1.microRNAs and lung cancer
Min XIANG ; Ling ZHU ; Yan CHEN ; Xin LIU ; Xicai WANG
Journal of International Oncology 2015;42(2):144-146
microRNAs (miRNAs) are short non-protein-coding RNAs,which play important roles in the cell proliferation,differentiation,apoptosis,as well as activation of oncogenic and antioncogenic signals.Researches show that the abnormal expressions of miRNAs are closely related to the tumorigenesis,histological type,diagnosis,treatment and prognosis of lung cancer.So miRNAs may be the most potential and promising therapeutic targets for lung cancer.
3.Clinical study on termination of second-trimester gestation using different doses of combined mifepristone-miso- prostol regimen
Tang-Ni QIN ; Rong-Xiang ZHOU ; Cheng-Ling SUN ; Qing-Hui ZHU ; Jin-Hu ZHU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To evaluate the using of either 225 or 150 microgrammes of mifepristone combined with misoprostol for termination of second-trimester gestation(16~24 weeks).Methods 180 women requesting voluntary induced abortion during gestation 16~24 weeks were randomised to three groups,group 1:oral mifepris- tone 225rag,group 2:oral mifepristone 150mg,and group 3:injected 100rag rivanot by amniocentestis.The total suc- cess rate,once success rate,the interval of having-medicine to uterine-constraction,the volume of bleeding within 2 hours after labour and cervical laceration rate were observed.Results The once success rate of induced labour in group 1 was higher than that in group 2 and group 3(P
4.Effects of liraglutide on eNOS and IRS-1 expressions in human umbilical vein endothelial cells cultured with high glucose
Ling YUE ; Jing DONG ; Guangda XIANG ; Linshuang ZHAO ; Junxia ZHANG ; Guangping ZHU ; Lin XIANG ; Min LIU ; Junyan LU
Chinese Journal of Endocrinology and Metabolism 2016;32(4):327-329
Humanumbilicalveinendothelialcells(HUVECs)weretreatedwith3nmol/Lliraglutidefor10, 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, and 270 minutes at the concentrations of 5. 5 or 30 mmol/L glucose. Western blot analysis was used to detected protein expression and phosphorylation of insulin receptor substrates-1 ( IRS-1 ) and endothelial nitric oxide synthase ( eNOS ) . The results showed that the baseline level of phosphorylated-eNOS/eNOS was lower in high glucose group than that in normal group(0. 239 ± 0. 016 vs 0. 400 ± 0. 02,P<0. 05). Liraglutide time-dependently increased phosphorylated-eNOS/eNOS and phosphorylated-IRS-1/IRS-1 levels at 5. 5 or 30 mmol/L glucose.
5.Detection and significance of fusion gene between TMPRSS2 and ETS transcription factor genes in fresh prostatic cancer tissues in Chinese patients.
Hua XIANG ; Zong-xin LING ; Ke SUN ; Guo-ping REN ; Qi-han YOU ; Xiong-zeng ZHU
Chinese Journal of Pathology 2011;40(3):187-188
Carcinoma
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genetics
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metabolism
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pathology
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surgery
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China
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Humans
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Male
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Oncogene Proteins, Fusion
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genetics
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Prostatic Hyperplasia
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genetics
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metabolism
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pathology
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surgery
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Prostatic Neoplasms
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genetics
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metabolism
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pathology
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surgery
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Proto-Oncogene Proteins c-ets
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genetics
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metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Serine Endopeptidases
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genetics
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metabolism
7.Synthesis and activity evaluation of PARP-1 inhibitors with azaindole skeleton.
Jie ZHOU ; Zhi-Xiang ZHU ; Xiao-Guang CHEN ; Bai-Ling XU
Acta Pharmaceutica Sinica 2013;48(12):1792-1799
PARP [poly(ADP-ribose)polymerase] represents a novel potential target in cancer therapy. It is involved in a DNA repair process by catalyzing the transfer of ADP-ribose units from NAD to a number of its substrate proteins. In this work, a series of novel azaindole derivatives was designed and synthesized. Moreover, 16 target molecules were screened and 8 compounds displayed inhibitory activity against PARP-1. It has been demonstrated that these azaindoles bearing cycloamine substituents at 2-position were active to both PARP-1 and PARP-2.
Antineoplastic Agents
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chemical synthesis
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chemistry
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pharmacology
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Aza Compounds
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chemical synthesis
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chemistry
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pharmacology
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Indoles
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chemical synthesis
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chemistry
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pharmacology
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Poly (ADP-Ribose) Polymerase-1
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Poly(ADP-ribose) Polymerases
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metabolism
8.Design, synthesis and biological evaluation of novel para-substituted 1-benzyl-quinazoline-2, 4 (1H, 3H)-diones as human PARP-1 inhibitors.
Hai-Ping YAO ; Zhi-Xiang ZHU ; Ming JI ; Xiao-Guang CHEN ; Bai-Ling XU
Acta Pharmaceutica Sinica 2014;49(4):497-503
Poly(ADP-ribose) polymerase-1 (PARP-1) has emerged as a promising anticancer drug target due to its key role in the DNA repair process. It can polymerize ADP-ribose units on its substrate proteins which are involved in the regulation of DNA repair. In this work, a novel series of para-substituted 1-benzyl-quinazoline-2, 4 (1H, 3H)-diones was designed and synthesized, and the inhibitory activities against PARP-1 of compounds 7a-7e, 8a-8f, 9a-9c and 10a-10c were evaluated. Of all the tested compounds, nine compounds displayed inhibitory activities with IC50 values ranging from 4.6 to 39.2 micromol x L(-1). In order to predict the binding modes of the potent molecules, molecular docking was performed using CDOCKER algorithm, and that will facilitate to further develop more potent PARP-1 inhibitors with a quinazolinedione scaffold.
Antineoplastic Agents
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chemical synthesis
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chemistry
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pharmacology
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Drug Design
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Enzyme Inhibitors
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chemical synthesis
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chemistry
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pharmacology
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Molecular Docking Simulation
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Molecular Structure
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Poly (ADP-Ribose) Polymerase-1
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Poly(ADP-ribose) Polymerases
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Quinazolinones
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chemical synthesis
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chemistry
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pharmacology
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Structure-Activity Relationship
10.Expression of proliferating cell nuclear antigen in renal tissues of children with primary nephrotic syndrome
zu-xiang, MA ; wei-ling, ZHAO ; xiao-jie, HE ; zhu-wen, YI
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To investigate the expression of proliferating cell nuclear antigen(PCNA) in renal tissues of children with primary nephrotic syndrome(NS),and elucidate the relationship between PCNA expression and cell proliferation in renal tissues from the children with primary NS.Methods Paraffin-embedded renal biopsy tissue sections from 39 patients with primary NS were examined by immunohistochemical staining with anti-PCNA monoclonal antibody,normal renal tissue sections from 6 nephrectomized patients with nephroma were selected as control. Possible correlation between the percentage of PCNA positive cells and the pathologic type , histopathological score, clinical indices (serum albumin ,serum cholesterol ,serum creatinine and 24 hours urine protein ) before renal biopsy of NS were evaluated separately .Results The percentage of PCNA positive cells in glomeruli and tubulom terstitium of NS patients was significantly higher than that of the control (P