1.Clinical study of the correlation among the levels of human corticotropin-releasing hormone, c-Fos and connexin-43 at onset of labor
Xiang WU ; Yi-Ling DING ; Ling YU ;
Chinese Journal of Obstetrics and Gynecology 2000;0(11):-
Objective To investigate the variation of corticotropin-releasing hormone(CRH)in plasma,the mRNA and protein expression of c-Fos and gap junction gene connexin-43(Cx43)in the myometrium of term pregnancy women,and to study the correlations among CRH,Cx43 and c-Fos at onset of labor.Methods 30 cases in labor(L group),30 cases not in labor(NL group)but in term pregnancy, and 30 cases of non-pregnant(NP group)women undergoing hysterectomy due to cervical intraepithelial neoplasia were recruited into this clinical study.Radioimmunoassay was employed to measure the concentration of CRH in plasma;in situ hybridization assay and streptravidin-biotin peroxidase(SP) immunohistoehemical techniques were respectively used to detect the expression levels of c-Fos mRNA,Cx43 mRNA and the corresponding proteins.Results(1)The concentration of CRH in L group(81.8?11.9) pmol/L was significantly higher than that in NL group(34.5?18.6)pmol/L(P
2.INTRAUTERINE GROWTH RETARDATION AT FULL TERM PREGNANCIES WITH ENDOCRINE FACTORS
Ling WU ; Baoqin LIU ; Shuhua YU
Journal of Pharmaceutical Analysis 2000;12(1):73-76
Objective To investigate the relationship between intrauterine growth retardation (IUGR) and en docrine parameters so as to assess the effects of the main endocrine factors on IUGR. The concentrations of growth hormone (GH), insulin, T3, T4 and TSH were measured in umbilical cord blood, amniotic fluid and maternal serum. Methods The samples were collected from 23 pregnant women who were diagnosed as the full term IUGR, 42 normal full term pregnant women with normal infants' weight were taken as control. Growth hormone and insulin were mea sured by radioimmunoassay. T3, T4 and TSH were investigated by micro-radioimmunoassay. Results The concentra tions of growth hormone, insulin and T4 in umbilical cord blood were lower in IUGR than that in control group(GH 4. 63μg/L vs 7.01μg/L, insulin 10. 68μIU/ml vs 31.44μIU/ml, T4 87. 39nmol/L vs 138. 10nmol/L. P <0. 05, 0. 05 and 0. 05, respectively). The TSH concentration in umbilical cord blood was higher in IUGR than in control group (10. 84μmIU/L vs 5. 75μmIU/L, P <0. 01). The concentration of growth hormone in maternal serum and the concen tration of insulin in amniotic fluid were also lower in IUGR group than in control group(GH 1.77μg/L vs 2.74μg/L, P <0. 01, insulin 5. 84μIU/mi vs 15. 64μIU/ml, P <0. 01). Conclusion This study confirms that full term neonates with IUGR are abnormal in endocrine factors. The inadequacy of growth hormone may be one of the causes of IUGR. The relative scarcity of growth hormone and insulin seems to be a factor to compromise the fetus' metabolism. Be sides, the early hypothyrosis of infants with IUGR might protect them from unfavorable environment in the uterine.
3.Tissue culture and plantlet regeneration of Knoxia valerianoides
Zhengzhu LING ; Wenliu QIN ; Liying YU ; Zuzai LAN ; Qinghua WU
Chinese Traditional and Herbal Drugs 1994;0(10):-
Objective The tissue culture of the stem apex of Knoxia valerianoides was done to research the optimum culture medium for test-tube seedlings for large-scale planting.Methods The stem apex of wild K.valerianoides was used as the explants and cultivated in different culture media separately to induce fascicled-bud,root,and plantlet regeneration.Results The cultural medium MS+BA 2.0 mg/L+NAA 0.1 mg/L was suitable for the sub-multiplication of fascicled bud and the medium 1/2MS+NAA 1.0 mg/L for root inducement and the plantlet regeneration.The transplantation survival rate of the plantlet regeneration was 70%.Conclusion The tissue culture of K.valerianoides could be used to produce test-tube seedlings for large-scale planting.
4.The expression and significance of interleukin-9, interleukin-17 and interferon-γ in CD4+ T cells from patients with rheumatoid arthritis
Ling WU ; Jing LI ; Yu TANG ; Donghai ZHENG ; Haiyan YOU
Chinese Journal of Rheumatology 2013;17(9):616-619
Objective To detect the levels of IL-9,IL-17,and IFN-γ in CD4+T cells from patients with rheumatoid arthritis (RA).Methods The peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls were obtained,then the CD4+ T lymphocytes were tested by immunomagnetic beads.The protein levels of IL-9,IFN-γ and IL-17 were measured by flow cytometry (FCM).The mRNA levels of IL-9,IL-17,RORγt and IFN-γwere also detected by qRT-PCR.Data were analyzed by comparison between groups using variance analysis,and Pearson's correlation analysis was used for linear correlation analysis.Results The isolation of untouched human CD4+ T cells from PBMC was effective and its purity was over 90%.The protein levels of IL-9,IL-17,IFN-γwere higher in patients with active RA as compared with patients with inactive RA (P<0.01) which were (1.62±0.23)% vs (1.15±0.24)%(P<0.01),(1.47±0.20)% vs (1.04±0.26)%(P<0.01) and (8.1±0.6)% vs (6.9±1.0)%(P<0.01) respectively,so did the patients with RA when compared with healthy controls (P<0.01).The mRNA levels of IL-9,IL-17,RORγt and IFN-γ were higher in patients with active RA as compared with inactive RA patients (P<0.01),which were (3.0±0.6) vs (1.8±0.4) (p<0.01) (4.2±0.9)vs (2.3±0.7) (P<0.01),(4.1±0.7)vs (2.9±0.3) (P<0.01)and (4.0±0.8)vs (2.3±0.6) (P<0.01) respectively,so did the patients with RA when compared with healthy controls (P<0.01).Intracelluar IL-9 levels were positively correlated with IL-17 (r=0.632,P=0.001),IFN-γ (r=0.515,P=0.008),DAS28 (r=0.519,P=0.009) and ESR (r=0.857,P=0.038) but had no correlation with CRP (r=0.38,P=0.61).Conclusion The levels of IL-17,IL-9,IFN-γare higher in the PBMCs of RA patients,and these cytokines may participate in the pathogenesis of RA.
5.Experimental study the cardiomyogenic differentiation of bone marrow mesenchymal stem cell induced by 5-azacytidine in a "Cardiac-like" milieu
Xiang LING ; Jichun LIU ; Bentong YU ; Qicai WU
Clinical Medicine of China 2009;25(9):950-952
Objective To determine the effectivity of 5-azacytidine(5-Aza) inducing the cardiomyogenic SCs were isolated and purified from Sprague-Dawley (SD) rats. Some BMSCs were induced with 5-Aza,group Ⅰ was not induced with 5-Aza and the third generation of cells were used for the next experiment; group Ⅱ were cultured and induced with 5-Aza( 10 μmol/L) at the third day of the culture for 24 h; group Ⅲ were induced with 5-Aza( 10 μmol/L) for 24 h when the third generation of cell were fused; group Ⅳ were induced with 5-Aza( 10 μmol/L) for 24h at the third day of primary cuhure,which were induced with 5-Aza( 10 μmol/L) for 24 h before the third gener-phosis was found in the BMSCs among groups. CD34 was negatively expressed and CD29 and CD44 were positively gle 5-Aza inducing group(group Ⅲ ) was much higher than that in no 5-Aza inducing group( group Ⅰ ) [ MHC : (6. 82±2.05 ) % vs (3.61±1.14) % ], cTnI: (5.63±1.86) % vs (2.76±0.82) %, P < 0.05 ], but was significantly lower than the percentage in the double 5-Aza inducing group ( group Ⅳ ) [ MHC : ( 12.18±3.16) % vs ( 6.82±2. 05)% ] ,cTni:(9.93±2.79)% vs(5.63±1.86)% ,P<0.05]. Conclusions 5-Aza could promote the cardio-myogenic differentiation of BMSCs in a "Cardiac-like" Milieu. 5-Aza double induing is better than the single indu-cing.
6.The clinic outcome of CAD/CAM onlay in the restoration of molars with extensive defect after root canal treatment
Haiyue YU ; Dandan MA ; Xiaoyu LIN ; Zhiting LING ; Buling WU
Journal of Practical Stomatology 2017;33(1):41-44
Objective:To observe the clinical efficacy of CAD/CAM all-ceramic onlay in the restoration of molars with extensive defect after root canal therapy(RCT).Methods:260 molars with extensive defect were restored by all-ceramic onlay after RCT.The patients were followed up for 18 months.The restoration effects were assessed using USPHS criteria.Results:The success rate of CAD/CAM all-ceramic onlay was 98%.Restoration exfoliation was found in 3 cases,fractured in 2 cases.Conclusion:CAD/CAM all-ceramic onlay can be applied in restoring extensive postenor tooth-defection after RCT.
7.Observation on therapeutic effect of double unrelated umbilical cord blood transplantation for treatment of leukemia due to chronic benzene poisoning in adults.
Ling-zhen CHEN ; Jia-yu CHEN ; Jin-ming WU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(5):361-362
Adult
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Benzene
;
poisoning
;
Cord Blood Stem Cell Transplantation
;
Female
;
Humans
;
Infant, Newborn
;
Leukemia
;
etiology
;
surgery
;
Male
;
Treatment Outcome
;
Young Adult
8.The expression levels of three T-type calcium channel receptors in inner ear of C57BL/6J mice with age.
Chen PAN ; Yafeng YU ; Hongyang LING ; Wenying WU ; Gensheng XIAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(22):1791-1794
OBJECTIVE:
To explore the expression levels of three T-type calcium channel receptors (α1G; α1H; α1I) in the cochlea and spiral ganglion neurons of C57BL/6J mice with different ages.
METHOD:
Thirty cases of C57BL/6J mice were divided into three groups (6-8 W, 24-26 W, 42-44 W) according to the age. The expressions of three T-type calcium channel receptors were quantified by RT-PCR after hearing thresholds measured by ABR.
RESULT:
Three receptors were detected in the cochlea and spiral ganglion neurons of 6-8 W C57BL/6J mice. The quantitative results showed that the expression levels of α1H and α1I were highest among three receptors in spiral ganglion neurons and in the cochlea respectively. The expression levels of three receptors significantly decreased with age,especially at the age of 4244 W.
CONCLUSION
The expression of T-type calcium channel receptors reduced with age in the inner ear of C57BL/6J mice.
Animals
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Calcium Channels
;
Calcium Channels, T-Type
;
biosynthesis
;
Cochlea
;
metabolism
;
Ear, Inner
;
Mice
;
Mice, Inbred C57BL
;
Neurons
;
Receptors, Calcium-Sensing
;
Spiral Ganglion
;
metabolism
9.Russel Viper venom X effects on blood coagulation protein
Jun WU ; Xiu-Ling FENG ; Gui-Jie YU ; Zheng ZHANG ;
Chinese Journal of Laboratory Medicine 2003;0(07):-
Objective To study the effects of russel viper venom X(RVV X)on blood coagulation protein.Methods We divide diluted protein into control and RVV-X groups,then use chromogenic substract assay to detect the activation effect of RVV-Ⅹ on coagulation factor Ⅶ,Ⅸ,Ⅹ and antithrombin,plasminogen,with or without activator.Results In RVV-Ⅹ group,the coagulation factor Ⅶ, Ⅸ and plasminogen displayed weakly enhanced chromogenesis,all P
10.Electroencephalogram Non-linear Analysis on Menopause Depression Subjects
Bin YAO ; Dong-yu WU ; Ke ZHU ; Ling YIN
Chinese Journal of Rehabilitation Theory and Practice 2006;12(4):285-286
ObjectiveTo investigate the changes of non-linear dynamics characteristics of EEG of the patients with menopause depression under eyes closed state.MethodsEEG were recorded in 18 patients with menopause depression and 18 healthy volunteers under eyes closed state. Approximate entropy (ApEn) was calculated for all subjects.ResultsApEn of the menopause depression group at eyes closed state increased significantly compared with that of the healthy control group.ConclusionDynamic non-linear analysis is more appropriate for the study of mental functions changes of menopause depression.