Purpose To study immunohistochemical quality of prostatic basal cell(PBC). MethodsTherty-seven of basal cell of benign prostatic hypertrophy (BPH) were studied using routine HE stavningand En Vision immunohistochemical methods. Results The positive rate of 34βE12 in PBC were 100 %;but all of the PBC were negative with Vim and SMA; the positive expression of PsAP PSA in PBC were35% ;expression of ck in the PBC was 71% and the positive rate of S-100 in PBC was onty 13.5%.Conclusions The resuts suggest that high molecular weight keratin 34βE12 is a specific marker to PBC;PBC has ability to adenoid epithelia differentiation of prostate and hyperplasia itself. PBC play an importantrole in growth, hyperplasia and disease of prostate. The PBC unlike myoepithelia, the ability to myoepitheliadifferentiation is to go a step further controversial opinion.

Purpose:To investigate the expression of PCNA、p53、C erbB 2 in colorectal carcinoma and its relationship with host immune reactions. Methods:The expression of PCNA、p53、C erbB 2 was detected by Envision immunohistochemical method in 42 colorectal carcinoma, 35 pericarcinomatous and 10 normal coloerctal mucosa respectively. Host immune reactions (pericarcinomatous fibrosis, mononuclear infiltration and lymph nodes reaction) were also observed. Results:(1) The positive expression of PCNA、p53、C erbB 2 in colorectal carcinoma was significantly higher than in corresponding pericarcinomatous mucosa and normal colorectal mucosa respectively( P 0.05). (3) GH was more evident than PH in colorectal carcinoma and GH had reverse relationship with lymph node metastasis( P

Background Oxidative stress has been implicated in the pathogenesis of angiotensin Ⅱ(Ang Ⅱ)related hypertension.Superoxide anion,produced by NAD(P)H oxidase,plays important roles in hypertension and its complications.Ang Ⅱ infusion increases cerebral NAD(P)H oxidase activity,which is inhibited by angiotensin Ⅱ receptor blocker(ARB)candesartan and NAD(P)H oxidase inhibitor.However,the underlying mechanism of ARB in preventing hypertensive stroke is not elucidated clearly.Objective To investigate the effects of candesartan on the expression of cerebral NAD(P)H oxidase mRNA in salt-loaded stroke-prone spontaneously hypertensive rats(SHRsp).Methods Twelve-week-old salt-loaded SHRsp were treated with candesartan [1.0 mg/(kg?d)],trichlormethiazide [TCM,1.6 mg/(kg?d)] or vehicle(n=12 in each)for 2 weeks.Age-matched salt-loaded WKY rats were served as control(n=12).Blood pressure was measured every week.After two weeks,cerebrums were harvested and 24 h urine was collected.Urinary albumin was examined by ELISA.Cerebral cortex NAD(P)H oxidase subunits(p22phox,p47phox and gp91phox)mRNA expression were assayed by real-time PCR.Results The systolic blood pressure was increased significantly in salt-load SHRsp.Candesartan and TCM reduced SBP to the similar level.Urinary albumin excretion and cerebral cortex NAD(P)H oxidase subunits were markedly higher in salt-loaded SHRsp than those in salt-loaded WKY rats.Incidence of stroke was significantly reduced in candesartan treated group as compared with TCM treated SHRsp(P

OBJECTIVEThis study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. METHODS In this study, we investigated the antimicrobial activity of decapeptide against Streptococcus mutans (S. mutans), Streptococcus sobrinus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gordonii, Actinomyces viscosus, Actinomyces naeslundii, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Saccharomyces albicans in vitro using the agar diffusion method and broth dilution method. Furthermore, a time-kill kinetic study of decapeptide against S. mutans was performed.

RESULTSThe results showed that decapeptide exhibited antimicrobial activity against various oral bacteria and fungi. The minimum inhibitory concentration (MIC) of main cariogenic bacteria ranged from 62.5 μg · mL(-1) to 125 μg · mL(-1), and the MIC of periodontopathogenic bacteria tested ranged from 250 μg · mL(-1) to 1,000 μg · mL(-1). Among the bacteria tested, decapeptide had a strong inhibitory effect on cariogenic S. mutans. Results of the time-kill kinetic studies showed that decapeptide reduced the viable counts of S. mutans by more than one order of magnitude after 20 min of incubation, and thoroughly killed S. mutans after 30 min. No viable cells could be detected after 24 h of incubation.

CONCLUSIONThis study suggest that decapeptide might have potential clinical application in treating dental caries by killing S. mutans within dental plaque.

Aggregatibacter actinomycetemcomitans ; Anti-Bacterial Agents ; Anti-Infective Agents ; Bacteria ; Dental Caries ; Dental Plaque ; Kinetics ; Microbial Sensitivity Tests ; Mouth ; microbiology ; Porphyromonas gingivalis ; Streptococcus mutans

Objective To study the effects of low dose sodium arsenite to human bone marrow mesenchymal stem cells(hBMSCs) differentiation during establishing of arsenic-resistant cell model. Methods hBMSCs were prepared in conventional method and continuously exposed to 1μmol/L sodium arsenite for ≥12weeks inv vitro. Forty-eight hours acute arsenite toxicity test was drived to assay if the cells acquired arsenic-resistance. The proliferation capacity of CAsE-hBMSCs was observed by the rate of colony formation.The expression of Oct-4 in CAsE-hBMSCs was assayed by RT-PCR and immunocytochemistry. The expression of ABCG2 in CAsE-hBMSCs was analyzed by RT-PCR. Results hBMSCs continuously exposed to 1μmol/L sodium asenite for ≥12 weeks exhibited dramatic resistance to acute arsenite toxicity. The LC 50 for acute arsenite exposure in CAsE-hBMSCs was 35.59μmol/L versus 18.04μmol/L in control cells. Compared to control cells, the CAsE-hBMSCs didn't show malignant proliferation ability. Expression of Oct-4 gene was positive in 4th, 18th passage hBMSCs and the hBMSCs induced by arsenite for 4 weeks but negative in CAsE-hBMSCs. The expression of Oct-4 protein was positive and weakly positive in 4th passage hBMSCs and CAsE-hBMSCs respectively, and the positive granules of Oct-4 distributed in cytoplasm. The expression of ABCG2 gene in CAsE-hBMSCs was obviously lower than that in control cells ( P <0.001). Conclusion Human bone marrow mesenchymal stem cells could be induced to differentiation by low dose sodium arsenite.

Objective To observe the effect of MG132 on the expression of extracellular regulated kinase 1/2 (ERK1/2) and connective tissue growth factor (CTGF) in rat peritoneal mesothelial cells (RPMCs) induced by high glucose.Methods RPMCs were isolated,cultured and passaged by trypsin,then identified.The second generation of cultured RPMCs were used in the experiment.RPMCs were divided into normal control group,high glucose (1.5％,2.5％,4.25％) for 24 hours,high glucose (2.5％) for 0,12,24,48 hours,incubated with MG132 (0.5,1,2 μmol/L) for half an hour and then with high glucose (2.5％) for 24 hours.ERK1/2 protein was detected by Western blotting,and CTGF protein in supernatant was detected by ELISA.Results Compared with the control group,the expression of p-ERK1/2 was significantly increased in the groups stimulated by high glucose (P ＜0.01),reached the peak at 24th hour (P ＜ 0.01),and then the expression decreased at 48th hour,but still was higher than that in the normal control group (P ＜ 0.01).CTGF protein expression of RPMCs induced by high glucose increased,in time-and dose-dependent manner (P ＜ 0.05).MG132 could significantly decrease the expression of ERK1/2 and CTGF induced by high glucose (P＜0.05).Conclusions MG132 can decrease the expression of p-ERK1/2 and CTGF in RPMCs induced by high glucose.The ubiquitin proteasome pathway participates in the development of peritoneal fibrosis,and blocking the way may contribute to the prevention of peritoneal fibrosis.

BACKGROUND:Endothelial progenitor cells are recruited into local vascular injury under the injury-induced stimulation, and then differentiate into mature endothelial cells that are thereby involved in angiogenesis and endothelial repair. OBJECTIVE:To investigate whether endothelial progenitor cells can al eviate renal injury and improve renal function of ischemia/reperfusion injury (I/R) rats. METHODS:Peripheral blood samples extracted from Sprague-Dawley rats were used to isolate and culture endothelial progenitor cells using density gradient centrifugation. Twenty-two male Sprague-Dawley rats were randomized to three groups:I/R group, normal control group, and endothelial progenitor cells group. In the I/R and endothelial progenitor cells groups, the right kidney was removed and the renal artery and vein of the left kidney were occluded for 40 minutes to establish I/R models in the rats, and then endothelial progenitor cells (5×109/L, total y 1 mL) or solvent was transplanted via the artery of the left kidney into the left kidney. In the normal control group, the experimental procedure was same as that in the I/R group except for occlusion of the artery and vein of the left kidney. Renal and blood samples from three groups were col ected at day 1 after operation. Peripheral blood CD34 and vascular endoethelial growth factor receptor 2 expressions were determined using flow cytometry and immunofluorescence methods, serum creatinine and urea nitrogen were tested, and immunohistochemistry observation was used for CD34 observation. RESULTS AND CONCLUSION:Compared with the normal control group, serum creatinine and urea nitrogen levels were significantly increased, and tubulointerstitial CD34 expression was decreased in the I/R group (P<0.05). Endothelial progenitor cells treatment largely decreased the levels of serum creatinine and urea nitrogen, and increased CD34 expression (P<0.05). These findings indicate that transplantation of endothelial progenitor cells contributes to renal protection in I/R rats.

BACKGROUND:Studies have shown that ankle-foot orthosis can increase the feedback on the input information from receptors in the skin of the foot and leg to improve the ankle joint position sense, and promote brain function reorganization.
OBJECTIVE:To systematical y evaluate the effect of ankle-foot orthosis on the improvement of walking in hemiplegic patients.
METHODS:The Chinese Biomedical Literature Database, CNKI, WanFang Data and VIP database were searched for reports of randomized control ed trials of ankle-foot orthosis to improve walking ability in hemiplegic patients, from the date of establishment of each database to June 2013. The randomized control ed trials which met the criteria were included for the Meta-analysis.
RESULTS AND CONCLUSION:A total of 9 randomized control ed trials involving 456 patients were included. Meta-analysis showed that, compared with conventional treatment and drug therapy, ankle foot orthosis via the continuous treatment shows certain advantages to improve lower extremity motor function in hemiplegic patients, life skil s and 10-meter maximum walking speed. Due to a limited number of included documents, the remaining indicators such as walking speed, stride difference and balance function were only for appropriate descriptive analysis. The results suggested that, by improving abnormal gait, walking speed, stride frequency, gait cycle, space asymmetry, ankle muscle spasms and balancing, the ankle-foot orthosis could achieve the goal of improving walking function. Ankle-foot orthoses could not be confirmed to exert the role in the fol owing indicators, including time asymmetry, double support phase prolongation and stride length. This evidence shows that ankle-foot orthoses in hemiplegic patients may promote recovery of motor function of the lower limbs and activities of daily living to a certain extent, but the more high-quality, multi-center randomized control ed trials with large samples are necessary.

Background:The efficacy of standard triple therapy for eradication of Helicobacter pylori(Hp)is decreasing in recent years,and bismuth-containing quadruple regimen becomes the first-line therapy for Hp infection. However,the application of bismuth has some limitations. Aims:To investigate the efficacy of proton pump inhibitor(PPI)-based standard triple therapy combined with teprenone for Hp eradication. Methods:A total of 155 Hp-infected chronic gastritis patients were enrolled from Jan. 2013 to Dec. 2014 at Aerospace Center Hospital,and were randomly assigned into standard triple therapy group,bismuth-containing quadruple therapy group and teprenone-containing quadruple therapy group. Patients in standard triple therapy group were given rabeprazole sodium 20 mg + amoxicillin and clavulanate potassium 914 mg +clarithromycin 500 mg bid for 10 days. Patients in bismuth-containing quadruple therapy group were given standard triple therapy combined with bismuth potassium citrate 0. 6 g bid for 10 days. Patients in teprenone-containing quadruple therapy group were given standard triple therapy combined with teprenone 50 mg bid for 10 days. Symptoms of patients were evaluated before and after eradication therapy. Four weeks after the end of therapy,13 C-urea breath test( UBT)was reexamined to assess the status of Hp infection. Results:One hundred and fifty-two patients completed the treatment course. By ITT analysis,the eradication rates of standard triple therapy group,bismuth-containing quadruple therapy group and teprenone-containing quadruple therapy group were 69. 8%(37 / 53),86. 5% (45 / 52)and 88. 0% (44 / 50), respectively;by PP analysis,the eradication rates of the three groups were 71. 2%(37 / 52),88. 2% (45 / 51)and 89. 8%(44 / 49),respectively. Eradication rates analyzed by ITT and PP in bismuth-containing quadruple therapy group and teprenone-containing quadruple therapy group were significantly higher than those in standard triple therapy group(P <0. 05),but no statistically significant difference was found between these two groups( P > 0. 05). Relieving rates of abdominal pain,abdominal distention and belching in bismuth-containing quadruple therapy group and teprenone-containing quadruple therapy group were higher than those in standard triple therapy group,but statistically significant difference was only found in relieving of abdominal pain between teprenone-containing quadruple therapy group and standard triple therapy group(88. 9% vs. 70. 3% ,P < 0. 05). Adverse effects included transient and mild nausea,diarrhea and black stool, etc. Conclusions:Teprenone-containing quadruple regimen are effective for Hp eradication and symptoms relieving with no severe adverse effects,the efficacy is comparable with bismuth-containing quadruple regimen.

Objective To explore the effect of action research based on group learning in hospice care module for nursing students. Methods 82 students were divided into cooperative learning group (40) and conventional teaching group (42). The cognition and attitude of good death questionnaire was used to evaluate teaching effect before and after teaching of hospice care. After teaching, self-feedback and self-feedback questionnaire were made to evaluate the effect of cooperative learning group. SPSS 17 was used to make comparison of data between groups and within groups, and t test, chi square test or Fisher exact probability 2 were also used . Results There were no difference between two groups of nurses before teaching. The students' cognitive level and attitude in cooperative learning group increased significantly, especially their cognitive level of physiological needs (t=5.398, P=0.001), cognition and attitude of death education in intervention group was higher than control group (t=2.992, P=0.004; t=3.661, P=0.001). Although 95% of the nursing students of the cooperative learning group thought group cooperative studying could improve their interests and cultivated their comprehensive abilities, only 50% could accept this kind of studying methods . Conclusions Action research based on group cooperative studying can improve students' cognitive level and attitude of good death, and it is also good for cultivating their ability of au-tonomous learning, innovation, cooperation, and meanwhile, educational action research is a good way to improve both teaching effect and research ability of teacher staffs, but in practice, students' acceptance of group learning needs to be improved.