Objective To investigate the impacts of Xuebijing injection on systemic inflammatory response and immune function of patients with acute exacerbation of severe chronic obstructive pulmonary disease (AECOPD). Methods A prospective randomized controlled trial (RCT) was conducted. Forty patients with severe AECOPD were divided into control group and Xuebijing group in accordance with the random number table, each group 20 cases. Both groups were treated by routine conventional basic therapy of severe AECOPD including anti-infection, phlegm-expelling formula, bronchodilators and mechanical ventilation, etc, while in Xuebijing group, intravenous drip of Xuebijing (100 mg, twice a day for 5 days) was additionally used. The changes of data of arterial blood gas analysis, blood routine examination, C-reactive protein (CRP), procalcitonin (PCT), immune function, and acute physiology and chronic health evaluation system Ⅱ (APACHE Ⅱ) score before and after therapy were observed and compared between the two groups. The length of stay in hospital, duration of mechanical ventilation, and prognosis were also compared between the two groups. Besides, according to the difference in APACHE Ⅱ score, all the patients were divided into APACHEⅡscore≥15 score group (18 cases) and APACHEⅡscore<15 score group (22 cases), and the immune function were compared between the two groups. Results ①The immune function was descended and disordered in patients with severe AECOPD. Compared with the < 15 score group, the expressions of CD45+, CD3+, CD4+, CD4+/CD8+ ratio, B-lymphocyte, natural killer cell (NK cell) in ≥ 15 score group were significantly lowered [CD45+(×106/L):663.92±100.61 vs. 1 289.92±169.38, CD3+(×106/L):342.05±108.93 vs. 882.37±172.56, CD4+(×106/L):205.96±63.97 vs. 486.24±108.64, CD4+/CD8+ratio:0.76±0.49 vs. 1.32±0.57, B-lymphocyte (×106/L):124.77±32.72 vs. 166.06±48.02, NK cells (×106/L): 186.47±39.57 vs. 243.51±44.72, all P < 0.05]. There was no statistically significant difference in the expression of CD8+ between the ≥ 15 score group and < 15 score group (P > 0.05). ② Compared with those before therapy, the pH value, oxygenation index, arterial partial pressure of carbon dioxide (PaCO2), white blood cell count (WBC), CRP, PCT were significantly improved after therapy in both control and Xuebijing groups. Compared with those in the control group, WBC, CRP, PCT were significantly lowered in Xuebijing group [WBC (×109/L): 10.29±3.83 vs. 12.69±3.42, CRP (mg/L): 9.06±4.19 vs. 15.26±4.22, PCT (ng/L): 0.18±0.21 vs. 0.42±0.24, all P < 0.05]. There were no statistically significant differences in pH value, oxygenation index and PaCO2 between two groups (all P>0.05). The degrees of improvement of CD45+, CD3+, CD4+, CD8+ and CD4+/CD8+ ratio were more remarkable in Xuebijing group after treatment than those in control group [CD45+ (×106/L): 1 079.38±153.86 vs. 1 015.63±157.11, CD3+ (×106/L): 652.05±100.05 vs. 596.81±106.85, CD4+ (×106/L): 358.92±67.53 vs. 329.99±72.61, CD8+ (×106/L): 205.73±35.19 vs. 230.41±39.74, CD4+/CD8+ratio: 2.16±0.63 vs. 1.52±0.54, all P < 0.05]. The B-lymphocyte and NK cell before treatment and after treatment showed no statistical significant differences between the two groups (all P > 0.05). Compared with the control group, the duration of mechanical ventilation (hours: 56.25±22.87 vs. 69.45±26.59) and the length of stay in hospital (days: 11.00±0.74 vs. 14.00±2.06) were shortened, and APACHE Ⅱ score was significantly lowered in Xuebijing group (8.21±2.97 vs. 12.08±3.12, P < 0.05). The numbers of multiple organ failure and dead patients in Xuebijing group were less than those of control group, but no statistical significant differences were found (both P > 0.05). Conclusion Xuebijing injection for treatment of patients with severe AECOPD can ameliorate inflammatory response, improve immune function, shorten the duration of mechanical ventilation and the length of stay in hospital, and decrease the risk of death.

Objective To understand the clinical distribution and drug resistance situation of112 strains of Acinetobacter Bau-mannii isolated from the patients′sputum samples in cerebral surgery ICU in order to provide the basis for the rational use of anti-bacterial drugs in clinical therapy.Methods 112 strains of Acinetobacter Baumannii were performed the retrospective analysis.The VITEK2-Compact automatic microbiological analyzer was adopted to conduct the bacterial identification and the drug susceptibility test and the K-B method was combined as the supplement control.The Whonet 5.6 software was used for conducting the statistical analysis.Results Among 112 strains of Acinetobacter baumannii,75 strains(67.0%)were multi-drug resistant,8 strains(7.1%) were pan-resistant.The antimicrobial susceptibility tests showed that the resistant rates of Acinetobacter baumannii to mezlocillin, piperacillin and nitrofurantoin reached up to 100%.Acinetobacter Baumannii had the lower resistant rate to polymyxin B (2.7%), the resistant rates to minomycin and ceperazone/sulbactam were 4.5% and 10.7% respectively.Conclusion The drug resistance phenomena of Acinetobacter Baumannii in the cerebral surgery department ICU is serious.Minomycin,polymyxin B and ceperazone/sulbactam have the better antibacterial activities to clinically isolated Acinetobacter Baumannii and may be the alternative drugs for therapy.Clinic should strengthen the monitoring and rationally use the antibacterial drugs.

Arrhythmias, Cardiac ; therapy ; Biomedical Research ; methods ; standards ; Catheter Ablation ; Child ; China ; Drug Therapy ; Humans

Culture ; Medicine, Chinese Traditional ; Research ; Translations

OBJECTIVE: To develop a method for determination of the content of Dexamethasone acetate in Compound tretinoin ointment by RP-HPLC. METHODS: The separation was performed on C18 column with methanol: water (70∶30) as a mobile phase, wavelength of UV detector at 240nm. RESULTS: The linear range of Dexamethasone acetate was within 0.028 3～0.849 0?g(r=0.999 9). The average recovery of Dexamethasone acetate was 101.13%, with RSD at 1.28%. CONCLUSION: The method is simple, rapid and capable of producing good separation results. It can be applied to the quality control of Compound tretinoin ointment.

For 10 years recently,China has implemented the strategy of "rejuvenating technology,talents for the first",and China's higher education has made improvement significantly: the number of student in-class increases drastically,the gross enrollment rate of higher education reached the level of popular education.Kunming Medical College,in this particular historical period of development by accelerating the construction and structural adjustment, strengthening management,taping potential,integrating use of resources,has better dealt with the enormous pressure and the challenge caused by the school's operating scale's nearly five times increase,and has ensured the quality of student training;In the new historical period of development, Kunming Medical College will continuously change education ideas,achieve the leap development from teaching university to teaching and research university positively.

OBJECTIVE:To establish an HPLC method for the determination of Scutellarin content in Kang’aiping pills. METHODS: The chromatographic separation was performed on DiamondTM C18 column (250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water-acetic acid glacial(20∶80∶1) at a flow rate of 1.0 mL?min-1. The column temperature was set at 45 ℃ and the detection wavelength was set at 335 nm. RESULTS: The linear range of scutellarin was 0.267 5～2.140 0 ?g(r=0.999 9) and the recovery rate was 98.33%(RSD=1.34%,n=6).CONCLUSION: This method is simple, practicable, accurate and reliable, and it can be used for the quality control of Kang’aiping pills.

The present professionalism of higher vocational nursing students was investigated through questionnaire forms from 5 aspects including commission to career, faithfulness to one's duty, etc to address the importance of education for professionalism. A series of suggestions are also put forward from the aspects such as training professional interest, construction of professional images, the study of medical legislations, and the learning of nursing Professionalism.

Objective To study the therapeutic and preventive effect of acupuncture therapy on acute ischemic cerebrovascular syndrome (AICS) and result of transcranial Doppler (TCD). Methods The 140 cases were randomized into acupuncture group and control group, 70 cases in each, to observe the clinical therapeutic effect and to use TCD for comparing the result before and after the treatment. Results The total effective rate of the acupuncture group and the control group was 97.14% and 55.71% respectively. The result of acupuncture group was significantly superior to that of the control group (P

OBJECTIVE:To establish an HPLC method for the determination of acrivastine in human plasma and study its pharmacokinetics in healthy male volunteers. METHODS:The plasma sample was extracted with acetonitriles and concentrated and quantified with external reference method on a Symmetry ShieldTMPR18 column. The mobile phase was a mixture of acetonitrile-0.2% triethylamine (adjusted pH to 6.8?0.2 by dilute phosphoric acid solution,25∶75) at a flow rate of 1.0 mL?min-1. The column temperature was 35 ℃. The detection wavelength was set at 250 nm. RESULTS:The linear range of acrivastine was 4.687 5～600 ng?mL-1(r=0.999 4). The methodological recovery was 96.14%～98.89%; the intra-day RSD was 1.60%～3.00% and inter-day RSD was 2.03%～6.98%. The concentration-time pharmacokinetic parameters after oral administration of acrivastine capsules were in line with the one-compartment mode. CONCLUSION:The method is simple,sensitive,accurate with high recovery,and it is applicable for the concentration determination and clinical pharmacokinetic study of acrivastine.