Objective To observe clinical effects of compound Muniziqi granules combined with vitamin A acid cream, and analyze its mechanism of action.Methods 76 cases of patients with chloasma treated in our hospital in March 2010 to February 2014 were selected.They were divided into two groups according to random number table method, 38 cases in each group.Observation group was given compound Muniziqi granules( three times per day,orally) combined with vitamin A acid cream(smear once respectively each morning and evening),control group was taken vitamin C 0.3 g and vitamin E 0.2 g ( three times per day, orally ) comined with vitamin A acid cream ( smear once respectively each morning and evening ) treatment.Observed clinical effects, scores of lesion area and color, detected serum estrogen level in both groups by radioimmunoassay. Results Compared with pre-treatment lesion area and color scores of observation group significantly reduced ( P<0.05 ).Compared with control group post-treatment, lesion area and color scores of observation group significantly reduced(P<0.05).Clinical effective rates of observation group and control group was 94.74%and 75.31%, respectively, the difference was significantly between two groups(P<0.05).Compared with pre-treatment, serum E2, FSH, LH levels of observation group were remarkably reduced(P<0.05).Compared with control group post-treatment, serum E2,FSH,LH levels of observation group significantly reduced(P<0.05).Conclusion The clinical effect of compound Muniziqi granules combined with vitamin A acid cream on chloasma is significant.Its mechanism may be relate to levels of estradiol, follicle-stimulating hormone and luteinizing hormone.

OBJECTIVETo verify the clinical efficacy on chronic fatigue syndrome of qi deficiency syndrome treated with acupuncture at selective time and explore the effect mechanism.

METHODSEighty patients were randomized into a selective-time-acupuncture group and an acupuncture group, 40 cases in each one. Qihai (CV 6), Guanyuan (CV 4), Hegu (LI 4), Taichong (LR 3), Sanyinjiao (SP 6) and Zusanli (ST 36) were selected in the two groups. In the selective-time-acupuncture group, acupuncture was used at 9:00am to 11:00am. In the acupuncture group, acupuncture was used at any time except in the range from 9:00am to 11:00am. No any manipulation was applied after the arrival of needling sensation. The treatment was given once every day, 10 day treatment made one session and two sessions of treatment were required. The fatigue scale was adopted to evaluate the efficacy before and after treatment in the patients of the two groups. The ratios among CD3+, CD4+ and CD8+ T cells in the peripheral blood were detected before ad b a after treatment.

RESULTSIn the acupuncture group, the total score of fatigue and the score of physical fatigue were reduced after treatment as compared with those before treatment (all P<0.05). In the selective-time -acupuncture group, the total score of fatigue, the s core of physical fatigue and the score of mental fatigue after treatment were reduced obviously as compared with those hefore treatment (all P<0. 01). The improvements in the scores of the selective-time-acupuncture group were superior to the acupuncture group (all P<0. 05). The ratio of CD3+ and CD8+ T cells was increased obviously after treatment in the two groups (all P<0. 05) and the ratio of CD4+ and CD8+ T cells was reduced obviously in the selective-time-acupuncture group (P<0. 05), which was better than that in the acupuncture group (all P<0.05). The total effective rate was 95.0% (38/40) in the selective-time-acupuncture group, which was better than 80.0% (32/40) in the acupuncture group (P<0.05).

CONCLUSIONThe acupuncture therapy at selective time is effective in the treatment of chronic fatigue syndrome of qi deficiency syndrome, which is especially better at relieving mental fatigue. The effect of this therapy is achieved probably by improving the immune function via the regulation of the ratios among CD3+, CD4+ and CD8+ T cells.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Fatigue Syndrome, Chronic ; immunology ; therapy ; Female ; Humans ; Lymphocyte Count ; Male ; Middle Aged ; Qi ; T-Lymphocyte Subsets ; cytology ; immunology ; Treatment Outcome ; Young Adult

Objective To explore the level of sex knowledge of nursing undergraduates in theTraditional Chinese Medicine universities, so as to provide proper methods to carry out sex education.Methods 301 nursing undergraduates in Schiool of Nursing in Traditional Chinese Medicine Universities ofZhejiang were randomly selected from grade 2003,2004,2005, following knowledge, such as sex knowledge,sex experience, sex concepts, contraception knowledge, sex education, was investigated. Results Nursingundergraduates grasp better basic sex knowledge, 19. 3% of them experienced sex, 91.4% of themidentified the sexual behavior before marriage, however, the result showed worse in terms of contraceptionknowledge. Factors such as registered residence, charge and grade influenced the knowledge. ConclusionsWe should strength sex education for the female nursing undergraduates, those who are in the grade one andthree, study in private universities, live in the cities and countryside are the focus ones.

Objective To establish embryonic stem cells (ESC) that can express green fluorescent protein (CFP) and differentiate them into neurons. It would provide tagging neurons for clinical transplantation to cure neural system diseases. Methods ESC (R1) was transfeeted with a plasmid containing the GFP by electroporation. A transgeuic cell line was obtained after selection with G418. The ESCs were characterized by AKP staining. Monolayer differentiation method was used to induce neural differentiation derived from GFP-ESC and immunofluorescence method was used to identify Tuj1 positive cells. Results There was no significant difference(X2=3.14,P0.05) in transfect rates between liposome and electroporation (65% vs 79%). The AKP staining of GFP-ESC was positive. GFP-ESC could be differentiated into neural cells. Conclusions These results show that ESC expressing GFP has been estabhshed, which can be differemiated into neurons.

OBJECTIVETo investigate the inhibitory effects of OMT on TGF-β1-induced CFBs proliferation, and then explore the mechanism.

METHODThe experiment was randomly divided into 6 groups as following: control group (serum free DMEM), model group (20 μg x L(-1) TGF-β1), OMT low dose group (1.89 x 10(-4) mol x L(-1) + 20 μg x L(-1) TGF-β1), OMT medium dose group (3.78 x 10(-4) mol x L(-1) + 20 μg x L(-1) TGF-β1), OMT high dose group (7.56 x 10(-4) mol x L(-1) + 20 μg x L(-1) TGF-β1), SB203580 group (p38MAPK blocking agent, 1 x 10(-5) mol x L(-1) + 20 μg x L(-1) TGF-β1). Vimentin of CFBs was identified by immunocytochemical methods, α-SMA of myFBs as well. Inhibitory effects of OMT on CFBs proliferation was detected by the MTT assay. Picric acid Sirius red staining was analyzed collagen type I and collagen type III deposition. Western blot was determined the expression of p38MAPK, p-p38MAPK, collagen type I and collagen type III.

RESULTMTT results showed that OMT significantly inhibited CFBs proliferation induced by TGF-β1 (P < 0.01) α-SMA immunocytochemical experiments suggested that OMT could protect against the CFBs proliferation. OMT could significantly decrease the deposition of collagen type I and collagen type III by Western bloting and picric acid Sirius red staining. Western blot results showed that TGF-β1 enhanced p38MAPK phosphorylation, however OMT attenuated the phosphorylation of p38MAPK induced by TGF-β1 (P < 0.01).

CONCLUSIONOMT can inhibit the CFBs proliferation induced by TGF-β1, and its mechanism may be involved in inhibiting p38MAPK phosphorylation.

Alkaloids ; pharmacology ; Animals ; Cell Proliferation ; drug effects ; Collagen ; metabolism ; Down-Regulation ; Female ; Fibroblasts ; drug effects ; Heart ; drug effects ; In Vitro Techniques ; Male ; Phosphorylation ; Quinolizines ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Transforming Growth Factor beta1 ; antagonists & inhibitors ; p38 Mitogen-Activated Protein Kinases ; antagonists & inhibitors ; metabolism

Objective: To learn HIV related stigma and its associated factors among the patients on antiretroviral therapy ( ART ) in Wenshan Prefecture, Yunnan Province, so as to provide evidence for eliminating HIV discrimination. Methods: A total of 419 subjects were recruited by convenience sampling from three ART clinics in Wenshan City and Maguan County between October 2017 and January 2018. HIV/AIDS Related Stigma and Discrimination Scale developed by Li Xianhong et al was employed. The multivariate linear regression model were used to explore the influencing factors for HIV stigma. Results: The median scores of disclosure concern, public rejection, family stigma, internalized stigma, health service providers＇ stigma were 24.00, 6.00, 10.00, 20.00, 2.00, respectively, and the overall was 68.00. The multivariate linear regression analysis showed that female patients ( standardized β=0.135 ) , patients with opportunistic infection ( standardized β=0.120 ), patients had no HIV infected family member ( standardized β=-0.128 ) , patients without family support ( standardized β=-0.175 ) , patients received gift from ART clinics ( standardized β=0.124 ) , patients scored lower in ART knowledge ( standardized β=-0.117 ) were likely to scored higher in HIV stigma. Conclusions The stigma on disclosure concern and internalized stigma dimensions are grievous among ART patients in Wenshan Prefecture. Gender, opportunistic infection, HIV infection in family, family support, receiving incentive gifts from clinics and awareness of ART are associated with HIV stigma.

Objective To investigate nursing workload and related factors of Coronary Care Unit (CCU),and to provide evidence for rationally arranging nursing human resources and improving nursing efficiency.Methods 77 inpatients of a third-grade class-A hospital were surveyed by means of Intensive Care Nursing Scoring System (ICNSS) to investigate workload of CCU nurses.Results Total score of CCU nursing workload was (25.5714 ± 6.48) points.ICNSS scores of the four seasons were (27.24 ± 4.438 ),( 25.62 ±4.535),(22.33 ±9.403),(27.24 ±6.953 ),respectively,the difference was statistically significant (F =11.867,P ＜0.01 ).The LSD multiple comparison showed that there were significant differences of ICNSS scores between the first quarter and the second quarter,the first quarter and the third quarter,the second quarter and the third quarter,the third quarter and the fourth quarter,the difference was statistically significant (P ＜0.05 ).The workload of the nurses who took care of the patients admitted by means of wheel chair and stretcher was (20.74 ± 4.663 ) and (25.89 ± 6.508 ) points,and the difference was statistically significant ( t =0.319,P ＜0.05 ).There was significant difference of nursing workload regarding length of stay and percutaneous coronary intervention (P ＜ 0.05 ).Among same type of disease,workload of CCU nurses was the highest on admission and on the day of percutaneous coronary intervention.Nursing workload was found negatively correlated with length of stay of the patients ( P ＜ 0.05 ).Of the 36 patients undergone percutaneous coronary intervention,the workload of CCU nurses was (26.35 ± 4.057 ) scores on the day of intervention,(23.58 ±3.525) scores on day 1,( 22.16 ± 4.537 ) scores on day 2 and ( 19.17 ± 3.637 ) points after day3,the difference was statistically significant ( F =21.024,P ＜ 0.05 ).Conclusions A flexible staff arrangement should be adopted according to the characters of the disease,patients' conditions and the nursing workload.

AIM:To investigate the dependence of the adrenoceptor regulation on oxidative stress in the rats with cardiac injury induced by high sympathetic activity .METHODS: Healthy SD rats were randomly divided into 7 groups:control, model, propranolol (Pro), prazosin (Praz), Pro+Praz, vitamin E (VE) and Pro+Praz+VE.The rats were intraperitoneally injected with norepinephrine ( NE) for continuous 16 d to reproduce cardiac injury , and treated with the respective drugs .During the experimental process , the body weight was recorded .At the end of the experiments , the following parameters were measured:the ventricular remodeling indexes ( cardiac index and hydroxyproline of the left ven-tricle), histopathologic examination , oxidative/antioxidative indexes [MDA, SOD, catalase (CAT), GSH-Px and total an-tioxidant capacity (T-AOC)], and energy metabolism (Na+-K+ATPase and Ca2+-Mg2+ATPase).RESULTS: The in-crease of body weight in model group was significantly slower than that in control group after 9 d of treatment (P<0.05). The cardiac index and left ventricular hypertrophy were significantly increased .Oxidation/antioxidation and energy metabo-lism were disturbed.In Pro, Praz, Pro+Praz and VE groups, the body weight, cardiac index, left ventricular fibrosis and oxidative/antioxidative dysfunction were ameliorated .Pro, Praz and Pro +Praz increased the activity of Na +-K+ATPase and Ca2+-Mg2+ATPase.Treatment with Pro+Praz showed the best result in all of the indexes (P<0.05).CONCLU-SION:The dependence of adrenoceptor regulation plays an important role in the formation of oxidative stress in the process of rat cardiac injury induced by high sympathetic activity .

ObjectiveTo establish the reference intervals for ALT,AST,GGT and LDH among the Han nationality in Beijing.MethodsThe document C28-P3 issued by CLSI was a guideline about how to define,establish,and verify reference intervals in the clinical laboratory.IFCC had established multicenter enzymes reference intervals based on the guideline.Exclusion criteria were designed for screening candidate reference individual according to the document C28-P3 and the multicenter study's experience.Blood specimens were collected from 315 healthy individuals aged 20 to 60 years old,including 132 males and 183 females.Reference materials were used to ensure the accuracy of the test results of the four liver enzymes.The methods which used to test the four liver enzymes could be traced to the IFCC enzymes reference measure procedure,the reagent of ALT and AST included pyridoxal phosphate.Results There was statistically difference between males and females of the referenceranges forALT, ASTand GGT.Therefore,gender-specific reference intervals were established as ALT:8.2 -50.8 U/L (F),12.7 -71.8 U/L (M) ; AST:15.0 -36.7 U/L ( F),16.6 -51.1 U/L (M) ;GGT:9.0 -37.3 U/L (F),12.0 -50.9 U/L (M).For LDH,the reference interval was 127 -224 U/L,as no significant gender difference was found.ConclusionsThe reference intervals for the four liver enzymes based on the population of the Han nationality in Beijing are established.The upper reference limit for ALT in Beijing Han population is higher than that from other similar studies.

OBJECTIVE:To observe the effects of matrine in conjunction with vincristine(VCR),adriamycin(ADM)and diamminedichloroplatinum(DDP),respectively on the cell cycle of the KBV200drug-resistant cell line.METHODS:The cultured KBV drug-resistant cells were divided into control group,antitumor drug groups and the combination therapy groups(marine+antitumor drugs,respectively),FCM assay was used to detect and analyze the expression of cell cycle and apoptosis of each group.RESULTS:As compared with single antitumor drugs groups,no significant change was found in the proportion of cells and cell apoptosis in the combination therapy groups(matrine+VCR or matrine+ADM).But marine+DDD resulted in a reduction of the G 0 ～G 1 stage cell population but an increase of G 2 ～M stage cell population.CONCLUSIONS:It was assumed that matrine-induced reversal of KBV200cells'drug-resistant to VCR and ADM may not relate to cell cycle and cell apoptosis.Matrine can slightly enhance the cytotoxic effect of DDP.