Objective:To investigate the relationship between flurbiprofen axetil injection and atrial fibrillation in the elderly pa-tients after cardiac surgery and underlying mechanism. Methods: A retrospective research method was applied, and the medical re-cords of 62 elderly patients with cardiac surgery were chosen and divided into the experimental group and the control group according to the different medication. After the heart surgery, fentanyl was used in the control group, and flurbiprofen was applied in the experimen-tal group. Before and after the treatment, the serum C-reactive protein levels, the incidence of postoperative atrial fibrillation, postop-erative atrial fibrillation duration, length of stay, incidence of cardiovascular disease in 6 months and adverse reactions were observed and compared between the two groups. Results:After the treatment, the serum CRP levels in the experimental group were significantly lower than those before the treatment and those in the control group after the treatment (P<0. 05 or P<0. 01). The incidence of post-operative atrial fibrillation in the experimental group was significantly lower than that in the control group (P<0. 05). The duration of postoperative atrial fibrillation in the experimental group was significantly shorter than that in the control group (P<0. 05). The differ-ences in the postoperative hospital stay, the incidence of cardiovascular disease in six months and the incidence of adverse reactions were not statistically significant between the two groups (P>0. 05). Conclusion: Flurbiprofen axetil injection used after cardiac sur-gery can reduce the incidence of atrial fibrillation, which shows important clinical significance.

Objective To investigate the pollution situation of ventilation systems of centralized air-conditioners in public areas and to present the data for hygiene management. Methods 20 ventilating systems of air-conditioners from six 3-star or above hotels in Nanning were selected randomly and the hygienic inspection on these samples was performed including the indexes of PM10, aerobic bacterial count, aerobic molds count, dust capacity of inner-wind duct surface and microbial as well as the legionella in cooling tower water. Results The total count of aerobic bacterial in breeze of air-conditioner was rather serious, the over-standard rate was 66.7%. The over-standard rate of PM10 was 16.7%. Dust content of inner-wind duct surface was rather high with the average value of 46.33 g/m2 and the over-standard rate was 83.3%, the over-standard rate of total count of aerobic molds in it was 100%, the highest value was 145.3 times of the standard limit. The over-standard rate of total bacterial count in inner-wind duct surface was 16.7%. The legionella pneumophila was detected in one of the six samples. Conclusion The cleaning and disinfection of ventilation system of centralized air-conditioners in star grade hotels is not thorough in Nanning and the pollution of microbial is rather serious. so the hygienic management must be strengthened.

Objective To establish the advanced lung cancer model with effusion in the pleural cavity by implanting the lung adenocarcinoma A549 cells into the lung and pleural cavity of mice.Methods The Balb /c-nu female mice were divided into three groups and injected with the subculturing lung adenocarcinoma A549 cells to make three models in the subcutaneous (group A),the lungs (group B) and the lungs and pleural cavities (group c).The survival time,weightconsume of feeds,signs and symptoms of cachexia,growth of lung cancer and pleural effusion were observed.Results The median survival time in group C and group B were obviously reduced compared with group A.The range discrete in the group A was higher than group B and group C.There was no significant difference between group B and group C in the average survival time.The effective survival time was significant different between group B and group C.There were differences among three groups in the signs and symptoms of cachexia,the weight and consume of feeds.In 4 weeks,the rate of growing effusion in the pleural cavity was 93.3％ in group C,and only 20.0％ in group B.Conclusions The mice modal implanted the lung adenocarcinoma A549 cells into the lung aud pleural cavity of mice in the same time can be as the adwanced lung cancer model with effusion in the pleural cavity.

Objective To investigate the significance of cellular inhibitor of apoptosis proteins 2 (cIAP2) expression in the patients with hepatitis B and non-hepatitis B related hepatocellular carcinoma (HCC).Methods The medical record data and tissue samples in the patients with HCC resection operation were collected.Expression of cIAP2 in HCC cancer lesion,adjacent tissues and cancer-distant tissues was detected by immunohistochemical staining and Western blotting.Results In the cancer lesion,paracancerous tissues and cancer-distant tissues of the two groups,the cIAP2 expression amount was decreased in turn.But in the non-hepatitis B related HCC group,the cIAP2 expression in the cancer-distant tissues was significantly lower than that in the HCC cancer lesion and paracancerous tissues,while in the hepatitis B related HCC group,the cIAP2 expression amounts had no significant difference between the caner-distant tissues and paracancerous tissues,while lower than that in the cancer lesion.Conclusion cIAP2 is one of important mechanisms causing hepatic B related HCC and can serve as a therapeutic target point for inhibiting HCC development and eliminating hepatitis B virus.

OBJECTIVE:To explore the effects of extracts from Siegesbeckiae herba (HS) on doxorubicin (DOX)-induced chronic myocardial injury in mice. METHODS:48 mice were randomly divided into 3 groups. Mice in blank control(Con)group received distilled water once every day,ig,and normal saline(2 mL/100 g)once every day,ip,for 8 weeks;mice in DOX mice received distilled water(2 mL/100 g)once every day,ig,and DOX(3 mg/kg)once every week,ip,for 8 weeks;mice in DOX+HS group received HS(340 mg/kg)once every day,ig,and DOX(3 mg/kg)once every week,ip,for 8 weeks. After administra-tion,body mass,heart coefficient,cardiac function changes,serum biochemical index levels [alanine aminotransferase(ALT),as-partate aminotransferase (AST), creatine phosphokinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH)and total cholesterol(TC)] of mice were determined. RESULTS:Compared with Con group,body mass of mice in DOX group was decreased(P<0.01);heart coefficient was increased(P<0.05);heart rate slowed down,R-wave was increased(P<0.05 or P<0.01);serum biochemical indexed were increased,there was significant difference in AST(P<0.01). Compared with DOX group,heart coefficient of mice in DOX+HS group was decreased (P<0.01);heart rate was increased (P<0.01);serum biochemical indexes were decreased,there was significant differences in CK,LDH,TC(P<0.01). CONCLUSIONS:HS has cer-tain protective effects on DOX-induced chronic myocardial injury in mice.

Pulmonary embolism (PE) refers to the endogenous or exogenous emboli blocking pulmonary trunk or branches, causing clinical and pathophysiological syndrome of pulmonary circulation disorder, the incidence rate is high. Sometimes PE patients were lack of specific symptoms and signs, or without any symptoms, which often result in misdiagnosis, un-timely diagnosis, and the delay of treatment. A PE case with syncope, vomiting and shock, which was proved to be pulmonary artery trunk and branch wide embolism later, was presented so as to improve the understanding of the disease.

Objective: To provide data support for AIDS prevention and control by investigating HIV testing among technician school students in Guangzhou. Methods: A total of 1 112 students were investigated with a questionnaire about AIDS knowledge, attitude, sexual behavior and HIV testing through a stratified cluster random sampling method in October 2018. A Logistic regression analysis was conducted for influencing factors of students’ HIV testing. Results: The HIV testing rate of technician school student was 10.3%. The HIV testing rate (12.1%-24.3%) of male, under 18 years of age, with monthly living expenses over 3 000 yuan and with poor family atmosphere was higher than that of the corresponding group (6.6%-8.4%), the difference were statistically significant (χ2=12.07,7.25,10.73,9.77, P<0.05). Multivariate Logistic regression analysis showed that <18 years of age, willingness to participate in relevant AIDS prevention activities, homosexuality, “support for multiple sexual partners” and having sexual behavior were associated with more HIV testing(P<0.05). Conclusion The incidence of sexual behavior of students in technician school is high while the rate of HIV testing is low, especially for male and male homosexuals.The health and education departments should strengthen students’ AIDS counseling and testing services and raise students’ awareness of AIDS risk in order to improve the coverage of students’ HIV testing.

Objective To observe the expressions of serum insulin-like growth factor (IGF)- Ⅰ ,Ⅱ and IGF binding protein (IGFBP) 3, 5 and to explore the clinical significances in patients with clear cell carcinoma of kidney. Methods Enzyme-linked immunosorbent assay (ELISA) methods were adopted to examine serum expressions of IGF-Ⅰ , Ⅱ and IGFBP 3, 5 in 40 cases with clear cell carcinoma of kidney (renal carcinoma group) and 16 cases with hydronephrosis (control group) from May 2007 to December 2009. Results IGF- Ⅰ , Ⅱ and IGFBP 3,5 in renal carcinoma showed higher expressions before operation (985. 7 μg/L, 1154.0 μg/L,46.6 μg/L and 9.6 μg/L, respectively)than after operation (431.4 μg/L, 632.6 μg/L, 26.7 μg/L, and 6.7 μg/L, respectively, all P＜0. 05 ～0.01). There were no significant differences in those indexes between pre- and post- operation in control group (P＞ 0. 05). Conclusions There are high expressions of serum IGF-Ⅰ , Ⅱ and IGFBP 3, 5 in renal carcinoma patients, and IGF- Ⅱ has clinical significance in diagnosis.

Objective To investigate the common initial clinical presentations of primary Sj(o)gren's syndrome (pSS) with pulmonary complications,and to explore the differences between patients with extraglandular manifestations at disease onset (EGM) and those with glandular manifestations at disease onset (GM).Methods A total of 1 341 hospitalized SS patients from 2003 to 2012 were retrospectively reviewed.Of them,102 hospitalized patients with pSS'associated lung disease were analyzed and included.Case control study was performed to explore the differences between the EGM group and the GM group.Results Fifty-one percent of patients were presented with EGM at onset,with significantly shorter disease duration [36 (12,156) m vs 102 (48,159) m,x2=-2.41,P=0.016].Although the mean diagnose time was similiar,only 4％ of the EGM group could be confirmed the pSS diagnose at onset,which was significantly less frequently than that of the GM group (34％,22=15.29,P＜0.01).Case control study revealed that hyperglobulinemia,elevated RF titers and anti-SSA and/or anti-SSB test positive were less predominant in the EGM group [IgG 16(12,21) g/L vs 21 (15,28) g/L,x2=-2.15,P=0.032;22 (20,171) U/ml vs 104 (20,238) U/ml,x2=-l.98,P=0.048;33％ vs 72％,x2=15.78,P＜0.01].The predicted value of TLC and FVC were lower [(87±23)％ vs (97±20)％,x2=-1.96,P=0.050;(8±28)％ vs (100±27)％,x2=-1.70,P=0.089] and HRCT score was higher in EMG group [12(88,15) vs 8(5,13),x2=-1.82,P=0.070].Conclusion EMG at onset is the common initial manifestation of pSS'associated lung involvement.Pulmonary complication is more progressively and severe than those with MG at onset.Anti'SSA positive,elevated RF titer and hyperglobulinemia are not predominant for patients with EMG at onset.

Objective:To evaluate the efficacy and safety of focused radiofrequency in facial wrinkle and laxity.Methods:Seventeen female patients (47.71±1.56 years of age) were involved in our study during January to June 2018, treated with focused radiofrequency on the whole face. The treatments were performed monthly for three consecutive months. Their photographs were taken before treatment and one week after the last treatment, and then the wrinkle severity rating scale (WSRS) and global aesthetic improvement scale (GAIS) were evaulated.Results:After three times＇ treatment, the wrinkle was improved, WSRS of 1 week after the last treatment (2.71±0.47) was much lower than the baseline (3.00±0.79) ( P<0.05). 88.24% patients were satisfied with the improvement. There was no severe adverse effects during this whole procedure. Conclusions:Focused radiofrequency therapy for facial wrinkle and laxity is effective and safe.