1.Relationship between Wnt/?-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2
Journal of Chongqing Medical University 2003;0(06):-
Objective:To study the regulatory mechanism of Wnt/?-Catenin passthway-influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of ?-Catenin mRNA,Bcl-2mRNA,Caspase-8mRNA and Caspase-3mRNA.Methods:(1) Expressions of ?-Catenin,Bcl-2,Caspase-8 and Caspase-3mRNAs in groups with or without indomethacin induction were detected by RT-PCR.(2) Methyl thiazolyl tetrazolium(MTT) assay was used to measure the anticancer activity of indomethacin.Results:(1)As compared with groups without indomethacin induction,expression of ?-Catenin mRNA and Bcl-2 mRNA were lower,however,increased expression of Caspase-8 and Caspase-3mRNA were found in SGC-7901 cells after treatment with indomethacin.(2) Indomethacin of 50 ?mol/L at 24 h couldn't induce apoptosis of gastric cancer cells.Indomethacin of 50?mol/L at 48 and 72 h and of 100?mol/L and 200?mol/L at 24、48 and 72 h could induce apoptosis of gastric cancer cells in a dose-and time-dependent manner.Conclusion:Gastric cancer cells may be caused by apoptosis inbalance through Wnt/?-Catenin passthway,which activates expression of proto-oncogene Bcl-2 and inhibites expressions of Caspase-8 and Caspase-3.
2.Tuina Treatment for 37 Cases of Postoperative Urin ary Retention
Journal of Acupuncture and Tuina Science 2009;7(2):116-117
Objective:To observe the effect of postoperative urinary retention treated with tuina therapy.Methods:Tuina therapy was applied on 37 cases of postoperative urinary retention;Qihai(CV 6),Guanyuan(CV 4),Zhongji(CV 3)and bilateral Sanyinjiao(SP 6)were selected.Results:Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments.Conclusion:Point tuina is an effective method in treating postoperative urinary retention.
3.Exploration and practice on experimental teaching in preventive medicine specialty
Chinese Journal of Medical Education Research 2011;10(3):306-308
By reviewing experiment teaching,the primal problems and solutions of practical training in preventive medicine specialty are explored in order to provide some beneficial reference and help for experimental teaching.
4.Research progress on mitochondrial oxidative stress and corresponding targeted delivery systems in Alzheimer's disease
Ling-ling ZHOU ; Kang QIAN ; Peng YANG ; Qi-zhi ZHANG
Acta Pharmaceutica Sinica 2022;57(6):1630-1640
Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of
5.Impact of Conditioned Medium of Decidual Stromal Cell Cluture on Proliferation,Apoptosis and Invasion of Trophoblast Cells
Ling KANG ; Guowu YIN ; Xiaoming ZHU
Journal of Practical Obstetrics and Gynecology 2017;33(6):420-425
Objective:To investigate the impact of conditioned medium of decidual stromal cell culture(DSCM) on proliferation,apoptosis and invasion of trophoblast cells.Methods:lsolution,culturing and indentifying the purity of decidual stromal cells from healthy women of early pregnancy to prepare different concentrations of DSCM (5%,20%,50% and 100%);Proliferation was evaluated by MTT assay;Cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining;Cell invasion was assayed using a Transwell chamber;RT-PCR and gelatin zymography were used to explore the mechanism of cell invasion.Results:Using this method we obtained a purity over 90% of decidual stromal cells.With the concentration of DSCM increased(5%,20%,50%,100%),the OD value of the MTT assay has elevated and the rate of apoptosis declined,the infiltrating cell increased as well (P < 0.05);Compared with control group,DSCM treatment led to a significant increase in the mRNA and protein expression of MMP-2/9(P <0.05).Conclusions:Decidual microenviroment may promote trophoblast proliferation and inhibite its apoptosis besides could enhance its ability of invasion by altering the expression of MMP-2 and MMP-9.
6.Left ventricular multiple myxomas in a neonate.
Chinese Journal of Pediatrics 2005;43(8):630-630
7.Analysis of Static Balance Function in Patients with Lumbar Disc Herniation
Kang LING ; Qing XIA ; Xiaoguang CAO ; Hai YUAN
Chinese Journal of Rehabilitation Theory and Practice 2016;22(6):715-718
Objective To investigate the static postural balance of patients with lumbar disc herniation (LDH). Methods From January to September, 2015, thirty patients with LDH were as observation group, and thirty healthy adult people were as control group. Their bal-ance function were detected and compared. Results The sway length, sway area and anteroposterior sway velocity were higher in the obser-vation group than in the control group (t>2.262, P<0.05), but there was no significant difference in mediolateral sway velocity between two groups (t=1.946, P=0.057) in eye-open condition. All of the indexes were higher in eye-closed condition in the observation group than in the control group (t>2.767, P<0.01), as well as the Romberg values (t>2.326, P<0.05). Conclusion Impairment of the proprioception and lower back pain affected the postural control of patients with LDH, who relied more on vision input to maintain postural stability.
8.Therapeutic and protective effects of high-density lipoprotein of human plasma on endotoxemia in rats
Ying HUANG ; Ling GU ; Shuren WANG ; Yan KANG ; Shuhong WU
Chinese Journal of Pathophysiology 2000;0(10):-
0.05). CONCLUSION:HDL of human plasma could attenuate or inhibit the decrease in BP induced by endotoxin and prolong the survival time.These results indicated that HDL has therapeutic and protective effect on rat with endotoxemia.Inhibition of TNF release might be one of mechanisms.
9.Calcineurin mediates the NF-?B p65 expression and neurotoxic ity induced by interleukin-1?
Ling GAO ; Jinsong KANG ; Zhongchi HOU ; Hui PAN ; Shigong ZHU
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To explore the role of calcineurin i n th e expression of NF-?B and the neurotoxicity in cultured cortical neurons treate d with interleukin-1? (IL-1?) and NMDA. METHODS: The cultured rat cortical neurons were used in the expe riment, damage of neurons was induced by interleukin-1?(IL-1?) or excitator y amino acid (NMDA). The degree of neuron damage was examined with the methods o f MTT assay and LDH releasing rate assay, as well as the Annexin V and PI immuno fluorescence. The expression of NF-?B p65 on the neurons was tested by the West ern blot analysis. RESULTS: Viability of neurons was obviously lower in the IL-1? group and NMDA group respectively than that in control group (P0.05). Annexin V and PI immunofluoresc ence showed that IL-1? mainly induced the neuron apoptosis, and NMDA induced th e neuron necrosis. CONCLUSIONS: The calcineurin mediates the higher expression of N F -?B p65 and neuron damage induced by IL-1?, but not play a critical role in th e necrosis induced by NMDA in the cultured cortical neurons. These results indic ate that calcineurin is the key molecule in the apoptotic signaling pathway.
10.New-type portable firstaid kit for evacuation corridor
Jianing LI ; Ting ZHANG ; Ling ZHANG ; Jun KANG
Chinese Medical Equipment Journal 1993;0(06):-
The paper mainly introuduces a kind of advanced portable firstaid kit,which is used in evacuation corridor by doctors,hygeians and nurses.It has such fuctions as hemostasis,bandaging,fixing,ventilation-improved respiration and contershock.With reasonable internal structure,it is very convenient to be quickly opened,closed and supplied.With the optimized allocation of ambulance drugs and materials,it meets the effective and modularization needs of field medical equipment.