The cultivation of practice ability of public health personnel was studied, in order to adapt to the new public health system requirements in practical ability training. With integrating the teaching contents, setting experiment course independently, implementing 'Process-experiment', establishing the experimental skills evaluation system of the formative assessment and summative assessment, public health personnel's practice ability has been improved.

Objective To further understand the etiology,definition,clinical manifestation,prognosis and treatment of cutaneous leukocytoclastic vasculitis.Method Retrospective study of23patients with leuko-cytoclastic vasculitis followed up at Peking Union Medical College Hospital from1990to2001.Results Twenty patients were classified as hypersensitive vasculitis,one of whom was diagnosed as Wegeners granulo-matosis2.5years later,two were diagnosed as urticarial vasculitis,and one was systemic lupus erythematosus.Conclusion Cutaneous leukocytoclastic vasculitis,which is usually a benign syndrome,may be caused by in-fection or drugs.Its main clinical manifestaions are skin symptoms.

Objective To study the real releasing of fluorine (F) and its effects on environmen in coalburning-borne fluorosis areas.Methods The coal,clay,briquette,cinder,fresh corn and roasted corn samples from 24 households were collected systematically in Zhaotong of City,Yunnan Province,and the F concentration in all samples was measured using the combustion hydrolysis/fluoride-ion selective electrode method,and the released amount and rate of F in briquette was calculated,and the relation between the released amount of F from briquette and the increase of F in roasted corn was analyzed.Results The F concentration of clay ranged from 266.4 to 2 536.7 mg/kg with a median of 1 087.1 mg/kg;the F concentration of briquette ranged from 115.9 to 635.0 mg/kg with a median of 308.8 mg/kg.The released amount of F in briquette ranged from 63.5 to 432.2 mg/kg with a median of 248.2 mg/kg.The releasing rate of F in briquette ranged from 30.3％ to 91.5％ with a average of 73.8％.The F concentration in fresh corn ranged from 0.43 to 2.00 mg/kg with a median of 1.31 mg/kg;the F concentration in roasted corn ranged from 2.21 to 44.88 mg/kg with a median of 10.49 mg/kg.The increased amount of roasted corn ranged 0.90 to 44.46 mg/kg.There was a significant correlation between the emission amounts of F from briquette and the F concentration in binder clay (r =0.714,P ＜ 0.01),but no correlation between the released amount of F from briquette and the increasing of F in roasted corn (r =-0.155,P ＞ 0.05).Conclusion The clay is an important source of F pollution,and the F increase in roasted corn is not only determined by the released amount of F in briquette,but also by several factors.

To improve the preparation speed and quality of moxa cone, a mould for preparation of moxa cone was designed and applied in moxibustion. It consists three parts: a foundation (a flat-bottomed circular cylinder hollow in center), a moxa cone forming tube (a hollow column encircled by two semi-cylinders with symmetrical shape and size, and was inserted in the internal cavity of the foundation); and a compression rod (a solid cylinder used for compressing moxa placed in moxa cone forming tube). The mould is practical, simple, and convenient and can effectively speed up the preparation of moxa cone, and it is safe in application. Moreover, the obtained moxa cones are regular in shape, uniform in dosage and size, dense in texture, and convenient to pack, store and carry.

Objective To investigate the feasibility of methotrexate incision and different methods of administration combined with hysteroscopic surgery for cesarean scarpregnancy (CSP).Methods Retrospective analysis of 106 patients with CSP in our hospital from January 2001 to December 2015 were.The patients were divided into two groups, group A of 66 cases: methotrexate ( MTX) incision and systemic administration combined with hysteroscopic surgery group B of 40 cases: methotrexate combined with hysteroscopy surgery.The operation time, intraoperative blood loss, preoperative hospital stay, the time of blood HCG decreased to normal, the total amount of MTX and the abnormal rate of liver function were compared between the two groups, and the factors affecting the prognosis of CSP were analyzed.Results The preoperative hospitalization time and abnormal liver function of the two groups were compared, the difference was statistically significant(P<0.05).A group of patients with success and failure, blood HCG before treatment, gestational sac and bladder wall thickness of the myometrium (MT), the difference was statistically significant (P<0.05).The median gestational sac diameter ( GS) of patients with successful treatment was 17.1 mm, and the median number of patients was 6.2 mm, and the difference was statistically significant between the two groups ( GS ) , the difference of GS between the two groups was statistically significant ( P<0.05 ) . Conclusion Methotrexate incision and systemic administration methods combined with hysteroscopy treatment compared with systemic administration combined with hysteroscopy is a safe and effective method and the hospitalization time is short, but the MT >3 mm, before treatment, blood HCG≤20000IU/L, GS≤40 mm CSP more secure.

AIM: To investigate the effects of lipid-induced insulin resistance on plasma resistin and ghrelin levels in awake rats. METHODS: A hyperinsulinaemic-euglycaemic clamp was established in awake chronically catheterized rats. Two groups of rats were studied either with a 4 h intraarterial infusion of lipid/heparin or saline. Insulin-mediated peripheral and hepatic glucose metabolism was assessed by insulin clamp combined with [~3H]-glucose infusion, and plasma resistin and ghrelin concentrations were examined before and after clamp with ELISA. RESULTS: During clamp, there was a significant increase in plasma free fatty acid (FFA, P

Objective:To observe the expression of cell adhesion molecule CD44 in lung tissue of rats with asthma.Methods:32 SD rats were randomly divided into 4 groups:the normal group and the model group,8 rats in each group.The asthmatic model was established by repeated inhalation of ovalbulium.The expression of CD44 in lung tissue of rats was detected by semi-quantitatively reverse transcription-polymerase chain reaction(RT-PCR) and by means of immuno-histochemical staining.Differential leukocytes counts(neutrophils,eosinophils,and lymphocytes) in bronchoalveolar lavage fluid(BALF) were performed after staining with wright staining.Results:The expressions of CD44 increased one week after OVA challenge(P

Objective To explore the substantial results of using comprehensive practical teaching mode and holistic nursing thinking method in the course of teaching fundamentals of nursing science,and then improve the vocational quality and ability of nursing students.Methods Randomly determined two classes as objectives: experiment class and control class.control class was adopt traditional practical teaching mode,experiment class was adopt comprehensive practical teaching mode in small community.Results Ability of standard operation,communication and knowledge were significant higher in experiment class than those of in control class.Conclusions Comprehensive practical teaching mode in small community has actual significance for strengthen the comprehendsive ability of nursing students.

Objective To explore the expressions of smooth muscle actin (SMA) - a and CD44 mRNA in the lungs of rats with asthma in different stages. Methods The asthmatic rat model was established by repeated exposing the animal to aerosolized ovalbumin (OVA). After being sensitized, the rat was subjected to laboratory test. Semi - quantitatively reverse transcription- polymerase chain reaction (RT- PCR) was used to analyze CD44 and a - SMA mRNA of lung tissue. Differential leukocytes(neutrophils, eosinophils, and lymphocytes) counts in bronchoalveolar lavage fluid (BALK) were performed after Wright staining. Results The expressions of CD44 and ? - SMA mRNA increased after OVA challenge 1 week (P

Objective To explore the effect of status epilepticus(SE) on learning and memory and expression of phosphorylated c-AMP response element binding protein(pCREB) in hippocampus in developmental rats.Methods Developmental male SD rats were assigned randomly to two groups:the SE rats,induced by intraperitoneal injection of pentylenetetrazole(PTZ);normal saline(NS) control group,received saline intraperitoneal.Learning and memory test with the Morris water maze and Y maze were performed at 7 days after SE.After testing,a subset of rats were killed and the brain was evaluated for pCREB immunostaining,an important transcription factor underlying learning and memory.Results In SE group,the mean escape latency of searching the platform significantly prolonged(P