Objective To observe the expression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in the hippocampi of rats stressed by restraint,and adjustments to 11β-HSD1 expression in response to electroacupuncture.The mechanisms of adjustment of the hypothalamic-pituitary-adrenal axis(HPA) to electroacupuncture were also studied.Methods Rats were randomly divided into a control group,a group stressed by restraint and an electroacupuncture group.The rats in the control group received no treatment. The rats in the strssd group were put into a small columnar cage and their hind legs tied outside the cage. The electroacupuncture group, in addition to being restrained,received electroacupuncture at the Zusanli acupoints on both sides of their bodies. The rats were then sacrificed and their hippocampi were isolated and lysed. The expression of 11β-HSD1 in each hippocampus were observed using the Western blotting technique. Results The leves of expression of 11β-HSD1 in the hippocampi of the restrained group were significantly higher than those in the control group.After electroacupuncture,11β-HSD1 expression in the hippocampus increased further and lasted 3 h. Conclusion Electroacupuncture at the Zusanli acupoints (ST36) can increase 11β-HSD1 expression in the hippocampus of stressed rats, and this adjustment may be related to the HPA axis' negative feedback function.

Breast Neoplasms ; diagnosis ; therapy ; Female ; Humans

Humans ; Rectal Neoplasms ; diagnosis ; therapy

Accidents, Occupational ; Acute Disease ; Adult ; Ethylene Oxide ; poisoning ; Female ; Humans ; Male ; Middle Aged ; Operating Rooms ; Young Adult

Air Pollutants, Occupational ; Humans ; Occupational Exposure ; Styrene

OBJECTIVE: To optimize the formulation of ambroxol hydrochloride sandwich osmotic pump tablets using Box-Behnken design response surface method. METHODS: Firstly, significant factors, including amount of glucose, ratio of pore former to weight of coating, and thicknesss of coating, were chosen carefully by single-factor methods, then Box-Behnken design-response surface method was adopted to understand the effects of these factors on the drug release by using similarity factor f2 as evaluation index. And multiple linear regression model and quadratic polynomial equation were established to evaluate the relationship between these factors and similarity factor f2. Secondly, the permeation mechanism of sandwich osmotic pump was studied carefully by SEM and drug release behavior investigation. RESULTS: The quadratic polynomial equation was ideal to fit the prediction result of drug release; the optimal formulation according to Design-Expert8.0.5 software was established as follows: 50 mg of glucose, 8% of pore former, 9% of coating weight. The f2 was approximate 89.33 compared with ideal drug release curve, which was relatively close to the model-predicted f2 value (88.89). Through investigation of permeation mechanism, it was found that the drug release from this delivery system was driven by osmotic pressure difference between the interior of tablets and the release medium. By means of SEM observation, it was shown that some pores on the coating surface were produced after drug release in the medium, but few before drug release, which cued that a lot of medium got into the interior of tablets through the tunnels formed by the porogen in the coating. CONCLUSION: Box-Behnken design method can be used to optimize the formulation of sandwich osmotic pump tablets.

A unilateral tinnitus occurred at right ear in a hypertensive and diabetic woman shortly after an ipsilateral peripheral facial palsy. Audiometric tests showed a predominant sensorineural hearing impairment at right ear. Her tinnitus was abolished after an administration of a low dose of gabapentin. In view of a controversy of gabapentin and tinnitus in previous trials, the fi ndings in this patient support that low dose gabapentin can benefi t specifi cally the subgroup of tinnitus patient with sensorineural impairment due to secondary contributing factor.

Purpose:To investigate the expression of E cadherin and nm23 in papillary thyroid carcinoma (PTC) and its clinical significance. Methods:The expression of E cadherin and nm23 were detected by SP immunohistochemical technique in 70 paraffin embedded tissue specimens of PTC.Results:The positive rates of E cadherin and nm23 were 44.3%(31/70) and 62.9%(44/70) respectively. There were close relations between the expression of E cadherin and nm23 and pathological grade, TNM stage and lymph node metastasis, and no difference could be found between the expression of E cadherin and nm23 and size of tumor, sex and age of patients. There was distinct correlation between the expression of E cadherin and nm23. The metastatic rate in the group, which the expression of E cadherin and nm23 were both positive, was 12%(3/25), but in the all negative group, the metastatic rate was 75%(15/20). There was significant difference between the two groups ( P

In order to investigate the effects of simvastatin on secretion and mRNA expression of interleukin-6 (IL-6) and adiponectin in 3T3-L1 adipocytes, mouse 3T3-L1 adipocytes were stimulated with lipopolysaccharide (LPS). Production and mRNA expression of IL-6 and adiponectin in 3T3-L1 adipocytes were measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. The results showed that simvastatin could significantly suppress LPS-induced IL-6 production and mRNA expression in adipocytes (P<0.05), but increase the LPS-induced adiponectin secretion and mRNA expression in a dose-dependent manner (P<0.05). It was suggested that simvastatin could exert beneficial effects on prevention of obesity-induced metabolic changes in adipocytes.

To study the application of simulated-patient teaching in practice teaching and assessment of obstetrics and gynecology,the two groups of students were taught by simulated-patient teaching and discussed cases teaching respectively,and then they were assessed by using simulated patients for clinical skills.The result shows that simulated-patient teaching is effective in improving students' clinical skills,can act as a worthy tool of clinical teaching and assessment.