2.Expression of COX-2 and the relationship between COX-2 and PTEN in endometrial adenocarcinoma
Huan-Hua WANG ; Yi-Lin WU ; Zhi-Sheng ZHANG ; Li-Ling WU ;
Cancer Research and Clinic 2006;0(08):-
Objective To study the expression and clinical significance of COX-2 and the relation- ship between COX-2 and PTEN in endometrial adenocarcinoma(EAC).Methods The expression of COX-2, PTEN protein was detected by SP-immunohistochemical method in EAC,endometrial intraepithelial neoplasia, corresponding normal endometrium.Results The positive rates of COX-2 protein increased from normal en- dometrium(13.33%) to endometrial intraepithelial neoplasia(50.00%) and adenocarcinoma(64.58 %)(P
3.Recent advances and perspective in the study of the nano-reinforcing materials for molecular imprinting of proteins.
Zhi-hui WU ; Miao-ling CHAI ; Jia-peng HOU ; Jun PAN
Acta Pharmaceutica Sinica 2015;50(1):15-20
Molecular imprinting technique (MIT) involves the synthesis of polymer in the presence of a template to produce complementary binding sites in terms of its size, shape and functional group orientation. Such kind of polymer possesses specific recognition ability towards its template molecule. Despite the rapid development of MIT over the years, the majority of the template molecules that have been studied are small molecules, while molecular imprinting of proteins remains a significant yet challenging task due to their large size, structural flexibility and complex conformation. This review, we summarized the research findings over the past years, and discussed the nano-reinforcing materials used to prepare molecular imprinting of proteins and the perspective of these nano-reinforcing materials.
Binding Sites
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Molecular Conformation
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Molecular Imprinting
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Nanostructures
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chemistry
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Polymers
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chemistry
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Proteins
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chemistry
4.Hypoxia-responsive factor PHD2 and angiogenic diseases.
Hui-Zhen JIA ; Vivi KASIM ; Zhi-Ling XU ; Li YANG ; Shou-Rong WU
Acta Pharmaceutica Sinica 2014;49(2):151-157
Prolyl-4-hydroxylase domain (PHDs) family is one of the most important regulatory factors in hypoxic stress. PHD2 plays a critical role in cells and tissues adaptation to the low oxygen environment. Its hydroxylation activity regulates the stability and transcriptional activity of the hypoxia-inducible factor 1 (HIF-1), which is the key factor in response to hypoxic stress. Subsequently, PHD2 acts as an important factor in oxygen homeostasis. Studies have shown that PHD2, through its regulation on HIF-1, plays an important role in the post-ischemic neovascularization. Furthermore, under hypoxic condition, PHD2 also regulates other pathways that positively regulate angiogenesis factors HIF-1 independently. Moreover, recently, several evidences have also shown that PHD2 also affects tumor growth and metastasis in a tumor microenvironment. Based on these facts, PHD2 have been considered as a potential therapeutic target both in treating ischemic diseases and tumors. Here, we review the molecular regulation mechanism of PHD2 and its physiological and pathological functions. We focus on the role of PHD2 in both therapeutic angiogenesis for ischemic disease and tumor angiogenesis, and the current progress in utilizing PHD2 as a therapeutic target.
Animals
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Humans
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Hydroxylation
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Hypoxia-Inducible Factor 1
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metabolism
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Hypoxia-Inducible Factor-Proline Dioxygenases
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antagonists & inhibitors
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physiology
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Neoplasms
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blood supply
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metabolism
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pathology
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therapy
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Neovascularization, Pathologic
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metabolism
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pathology
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Tumor Microenvironment
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Vascular Diseases
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pathology
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therapy
5.A Novel Screening Method for Isolating High Cholesterol Oxidase Producing Strain
Hui-Zhi HUO ; Ling ZHANG ; Yan SUN ; Hai-Lin YANG ; Wu WANG ;
Microbiology 1992;0(04):-
The cholesterol oxidase producing strain Brevibacterium sp.DGCDC-82 was treated with NTG (1 mg/mL)under ultrasonicztion(200 W,50 kHz).A red mutant named Brevibacterium sp.DGCCN-25 showed higher and stable production of cholesterol oxidase was obtained.The enzyme activity was increased by 140%,it is 1.24 U/mL.Then dealed with DGCCN-25 using the same method,two revertants were obtained,one was white and the other was rose pink.The enzyme activity of two revertants was obvious decrease,they are 0.17 U/mL and 0.69 U/mL.The results showed the positive correlation between COD acticity and red pigment producing by Brevibacterium sp..The relativity model can be used as a method of screening for mutation and directed evolution.
6.Effect of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on microvessel of rabbit VX2 liver tumor models
Juan KANG ; Xiao-ling WU ; Yong ZHANG ; Hai-tao RAN ; Zhi-gang WANG
Chinese Journal of Interventional Imaging and Therapy 2011;08(5):431-434
ObjectiveTo observe the inhibition effect of docetaxel-loaded lipid microbubbles (DLLM) combined with ultrasound targeted microbubbles destruction (UTMD) on microvessel in rabbit VX2 liver tumor models.MethodsSixty rabbits were randomly divided into 6 groups (n= 10),i.e.Doc group (used docetaxel only),DLLM group (used docetaxel-loaded lipid microbubbles),Doc+US group (used docetaxel combined with ultrasound positioning irradiation),PLM+US group (used microbubbles combined with ultrasound positioning irradiation),DLLM+US group (used docetaxel-loaded lipid microbubbles combined with ultrasound positioning irradiation) and control group.The expression of CD34 and VEGF and microvessel density (MVD) were compared among different groups.ResultsAfter treatment,the expression of CD34 in DLLM+US group was lower,the MVD of DLLM+US group was markedly lower than that of the other groups (P<0.01),while the expression of VEGF in this group was the lowest among all 6 groups (P< 0.01).ConclusionDLLM combined with UTMD can inhibit the generation of microvessels in rabbit VX2 liver tumor,thus inhibit the growth of the tumor.
7.Clinical analysis of 42 cases of primary malignant melanoma in female genital tract
Ju-Sheng AN ; Ling-Ying WU ; Ning LI ; Bin LI ; Gao-Zhi YU ; Li-Ying LIU ;
Chinese Journal of Obstetrics and Gynecology 2001;0(05):-
0.05). Conclusions Biopsy for the malignant melanoma in female genital tract has high misdiagnosis rate. Immunohistochemistry assay could improve diagnosis markedly.The FIGO staging system fails to predict the prognosis accurately.Surgery plays an important role in treatment,while the adjuvant chemotherapy could improve survival effectively.
8.Free fatty acids regulate expression of adiponectin receptor gene in adipocytes
Yu WEN ; Hong-Wei WANG ; Jing WU ; Hui-Ling LU ; Xiu-Fen HU ; Zhi XIA ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
3T3-LI preadipocytes were induced to differentiate and 3T3-L1 adipocyte or preadipocytes were incubated with oleate or palmitate overnight.RT-PCR was used to measure adiponectin receptor(AdipoR)1 and AdipoR2 mRNA levels.The results showed that the AdipoRl and AdipoR2 expressions were differentiation- dependent.Oleate only suppressed AdipoR mRNA expression in preadipocyte but not in adipocyte.However,high concentration of palmitate reduced AdipoR mRNA expression in both 3T3-LI preadipocyte and adipocyte.
9.Relationship between bone mineral density and polymorphism of vitamin D receptor gene in postmenopausal women in Guangzhou
Wen WU ; Ximei ZHI ; Dongfeng LI ; Kai LIN ; Ling XU ; Yanhong YANG
Chinese Journal of Pathophysiology 1986;0(03):-
0.05). CONCLUSION: Genotype of VDR is related to BMD, but there is no enough evidence to support genotype of VDR as a genetic marker in predicting the risk of developing osteoporosis in Guangzhou postmenopausal women.
10.Application of peak expiratory flow rate in asthmatic children
yan-ling, YUE ; xin, WU ; zhi-gang, ZHANG ; shu-zhen, YIN
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To study the clinical application of the peak expiratory flow rate( PEFR) in children with asthma.Methods The PEFRs of 43 cases of asthma at the acute stage were measured to guide the clinical grading and therapy by the optimum individual PEFR of the patients at the remission stage, and the descending rate and warning value of individual PEFR were determined to investigate the relationship between the individual PEFR descending rate and the asthma attack conditions in the standard and nonstandard monitoring groups.Results At the asthma attack stage, the clinical symptoms became severe with PEFR declining; at the remission stage, the preventive application of drugs was based on the changes of the individual PEEF descending rate . The case number and frequency of asthma attack and the cases subjected to moderate or severe attacks in the standard group were 15 %,23.5 % and 25 % respectively; while those in the nonstandard group were 43.5 %, 75.5 % and 76.9 % respectively, which showed a significant difference( P