Objective To understand the clinical distribution and drug resistance situation of112 strains of Acinetobacter Bau-mannii isolated from the patients′sputum samples in cerebral surgery ICU in order to provide the basis for the rational use of anti-bacterial drugs in clinical therapy.Methods 112 strains of Acinetobacter Baumannii were performed the retrospective analysis.The VITEK2-Compact automatic microbiological analyzer was adopted to conduct the bacterial identification and the drug susceptibility test and the K-B method was combined as the supplement control.The Whonet 5.6 software was used for conducting the statistical analysis.Results Among 112 strains of Acinetobacter baumannii,75 strains(67.0%)were multi-drug resistant,8 strains(7.1%) were pan-resistant.The antimicrobial susceptibility tests showed that the resistant rates of Acinetobacter baumannii to mezlocillin, piperacillin and nitrofurantoin reached up to 100%.Acinetobacter Baumannii had the lower resistant rate to polymyxin B (2.7%), the resistant rates to minomycin and ceperazone/sulbactam were 4.5% and 10.7% respectively.Conclusion The drug resistance phenomena of Acinetobacter Baumannii in the cerebral surgery department ICU is serious.Minomycin,polymyxin B and ceperazone/sulbactam have the better antibacterial activities to clinically isolated Acinetobacter Baumannii and may be the alternative drugs for therapy.Clinic should strengthen the monitoring and rationally use the antibacterial drugs.

Objective To investigate the impacts of Xuebijing injection on systemic inflammatory response and immune function of patients with acute exacerbation of severe chronic obstructive pulmonary disease (AECOPD). Methods A prospective randomized controlled trial (RCT) was conducted. Forty patients with severe AECOPD were divided into control group and Xuebijing group in accordance with the random number table, each group 20 cases. Both groups were treated by routine conventional basic therapy of severe AECOPD including anti-infection, phlegm-expelling formula, bronchodilators and mechanical ventilation, etc, while in Xuebijing group, intravenous drip of Xuebijing (100 mg, twice a day for 5 days) was additionally used. The changes of data of arterial blood gas analysis, blood routine examination, C-reactive protein (CRP), procalcitonin (PCT), immune function, and acute physiology and chronic health evaluation system Ⅱ (APACHE Ⅱ) score before and after therapy were observed and compared between the two groups. The length of stay in hospital, duration of mechanical ventilation, and prognosis were also compared between the two groups. Besides, according to the difference in APACHE Ⅱ score, all the patients were divided into APACHEⅡscore≥15 score group (18 cases) and APACHEⅡscore<15 score group (22 cases), and the immune function were compared between the two groups. Results ①The immune function was descended and disordered in patients with severe AECOPD. Compared with the < 15 score group, the expressions of CD45+, CD3+, CD4+, CD4+/CD8+ ratio, B-lymphocyte, natural killer cell (NK cell) in ≥ 15 score group were significantly lowered [CD45+(×106/L):663.92±100.61 vs. 1 289.92±169.38, CD3+(×106/L):342.05±108.93 vs. 882.37±172.56, CD4+(×106/L):205.96±63.97 vs. 486.24±108.64, CD4+/CD8+ratio:0.76±0.49 vs. 1.32±0.57, B-lymphocyte (×106/L):124.77±32.72 vs. 166.06±48.02, NK cells (×106/L): 186.47±39.57 vs. 243.51±44.72, all P < 0.05]. There was no statistically significant difference in the expression of CD8+ between the ≥ 15 score group and < 15 score group (P > 0.05). ② Compared with those before therapy, the pH value, oxygenation index, arterial partial pressure of carbon dioxide (PaCO2), white blood cell count (WBC), CRP, PCT were significantly improved after therapy in both control and Xuebijing groups. Compared with those in the control group, WBC, CRP, PCT were significantly lowered in Xuebijing group [WBC (×109/L): 10.29±3.83 vs. 12.69±3.42, CRP (mg/L): 9.06±4.19 vs. 15.26±4.22, PCT (ng/L): 0.18±0.21 vs. 0.42±0.24, all P < 0.05]. There were no statistically significant differences in pH value, oxygenation index and PaCO2 between two groups (all P>0.05). The degrees of improvement of CD45+, CD3+, CD4+, CD8+ and CD4+/CD8+ ratio were more remarkable in Xuebijing group after treatment than those in control group [CD45+ (×106/L): 1 079.38±153.86 vs. 1 015.63±157.11, CD3+ (×106/L): 652.05±100.05 vs. 596.81±106.85, CD4+ (×106/L): 358.92±67.53 vs. 329.99±72.61, CD8+ (×106/L): 205.73±35.19 vs. 230.41±39.74, CD4+/CD8+ratio: 2.16±0.63 vs. 1.52±0.54, all P < 0.05]. The B-lymphocyte and NK cell before treatment and after treatment showed no statistical significant differences between the two groups (all P > 0.05). Compared with the control group, the duration of mechanical ventilation (hours: 56.25±22.87 vs. 69.45±26.59) and the length of stay in hospital (days: 11.00±0.74 vs. 14.00±2.06) were shortened, and APACHE Ⅱ score was significantly lowered in Xuebijing group (8.21±2.97 vs. 12.08±3.12, P < 0.05). The numbers of multiple organ failure and dead patients in Xuebijing group were less than those of control group, but no statistical significant differences were found (both P > 0.05). Conclusion Xuebijing injection for treatment of patients with severe AECOPD can ameliorate inflammatory response, improve immune function, shorten the duration of mechanical ventilation and the length of stay in hospital, and decrease the risk of death.

Adult ; Burns ; etiology ; Humans ; Male ; Occupational Exposure ; Phosphorus ; adverse effects ; Young Adult

Background The cytochrome P450-dependent monooxygenase 2D6 (CYP2D6) ac- tivity can he estimated by investigating the metabolism of model drugs or by genotyping the most com- mon CYP2D6 alleles. The CYP2D6 allele frequencies are well investigated in Caucasians whereas no comprehensive allele analysis has been done in mainland Chinese. The aim of the study was to investi- gate C2938T, G1846T, G1846A and C188T gene mutation in mainland Chinese. Methods Three tet- ra-primer PCR assays and one allele specific amplification (ASA)-PCR assay were developed to easily genotype C2938T, G1846T, G1846A and C188T mutation in CYP2D6 allele in 223 cases of mainland Chinese. Results The most frequent allele was * 10 (C188T) with a prevalence of 51.3%, followed by * 2 allele (C2938, 15. 5%) in mainland Chinese. The distribution frequence of a novel variant CYP2D6 * 14B was 2.0%. This novel * 14B allele was different from * 14 allele by the absence of the C188T substitution and by the additional G1749C substitution. Conclusion This comprehensive study of CYP2D6 allele in mainland Chinese shows some genetic diversity compared to Chinese from other regions. Genotyping is a useful method in applying drug individuation.

To explore the of occupational therapy for the cerebral palsied children was introduced. The assessment areas of occupational therapy and evaluational instruments commonly used. The last part gives the concrete treatment methods that occupational therapy usually adopts in practice, specially in the fields of training and developing the grass motor skills of the upper limbs, the fine motor skills of the hands and ADL skills of the children with cerebral palsy were also stated.

It's known that ICU patients is in critical condition and might deteriorate rapidly,so it's important for them to get appropriate nutritional support in order to recover.We must make proper nutrition treatment plan for patients according to their different nutritional condition and potential risk.Nutritional risk screening can not only assess the nutritional status of children,is more likely to predict its potential nutritional risk,to guide nutritional support treatment plan,and ultimately improve the prognosis.Looking out for comprehensive,simple,fast,and easy nutritional risk screening tools as soon as possible and appropriate nutrition risk screening is significantly for growth and development as well as prognosis of ICU patients.

The definition of cancerous toxin and the relationships between cancerous toxin and other factors including the etiopathogenesis, pathogenesis and treatment of malignant tumor were discussed in this paper. The author suggests that the cancerous toxin is engendered by the disequilibrium of human body, which is the foundation of tumorigenesis. Cancerous toxin is the main pathogenic factor or one of the main pathogenic factors during the development of malignant tumor, hence it is also the key point for understanding and treating malignant tumor.

Syndrome research is a challenging field in the research on traditional Chinese medicine and there is no breakthrough at all in this field as yet, although some progresses from various aspects have been achieved in recent years. The definition of syndrome based on the conceptions of traditional Chinese medicine is basically clear, but the qualitative macro-criteria for syndrome are not standardized and the research methods for quantification of such criteria are not unified. These hindered the processing of the modernization of traditional Chinese medicine to a great extent; therefore new research methods for syndrome using techniques of modern sciences should be further investigated. During the research of syndrome, it is suggested that: (1) the research should be turned to complex syndrome from the fundamental simple syndrome gradually, on the basis of certain diseases in western medicine; (2) animal experiment would be of no significance before quite a successful research performed in human body; (3) the results of the research of syndrome should be in agreement with the life science as well as the entire natural science.

There exist some problems in the present cancer treatment, such as: (1) prevalence of excessive treatment; (2) lack of long-term research plan; (3) lack of reasonable comprehensive treatment; (4) lack of operative individualized treatment; (5) the superiority of traditional Chinese medicine (TCM) was undervalued and underused. Integrative medicine should play an important role in the prevention and treatment of cancer. Further research should be carried out in the following aspects: (1) enrich and improve the theoretical knowledge of TCM on cancer; (2) enhance the research on rationalization and standardization of cancer treatment program of integrative medicine; (3) enhance the research on criterion of therapeutical effect of integrative medicine in treating cancer; (4) enhance the research on prevention and treatment of postoperative recurrence and metastasis of cancer by integrative medicine.

OBJECTIVE: To investigate the effects of purslane herb aquenous extracts (PHAS) on the stress ability of aging mice induced by D-galactose. METHODS: We observed the survival time to hypoxia and heat survival rate of the mice treated with different doses of PHAS and vitamin E. The contents of lipofuscin and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) and catalase (CAT) in the brain and liver of the mice were tested. RESULTS: As compared with vitamin E, three doses of PHAS (1.6, 0.8 and 0.4 ml/d) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate, and the 0.8 ml/d PHAS had the best effect. In the group of 0.8 ml/d PHAS, the activity of SOD and CAT decreased less, and the contents of lipofuscin and MDA decreased significantly. The effect of vitamin E was not as good as the PHAS. CONCLUSION: PHAS can prevent the stress ability of the aging mice. One of its mechanisms may be increasing the activity of SOD and CAT, hence decreasing the damage of the oxidation products to the body.