L1 cell adhesion molecular is a type Ⅰ membrane glycoprotein of immunoglobulin superfamily. L1 is expressed mainly in nervous system and plays important roles in nervous system development, learning and memory. L1 cytoplasmic domain (L1CD) is important for L1 function by mediating signaling. To investigate the molecular mechanisms of signaling mediated by L1CD, yeast two-hybrid assay was carried out to screen the human brain cDNA library using L1CD as the bait. After sequence analysis and BLAST, several candidates were identified. One candidate is PAX6 transcription factor. L1CD and PAX6 cDNA were cloned in expression vectors and cotransfected into COS-7 cells. The interaction between L1CD and PAX6 was verified by co-immunoprecipitation assay. These preliminary results suggest that L1CD may be involved in transcription regulation.

Objective To observe the clinical efficacy of plum-blossom needling combined with Deanxit for the treatment of depression or anxiety in chronic tension headache patients. Methods Sixty chronic tension headache patients with depression and/or anxiety were randomized into treatment group and control group , 30 cases in each group. The treatment group was treated by plum-blossom needling combined with oral use of Deanxit , and the control group was treated only by oral use of Deanxit. After treatment for 4 weeks , the scores of headache symptom, Hamilton Depressive Scale (HAMD), and Hamilton Anxiety Scale (HAMA) of the two groups were observed. Results After treatment , the scores of headache symptom , HAMA and HAMD in all patients were decreased (P < 0 . 05 compared with those before treatment), and the decrease in the treatment group was obviously superior to that in the control group, the difference being significant between the two groups (P <0.05). Conclusion Plum-blossom needling combined with Deanxit shows better effect than Deanxit for the treatment of depression or anxiety in chronic tension headache patients.

The factors influencing the integration of MOOC and information literacy education in academic libraries were divided into objective factor, subjective factor and social factor according to their connotation and significance. Certain measures for their integration were proposed, such as strengthening network construction, perfecting literacy education systems, and carrying out systemic training.Integration of MOOC and information literacy education in academic libraries can improve information service, promote wide application of MOOC and information literacy ed-ucation in academic libraries.

Objective: To explore the relationship between the cognitive style of field dependence-independence and self-supporting levels in primary school students.Methods: One hundred and twenty grade 4 to 6 primary school students were examined by the Self-supporting Behavior Questionnaire for 6 to 12 Years Old Children,and then the subjects were divided into two groups,of which the high-self-supporting group includes the top 27% according to the questionnaire total score,and the low-self-supporting group includes the bottom 27%.Both the groups were tested by the Embedded Figures Test.Results: ①The high self-supporting group get significantly higher score in the Embedded Figures Test than the low self-supporting group;and the field-independence group get significantly higher scores in the scholastic self-supporting and self-determination subscales of the Self-supporting Behavior Questionnaire than the field-dependence group;②the score of Embedded Figures Test was significantly correlated with the scores of scholastic self-supporting,self-supporting in daily life,self-determination,self-action,responsibility undertaking,and the total score of the Self-supporting Behavior Questionnaire.Conclusion: Children's self-supporting levels were related to their cognitive style,namely,the high self-supporting children were inclined to field-independence cognitive style,and vice versa.

Objective:To explore the relationship between Parental Rearing Behavior and self-supporting levels in pri-mary school students.Methods:①79 grade 5 to 6 primary school students were examined by the Self-supporting Behav-ior Questionnaire for 6 to 12 Years Old Children and EMBU scale;②And then the subjects were divided into two groups,of which the high-self-supporting group includes the top 27% according to the questionnaire total score,and the low-self-supporting group includes the bottom 27%.Results:①Compared with the low self-supporting group,the high self-supporting group get significantly higher score of the emotional warmth and understanding of father and mother;compared with the high self-supporting group,the low self-supporting group get significantly higher score of refusal of mother,rejec-tion and punishment of father and mother;②Self-supporting of children was negatively correlated with refusal of mother,rejection and punishment of father and mother;positively with emotional warmth and understanding of father and mother.Conclusion:Children's self-supporting levels were related to parental style.

Objective:To explore the relationship between self-supporting levels and mental locus of control(internalexternal locus of control)in primary school students.Methods:193 grade 5 to 6 primary school students were examined by the Self-supporting Behavior Questionnaire for 6 to 12 Years Old Children and Multidimentional Measure of Children’s Perception of Control(MMCPC);and then the subjects were divided into two groups,of which the high-self-supporting group includes the top 27%according to the self-supporting behavior questionnaire total score,and the low-self- supporting group includes the bottom 27%.Results:①The high self-supporting group get significantly lower score in unknown control and powerful others’control subscales than the low self-supporting group,and get significantly higher score in internal locus of control than the low self-supporting group;②Except social self-supporting,the other dimentions in Self-supporting Behavior Questionnaire were negatively correlated with unknown control and powerful others’control; were positively correlated with the internal control.Conclusion:Children’s self-supporting level were closely related to mental locus of control.the high self-supporting children were inclined to the internal control,and vice versa.

Objective: To explore the relationship between self-supporting levels and the early parent-child attachment in primary school students. Methods: 193 grade 5 to 6 primary school students were examined by the Self-supporting Behavior Questionnaire for 6 to 12 Years Old Children and Adult Attachment Questionnaire (AAQ 3.1); and then the subjects were divided into two groups, of which the high-self-supporting group included the top 27% according to the self-supporting behavior questionnaire total score, and the low-self-supporting group included the bottom 27%. Results: ①The low-self-supporting group got significantly higher score in mother negative loving, father negative loving, mother rejecting, father rejecting, mother anger and father anger subscales than the highself-supporting group; ②The children’s self-supporting behaviors were negatively correlated with mother negative loving, father negative loving, mother rejecting, father rejecting, mother anger and father anger. Conclusion: Children’s self-supporting levels were closely related to the early parent-child attachment. The refusal, rejection, negative loving, and anger of parents might play a passive role in the development of children’s self-supporting behavior.

Objective:To explore the relationship between the self-supporting levels and self-concept in primary school students.Methods:193 grade 5 to 6 primary school students were examined by the Self-supporting Behavior Questionnaire for 6 to 12 Years Old Children and Tennessee Self-concept Scale,and then the subjects were divided into two groups,of which the high-self-supporting group includes the top 27% according to the questionnaire total score,and the low-self-supporting group includes the bottom 27%.Results:①The high self-supporting group get significantly higher score in all subscales of Tennessee Self-concept Scale than the low self-supporting group;②All subscales of Tennessee Self-concept Scale were significantly positively correlated with all dimentions of Self-supporting Behavior Questionnaire.Conclusion:Children's self-supporting behaviors were closely related to self-concept.The higher level of self-supporting,the more positive their self-concept.

OBJECTIVE: To discuss marketing combination program strategy of online drug store in China.METHODS: It was analyzed from aspects of product strategy,price strategy,channel strategy and promotion strategy.RESULTS & CONCLUSIONS: For product strategy of online drug store,great importance should be attached to the rational selection of products;the product strategy should manifest its advantage of low price;the distribution channel strategy should be conducive to the close rate and the promotion means in promoting strategy should be diversified.

OBJECTIVE:To provide reference for the selection of marketing strategy for post-marketing drugs.METHODS:?2 test was employed to analyze the number of 4 marketing strategies(flank attack strategy,guerrilla strategy,defensive strategy and attack strategy)used for the 100 well-known drug brands(74 from domestic versus 26 from abroad),meanwhile the regular marketing strategies for the domestic vs.foreign drug enterprises and for the OTC drugs vs.prescribed drugs were analyzed.RESULTS & CONCLUSIONS:There were significant differences between domestic and foreign pharmaceutical enterprises in the use of guerrilla strategy and attack strategy,20 of the domestic brands(27.03%)vs.none of the foreign brands used guerrilla strategy(0.00%),however,the foreign enterprises prefer attack strategy than domestic enterprises(14 foreign brands(53.85%)vs.13 domestic ones(17.57%)).There were no significant differences between domestic and foreign brands in the application of flank attack strategy and defensive strategy,the same is true between OTC and prescription drugs in the four strategies mentioned above.