The serous anti-corneum antibodies(ACAs)of 50 psoriatic patients and 32 normal con- trols were measured by Biotin Avidim Enzyme Linked Immunosorbent Assay (BA-ELISA).The results showed that ACAs of patients were significantly lower than that of the controls(P

To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X-ray, CT and MRI. Results Post-traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis.

Objective To investigate rhein on podocyte nephrin gene expression and nephrin on glomerular in diabetic nephropa-thy (DN)rats .Methods DN rats mode were made by Intraperitoneal injection of streptozotocin (STZ) 60 mg/kg once .the DN model was divided into 2 groups:rhein treatment group (A) ,model group (B) ,and in addition ,set up a control group (C) .body weight ,kidney weight ,relative kidney weight ,24-hour urinary protein excretion ,serum creatinine ,blood urea nitrogen of rats were detected after 8 weeks of intervention .kidney pathological changes were observed by using light microscope ,electron microscope . immunohistochemical technique used to nephrin protein expression .Results Both the DN rats 24 -hour urinary protein excretion and podocyte nephrin gene expression of group A decreased significantly (P<0 .05) ,improvement in renal function .Conclusion Rhein on DN kidney has a protective effect ,which may be related to its podocyte nephrin gene expression.

Objective: [WT5”,6BZ]To investigate the anti tumor effects of melittin in vitro . Methods: Melittin was isolated and purified from bee venom by gel filtration. The cytotoxicity of melittin against established cell lines(SMMC 7721,BEL 7402 and Hep 3B cell) was determined using by MTT assays. Mitomycin, vincristine and cinobu facini were used as control. The time effect relationship of melittin against SMMC 7721 cell line was studied. Results: Melittin showed the anti tumor effect in a dose dependent matter for tumor cells in vitro ,but there was no time effect relationship. The anti tumor effect of melittin was better than the control. Conclusion: Melittin has marked and quick anti tumor activity in vitro against established cell lines. [

Objective To investigate the influence of long-term blood purification on atherosclerosis(AS) in maintenance hemodialysis(MHD) patients by measuring serum levels of homocysteine(Hcy) and carotid artery intima-medial thickness(IMT).Methods Seventy-two MHD patients visiting our hospital from June 2006 to December 2007,according to their blood purification mode,were assigned to three groups: hemodialysis(HD,n=25),hemodialysis+hemodiafiltration(HD+HDF,n=30),hemodialysis+continuous renal replacement therapy(HD+CRRT,n=17).Another 25 healthy subjects served as controls.Serum Hcy was detected before and after one-year treatment and meanwhile color Doppler ultrasonography was performed to check IMT of carotid artery and atheromatous plaques.Results The serum levels of Hcy,IL-6,MCP-1,hs-CRP in MHD patients were significantly higher than those in healthy controls(P

Objective To investigate and improve the methods of nasal feeding to reduce the occurrence of complication such as diarrhea and vomiting.Methods We separated 140 patients with heavy craniocerebral injury into the control group (50 patients) and the experimental group (90 patients) according to different nasal feeding methods. For the control group traditional method was used. For the experimental group infusion pump was used to control infusion rate. The incidence of complication was compared between two groups. And within experimental group, the same nutrient solutions in respect of type and volume were infused during 10 hours, 16 hours, and 18 hours respectively. The relationship between infusion rate and the occurrence of complication was observed, analyzed and compared to seek for the most suitable enteral nutrition route which incurred the least complication.Results The incidence of diarrhea and vomiting in the experimental group was lower than that of the control group. And there were no significant differences in the occurrence of complication by using infusion pump at three different infusion rates within the experimental group. Conclusions The application of infusion pump in nasal feeding can reduce the incidence of complication and infusing nutrient solution at a slow rate was not necessary.

Isocitrate dehydrogenases(IDHs)are considered as key enzymes in the tricarboxylic acid cycle. Recurrent mutations in the IDH1 and IDH2 genes are recently found in several human cancers. Those point mutations specifically affect IDH1 and IDH2 active site arginine residues and confer a neomorphic enzyme function of directly catalyzing α-ketoglutarate(α-KG)to R-2-hydroxyglutarate(R-2-HG). R-2-HG can com-petitively inhibits α-KG-dependent enzymes and may therefore contribute to the occurrence and development of tumor. In addition,Mutation status of IDH1 and IDH2 are closely relative to the progress and prognosis of cer-tain tumor. Thus IDH1 and IDH2 are considered to be promising biomarkers for early diagnosis and prognosis and targeted therapy.

Objective To describe the relationship between ambient air pollutants(TSP,SO2,NOx) and the level of children’s lung function (FVC,FEV1,MMEF) in China. Methods The papers on ambient air pollution and children lung function published from 1985 to 2006 were collected and 11 of them were selected according to the following criteria: children aged 7-15; local air quality monitoring results were reported; strict quality control were taken when testing children lung function; the results were expressed by the average of measure. Results The selected papers included the results of 7 cities in China. Among them,the result of 6 cities showed that the levels of children lung function were significantly lower in the heavy air pollution areas compared with that in the light pollution areas. According to the papers,the average levels of ambient air TSP were in the range of 0.084 mg/m3-0.835 mg/m3,SO2(cSO2) were 0.013 mg/m3-0.929 mg/m3,NOx were 0.044 mg/m3-0.229 mg/m3,there was a significant negative correlation between the levels of TSP and SO2 and children FVC and FEV1,as well as the levels of NOx and children MMEF,the correlation coefficients were as follows,-0.797(t=-4.384,P=0.001) between TSP and FVC,-0.693(t=-4.190,P

Objective To evaluate the role of angiocadiography(ACG)in the diagnosis and management of complex/complicated congenital heart disease(CHD).Methods A retrospective study of ACG findings in 360 cases with complex/complicated CHD was performed with a comparision to that of echocardiography(Echo)and related clinical examination.Results The present series of CHD cases included pulmonary atresia with ventricular septal defect in 75 cases,double outlet of right ventricle in 62 cases,Fallot's tetralogy in 60 cases,single ventricle in 52 cases,transposition of the great arteries in 42 cases,tricuspid valve atresia in 15 cases,coronary abnormality in 6 cases,total abnormal pulmonary venous connection in 5 cases,total endocardial cushion defect in 5 cases,persistent truncus arteriosus in 4 cases,pulmonary atresia with normal ventricular septum in 3 cases,other disorders in 7 cases,and postsurgical operation in 24 cases.ACG was superior to that of Echo in demonstrating the abnormalities of systemic,pulmonary,and coronary arteries and their branches of complex/complicated CHD as well as measuring the pressure of pulmonary artery,vein,and systemic-pulmonary collateral vessels.Conclusion In the diagnosis and differential diagnosis of knotty cases with complex and complicated CHD,particularly in the demonstration of full view of systemic,pulmonary,and coronary arterial branches and accurate measurement of pulmonary arterial pressure/resistance,and atrial,ventricular,and systemic arterial pressure,ACG(including DSA)still plays an important and irreplaceable role.