Bronchiolitis Obliterans ; Child ; Humans

Objective To discuss clinical features and harmfulness of acid-base imbalance in newborn by investigating the changes in blood gas of cord and artery blood,and to establish more appropriate prevention measures. Methods One hundred and ninety-two cord blood and 113 peripheral artery blood of neonates were measured for blood gas analysis by automatic blood gas analysis instrument from America;meanwhile,the concentrations of K~+,Na~+ and Cl~- were detected by biochemical analyzer.Results Among 192 neonates,179 were normal labor and 13 neonates underwent acidosis of different degrees.The incidence was 79.9%.Among the 13 ca-(ses),2 cases had severe asphyxia and both of them got serious mixed acidosis.For all the neonates, 156 cases got acidosis, 84 neonates underwent respiratory acidosis (43.8%), 47 cases with metabolic acidosis (24.5%) and 25 cases with mixed acidosis (13.0%).The left 113 neonates were abnormal labor.Among them,99 cases had acid-base disturbance of different degrees (87.6%). Among the 99 acid-base disturbance cases, 50 cases were single metabolic acidosis (44.2%) and presented the most common changes; the second was single respiratory acidosis, totally 20 cases (17.7%).The third was metabolic acidosis combined with respiratory alkalosis, totally 16 neonates (14.2%).Eleven cases had mixed acidosis (9.7%) and 2 cases were respiratory alkalosis combined with metabolic alkalosis (1.8%).Conclusions While the normal labor neonates still got acidosis of different degrees,the acid-base imbalance of abnormal labor is much more prominent,and the critical neonates often got mixed acid-base imbalance.Serious acid-base imbalance can cause fatal pathological changes in body.The dangers of acidosis and alkalosis can not ignore either. The condition of acid-base imbalance can be judged more accurately by the combination of blood gas analysis and anion gap.The perinatal health cares are important measures to prevent acid-base imbalance.

Aged ; Female ; Humans ; Lymphohistiocytosis, Hemophagocytic ; virology ; Orthobunyavirus ; classification

Brucellosis ; diagnosis ; drug therapy ; Child ; Child, Preschool ; Female ; Humans ; Male

Acupuncture Therapy ; Adult ; Female ; Humans ; Laryngitis ; therapy

The multi-potent differentiation capability of mesenchymal stem cells (MSCs) implies potential to achieve patient-specific regenerative therapy for myocardial infarction. However, it is evident that transplanted stem cells do not survive well in the harsh ischemic microenvironment. Erythropoietin (EPO) has been shown to be a potent inhibitor of neuronal and myocardial apoptosis. Here, we investigate the cell viability and the change of erythropoietin signal pathway of MSCs challenged by hypoxia treatment.Methods MSCs were derived from the bone marrow of 2-week-old Wistar rats and expanded. Hypoxia treatment of cells was performed using a ProOx-C-chamber with the oxygen concentration set to 0.5% for the times required.Results The rate of trypan blue staining was 3.5% ± 0.4% in control group, and 3.9% ± 0.2%, 5.0% ±0.5%, 7.1% ± 0.5% in the groups treated with hypoxia for 24, 48, 72, 96 hours. Western blot analysis suggested the expression of EPO in MSCs was significantly upregulated after 48-hour treatment with hypoxia, which was further confirmed by immunofluorescence staining. There was higher and earlier expression of EPOR. And the expression level of total ERK remained constant during the hypoxic treatment. However, the expression of HIF-1α phosphorylated ERK was significantly upregulated after 24-hour treatment with hypoxia and peaked at 72 hours.Conclusions MSCs is not sensitive to hypoxia insult alone. The components in EPO signal pathway (e. g.EPO, EPOR and P-ERK) is upregulated in MSCs after hypoxic treatment, which suggests that EPO signal pathway plays an important role in the hypoxia-tolerance and paracrine protecting capability of MSCs.

Adult ; Case-Control Studies ; Female ; Humans ; Male ; Nervous System Diseases ; etiology ; physiopathology ; Neural Conduction ; Organophosphate Poisoning ; Pesticides ; poisoning ; Young Adult

Objective:To study the association between rs2235371,rs2013162,rs2235377 SNPs in interferon regulatory factor 6 (IRF6)gene and non-syndromic cleft lip with or without cleft palate(NSCL/P)in Xinjiang Uyghur population.Methods:100 Uyghur NSCL/P patients from Xinjiang were included in the case group and 60 Uyghur inpatients with upper respiratory tract infection were se-lected in the control group.Next,generation sequencing was used,DNA sequencing results were compared with the information on the genome database and genetic analysis were made.Results:There were no significant differences in the frequency distribution of both genotypes and alles when the cases were campared with the controls at the rs2235371,rs2013162 and rs2235377 loci(P >0.05). Above three loci were located in the same block,rs2235371 and rs2235377 loci presents the strong linkage disequilibrium(r2 =0.949, D'=0.974).Possible haplotypes were:CCT >CAT >TAC,and there was no significant difference between the cases and controls in haplotype distribution(P >0.05).Conclusion:Polymorphisms of rs2235371,rs2013162 and rs2235377 in IRF6 gene may be associ-ated with NSCL/P in Xinjiang Uygur people.

Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cytarabine ; administration & dosage ; therapeutic use ; Female ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Male ; Middle Aged ; Prognosis ; Treatment Outcome ; Young Adult

Acute Disease ; Adult ; Female ; Herbicides ; poisoning ; Humans ; Male ; Middle Aged ; Paraquat ; poisoning ; Retrospective Studies ; Young Adult