·d) and 240 mg/(kg·d),respectively.The wet prostate weight,prostatic index,morphological,uhrastmetural and morphometrie changes of the prostatic shndular and interstitial tissues were observed.Results The prostate wet weight,prostatic index and prostatic volumes in all dose groups were significantly lower than those in the models.In comparison with the model group,height of prostatic epithelial cells,glandular average diameters,volumes and surface areas in unit volume,as well as glandular circumferences,glandular relative total volumes and interstitial relative total volumes were all significantly decreased.Glandular counts,density,ratio of glandular surface area to volume,and glandular average curvature were all increased.Conclusions Soybean isoflavone can inhibit prostatic hyperplasia in rats.

OBJECTIVE:To provide scientific reference for rational,effective and economical drug use in pediatric depart-ment. METHODS:A total of 9771 prescriptions were randomly selected from children's branch of our hospital during May 2012-Apr. 2016. Those prescriptions were analyzed statistically in respects of drug type,prescription cost,utilization rate of injec-tion,utilization rate of essential medicine,clinical diagnosis,irrational drug use,use of antibiotics,etc. RESULTS:Among 9771 prescriptions,2.91 types of drugs were used in each prescription,and each prescription expended 77.10 yuan;utilization rate of in-jection was 57.11％,and that of national essential medicine was 67.80％. Respiratory tract disease was main disease (84.17％). There were 284 irrational prescriptions(2.91％),including 156 non-standard prescriptions,165 unsuitable prescriptions and 65 ex-traordinary prescriptions. Utilization rate of antibiotics was 18.25％,among which that of Cefoxitin sodium injection was the high-est(15.48％),but its utilization index was the lowest(0.58). The detection rate of microorganism isolated from bronchitis patients was in low level(45.10％). CONCLUSIONS:The rate of qualified prescription in the children's branch of our Hospital is higher than the requirement of the former Ministry of Public Health concerning the rate of qualified prescription >95％;utilization rate of antibiotics and injection are both in high level. In the future,it is necessary to strengthen prescription evaluation and promote stan-dard and rational use of drugs so as to guarantee safe,effective and economical use of drugs in clinic.