1.Proteomic analysis of human ovarian cancer cell lines and their platinum-resistant clones
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
Objective To perform comparative proteomic analysis of human ovarian cancer cell lines for detecting platinum-resistance associated proteins.Methods The total proteins of two sensitive (SKOV3 and A2780)and four resistant(SKOV3/CDDP,SKOV3/CBP,A2780/CDDP and A2780/CBP) human ovarian cancer cell lines were separated by two-dimensional gel electrophoresis(2-DE).The differentially expressed proteins were analyzed using image analysis software,stained with Coomassie Brilliant Blue,then identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)and database searching.The mRNA and protein levels of the differentially expressed protein which was most significant in all of the four resistant cell lines were validated by RT-PCR and western blotting,respectively.Results Five proteins were found to be significant in four cell lines. Annexin A3 and destrin were up-regulated and nicotinamide-adenine dinucleotide phosphate(NADP)- dependent isocitrate dehydrogenase 1 was down-regulated in all the four resistant samples.Glutathione transferase omega 1 had an increased expression in the other three resistant cell lines except for SKOV3/CBP in which its expression was not changed.However,cofilin 1 represented a different trend.In the two resistant sublines of SKOV3,eofilin 1 had a down-regulation,but it had an up-regulation in the cell lines induced from SKOV3.The expression of annexin A3 was up-regulated by 3-20 fold and the results of RT- PCR and western blotting showed complete consistency with that by 2-DE.Conclusions Proteomic techniques are useful to the identification of the resistance-associated proteins in ovarian cancer platinum- resistant cell lines and five candidates have been found.The five differential proteins might become hopeful candidate biomarkers for resistance.
2.Evaluation of cervical intraepithelial neoplasia positive cutting edge after conization.
Zhi-qin DAI ; Ling-ya PAN ; Hui-fang HUANG
Chinese Journal of Oncology 2007;29(2):153-154
Adult
;
Aged
;
Cervical Intraepithelial Neoplasia
;
pathology
;
surgery
;
Cervix Uteri
;
pathology
;
surgery
;
Conization
;
methods
;
Female
;
Follow-Up Studies
;
Humans
;
Hysterectomy
;
methods
;
Middle Aged
;
Neoplasm Recurrence, Local
;
Neoplasm, Residual
;
pathology
;
surgery
;
Uterine Cervical Neoplasms
;
pathology
;
surgery
3.Ring chromosome 22 in a case.
Ling PAN ; Ya-Li SUN ; Chen-Ming XU
Chinese Journal of Pediatrics 2013;51(4):308-309
5.Evaluation of the follow up results of patients with cervical intraepithelial neoplasia Ⅲ after surgical treatment
Zhi-Qin DAI ; Ling-Ya PAN ; Hui-Fang HUANG ; Jing-He LANG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To evaluate the follow-up results of patients with cervical intraepithelial neoplasia Ⅲ(CIN Ⅲ)after surgical treatment.Methods A retrospective analysis of consecutive patients with CIN Ⅲ after surgical treatment between Jan 1st,1999 and Jun 30 th,2004 was performed.The follow- ups of the patients after surgical treatment were assessed.Results In the follow-up of patients with CINⅢ after surgical treatment,the rate of abnormal cytology was 9.3%.The rate of follow-up was higher in patients with cervical conization than in patients with initial hysterectomy and in patients of the oncological group than of the non-oncological group.The rate of follow-up was lower in patients over 40 years old.There was no difference in the residential areas of the patients.Conclusions Cytological follow-up of patients with CINⅢ after operation is varied.The rate of follow-up is lower in patients over 40 years old,in patients having initial hysterectomy and in patients of the non-oncological group.The rate of follow-up is associated with the knowledge about CIN of both surgeons and patients.
6.Clinical analysis of the effect of cervical conization on fertility and pregnancy outcome
Haojie HE ; Ling-Ya PAN ; Hui-Fang HUANG ; Jing-He LANG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(08):-
0.05).Among the 26 pregnancies,there occurred one ectopic pregnancy and four cases of spontaneous abortion.In the 13 delivery cases,there were one premature delivery,two cases of premature rupture of the membrane,and ten cases of cesarean section.The sample height of the cone was less than 2.0 cm in the nine delivery cases,and the mean width of the cone was over 2.5 cm.Conclusion No evidence of secondary infertility caused by cervical conization was found.There was also no significant increase in the number of either premature delivery cases or low birth weight infants. The sample height of the cone might play a more important role in the pregnancy outcome than the width, which still needs to be further verified by larger studies.
7.Role of high-risk human papillomavirus testing in the screening and management of cervical cancer precursors.
Acta Academiae Medicinae Sinicae 2007;29(5):691-696
Human papillomavirus (HPV) infection is an essential cause of cervical cancer. HPV testing therefore may maximize the clinical benefits of cervical screening and abnormal cervical cytology management. A negative HPV test in combination with a normal Pap test result in women age 30 years or older allows the safe extension of the cervical screening interval to 3 years. However, because HPV infection is common in young women and is usually transient, HPV testing is not recommended as part of primary cervical screening for women younger than 30 years. HPV testing is recommended for women of any age as a triage test with atypical squamous cells of undetermined significance (ASC-US) results and as an option for follow-up of women with HPV-positive ASC-US, atypical squamous cells "cannot rule out high-grade", low-grade squamous intraepithelial lesions, or atypical granular cells not found to have CIN 2/3. HPV testing is also recommended as an alternative to colposcopy and/or cytology for follow-up of treated cases. Proper use of HPV testing improves the management of women with cytologic abnormalities.
Age Factors
;
Cervical Intraepithelial Neoplasia
;
diagnosis
;
pathology
;
virology
;
Early Detection of Cancer
;
Female
;
Humans
;
Papillomaviridae
;
isolation & purification
;
Papillomavirus Infections
;
diagnosis
;
Precancerous Conditions
;
diagnosis
;
pathology
;
virology
;
Uterine Cervical Neoplasms
;
diagnosis
;
pathology
;
virology
;
Vaginal Smears
;
Virology
;
methods
8.Effect of points massage on the cerebral hemorrhage patients with constipation
Chinese Journal of Modern Nursing 2012;18(22):2699-2700
Objective To explore the prevention effect of points massage on the cerebral hemorrhage patients with constipation.Methods Totals of 160 cases of acute cerebral hemorrhage within 7 d were randomly divided into observation group (n =80) and control group (n =80).All cases received the routine care,and patients in observation group received the points massage.Then,the incidence of constipation of two groups,the blood pressure and heart rate of observation group before and after intervention were observed.Results The incidence of constipation after cerebral hemorrhage in observation group was 16.25% and that of the control group was 43.75%,and the difference was statistically significant (x2 =14.40,P < 0.01).Before and after intervention,the heart rate and blood pressure in observation group had no significant difference (t =0.21,-0.28,0.44 ;P > 0.05).Conclusions Points massage is benefit to patients for preventing constipation after cerebral hemorrhage.
9.High performance thin layer chromatography quality assessment of Rogor, methyl parathion, methamidophos and dichlorvos in serum.
Hui-fang YAN ; Ya-juan PAN ; Jian WANG ; Fu-gang ZHANG ; Xiao-shuang XIE ; Jing ZHANG ; Chun-ling LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(7):437-438
Animals
;
Chromatography, Thin Layer
;
Dichlorvos
;
blood
;
Dimethoate
;
blood
;
Insecticides
;
blood
;
Methyl Parathion
;
blood
;
Mice
;
Organothiophosphorus Compounds
;
blood
10.Opportunity of non-bladder perfusion after transurethral enucleation and resection of prostate
Hai-Yan SHEN ; Ya-Zhen LIU ; De-Ling PAN
Chinese Journal of Modern Nursing 2012;18(4):413-415
Objective To investigate the opportunity of non-bladder perfusion after transurethral enucleation and resection of prostate (TUERP).Methods Sixty patients suffered from TUERP were divided into two groups according to random number with 30 patients in each group.The observation group was halted bladder perfusion on the next day of operation,and the control group was halted after 3 d.The ambulation time,the rates of urinary tract infection and contraction of bladder and quality of life were recorded and analyzed.Results Contraction of bladder in 1 case and no urinary tract infection happened in the observation group and 9 cases and 10 cases in the control group respectively; the differences between the two groups were significant ( x2 =7.680,12.000,respectively ; P < 0.05 ).Score of QOL one month later in the observation group was (0.75 ± 0.15 ) and ( 1.50 ± 0.31 ) in the control group,and the difference was significant ( t =- 11.995,P <0.01 ).Conclusions The halted bladder perfusion in the next day after operation can decrease ambulation time,decrease the rates of urinary tract infection and contraction of bladder and improve quality of life.