Objective] The mechanism of astragalus polysaccharides treatment of asthma was discussed. [Methods]Through the domestic and foreign about traditional Chinese medicine monomer astragalus polysaccharide on IL-4, IL-10 and other cytokines and inflammatory infiltrates aspects such as the Th1/Th2 balance, smooth muscle actin and airway mucus in the airway remodeling and asthma related gene research literature summary. [Results] Improve airway inflammation astragalus polysaccharides from activation and inhibition EOS EOS infiltration reduction, regulating Th1/Th2 imbalance, so Th0 cells to Th1 cell differentiation, and inhibiting the release of inflammatory cytokines and other aspects, APS can reduce airway hyper-responsiveness mitigate aspects peribronchial infiltration of eosinophils and airway smooth muscle cells induced byα-SMA expression and other high alleviate airway remodeling. [Conclusion] The influence of astragalus polysaccharide on asthma gene research will lay the basis for study of the mechanism of treatment of asthma, monomer research provides new ideas for traditional Chinese medicine.

Past decade,plants as production systems for recombinant proteins has been actively investigated,and is one of the most active research fields in life sciences. Plants are attractive as protein factories because they with advantages of cost,scale,and safety,and emerge capability to produce pharmaceutical production with bioactivity proved by previously studies. The plant-made pharmaceuticals possess enormous viability for market and wide prospects for application. Based on those reasons,the rise of plant bioreactor was reviewed,and research advance on the expression systems and plant-made recombinant proteins was introduced. Bottleneck problems and adopted strategies using plant system to produce recombinant proteins and research hotspot in the near future were summarized,and prospect of plant bioreactor in future was described. Synchronously,some opinions including research progress of plant bioreactor,differences to foreign and strategies in the future were discussed.

[Objective] To investigate the effect of Suiqing Pill (SP), a Chinese prescription with the actions of activating blood and removing blood-stasis, on growth and metastasis of tumor. [Methods] Sixty C57BL/6 mice were randomized to three groups: group A (model control), group B (low-dose SP) and group C (high-dose SP). H22 hepatocarcinomatous metastasis models were established by subcutaneous injection of H22 hepatocarcinoma cells into the right lower limb. The inhibitory rate of tumor weight, incidence of metastatic carcinoma of lung, metastasis of lymph node, survival time and life-span prolongation rate were observed. [Results] The weight of tumor was reduced and the inhibitory rate of tumor weight was 36.83% in group B (P

Objective To study the effect of whole-process nursing intervention on comfort of patients undergoing radial artery puncture. Methods 100 patients with radial artery puncture from July, 2007 to June, 2008 were divided into the control group and the experimental group with 50 cases in each group accord-ing to time sequence. Routine whole nursing mode was used in the control group, the whole-process nursing intervention mode based upon routine nursing mode under the instruction of evidence-based method was used in the experimental group. Pain, psychological tensity, body numbness, success rate of radial artery puncture, patients' satisfaction degree were compared between the two groups. Results Every indexes of the experi-mental group were better than those of the control group. Conclusions The whole-process nursing interven-tion mode can promote the comfort degree of patients undergoing radial artery puncture.

Objective To evaluate the benefits and potential adverse effects of Cordyceps sinensis as an adjuvant immunosuppressive treatment in acute kidney injury.Methods English language database ( including MEDLINE, EMBASE and CENTRAL) in systematic review searched, using search terms relevant to this review.Chinese language databases ( including CNKI, CBM, WanFang and other resources) in systematic review also searched.It included all randomised controlled trials ( RCTs) and quasi-RCTs evaluating the benefits and potential side effects of Cordyceps sinensis for acute kidney injury, irrespective of blinding or publication language.An inclusion criterion was that immunosuppressive therapy baseline must be the same across arms in each study.Data were collected and analyzed by RevMan 5.0.Methodological risk of bias was assessed using the Cochrane risk of bias tool. Results This review included 7 RCTs (representing a total of 601 patients with acute kidney injury) that assessed the use of Cordyceps.Reporting of methods was incomplete in most studies.It conducted comparisons of Cordyceps versus no treatments on the base of same conventional treatment in both groups.Cordyceps for patients showed a favorable trends toward improving patient survival and reducing renal replace treatment, but the difference did not reach significance.However, Cordyceps significantly reduce the level of serum creatinine shorten the period of oliguria, and increase the osmotic pressure of urine.Conclusion On the base of same conventional treatment in both groups, compared with no treatment, Cordyceps significantly improves the functions of renal tubule and glomerulus.But long-term studies are needed to clarify any benefit-harm trade-off.Future studies also need to be appropriately sized and powered.

Objective To study the expression of caspase-3,survivin,CyclinD1 and P27 in gastric carcinoma(CA2)and the relationship between the biomarkers and clinical pathological parameters. Methods Immunohistochemical Ready-to-use two-steps method was performed to research the expression of the four proteins in 72 cases of GC,and 54 cases of normal gastric mucosa.Results The expression level of caspase- 3 and p27 protein in GC was significantly lower than that in normal gastric mucosa(P

Objective:This paper studied the electromagnetic-driven pressure control system in the automatic measurement of intraocular pressure (IOP).The accuracy of pressure control directly impacts on the accuracy of IOP measurements,so it calls for precise pressure to achieve closed-loop control.Methods:According to the actual needs of IOP measuring,we got the structural and electromagnetic parameters of the electromagnetic drive system by combining the methods of numerical calculation and ANSYS simulation.Using the FC bus and digital-analog conversion,the microcontroller module controlled the drive current digitally and precisely in order to achieve closed-loop control of pressure.Results:The experimental results showed that in the electromagnetic pressure control system,the pressure range was 0 N～0.48982 N,the accuracy 0.00058 N,the drive current control error about 1% on average.Besides,the electromagnetic force and the excitation current showed a good linear relationship in the same stroke.Conclusions:The electromagnetic pressure control system achieved a good result in closed-loop control of pressure.The control pressure range and control accuracy were in line with the standard of Goldmann applanation tonometer.According to the results,this study laid the foundation for the further development of IOP automatically measuring instruments.

AIM: The aim of the present study is to investigate changes of five biochemical markers of bone turnover during the formation of ovariectomized rat model. METHODS: Three-month-old female SD rats were divided randomly into ovariectomized (OVX) group, sham-operated (sham) group and control group. Five biochemical markers of bone turnover levels, including serum osteocalcin (OC), total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRAP) and hydroxyproline (HYP) were measured before and at 1, 1.5, 2, 2.5, 3 and 4 months after surgery. The right proximal tibias of rats were excised at the same time point for histopathological observation. RESULTS: Serum OC, ALP, BALP, TRAP and HYP levels in OVX group were significantly higher than that in sham group. As regard to the time of peak level arrived, the sequence of changes was as follows: TRAP/HYP→OC→ALP/BALP. There was a significant positive correlation between five markers. The pathological changes of trabecular bone in OVX group were only observed 3 months after surgery. CONCLUSION: The results suggest that postmenopausal osteoporosis has a high bone turnover rate. During the formation of ovariectomized rat model, changes of bone resorption markers precede changes of bone formation markers. Serum OC, ALP, BALP, TRAP and HYP are sensitive to evaluate the bone loss in the earlier stage of postmenopausal osteoporosis.

Objective To analyze the investigation results of an acute respiratory infection outbreak caused by adenovirus and provide scientific information for the prevention and control of congener public health emergencies .Methods A case‐control study was performed with grades and gender as matching factor ,all cases and selected controls were investigated with the same question‐naire .Results A totul of 47 cases were diagnosed in the outbreak ,no death ,the attack rate was 8 .88% ;the main clinical symptom was fever and 27 .7% of the cases became pneumonia .The case‐control study analysis demonstrated that with close contact to cases or not(χ2 =7 .96 ,P<0 .05) ,contact time (χ2 =7 .95 ,P<0 .05) ,hand washing habits (χ2 =25 .92 ,P<0 .05) and with or without the habit of cleaning snivel by hand directly (χ2 =22 .78 ,P<0 .05) were statistically different between cases and controls .Conclu‐sion long‐time contact to cases maybe the main risk factor for the adenovirus infection ,especially the contact manner were sharing the same desk or playing together .A good health habit of washing hands often and no cleaning snivel by hand directly were impor‐tant protective factors .Thus ,strengthening the training of health habit and awareness is the important preventive measure for re‐spiratory infectious diseases .

Objective To investigate the pathogen distribution and susceptibility profile of fungal isolates from bloodstream infections,and valuate the clinical utility of G test in diagnosis of fungal infections for the purpose to improve antifungal therapy.Methods A retrospective analysis was carried out to analyze the fungal pathogens isolated from bloodstream infections in the First Affiliated Hospital of Nanjing Medical University during the period from January 2013 through December 2015 and their antimicrobial susceptibility.Results A total of 114 fungal strains were isolated from bloodstream infections during the 3-year period,most of which were Candida (99/114,86.8％),especially Candida albicans (30.7％).About 41.2％ (47/114) of the fungal strains were isolated from Department of Thoracic Surgery (10,5 and 4 strains in 2013,2014 and 2015),Hematology (11 strains in 2014),and ICU (7 strains in 2014).Antimicrobial susceptibility testing showed that all the fungal strains (100％) were susceptible to amphotericin B,but 83.5％ susceptible to itraconazole (the lowest).G test was positive before the result of blood culture in 13 of the 54 patients who received G test.Conclusions Candida was the most common fungus in fungal bloodstream infection.Amphotericin B is the most active antifungal agent in vitro.Blood culture combined with serological test can provide clinicians an earlier and reliable diagnosis.