The serous anti-corneum antibodies(ACAs)of 50 psoriatic patients and 32 normal con- trols were measured by Biotin Avidim Enzyme Linked Immunosorbent Assay (BA-ELISA).The results showed that ACAs of patients were significantly lower than that of the controls(P

To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X-ray, CT and MRI. Results Post-traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis.

Objective To evaluate the effect of the Danhong injection combined with irbesartan in treatment ofearly diabetic nephropathy. Methods 64 patients with early diabetic nephropathy were randomly divided into two groups and the control group 32 cases,two groups were given control of blood sugar and ensure water and electrolyte balance,support therapy,in which 32 cases were also observed red Danhong injection,Ecuador irbesartan treatment,2weeks before evaluation. Results The total effective rate in treatment group was higher than the control group( x2 =6.38 ,P ＜0. 05) ,after 12 weeks of treatment,two groups of urinary protein decreased significantly compared with before treatment, including observation of urine protein was ( 0. 6± 0. 1 ) g/24h, the control group was ( 0. 7 ± 0. 3 ) g/24h,and decreased urine protein level of the observation group was better than the control group( t = 2. 74,P ＜ 0. 05 ). And in the course of treatment, side effects and serious adverse events were not found. Conclusion Danhong injection combined irbesartar, could improve the clinical symptoms, reduce microalbuminuria in treatment of early diabetic nephropathy is worthy of wider application.

Objective To observe the connections between enkephalin immunoreactive(ENK-ir) terminals and ?-aminobutyric acid immunoreactive(GABA-ir) neurons in the rostral portion of the nucleus tractus solitarious(rNTS) of the rat. Methods Double-immunofluorescent labeling method and the pre-embedding immunohistochemical staining combined with immuno-gold particles labeling technique for electron microscopic detection were used in the present study. Results Under the laser scanning confocal microscope,dense ENK-ir fibers and terminals and some GABA-ir neurons were observed in the rNTS.Close contacts between ENK-ir terminals and GABA-ir cell bodies were also detected.By using the electron microscopic technique,ENK-ir reaction products were found to be mainly localized on the surface of round clear vesicles and dense-cored vesicles in the axon terminals.ENK-ir terminals formed symmetric,which was a dominant pattern,and asymmetric synaptic connections with GABA-ir and immunonegative cell bodies and dendrites.Conclusion The ENK-ir terminals might take part in the transmission and regulation of the taste information in the rNTS through inhibiting,exciting the GABAergic neurons or inhibiting directly the activity of neurons within the rNTS.

Objective To describe the relationship between ambient air pollutants(TSP,SO2,NOx) and the level of children’s lung function (FVC,FEV1,MMEF) in China. Methods The papers on ambient air pollution and children lung function published from 1985 to 2006 were collected and 11 of them were selected according to the following criteria: children aged 7-15; local air quality monitoring results were reported; strict quality control were taken when testing children lung function; the results were expressed by the average of measure. Results The selected papers included the results of 7 cities in China. Among them,the result of 6 cities showed that the levels of children lung function were significantly lower in the heavy air pollution areas compared with that in the light pollution areas. According to the papers,the average levels of ambient air TSP were in the range of 0.084 mg/m3-0.835 mg/m3,SO2(cSO2) were 0.013 mg/m3-0.929 mg/m3,NOx were 0.044 mg/m3-0.229 mg/m3,there was a significant negative correlation between the levels of TSP and SO2 and children FVC and FEV1,as well as the levels of NOx and children MMEF,the correlation coefficients were as follows,-0.797(t=-4.384,P=0.001) between TSP and FVC,-0.693(t=-4.190,P

Objective To evaluate the role of angiocadiography(ACG)in the diagnosis and management of complex/complicated congenital heart disease(CHD).Methods A retrospective study of ACG findings in 360 cases with complex/complicated CHD was performed with a comparision to that of echocardiography(Echo)and related clinical examination.Results The present series of CHD cases included pulmonary atresia with ventricular septal defect in 75 cases,double outlet of right ventricle in 62 cases,Fallot's tetralogy in 60 cases,single ventricle in 52 cases,transposition of the great arteries in 42 cases,tricuspid valve atresia in 15 cases,coronary abnormality in 6 cases,total abnormal pulmonary venous connection in 5 cases,total endocardial cushion defect in 5 cases,persistent truncus arteriosus in 4 cases,pulmonary atresia with normal ventricular septum in 3 cases,other disorders in 7 cases,and postsurgical operation in 24 cases.ACG was superior to that of Echo in demonstrating the abnormalities of systemic,pulmonary,and coronary arteries and their branches of complex/complicated CHD as well as measuring the pressure of pulmonary artery,vein,and systemic-pulmonary collateral vessels.Conclusion In the diagnosis and differential diagnosis of knotty cases with complex and complicated CHD,particularly in the demonstration of full view of systemic,pulmonary,and coronary arterial branches and accurate measurement of pulmonary arterial pressure/resistance,and atrial,ventricular,and systemic arterial pressure,ACG(including DSA)still plays an important and irreplaceable role.

Objective To explore a new bio-dynamic differential equation to establish gene regulatory network based on the time-space of gene expression.Methods According to bio-dynamic competitive model and differential equation,a new time-space Lotka-Volterra differential equation was established and was applied to time-order expression data.Results The model was applied to set up the regulation network of yeast genes.The regulatory relation was found and compared with the experiment results.Conclusion The results from the new model were almost consistent with the real situation.So this model can remedy the shortage of other models.It's a new valuable differential equation model.

OBJECTIVE To investigate the distribution and resistance rate of clinical isolates from neonatal sepsis in Hubei hospitals.METHODS The antimicrobial susceptibility of clinical isolates from(neonates) blood culture derived from sixteen hospitals in Hubei from 2000 to 2004,was retrospectively analyzed and tested by KirbyBauer(method).RESULTS A total of 1 019 strains were isolated from blood specimens,Gram-positive and Gram(-negative)(bacteria) accounted for 93% and 7% of these isolates,respectively.The main isolates were coagulase(-negative) staphylococci(CNS,79.8%),Enterococcus spp(7.0%),Staphylococcus aureus(SAU4.5%),Klebsiella spp(1.7%),Stenotrophomonas maltophilia(1.2%),and Escherichia coli(0.9%).71.3% CNS and 19.6% SAU were meticillin resistant,46.5% enterococci resistant to ampicillin and almost strains were susceptible to(vancomycin)(except E.gallinarum).ESBLs were detected in isolates of 56% E.coli and 35% K.pneumoniae.S.maltophilia was susceptible to trimethoprim(-sulfamethoxazole) and levofloxacin with resistance rate of 8.3% and 0%.In strains of other Gram-negative rods,resistance rate to imipenem,amikacin and fluoroquinolones were detected between 0% to 15%,and to cephalosporins Ⅰ-Ⅳ were between 20% to 70%.CONCLUSIONS Gram-positive cocci are important pathogens in neonatal sepsis,antimicrobials should be used on the result of(antimicrobial) susceptibility test.It is more important to reduce health care-associated(nosocomial) infections.

Objective To explore the role of halofuginone in immune regulation,observe the effects of halofuginone on maturation of dendritic cells and immune tolerance during homograft heart transplantation of rats,in order to provide new ways and means to treat transplantation tolerance and to explore its possible mechanism.Methods Rat bone marrow derived dendritic cells were isolated and the effects of halofuginone in LPS-treated DCs were investigated by flow cytometry;heart transplantation model of rats were established and the effect of halofuginone stimulated dendritic cells in the immune tolerance by pathological analysis and mixed lymphocyte reaction were observed.Results Flow cytometry results show that the expression of cell surface CD80 CD86,OX62 antigens were not high in control group,their expression increased significantly when stimulated by LPS for 24h,and decreased when stimulated by both Changshan ketone and LPS for 24h,and even lower than their expression levels in blank group,which showed that Changshan ketone has the ability to inhibit the maturation of dendritic cells induced by LPS.In rat heart transplantation model,dendritic cells stimulated with Changshan were injected into recipient rats in vivo,and receptor of rat transplantation rejection decreased significantly,which showed that dendritic cells stimulated with Changshan ketone may be used as a new method for cell therapy in treatment of transplant rejection.Conclusion This study systematically observes that halofuginone can inhibit the maturation of rat bone marrow-derived dendritic cells and lymphocyte proliferation for the first time in vivo and in vitro.