Objective:To investigate the reversal effect of MDR1 and MRP antisense oligodooxynucleotide (ASODN) on adriamycin-resistant SGC7901/ADM cells. Methods:SGC7901/ADM cells were transfected by MDR1 or MRP ASODNs or the combination of two. Then the mRNA expressions of proteins MDR1 and MRP were checked by RT-PCR,and the expressions of P-gp and MRP by immunocytochemistry;the intracellular fluorescence intensity of Rhodamine 123 in these cells were determined by flow cytometry;and the sensitivity of these cells to adriamycin,carboplatin and other anticancer drugs were determined by MTT assay. Results:The expression of MDR1mRNA and MRP mRNA in SGC7901/ADM cells decreased at 12h after transfection of ASODNs,decreased to the lowest level at 24h and returned to the level before transfection after 48h. The expression of P-gp and MRP decreased significantly at 48h after transfection with ASODNs compared with the control cells. The retention of Rhodamine 123 in SGC7901/ADM cells was significantly higher than that before transfection, and the intracellular fluorescence intensity significantly increased in ASODNs cotransfection SGC7901/ADM cells, compared with those transfected by MDR1 or MRP ASODNs respectively;In addition,the sensitivity of SGC7901/ADM cells to adriamycin, carboplatin and other anticancer drugs obviously increased after cotransfection of MDR1 and MRP ASODNs compared with transfection of MDR1 or MRP ASODN respectively. Conclusion:The transfection of MDR1 or MRP ASODN can partly reverse the multidrug resistance of gastric glandular carcinoma cells SGC7901/ADM,while cotransfection of MDR1 and MRP ASODNs can significantly reverse drug-resistance of these tumor cells.

Objective To establish a method for determining the content of 4’-O-?-D-glucosyl-5-O methylvisamminol in Ganmao Qingre Granule by HPLC. Method The seperation was performed in Hypersil C18 colunm (4.6 mm?250 mm, 5 ?m) with the mobile phase of methanol-water (44∶56). The flow rate was 1.0 mL/min and the wavelength was set at 292 nm. Results The calibration curves was linear in the range of 4.608～46.08 ?g/mL for 4’-O-?-D-glucosyl-5-O-methylvisamminol (r =0.999 9). The average recovery was 97.9%, RSD=0.71% (n=6). Conclusion The method is simple, reproducible and can be used for the quality control of Ganmao Qingre Granule.

Objective To observe effect of baicalin on VEGF, BMP-2 and IL-1βexpression in rat mobile dental periodontitis maintained phase, and explore the mechanism of baicalin on periodontal tissue remodeling.Methods 80 SD rats were randomly divided into four groups,20 rats in each group.The blank group weren’t giren any treatment, model group were built with periodontitis, control group and observation group were built with periodontitis and then tooth movement model.The observation group were given baicalin, blank group and control group were given the same volume of saline.The rats were killed at 1d, 3d, 6d, 12d, 18d, then samples of the maxilla were taken to make tissue sections and immunohistochemical staining. VEGF, BMP-2 and IL-1βexpression were observed in periodontal tissue.Results The BMP-2 expression isn’t obvious in blank group, but was obvious in model group, and gradually reduced at the end of the afterburner; BMP-2 expression in experimental group were higher than that in control group at the same point ( P<0.05 ) .The VEGF expression in control group and experimental group gradually reduced at the end of the afterburner; VEGF expression in experimental group were lower than that in control group at the same point ( P<0.05 ) .The IL-1βexpression wasn’t obvious in blank group, but was obvious in model group.The IL-1βexpression in control group and experimental group gradually reduced at the end of the afterburner;IL-1βexpression in experimental group were lower than that in control group at the same point ( P<0.05 ) .Conclusion Baicalin can significantly increase the expression of IL-1βin periodontal tissues of periodontitis rats after moving teeth, reduce inflammation, inhibit bone resorption, and also increase BMP-2 and VEGF expression, promote periodontal vessel formation and maturation, which may be one of the mechanisms of baicalin to promote periodontal tissue remodeling in periodontitis rats at holding phase after orthodontic tooth movement.

Cervical cancer is one of the commonly malignant neoplasms for women in our country.It's etiology has known by us through hard working.Cervical cancer is the result of many factors:infection with oncogenic human papillomavirus(HPV)types is the necessary cause of cervical cancer,but not the sufficient cause,and other exogenous and endogenous factors are in conjunction with HPV,such as herpes simplex virusII and chlamydia trachomatis infection,activation of oncogene and inactivation of anti-oncogene,sexual behavior,parity,oral contraceptive(OC)use,smoking and So on.

The present professionalism of higher vocational nursing students was investigated through questionnaire forms from 5 aspects including commission to career, faithfulness to one's duty, etc to address the importance of education for professionalism. A series of suggestions are also put forward from the aspects such as training professional interest, construction of professional images, the study of medical legislations, and the learning of nursing Professionalism.

Objective:To discuss the application of microsimulation model in forecasting the change of medical insurance risk-pooling fund in Kunming city.Methods: Taking the SAS8.2 software as the platform,we constructed a microsimulation model for urban employees' medical insurance in Kunming city using computer simulation technology and ARENA simulation software.Results: The construction process included 4 steps:(1) to confirm the simulation object;(2) to construct the micro data file;(3) to design the system and to program;(4) to run the program and forecast the result.The system mainly consisted of population status module,action module and policy module.Conclusion: The present model complies with the latest type distribution of employees in Kunming,and its simulation result can reflect the effectiveness of medical insurance policy in recent years.

Corneal collagen cross-linking (CXL) is a photochemotherapy for keratoconus, keratectasias and infectious keratitis.Corneal collagen fiber infiltrated with riboflavin, a photosensitizer which generates reactive oxygen species when activated by UVA at 370nm, was induced to form the crosslinks in corneal stroma.Corneal collagen cross-linking alone or combined with medicine therapy could be used to treat fungal keratitis, improve the cure rates, and reduce the complications and the demands of corneal transplantation surgery.The purpose of this paper is to review the basic principle, procedure, laboratory researches, clinical applications and the safety about this treatment.

Cervical cancer is the most common malignant cancer in reproductive organ among females in China, particularly in rural areas. Primary prevention [human papillomavirus (HPV) vaccination] was not performed on a large scale, according to a popula-tion-based study in China. Therefore, we aimed to develop a secondary cervical cancer prevention model. Such model would be suit-able for national conditions in China, scientific and reasonable, of low cost, and ideal for popularization in rural areas. Jing'an county in Jiangxi province was the high risk area of cervical cancer in China. The methods of early diagnosis and treatment effectively reduced the mortality and the detection rates of cervical cancer for 40 years. The characteristics of theJing'an modelwere gradually devel-oped.Jing'an modelcan be used for cervical cancer prevention and control in China's rural areas and can be applied in other develop-ing areas worldwide.

Gene Expression Profiling ; Humans ; Laser Therapy ; Lung Neoplasms ; genetics ; Microdissection ; methods ; RNA, Neoplasm ; isolation & purification

Diagnosis, Differential ; Granuloma, Lethal Midline ; diagnosis ; therapy ; Humans ; Infant ; Male