Objective To investigate the effct of PTTG gene on the growth and sensitivity to 5-FU of cholangiocarcinoma cell.Methods The pcDNA3.1-PTTGas that contained full-length antisense PTTG,and pcDNA3.1(+) was transfected into the cholangiocarcinoma cell line QBC939.We successfully established and identified cell sublines tQBC939(PTTG-),tQBC939(pcDNA3.1).Cell growth curve was mapped out,cell proliferation rate was assessed by MTT assay and cell cycle percentage was counted by flow cytometry.After treatment with 5-FU,cell survival rate was assessed by MTT assay and IC50 was calculated.Flow cytometry and Hoechst staining were used to demonstrate discrepancy of cell apoptosis rates.Results Compared with other two groups,tQBC939(PTTG-) grew more rapidly and had a higher proportion of cells in S phase,whereas less cells in G2/M phase(P

OBJECTIVE: To investigate the effects of purslane herb aquenous extracts (PHAS) on the stress ability of aging mice induced by D-galactose. METHODS: We observed the survival time to hypoxia and heat survival rate of the mice treated with different doses of PHAS and vitamin E. The contents of lipofuscin and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) and catalase (CAT) in the brain and liver of the mice were tested. RESULTS: As compared with vitamin E, three doses of PHAS (1.6, 0.8 and 0.4 ml/d) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate, and the 0.8 ml/d PHAS had the best effect. In the group of 0.8 ml/d PHAS, the activity of SOD and CAT decreased less, and the contents of lipofuscin and MDA decreased significantly. The effect of vitamin E was not as good as the PHAS. CONCLUSION: PHAS can prevent the stress ability of the aging mice. One of its mechanisms may be increasing the activity of SOD and CAT, hence decreasing the damage of the oxidation products to the body.

Objective To analyze the significance of higher vocational nursing students participating in opening professional social practice,and to explore the effective way of combining social practice with professional learning and clinical practice.Methods After completing the opening professional social practice in summer,the reflection diaries of 30 nursing students were analyzed and the concerns were refined.Questionnaire survey about self assessment of comprehensive effect of the practice of nursing students were conducted.Results Nursing operation and soft skills were mostly concerned by nursing students' in opening professional social practice.Vocational learning,occupation of responsibility,and the occupation ability were improved by practical activities.Conclusions Nursing students who participate in opening professional social practice before clinical work,can re-understand the professional learning,adapt and integrate themselves into clinical practice through the perception of clinical nursing work.

The construction of smart library needs the support of good,multiple and effective information resources system.The necessities for information resources system in smart library were outlined in aspects of starting from the development of library holdings,sharing and open access of resources,deep revealing of resources by making full use of the discovery service system,strengthening management and assessment of resources.

Smart library is an advanced stage in the development of library and service is the key content in aca-demic library.The service model of smart library was described from the angle of information service, cloud service and Library 3 .0 with the connotation of smart library and the service function of academic library as the starting points.in an attemp to provide reference for academic libraries to establisht their target of smart library construction.

Theancientandmodernprocessingmethods,activeingredientresearch,andqualitycomparisonbeforeandafterthe processing of medicinal leech were reviewed in the paper. Combined with the results of anticoagulation test on Whitmania pigra Whit-man, the article drew a conclusion that different varieties of medicinal leech had different active ingredients of anticoagulation, Whitma-nia pigra Whitman had active ingredients of anticoagulation with promising heat stability, and the processing technology should be dealt with according to the clinic use.

To cement the 5 years’ staged achievement of new medical reform so as finally achieve the objective of medical reform, public hospital would become the aim of reform in the following stage. On one hand , canceling “using drug to supple health care” in public hospitals has came into the crucial stage; on the other hand, the government needed to increase financial input and ensure the implementation of security fund. What was the relation between these two reforms? Therefore , based on the survey of county-level public hospitals in G Province, relationship among government compensation method, hospital public profit operation mechanism and profit-driven operation mechanism were analyzed; besides, different financial insurance mode had different operation efficiency. Because the construction of hospital expense under public profit operation mechanism was more reasonable , the required government insurance fund was less, which only accounted 32.9%of the required fund under the profit-driven operation mechanism. Therefore, the policy suggestions that government need to “buy the mechanism” and “be brave to implement ‘subtraction reform’” were proposed.

Objective To investigate the curative effects of partial internal sphincterotomy in the posterior midline and lateral position in treatment of chronic anal fissure. Methods One hundred and fifty cases chronic anal fissure patients with stageⅡ or Ⅲ were randomly divided into observation group(80 cases)and control group(70 cases),who were hospitalized in the Shunyi District Hospital of Beijing from Dec. 2009 to Oct. 2012. The patients of two group were treatment by internal sphincterotomy either in the posterior midline or lateral position respectively. The efficacy,healing time,complications and recurrence of the two groups were recorded and compared. Results There were 76 cases with cure,6 cases with improvement and 2 with ineffective effect in the observer group. There were 49 cases with cure,12 cased with improvement and 9 cases with ineffective effect in control group. The difference of the efficacy was statistically significant( P =0. 025). Wound healing time of observation group and control group were(9. 9 ± 0. 8)d and(17. 1 ± 0. 9)d,and the difference was significant( P =0. 048 ). The incidence of anal exudates in observer group was lower than the control group(2. 5%(2/80)vs. 24. 3%(17/70);P=0. 032). The incidence of recurrence in observer group was 5. 0%(4/80),lower than the control group(24. 3%(17/70),P=0. 046). The degree of the postoperative pain,voiding dysfunction and wound edema in observer group were lower than the other group( P =0. 025, 0. 025,0. 049). Conclusion The effect of internal sphincterotomy in the lateral position in treatment of chronic anal fissure is better with quickly repair,fewer complications.

OBJECTIVE: To provide reference for rational use of drugs. METHODS: 200 ADR cases induced by TCM injection from Huizhou were analyzed retrospectively. RESULTS: Of total 200 ADR cases, 133 cases were male, which was significantly higher than females in 67 cases(P

Expression of PRL receptor mRNA in Jurkat D1.1 cell line was confirmed by RT PCR. CD154 expression of Jurkat cells was significantly increased when these cells were incubated with PHA and recombinant human PRL, suggesting that binding of PRL to its receptor promotes CD154 expression which plays a role in immune regulation.