Objective To investigate the impacts of Xuebijing injection on systemic inflammatory response and immune function of patients with acute exacerbation of severe chronic obstructive pulmonary disease (AECOPD). Methods A prospective randomized controlled trial (RCT) was conducted. Forty patients with severe AECOPD were divided into control group and Xuebijing group in accordance with the random number table, each group 20 cases. Both groups were treated by routine conventional basic therapy of severe AECOPD including anti-infection, phlegm-expelling formula, bronchodilators and mechanical ventilation, etc, while in Xuebijing group, intravenous drip of Xuebijing (100 mg, twice a day for 5 days) was additionally used. The changes of data of arterial blood gas analysis, blood routine examination, C-reactive protein (CRP), procalcitonin (PCT), immune function, and acute physiology and chronic health evaluation system Ⅱ (APACHE Ⅱ) score before and after therapy were observed and compared between the two groups. The length of stay in hospital, duration of mechanical ventilation, and prognosis were also compared between the two groups. Besides, according to the difference in APACHE Ⅱ score, all the patients were divided into APACHEⅡscore≥15 score group (18 cases) and APACHEⅡscore<15 score group (22 cases), and the immune function were compared between the two groups. Results ①The immune function was descended and disordered in patients with severe AECOPD. Compared with the < 15 score group, the expressions of CD45+, CD3+, CD4+, CD4+/CD8+ ratio, B-lymphocyte, natural killer cell (NK cell) in ≥ 15 score group were significantly lowered [CD45+(×106/L):663.92±100.61 vs. 1 289.92±169.38, CD3+(×106/L):342.05±108.93 vs. 882.37±172.56, CD4+(×106/L):205.96±63.97 vs. 486.24±108.64, CD4+/CD8+ratio:0.76±0.49 vs. 1.32±0.57, B-lymphocyte (×106/L):124.77±32.72 vs. 166.06±48.02, NK cells (×106/L): 186.47±39.57 vs. 243.51±44.72, all P < 0.05]. There was no statistically significant difference in the expression of CD8+ between the ≥ 15 score group and < 15 score group (P > 0.05). ② Compared with those before therapy, the pH value, oxygenation index, arterial partial pressure of carbon dioxide (PaCO2), white blood cell count (WBC), CRP, PCT were significantly improved after therapy in both control and Xuebijing groups. Compared with those in the control group, WBC, CRP, PCT were significantly lowered in Xuebijing group [WBC (×109/L): 10.29±3.83 vs. 12.69±3.42, CRP (mg/L): 9.06±4.19 vs. 15.26±4.22, PCT (ng/L): 0.18±0.21 vs. 0.42±0.24, all P < 0.05]. There were no statistically significant differences in pH value, oxygenation index and PaCO2 between two groups (all P>0.05). The degrees of improvement of CD45+, CD3+, CD4+, CD8+ and CD4+/CD8+ ratio were more remarkable in Xuebijing group after treatment than those in control group [CD45+ (×106/L): 1 079.38±153.86 vs. 1 015.63±157.11, CD3+ (×106/L): 652.05±100.05 vs. 596.81±106.85, CD4+ (×106/L): 358.92±67.53 vs. 329.99±72.61, CD8+ (×106/L): 205.73±35.19 vs. 230.41±39.74, CD4+/CD8+ratio: 2.16±0.63 vs. 1.52±0.54, all P < 0.05]. The B-lymphocyte and NK cell before treatment and after treatment showed no statistical significant differences between the two groups (all P > 0.05). Compared with the control group, the duration of mechanical ventilation (hours: 56.25±22.87 vs. 69.45±26.59) and the length of stay in hospital (days: 11.00±0.74 vs. 14.00±2.06) were shortened, and APACHE Ⅱ score was significantly lowered in Xuebijing group (8.21±2.97 vs. 12.08±3.12, P < 0.05). The numbers of multiple organ failure and dead patients in Xuebijing group were less than those of control group, but no statistical significant differences were found (both P > 0.05). Conclusion Xuebijing injection for treatment of patients with severe AECOPD can ameliorate inflammatory response, improve immune function, shorten the duration of mechanical ventilation and the length of stay in hospital, and decrease the risk of death.

Objective To understand the clinical distribution and drug resistance situation of112 strains of Acinetobacter Bau-mannii isolated from the patients′sputum samples in cerebral surgery ICU in order to provide the basis for the rational use of anti-bacterial drugs in clinical therapy.Methods 112 strains of Acinetobacter Baumannii were performed the retrospective analysis.The VITEK2-Compact automatic microbiological analyzer was adopted to conduct the bacterial identification and the drug susceptibility test and the K-B method was combined as the supplement control.The Whonet 5.6 software was used for conducting the statistical analysis.Results Among 112 strains of Acinetobacter baumannii,75 strains(67.0%)were multi-drug resistant,8 strains(7.1%) were pan-resistant.The antimicrobial susceptibility tests showed that the resistant rates of Acinetobacter baumannii to mezlocillin, piperacillin and nitrofurantoin reached up to 100%.Acinetobacter Baumannii had the lower resistant rate to polymyxin B (2.7%), the resistant rates to minomycin and ceperazone/sulbactam were 4.5% and 10.7% respectively.Conclusion The drug resistance phenomena of Acinetobacter Baumannii in the cerebral surgery department ICU is serious.Minomycin,polymyxin B and ceperazone/sulbactam have the better antibacterial activities to clinically isolated Acinetobacter Baumannii and may be the alternative drugs for therapy.Clinic should strengthen the monitoring and rationally use the antibacterial drugs.

Heart Defects, Congenital ; therapy ; Humans

Culture ; Medicine, Chinese Traditional ; Research ; Translations

Arrhythmias, Cardiac ; therapy ; Biomedical Research ; methods ; standards ; Catheter Ablation ; Child ; China ; Drug Therapy ; Humans

Adult ; Burns ; etiology ; Humans ; Male ; Occupational Exposure ; Phosphorus ; adverse effects ; Young Adult

Cardiac Catheterization ; trends ; Forecasting ; Heart Defects, Congenital ; therapy ; Humans

The paper introduces reference work in the new age from its modes, contents, characteristics and developing strategies,including establishing characteristic resources, improving quality of librarians, carrying out network consultant and information navigation, establishing collaborative digital reference, in order to provide reference for primary hospital libraries.

Background The cytochrome P450-dependent monooxygenase 2D6 (CYP2D6) ac- tivity can he estimated by investigating the metabolism of model drugs or by genotyping the most com- mon CYP2D6 alleles. The CYP2D6 allele frequencies are well investigated in Caucasians whereas no comprehensive allele analysis has been done in mainland Chinese. The aim of the study was to investi- gate C2938T, G1846T, G1846A and C188T gene mutation in mainland Chinese. Methods Three tet- ra-primer PCR assays and one allele specific amplification (ASA)-PCR assay were developed to easily genotype C2938T, G1846T, G1846A and C188T mutation in CYP2D6 allele in 223 cases of mainland Chinese. Results The most frequent allele was * 10 (C188T) with a prevalence of 51.3%, followed by * 2 allele (C2938, 15. 5%) in mainland Chinese. The distribution frequence of a novel variant CYP2D6 * 14B was 2.0%. This novel * 14B allele was different from * 14 allele by the absence of the C188T substitution and by the additional G1749C substitution. Conclusion This comprehensive study of CYP2D6 allele in mainland Chinese shows some genetic diversity compared to Chinese from other regions. Genotyping is a useful method in applying drug individuation.

Objective To evaluate the levels of plasma N-terminal pro-brain natriuretic peptide (NT-proBNP),high sensitive C-reactive protein (hs-CRP) and the correlation in the patients with actue coronary syndrome (ACS).Methods One hundred and eight patients with ACS as ACS group,while 84 healthy controls as control group.ACS group included non-ST-segment elevation myocardial infarction (NSTEMI) group (37 cases),ST-segment elevation myocardial infarction (STEMI) group (35 cases),unstable angina(UA) group(36 cases).Recorded and compared the levels of NT-proBNP and hs-CRP in two groups,and the correlation was analyzed.Results The levels of NT-proBNP and hs-CRP in ACS group were significantly higher than those in control group [(1264.8 ±671.8) ng/L vs.(337.0 ± 10.6) ng/L,(15.42 ± 8.03) mg/L vs.(6.92 ± 0.59) mg/L,P ＜ 0.05].The correlation between hs-CRP and NT-proBNP level was positive in ACS (r =0.46,P ＜ 0.05).The levels of NT-proBNP in NSTEMI group,STEMI group,UA group,control group,differences among groups were statistically significant (P ＜ 0.05).The levels of hs-CRP in NSTEMI group,STEMI group,UA group were significantly higher than those in control group (P ＜ 0.05).There was no statistically significant difference in the levels of NT-proBNP between STEMI group and UA group (P ＞0.05).And the levels of NT-proBNP and hs-CRP increased with the increasing of coronary artery lesion counts,the differences among groups were statistically significant (P ＜0.05).Conclusion ACS can be early diagnosed through combined detection of NT-proBNP and hs-CRP,and NT-proBNP and hs-CRP can be used as important makers to evaluate prognosis and risk stratification.