L1 cell adhesion molecular is a type Ⅰ membrane glycoprotein of immunoglobulin superfamily. L1 is expressed mainly in nervous system and plays important roles in nervous system development, learning and memory. L1 cytoplasmic domain (L1CD) is important for L1 function by mediating signaling. To investigate the molecular mechanisms of signaling mediated by L1CD, yeast two-hybrid assay was carried out to screen the human brain cDNA library using L1CD as the bait. After sequence analysis and BLAST, several candidates were identified. One candidate is PAX6 transcription factor. L1CD and PAX6 cDNA were cloned in expression vectors and cotransfected into COS-7 cells. The interaction between L1CD and PAX6 was verified by co-immunoprecipitation assay. These preliminary results suggest that L1CD may be involved in transcription regulation.

The concentrations of CuZn-SOD and malondialdehyde (MDA) in in-flowingand out-going pulmonary blood(IPB, OPB) were observed dynamically during intestinal ischemic-reperfusion in rabbits. The results showed that in normal subjects the content of SOD of OPB was higher than IPB, P

Objective To evaluate CT diagnostic value of blunt spenic trauma.Methods 36 cases with blunt spenic trauma underwent CT scan,their CT features were analysed restrospectively.Results Of 36 cases,there were 28 cases of true spenic rupture,5 cases of central spenic rupture,3 cases of subcapsular rupture.CT manifestations were heterogenceous hematomas,laceration of spleen,perisplenic hematoma,subcapsular hematoma and hemoperitoneum.CT also discovered other injuries including those of liver,stomach,duodenum,lumbar spine and ribs,31 patients were underwent an operation for the spenic injuries.Only 5 were treated conservatively.Conclusion CT can accurately display extent and degree of the blunt spenic trauma,and also guide the clinical management.

OBJECTIVE: To discuss marketing combination program strategy of online drug store in China.METHODS: It was analyzed from aspects of product strategy,price strategy,channel strategy and promotion strategy.RESULTS & CONCLUSIONS: For product strategy of online drug store,great importance should be attached to the rational selection of products;the product strategy should manifest its advantage of low price;the distribution channel strategy should be conducive to the close rate and the promotion means in promoting strategy should be diversified.

OBJECTIVE:To provide reference for the selection of marketing strategy for post-marketing drugs.METHODS:?2 test was employed to analyze the number of 4 marketing strategies(flank attack strategy,guerrilla strategy,defensive strategy and attack strategy)used for the 100 well-known drug brands(74 from domestic versus 26 from abroad),meanwhile the regular marketing strategies for the domestic vs.foreign drug enterprises and for the OTC drugs vs.prescribed drugs were analyzed.RESULTS & CONCLUSIONS:There were significant differences between domestic and foreign pharmaceutical enterprises in the use of guerrilla strategy and attack strategy,20 of the domestic brands(27.03%)vs.none of the foreign brands used guerrilla strategy(0.00%),however,the foreign enterprises prefer attack strategy than domestic enterprises(14 foreign brands(53.85%)vs.13 domestic ones(17.57%)).There were no significant differences between domestic and foreign brands in the application of flank attack strategy and defensive strategy,the same is true between OTC and prescription drugs in the four strategies mentioned above.

OBJECTIVE:To provide references for domestic pharmaceutical enterprises in making marketing-mix strategy. METHODS: ?2 test was performed to analyze the numbers of the product strategy,price strategy,place strategy and promotion strategy included in marketing-mix strategy of 100 well-known brand drugs (74 domestic brand drugs versus 26 foreign brand drugs),and the regular patterns for the application of the strategies were summarized. RESULTS & CONCLUSIONS: There is great difference between domestic brand drugs and foreign brand drugs in product strategy and price strategy. In terms of product strategy,the foreign drug enterprises attach more importance to concept marketing strategy than domestic counterparts do;as for price strategy,the domestic drug enterprises prefer low price-high promotion strategy while the foreign counterparts prefer high price-high promotion strategy. However,the difference between Chinese drug enterprises and foreign counterparts in channel strategy and promotion strategy is non-significant.

Objective To determine the effect of prone position (PP) ventilation on oxygenation in patients with acute respiratory distress syndrome (ARDS) .Methods Twenty-three patients of both sexes (14 males, 9 females) with ARDS (within 72 h after onset of ARDS) were included in this study. The patients ranged in age from 43-78 years. Ten minutes before the patients were placed in prone position, midazolam 2-5 mg was given i. v. followed by continuous infusion at 2-3 mg?h-1 for sedation and a bolus of vecuronium 4 mg per hour for muscle relaxation. The patients were mechanically ventilated (VT 6-8 ml?kg-1, inspiration-time 1.0-1.2 s, RR 12-20 bpm, FiO2 0.4-1.0, PEEP 6-18 cm H2O) . The patients were placed in prone position for 2 h. Radial artery was cannulated and S-G catheter placed. MAP, CVP, MPAP, PAWP, cardiac output, PaO2 , PaCO2 peak inspiratory pressure (PIP) and airway resistance (Raw) were measured and recorded immediately before (T0) and 0.5, 2 h after the patients were placed in prone position (T1 , T2) and 2 h after supine position was resumed(T3) . PaO2/FiO2 and static compliance of the respiratory system (Cst) were calculated. Results In 87% of the patients PaO2/FiO2 was significantly increased during prone position (T1, T2) as compared to the baseline (T0) (P

The factors influencing the integration of MOOC and information literacy education in academic libraries were divided into objective factor, subjective factor and social factor according to their connotation and significance. Certain measures for their integration were proposed, such as strengthening network construction, perfecting literacy education systems, and carrying out systemic training.Integration of MOOC and information literacy education in academic libraries can improve information service, promote wide application of MOOC and information literacy ed-ucation in academic libraries.

In numerous digestive reconstruction techniques after proximal gastrectomy for tumor of the gastroesophageal junction,widely used methods are esophagogastrostomy,esophagogastric tube reconstruction and jejunalinterposition reconstruction.More studies have been focused on jejunalinterposition reconstruction in recent years,from which a variety of modified reconstructions derive.In clinical practice,a flexible choice is needed according to the actual situation of patients.

Objective To investigate the different gender and untreated hyperthyroidism parameter difference (hyperthyroidism) and hemoglobin of red blood cells in peripheral blood of patients with relatively healthy people.Methods Collecting blood samples of patients with hyperthyroidism,with EDTA-K2 anticoagulant,using Sysmex XE-2100 automated hematology analyzer analysis of peripheral blood hemoglobin in red blood cell parameters and using healthy people as healthy control.Results Healthy control group,male,female red blood cells and hemoglobin in patients with hyperthyroidism were increased,the difference was statistical-ly significant (P <0.05).Conclusion Newly diagnosed hyperthyroidism value and erythrocyte hemoglobin values higher than nor-mal.