Traumatic brain injury(TBI) in humans inclines to cause sensorimotor and cognitive dysfunction.In order to evaluate sensory,motor and cognitive function post injury,many researchers at home and abroad have established some animal models in TBI and methods of behavior assessment.The goal of this review is to describe some tests employed to assess neurofunctional outcome after TBI.

Objective:To compare the clinical effects of gargle fluid containing scutellaria baicalensis with chlorhexidine in the treatment of chronic gingivitis and to observe TNF-α expression in gingival crevicular fluid(GCF) before and after treatment.Methods:90 cases of chronic gingivitis were divided into 3 groups (n =10) based on random number table and treated by scaling and mouth wash with normal saline(group A),chlorhexidine(group B) and scutellaria baicalensis(group C) respectively.PI and GI were measured,TNF-α level in GCF was examined after initial therapy,1 week,2 week and 1 month after treatment.Results:Comprehensive analysis of the whole treatment process showed that there was difference of PI among 3 groups(F =3.506,P =0.034).PI in group B was lower than that in group A and C (P ＜ 0.05;A vs C,P ＞ 0.05);there was difference of GI among the 3 groups (F =6.359,P =0.003).GI in group A was higher than that in group B and C(P ＜0.05;B vs C,P ＞0.05),there was difference of TNF-α concentration in GCF among the 3 groups(F =3.780,P =0.027);TNF-α in group A was higher than that in group B and C(P ＜0.05,B vs C,P ＞0.05).Conclusion:Radix scutellariae and chlorhexidine gargle fluid are equally effective in the treatment of chronic gigivitis.

PurposeTo investigate the mechanism of BM210955 (ibandronate, a new bisphosphonate drug manufactured in China)in the prevention of bone loss in vitro. MethodsThe osteoclasts isolated from the long bone of 10-day-old Rabbit were cultured on glass and bone slices in different concentration of BM210955. TRAP(tartrate resistant acid phosphonase) staining for osteoclast and TB( toluidine blue)staining for resorption lacunae were used on bone slices and Fluorescence (orange acridine) staining for apoptosis cell on glass slices was used;Multinucleated( three and more nuclei) TRAP positive cell and Apoptosis cell and Pits were counted. ResultsThe BM210955 decreased the multinucleated cell number by 73% in 10-8 mol/ L;The inhibitory rate of pit formed was correlate to concentration as 31.58%, 76.32% and 87.99% to 10-12, 10-10 and 10-8 mol/L; The apoptosis induction was shown in above 10-8 mol/L, and the apoptosis rate was 62% in 10-4 mol/L. Conclusions Induction of osteoclast apoptosis and decrease of the cell number and inhibition of resorptive ability were the major mechanism for bone loss prevention effect of BM210955 in vitro.

Objective By exploring the relationship between the gene polymorphism of tumor related gene CD44 and the occurrence of breast cancer,the pathogenic gene or genetic predisposing gene of breast cancer was studied so as to provide theoretical basis and technical support for the screening,diagnosis and treatment of breast cancer.Methods Samples from 265 cases of breast cancer and 290 healthy female were collected. Multiple polymerase chain reaction (PCR) amplification and two generation sequencing were used to analyze the genetic polymorphism of 4 loci of CD44 rs11607862,rs13347,rs7116432 and rs8193.T he size and metasta-sis of 235 patients were compared.Results There was no significant difference in the expression of four sitesloci in breast cancer patients.However,in the analysis of cancer patients,the GG genotype of rs13347 lo-cus was lower than that of CC/CG type,and the lymph node metastasis rate was lower.Conclusion There is a certain correlation between the rs13347 loci of CD44 and the metastasis of breast cancer.The correlation be-tween the genetic polymorphisms of 3 loci of rs11607862,rs7116432 and rs8193 and the development of breast cancer needs more clinical samples and further research.

No abstract available.
Psoriasis*

BACKGROUND: Imidacloprid has been commonly used as a pesticide for crop protection and acts as nicotinic acetylcholine receptor agonists. Little information about the relationship between imidacloprid and allergy is available. OBJECTIVE: This study aims to examine the effects of imidacoprid on IgE-mediated mast cell activation. METHODS: The rat basophilic leukemia cell line RBL-2H3 (RBL-2H3 cells) were treated with 10⁻³ – 10⁻¹² mol/L imidacloprid, followed by measuring the mediator production, influx of Ca²⁺ in IgE-activated RBL-2H3 cells, and the possible effects of imidacoprid on anti-dinitrophenyl IgE-induced passive cutaneous anaphylaxis (PCA). RESULTS: It was shown that imidacoprid suppressed the production of histamine, β-hexosaminidase, leukotriene C4, interleukin-6, tumor necrosis factor-α, and Ca²⁺ mobilization in IgE-activated RBL-2H3 cells and decreased vascular extravasation in IgE-induced PCA. CONCLUSION: It is the first time to show that imidacloprid suppressed the activation of RBL-2H3 cells.
Animals ; Basophils ; Cell Degranulation ; Cell Line ; Crop Protection ; Histamine ; Hypersensitivity ; Interleukin-6 ; Leukemia ; Leukotriene C4 ; Mast Cells ; Necrosis ; Passive Cutaneous Anaphylaxis ; Rats ; Receptors, Nicotinic

Objective:To explore the diagnostic value and clinical significance of total volume of quadratus femoris muscle (TQFMV), ischial angle, femoral neck angle (FNV) measured by magnetic resonance imaging (MRI) combined with eccentric distance and lesser trochanter height measured by multi-slice spiral CT (MSCT) in the diagnosis of ischiofemoral impingement (IFI) syndrome.Methods:A total of 82 patients with IFI in Beijing Huairou Hospital from October 2017 to July 2020 were selected as the observation group. In addition, 82 healthy patients who underwent MRI and MSCT were collected as the control group. The general data, MRI and MSCT parameters of the two groups were compared, and IFI influencing factors were analyzed by logistic regression. The correlation between MRI and MSCT parameters and clinical manifestations and the correlation between MRI and MSCT parameters were analyzed. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of MRI and MSCT parameters for IFI.Results:There were statistically significant difference between the two groups of gender, age, MRI parameters (TQFMV, ischial angle, FNV), and MSCT parameters (eccentricity, lesser trochanter height) (all P<0.05). Logistic regression analysis showed that gender, age, MRI parameters (TQFMV, ischial angle, FNV), MSCT parameters (eccentricity, lesser trochanter height ) were all influencing factors of IFI (all P<0.05). MRI parameters (TQFMV, ischial angle, FNV), MSCT parameters (eccentricity, lesser trochanter height) were all related to quadratus femoris muscle (QFM) edema, fat infiltration and pain degree in IFI patients (all P<0.05). The MRI parameter TQFMV of IFI patients was positively correlated with the MSCT parameter eccentricity and lesser trochanter height, while the ischial angle and FNV were negatively correlated with the MSCT parameter eccentricity and lesser trochanter height (all P<0.05). The AUC of MRI parameters (TQFMV, ischial angle, FNV) and MSCT parameters (eccentricity, lesser trochanter height) in the diagnosis of IFI were high, especially the highest in combined diagnosis, reaching 0.859. Conclusions:MRI parameters TQFMV, ischial angle, FNV and MSCT parameters, eccentricity and lesser trochanter height are related to the clinical manifestations of IFI patients. Combined detection of them can improve the diagnostic value of IFI and avoid missed diagnosis and misdiagnosis.