Objective To explore the effect of psychological intervention combined with drug multimodal treatment on analgesic effect and stress reaction in Department of orthopedics.Methods80 cases of Department of orthopedics patients in Ruian City People's Hospital from March 2016 to March 2017 were selected and randomly divided into control group and observation group, 40 cases in each group.The control group was treated with drugs only, and the observation group was treated with psychological intervention combined with multi multimodal.The analgesic effect and stress response of the 2 groups were observed.ResultsThe satisfaction rate in observation group were significantly higher than the control group.There was no significant difference in heart rate, diastolic blood pressure and systolic blood pressure between two groups before intervention, after group intervention, the changs in observation group were relatively small, and the difference was statistically significant(P<0.05).Visual acupuncture scores were observed at different time points,the pain in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05).ConclusionMultimodal therapy for analgesia in the Department of orthopedics and found psychological intervention combined with stress reaction the drug, compared with simple drug intervention, can play a good analgesic effect, at the same time to reduce the patient's stress response, so it is worthy of reference.

Objective To investigate the pathological features and significance of m yocardial contraction band necrosis in sudden cardiac death. Methods Using HE and PTA Hstaining, the distribution sites and pathological features of m yocardial contraction band necrosis were observed. The data were analyzed accord-ing to the extent of necrosis. Results The locations, pathological features and the extent of necrosis were sim ilar in different sudden death cases. The locations were related with sites of m yocardial dam age. The papillary m uscles of left ventricular were m ost occurred, followed by the anterior wall of left ventricular. In the sudden death cases caused by stress cardiom yopathy, the extent of m yocardial contraction band necrosis was lighter than the others. Most cases were predom inantly level 1, the others were level 2. Conclusion Used as the diagnosis basis of acute m yocardial ischem ia, the m yocardial contraction band necrosis has im portant significance to determ ination of death.

Individual stature estim ation is one of the m ost im portant contents of forensic anthropology. C urrently, it has been used that the regression equations established by the data collected by direct m ea-surem ent or radiological techniques in a certain group of lim bs, irregular bones, and anatom ic landm arks. D ue to the im pact of population m obility, hum an physical im provem ent, racial and geographic differ-ences, estim ation of individual stature should be a regular study. T his paper review s the different m ethods of stature estim ation, briefly describes the advantages and disadvantages of each m ethod, and prospects a new research direction.

OBJECTIVE To analyze the contamination situation in the oxygen aspiration facility and the measure for infection control.METHODS The first half of the year 2006 was determined as a traditional disinfection supervision group,the second half of the year 2006 was an improvement disinfection group,65 and 62 unused damping bottles in traditional disinfection and improvement disinfection groups were detected.Sixty three and 62 samples of continuously used damping liquid in the two disinfection groups were detected daily.The damping liquid detection of d1 and d6 in above two groups were compared each with other.The detection result was statistically analyzed.RESULTS The statistical analysis of the unused damping bottle qualification ratio between the two groups was with significant differences,P

Purpose Blast lung injury is severe, and is the main causes of death in explosion. This paper aims to explore the imaging features of blast lung injury, and to provide reference for clinical diagnosis and care. Materials and Methods The clinical data of 37 patients with primary blast lung injury stricken in the 8.2 Kunshan explosion in 2014 were retrospectively studied, with regard to the degree of burn, area, auxiliary emergency treatment and medical history. The imaging manifestations of X-ray and/or CT were also analyzed. Results ① The X-ray and/or CT findings of chest showed that 6 cases (16.2%) presented no obvious abnormality of lung parenchyma, 14 cases (37.8%) manifested increase of lung markings and spot-like clouding opacity, 6 cases (16.2%) presented ground glass opacity, 7 cases (18.9%) manifested patchy shadow, 4 cases (10.8%) showed diffuse consolidation. 6 cases (16.2%) with pleural effusion, 6 cases (16.2%) complicated with pneumothorax and hydropneumothorax, 4 cases (10.8%) with enlarged podoid and pericardial effusion, 2 cases (5.4%) with mediastinal hematoma, 9 cases (24.3%) with rib fracture. The positive rate of X-ray film inspection was 72.2% (13/18), yet that of CT reached 92.9% (13/14). ② Five patients who had not been seen obvious abnormal in emergency lung examination appeared mottling shadows or flake fuzzy shadows in the examination on the second day; 9 cases with aggravated symptoms and infection signs displayed relieved signs, expanded or changed primary lesions. Conclusion Imaging examination is an important tool in the diagnosis of primary blast lung injury. CT is superior to X-ray film on the detection of lesions; therefore, CT examination on chest should be carried out as soon as possible if conditions are allowed.

Objective IFN-? is a pleiotropic cytokine with potent immunomodulatory effects and antiviral activity. To study the mechanism of IFN-? clearing duck hepatitis B virus (DHBV) in ducks, it is essential to establish a method to quantify expression of DuIFN-? in immune response. In the present study,a semi-quantitative competitive RT-PCR was developed to quantify expression of duck IFN-?(DuIFN-?) mRNA by PBMCs. Methods Based on ?-actin consensus sequence, fishing the ?-actin gene as house keeping gene from duck PBMC by RT-PCR. A competitive internal control was constructed and the competitive RT-PCR system could be used to quantify the transcription of DuIFN-? mRNA. Results After duck PBMCs were stimulated in vitro with PHA, the peak of DuIFN-? expression was at 24-36h. Then RT-PCR method was applied to detect DuIFN-? mRNA transcription by PBMCs from DHBV infected ducks immunized with DuIFN-? plasmid plus DNA vaccine or DNA vaccine alone. Results showed that expression of DuIFN-? in ducks co-immunized with DuIFN-? plasmid were higher than other groups immunized without DuIFN-? plasmid as adjuvant. Conclusions The results indicated that DuIFN-? gene could be a useful adjuvant to develop vaccines. The semi-quantitation of DuIFN-? mRNA by competitive RT-PCR provides the basis for future study of the mechanism of IFN-? in duck hepatitis B virus persistent infection.

Objective To compare the effectiveness and safety of radiofrenquency ablation(RFA) with other therapeutic methods for patients with early hepatocelluar carcinoma(HCC). Methods Randomized clinical trials(RCTs)which compared the efficacy or safety of RFA with other therapeutic methods for primary hepatocellular carcinoma in Cochrane library,EMBASE,PubMed,OVID and CBM were searched.Trials were considered of high quality if methodological quality score was 3 or more according to Jadad standard.Statistical heterogeneity between trials was evaluated bv STATA 9.0 and considered to exist when P＜0.1.Heterogeneity of the included articles was tested and used to select proper effective model for calculation.Sensitivity analysis was performed and publication bias was investigated through visual inspection of funnel plots and Egger regression model.Results Six RCTs including 862 cases were analyzed.As compared with other therapeutic approaches,RFA significandy increased 3-year overall survival rate and reduced local recurrence rate of early hepatocelluar carcinoma:the total OR were 2.06(95%CI being 1.54-2.77,P=0.000)and 0.40(95%CI being 0.28-0.57,P=0.000)respectively.As compared with other therapeutic approaches,the total OR of new HCC recurrence rates,extrahepatic metastasis rate and major complications in patients with HCC treated with RFA were 0.92 (95%CI being 0.68-1.24),0.98(95%CI being 0.30-3.22),1.35(95%CI being 0.49-3.77)respectively,showing no significant differences(P＞0.05).Inspection of the funnel plots for all outcome measures did not reveal evidence of publication bias(P=0.670,0.160,0.884,0.087,0.317,respectively,by Egger regression model).Conclusions RFA is superior to other treatment methods with respect to local recurrence and 3 year overall survival in early HCC and is the preferred tberapeutic method for small HCC because it is minimally invasive,simple and convenient.

Background Application of available technology and objective indexes are very important for the early diagnosis,monitoring and therapeutic evaluation of primary open angle glaucoma (POAG).Many studies have determined retinal damage in structure and function in POAG.However,the study on the association of structural damage and functional abnormality in early POAG is still lack.Objective This study was to evaluate the relationship between structural and functional changes of retina in early stage of POAG.Methods A prospectively pilot study was performed under the approval of Ethic Committee of Shenzhen Eye Hospital from January 2011 to June 2013.Based on Helsinki Declaration,written informed consent was obtained from subject prior to entering the cohort.Ninety-five eyes of 95 POAG patients were included as the study group,and 41 eyes of 41 non-glaucoma subjects were enrolled at the same period as controls.The structural parameters of retinas were measured using RTVue-100 OCT and Cirrus HD-OCT respectively,including macular ganglion cell complex (GCC)-Avg thickness and peripapillary retinal neural fibril layer (RNFL)-Avg thickness;and the functional parameters of retinas were obtained by Humphrey visual filed analyzer and RETI scan 3.15 system respectively,including MD of visual field and PhNR of flash electroretinogram (F-ERG).The associations between the GCC or RNFL thickness and M D or amplitude of PhNR were evaluated by linear and curvilinear regression models.Results The MD,GCC-Avg,RNFL-Avg and PhNR amplitude were (-0.68±1.72)dB,(97.17± 4.82)μm,(102.51±8.74) μm and (49.61±11.01)μV respectively in the control subjects,and those in the POAG patients were (-10.82±9.87) dB,(75.07±12.29) μm,(69.09±12.96) μm and (28.38± 11.52) μV,showing significant differences between them (t =6.549,11.118,-15.061,9.956,all at P=0.001).The curvilinear regression model appeared to better describe the relationship between GCC thickness and MD (R2 =0.595,F=97.089,P＜0.001) ;while a linear regression model seemed to be better fit for the relationship between GCC thickness and amplitude of PhNR (R2=0.437,F=103.413,P＜0.001).RNFL thickness analysis showed the similar regression models with MD and amplitude of PhNR as GCC thickness,but R2 values were higher between the RNFL thickness and MD (R2 =0.606,F =101.666,P＜0.001) or amplitude of PhNR (R2 =0.454,F=54.983,P＜0.001).Conclusions Both GCC thickness and RNFL thickness show a curvilinear relationship with MD and a linear relationship with amplitude of PhNR.Goodness-of-fit of RNFL thickness is superior to GCC thickness.

Objective T o explore the occurrence and the differences of clinical m anifestations of organic personality disorder w ith varying degrees of craniocerebral traum a. Methods A ccording to the Interna-tional C lassification of D iseases-10, 396 subjects w ith craniocerebral traum a caused by traffic accidents w ere diagnosed, and the degrees of craniocerebral traum a w ere graded. T he personality characteristics of all patients w ere evaluated using the sim plified N euroticism E xtraversion O penness Five-Factor Inventory (N E O-FFI). Results T he occurrence rate of organic personality disorder w as 34.6% w hile it w as 34.9%and 49.5% in the patients w ith m oderate and severe craniocerebral traum a, respectively, w hich signifi-cantly higher than that in the patients (18.7% ) of m ild craniocerebral traum a (P<0.05). C om pared w ith the patients w ithout personality disorder, the neuroticism , extraversion and agreeableness scores all show ed significantly differences (P<0.05) in the patients of m ild craniocerebral traum a w ith personality disorder; the neuroticism , extraversion, agreeableness and conscientiousness scores show ed significantly differences (P<0.05) in the patients of m oderate and severe craniocerebral traum a w ith personality disor-der. T he agreeableness and conscientiousness scores in the patients of m oderate and severe craniocerebral traum a w ith personality disorder w ere significantly low er than that of m ild craniocerebral traum a, and the patients of severe craniocerebral traum a had a low er score in extraversion than in the patients of m ild craniocerebral traum a. Conclusion T he severity of craniocerebral traum a is closely related to the in-cidence of organic personality disorder, and it also affects the clinical features of the latter, w hich pro-vides a certain significance and help for forensic psychiatric assessm ent.

Objective To observe the effects of inhalation salbutamol combined with psychological intervention on the treatment of patients with severe asthma, and evaluate its clinical curative effect. Methods 162 cases with severe asthma were selected from February 2014 to February 2017 in hospital of traditional Chinese medicine in Zhoushan as the research object, which were randomly divided into the control group and the study group with 81 cases in each group. All patients were given nebulized salbutamol, the control group in the perioperative were given routine nursing intervention, at this basis, the study group were given targeted psychological nursing. The effect in the two groups was compared. Results The total effective rate in the study group was 91.36％ (74/81) was significantly better than that in the control group 76.54％ (72/81), the difference was statistically significant (P<0.05). After treatment, cough, shortness of breath, wheezing, chest tightness, lung wheezing were improved significantly , and all the symptoms in the study group was obviously better than those in the control group (P<0.05). Conclusion Aerosol inhalation of salbutamol combined with psychological intervention on the treatment of severe asthma patients has significant curative effect, which can significantly improve the clinical treatment effect, improve the clinical symptoms in a timely manner, and has the value of application and promotion.