The trace elements (Mn, Cu, Zn, Fe) andCalcium, Magnesium in aged person's serum oflong-life area and control area were determined. Itwas found that the contents of Manganese, cop-per, and Calcium of aged person's serum in long-life area were significantly higher than those incontrol area (Manganese and Calcium are essentialelements for health and antiaging, Copper is an es-sential trace element to health). In the long-lifearea the contents of six kinds of elements (Mn,Cu, Zn, Fe, Ca, Mg)in the serum have no signifi-cant distinction between the male aged and thefamle aged. In the long-life area the contents of sixkinds of elements (Mn, Cu, Zn, Fe, Ca, Mg )inaged (61～90) person's serum have no significantincrease or decrease with the increase of age.Judged from serum compositions, the agedperson's serum of the long-life area have a betterspectrum of elements. The relationship betweenthe elements in this experiment and antiaging effect was discussed.

Objective To perform molecular evolution analysis of mazEF gene in genome sequenced strains of Escherichia coli and Shigella. Methods Pathway Tools (version 13.5) provided by BioCyc was used to search encoding gene mazF of toxin MazF ( chpA) and encoding gene mazE of antitoxin MazE (chpR) in genome sequenced 10 strains of Escherichia coli, 6 strains of Shigella and 1 strain of unkown Enterobacteria. Then Minimum Evolution method in MEGA4. 1 software was used to analyze molecular evolution of MazE and MazF. Results Encoding gene mazF of toxin MazF was found in 12 strains, and encoding gene mazE of antitoxin MazE was found in 11 strains, while neither mazE nor mazF was found in rest 5 strains. Both mazE and mazF had good conservation in molecular evolution analysis. Conclusions MazEF is the first toxin-antitoxin system found in prokaryotic chromosomes, but not in all strains of Escherichia coli and Shigella. MazEF deletion is associated with antibiotic resistance and it also mediates programmed cell death in bacteria, so MazEF might be a new target for antimicrobial agents.

Objective To analyze molecular evolution and binding free energies in substrates of KPC-2,KPC-5 and KPC-10 carbapenemases.Methods Minimum Evolution method in MEGA 4.1 was used to analyze molecular evolution of KPC-2,KPC-5 and KPC-10 carbapenemases,Dock module in ArgusLab 4.1 was used to perform molecular docking of these 3 carbapenemases to 10 kinds of β-lactams substrates,and calculate binding free energies(△G).Results Ambler Class A β-lactamases with carbapenemase activities were grouped in the same cluster and had good conservation,while ordinary Ambler Class A β-lactamases without carbapenemase activities were groupod in the other cluster.Binding free energies of KPC-2,KPC-5 and KPC-10 carbapenemases were lower to carbapenem antibiotics than the thirdgeneration cephalosporins,while binding free energies to aztreonam and clavulanic acid were of comparatively higher levels.Conclusion Catalytic activities of KPC to carbapenem antibiotics are higher than those to the third-generation cephalosporins,but the activities to aztreonam and clavulanic acid are low.

In the view of physiology,sleep can be described as an important modulatory factor for hormone release,glucose regulation,and cardiovascular function.The circadian rhythms of hormones is mainly associated with sleep,which is largely mediated by neuroendocrine system.Sleep deprivation will disrupt this rhythm,leading to a series of metabolic diseases.We published an article in 《J Clin Endocrinol Metab》 titled Association between sleep duration,daytime nap duration,and osteoporosis varies by sex,menopause,and sleep quality,which suggested that sleep durations of 7-8 h/day,9-10 h/day,and ≥ 10 h/day,as well as longer daytime napping times,tend to present higher risks of having osteoporosis,and this tendency is especially obvious in postmenopausal women reporting good quality sleep.

To explore the characteristic genomics of syndrome of liver-kidney yin deficiency in peripheral blood mononuclear cells of hepatocellular carcinoma (HCC) patients.

ObjectiveTo investigate the therapeutic effects of modified constraint-induced movememt therapy(mCIMT) on upper extremity motor function of stroke patients with hemiparasis, and to compare the effects of mCIMT with those of conventional rehabilitation.MethodsThirty stroke patients with hemiparesis were divided into mCIMT group and control group (conventional rehabilitalion), 15 cases in each group. The mCIMT group received mCIMT (by shaping) in the affected extremity without restriction of movement of intact upper extremity, 6 h a day, five times a week, for two weeks while the control group was treated with traditional rehabilitation in upper-limb and hands functional exercises. Motor activity logs(MAL) score and simple test for evaluating hand function(STEF ) score were measured in two groups before treatment and 2 weeks, 1 month and 3 months after treatment.ResultsThere were improvement in MAL and STEF scores in both mCIMT group and control group 2 weeks after treatment compared with pre-treatment(P<0-01), and the scores of MAL and STEF on post-treatment 1 month and 3 months were higher than those of pre-treatment in mCIMT group (P<0-05, P<0-01), but there was no significant difference in MAL and STEF score between pre-treatment and post-treatment 1 month and 3 months in control group(P>0-05).ConclusionSignificant improvement in the affected hand function could be achieved with modified constraint-induced movement therapy in stroke patients, better than conventional rehabilitation therapy.

Objective To study the diagnostic value of combined detection of serum CEA,CA199,CA125,AFP and fecal occult blood(FOB) in Dukes B stage colon carcinoma.Methods The above indicators in 56 patients with colon carcinoma (colon carcinoma group),35 patients with colonic polyp benign disease and 41 healthy individuals undergoing physical examination were measured.Then the sensitivity,and specificity of single index detection and their combined detection were analyzed.Results The levels and positive rates of CEA,CA199,CA125 and FOB in the colon cancer group were significantly higher than those in the colonic polyp group and healthy control group (P＜0.05),but the AFP level and positive rate had no statistical difference compared with the colonic polyp group.Serum CEA,CA125,CA199 and FOB were selected into the Logistic regression equation.The corresponding areas under the curve (AUC) were 0.745,0.886,0.792 and 0.864 respectively;the positive detection rates were 48.2 ％,35.7 ％,39.3 ％ and 78.6 ％ respectively.The combined detection could increased the specificity,sensitivity and AUC to 87.5 ％,92.1％ and 0.957,which were more superior to that in the single index detection.Conclusion The combined detection of serum CEA,CA199,CA 125 and FOB can provide the powerful basis for early diagnosing colonic cancer.

type of extreme heat with yin asthenia.But discrepancy of CO and EF between type of deficiency of both QI and YIN and type of QI-stagnation and blood stasis all had not statistical significance.Conclusion: In clinic to aim directly at left ventricular contractile dysfunction of senile diabetics,no matter syndrome differentiation belonging to type of deficiency of both QI and YIN,type of QI-stagnation and blood stasis or type of deficiency of both yin and yang,all can benefit after treatment with supplementing QI and activating blood circulation method;but syndrome differentiation belonging to type of extreme heat with yin asthenia advocates not to use supplementing QI and activating blood circulation method.

Objective To investigate the correlation between ankle-brachial index and ultrasonography in lower ex- tremities vascular in type 2 diabetic patients.Methods 218 type 2 diabetic patients were enrolled,and ankle-brachial index(ABI)measurements and uhrasonography in lower extremities vascular were performed in all patients.Lower extremi- ties vascular disease(LEVD)were divided into six species according to the uhrasonography results and scored in accord- ante with its serious degree,then investigate the correlation between ABI and uhrasonography results score.All patients were divided into deferent groups according to uhrasonography results and ABI range,and then compared their deference. Results The lower the ABI,the more serious of the LEVD showed by ultrasonography.ABI and ultrasonic inspection score was significantly negatively correlated(r=-0.65,P

A cross-sectional survey of 3589 adolescents was conducted in three cities from different typical geographical zones of Guangdong province. The median urinary iodine concentrations (MUI) of adolescents in Nanxiong, Guangzhou and Maoming were 286.6,204.1 and 166.0μ/L, respectively. The MUI of all these adolescents Was 231.7μg/L, which was slightly higher than the current World Health Organization recommendation.