1.Combined Toxic Effects of DBP and DEHP on Spermatogenesis in Male Rats
Xiaomei TIAN ; Ling LI ; Qiru SONG
Journal of Environment and Health 2007;0(10):-
0.05).DBP and DEHP obviously induced a decrease in organ body weight ratios of testis and epididymis and an increase in organ body weights ratios of liver.Obvious decrease in the sperm counts,spermatozoon survival rate and significant increase in the rate of the sperm deformation were observed.There was synergism between DBP and DEHP on the testis,epididymis and liver organ body weight ratio,as well as sperm counts,sperm survival and deformation rates.Pathological examination showed the seminiferous tubules were irregular shape,degenerative atrophy and interstitial substance broadening,and the seminiferous epithelium were degeneration.Epididymis epithelium was damaged and few of mature sperm was seen.Conclusion DBP combined with DEHP can cause obvious toxic effects on reproductive function in male rats.DBP and DEHP mixture can strongly affect the sperm quantity and quality.Also,some toxic effects to epididymis are observed.
2.Combined Toxic Effects of Di-n-butyl Phthalate and Di-2-ethylhexyl Phthalate on Fertility in Male Rats
Xiaomei TIAN ; Ling LI ; Qiru SONG
Journal of Environment and Health 1992;0(04):-
Objective To study the combined toxic effects of di-n-butyl phthalate(DBP)and di-2-ethylhexyl phthalate(DEHP)on sex hormone and lipid peroxidation in male rats.Methods According to 2?2 factorial analysis,thirty-two healthy and clean adult male SD rats were randomly divided into 4 groups,including a control group(given coin oil) and three experimental groups:DBP(1/20 LD50,1.0 g/kg,dissolved in coin oil),DEHP(1/20 LD50,1.7 g/kg,dissolved in coin oil) and DBP+DEHP(1.0 g/kg + 1.7 g/kg,dissolved in coin oil),8 rats in each group,through gavage,once a day,for 8 consecutive weeks.The spectrophotometric method was used to measure the activity of lipid peroxidation SOD,GSH and GSH-Px level in testis homogenate.The activities of ACP,AKP and ?-GT were assessed in testis homogenate.Radioimmunoassay was used to determine the testosterone,LH and FSH levels in the serum.Results There was synergism between DBP and DEHP on the SOD activity in testicle and testosterone level in serum,and there was antagonism between DBP and DEHP on the ?-GT and ACP activity in testicle,as well as the FSH level in serum.Conclusion DBP combined with DEHP can cause obvious toxic effects on reproductive function in male rats.The change of testosterone biosynthetic enzymes and the levels of T in serum and disordered physiologic balances of hypothalamic-pituitarytestis axis may be key factors contributing to the decrease of testosterone,then the decrease of reproductive function in male rats.
3.Effect of olmesartan medoxomil on renal oxidative stress in mice with chronic heart failure
Xiaomei LU ; Ling MA ; Yanqiu YU
Chinese Journal of Nephrology 2011;27(3):190-193
Objective To investigate the effect of olmesartan medoxomil on renal oxidative stress in mice with chronic heart failure. Methods C57 mice were divided into sham operation group(SHAM group),chronic heart failure group(CHF group)and olmesartan medoxomil treatment group(OLM group).Experimental CHF model was established by coronary artery ligation,in which OLM group fed with a daily dose of 10 mg/kg.The heart rate,blood pressure,cardiac function,Scr,BUN,and plasma and kidney angiotensin(Ang)Ⅱ were measured.Real-time PCR was used to examine renal gp91phox,p22phox and NOX4 expression.AZAN and DHE staining was used to detect renal pathological change after 12 weeks. Results Compared with SHAM group,left ventricular-end diastolic dimension (LVDd)and left ventricular end-systolic dimension(LVDs)were significantly increased(P<0.05),while fractional shortening(FS)and ejection fraction(EF)were significantly decreased in CHF and OLM groups (P<0.05).Compared with SHAM group,systolic blood pressure,Scr,BUN,and AZAN and DHE staining positive area were significantly increased in CHF group(P<0.05),while above indexes were significantly lower in OLM group as compared to CHF group(P<0.05).Compared with SHAM group,plasma and kidney Ang Ⅱ levels,gp91phox,p22phox and NOX4 expression were increased in CHF group(P<0.05),while above indexes were significantly lower in OLM group as compared to CHF group (P<0.05).Conclusions Chronic heart failure can activate intrarenal NADPH oxidase resulting in renal injury.Olmesartan medoxomil can protect kidney by inhibiting the effect of Ang Ⅱ-induced oxidative stress.
4.Expression of CD44s in lung cancer and its significance
Xiaomei WANG ; Zhiqiang CHENG ; Ling WANG ; Xuejin SU ; Zhuohuai CHEN
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To investigate expression of CD44s in lung cancer and it's clinical significance. METHODS: A total of 117 primary lung cancer from patients were examined for CD44s expression by immunohistochemical staining. RESULTS: CD44s mostly expressed in non-small cell lung cancer (NSCLC) but not in small ecll lung cancer (SCLC), and squamous cell carcinoma(SCC) showed much stronger expression of CD44s than adenocarcinoma(ADC)(P
5.Effects of atorvastatin on expressions of peroxisome proliferators-activated receptor gamma and MMP-9 in atherosclerosis rabbits
Xiaomei WANG ; Ling QIN ; Yang LI ; Kexin HUANG
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To study the effects of atorvastatin on the expressions of peroxisome proliferators-activated receptor gamma(PPAR?)and matrix metalloproteinase-9(MMP-9)in atherosclerosis(AS) tissue.Methods Atherosclerotic rabbit models on aortas were established with the high-cholesterol diet,and animals were divided into control,model and atorvastatin groups(n=8).The expressions of PPAR? and MMP-9 and the effects of atorvastatin on them were observed by means of immunohistochemistry.Results The expression of PPAR? in model group(14.38%?2.58%)was higher than that in control group(7.82%?0.96%)(P
6.Relationship of hypercholesterolemia with vascular endothelium function in rabbits
Ling QIN ; Dongmei CHEN ; Kexin HUANG ; Shaoyan LIU ; Xiaomei WANG
Basic & Clinical Medicine 2006;0(07):-
Objective To study the expression of nitricoxide(NO) and intercellular adhesion molecule-1(ICAM-1) in the animals with hypercholesterolemia and atherosclerosis(AS) in order to investigate the relationship of hypercholesterolemia with AS and vascular endothelium function and ICAM-1. Methods Fed rabbits with high-cholesterol diet to establish the models of hypercholesterolemia and AS,and the control group was fed with normal diet;Blood was collected from vein in 0,8 and 16 weeks in two groups,and the concentrations of TC,LDL,NO and ICAM-1 were measured;The correlation among TC,LDL and NO,ICAM-1 was observed;After 16 weeks,the rabbits were put to death,and immunohistochemistry analysis and RT-PCR were performed to measure the expression of ICAM-1 in the aortas.Results TC and LDL in 8 weeks were higher in the high-cholestrol diet group,but NO and ICAM-1 had no change;NO decreased and ICAM-1 increased in 16 weeks(P
7.Relationship Between the Levels of Plasma Calcitonin Gene Related Peptide and Cardiac Function in Ventricular Septal Defect Children Before and After the Interventional Therapy
Ling LIU ; Jun LIU ; Lei GAO ; Xiaomei HE ; Milin ZHANG ; Zhen WANG
Chinese Circulation Journal 2014;(11):921-923
Objective: To explore the relationship between plasma levels of calcitonin gene related peptide (CGRP) and cardiac function in ventricular septal defect (VSD) children before and after the interventional therapy.
Methods: Our research included 2 groups:VSD group, n=90 containing 43 male and 47 female at the age of (3-12) years and treated in our hospital from 2011-09 to 2012-09, Control group, n=50 healthy children with matched age and gender. The levels CGRP were measured at before and 24 hours, 3 days, 5 days, 3 months after the therapy. The cardiac function as left ventricle end-diastolic diameter (LVEDD), left ventricle end systolic diameter (LVESD), left ventricle end diastolic volume (LVEDV), left ventricle end systolic volume (LVESV) and ejection fraction (EF) were examined by echocardiography at before and after the procedure.
Results: Before interventional procedure, the level of CGRP in VSD group was lower than Control group (35.28 ± 7.25) pg/ml vs (50.90 ± 8.60) pg/ml, t=11.42, P<0.01. In VSD group, the post-procedural levels of CGRP were increased at all time points, the highest level was at 24 hours and the descending started at 3 days after procedure;the levels of CGRP at before and 24 hours, 3 days, 5 days after the procedure were different as (35.28 ± 7.25) pg/ml vs (102.03 ± 11.73) pg/ml, (35.28 ± 7.25) pg/ml vs (93.36 ± 9.12) pg/ml, (35.28 ± 7.25) pg/ml vs (87.91 ± 8.54) pg/ml, t=27.03, 26.93, 24.51, all P<0.01. In VSD group, the LVEDD, LVESD, LVEDV and LVESV decreased gradually at 5 days, 3 months and 6 months after the procedure;the levels of CGRP were signiifcantly related to LVEDD, LVESD, LVEDV, LVESV at before and 5 days, 3 months after the procedure, all P<0.05.
Conclusion: Plasma levels of CGRP closely related to ventricular cavity diameter, which was important for evaluating the cardiac function in VSD children after the interventional therapy.
8.Levels of 25-(OH)D in 0 ~ 12 years Old Children in Kunming
Yu LING ; Yanhui SUN ; Jihua CUI ; Jianmin WU ; Yu SONG ; Shujin LI ; Xiaomei LIU
Journal of Kunming Medical University 2016;37(12):34-36
Objective To learn the levels of serum 25-(OH)D in 0 12 years old chindren in Kunming.Methods Serum 25-(OH)D levels of children (4 498) were measured by using Roche cobas 8 000 / E602 automatic electrochemical luminescence immunoassay.Results As the increase of age,the levels of serum 25-(OH)D were gradually decreased,different age groups had significant difference (P<0.05).The levels of 25-(OH)D between boys and girls had no significant difference (P>0.05);The average level of sernm 25-(OH)D was 83.48 nmol/L,the level of serum 25-(OH)D was distributed in 0 ~ 1 years old group,and most concentrated in the 6 ~ 9 years old group.Conclusion The levels of 25-(OH)D in 0 ~ 12 years old chindren are in a good status,but with the increase of age,the level of 25-(OH)D is decreasing gradually,especially the levels of 25-(OH)D decreased significantly after the age of three,in shortage or lack of status,this should cause everybody's attention.
9.Synergistic effect of vorinostat and melphalan on human multiple myeloma cell lines
Wenjie XIONG ; Yan LING ; Chunrui FENG ; Xiaomei TAO ; Qiongli ZHANG ; Xin DU
Cancer Research and Clinic 2012;24(6):386-388
Objective To observe the anti-tumor effect on human multiple myeloma cell lines U266 and KM3 with a combination of varinostat and melphalan in vitro.Methods The cell proliferation of U266 and KM3 was datected with MTT assay when treated them with vorinostat alone and melphalan alone,then calculate their IC50 values respectively.Fixed the concentrations of vorinostator melphalan,the cell proliferation was datected in combination with melphalan/vorinostat in seriesly concentrations by MTr assay.Then to calculate drug combination index(CI).Results The IC50 value of U266 was 5.0-7.5 μmol/L and that of KM3 was 2.5-5.0 μmol/L when treated by vorinostat alone,the IC50 value of U266 was 40-60 μmol/L and that of KM3 was 60-80 μmol/L treated by melphalan alone.When fixed the concentration of vorinostat(in U266 the concentration was 1.25 μmol/L,in KM3 the concentration was 1.0 μ mol/L),Synergism(CI<0.9)was observed when the concentrations of melphalan were 20 μmol/L,40 μmol/L,60 μ mol/L and 80 μ mol/L in U266,40 μmol/L,60 μmol/L,80 μmol/L and 100 μmol/L in KM3;When fixed the concertration of melphalan (in U266,was 10 μmol/L,in KM3 was 20 μmol/L),synergism(CI<0.9)was observed when the concentrations of vorinostat were 1.0 μmol/L,2.5 μmol/L,5.0 μmol/L and 7.5 μmol/L in U266,and 1.0 μmol/L,2.5μmol/L,5.0 μmol/L in KM3.An additive effect was observed with the czombination of vorinostat 7.5 μmol/L plus melphalan 20 μmol/L in KM3(CI=0.93).Conclusion Vorinostat had potential anti-myeloma effect alone,and had synergistic anti-tumor effect with melphalan in vitro.
10.Detection of IgG2b Monoclonal Antibodies Against LDH-C4 in Sera of Mice Bearing Hybridoma Backpack Tumors by Quantitative ELISA
Ling SUN ; Shengmin HUAG ; Xiaolei WANG ; Yongzhen ZHANG ; Xiaomei CAO ; Kunlong BEN ; Jinju XU
Journal of Kunming Medical University 2001;22(1):30-33
The levels of IgG2b monoclonal antibodies against LD H-C4 in sera of mice bearing hybridoma backpack tumors secreting anti-LDH-C4-IgG2b were detected by quantitative ELISA. The accuracy between batches is 7.04%~l3.30 %, the intra-assay variation is 3.6l%~l0.20%. Standard curveof monoclonal lgG2b was well correlated (r=0.962 884~0.996 795). The sensitivit y of the assay reach e dup to0.0l?mg/L. The present modified ELISA offers a reproducible, se nsitive, specific method in determination of antigen-specific IgG2b antibody in sera.