Acupuncture Therapy ; Female ; Humans ; Polycystic Ovary Syndrome ; therapy

Objective To study the IgG antibodies on red cell surface in ABO and Rh-incompatible infants and to evaluate its predictive value for hemolysis. Methods From Nov. 2001 to Jul. 2002,50 full term newborn babies with ABO and Rh incompatibility hemolysis were admitted .They were divided into two groups according to the direct Coombs test(DCT): 38 cases with positive result and 12 cases were negative. Another 30 normal newborns were chosen as control group. Erythrocyte associated IgG,A and M were measured by a flow cytometry (FCM). Results the mean level of concentration of IgG antibodies in positive DCT group was (12.64?16.83)%,while (4.54?2.54)% in negative DCT group ( P 427.5 ?mol/L,IgG was elevated with the increased TSB level. There was a close relationship between the concentration of TSB and IgG ( r =0.678). Conclusion Measurement of erythrocyte surface antigen IgG assists the diagnosis of maternal-fetal incompatible hemolysis,especially when with a positive direct Coombs test result.

Objective To investigate the pathological diagnosis of high-grade intraepithelial neoplasia in gastric biopsy.MethodsOne hundred and forty-three cases diagnosed by gastric biopsy as high-grade intraepithelial neoplasia were analyzed by light microscopy,and were compared with the pathological findings of surgical specimens in 60 cases and a second biopsy in 13 cases.Results Among the sixty cases treated surgically following the gastric biopsies,no carcinoma was found in 6,while the other 54 were diagnosed as gastric adenocarcinoma,27 in advanced stage and 27 in early stage,respectively.Fifteen cases were well-differentiated,24 moderately-differentiated,12 poorly-differentiated and 3 were of mucinous type.Four of the 13 cases with a second biopsy were diagnosed as well-differentiated adenocarcinoma,8 low-grade intraepithelial neoplasia and 1 chronic inflammation.The colorectal adenomas-like changes were morphologically found in 6 surgically-treated cases without carcinoma,5 of whom mucous muscles were not found by biopsy.Fibroblastic reactions were found in 9 cases,all of which were diagnosed as adenocarcinoma in surgical specimens.Conclusion Ninety percent of the cases who are diagnosed as high-grade intraepithelial neoplasia by biopsy may have already been complicated with adenocarcinoma.The location and depth of the specimen in gastric biopsy have a considerable influence on pathological diagnosis.The fibroblastic reaction may be considered as an important marker for gastric adenocarcinoma in pathological diagnosis of gastric biopsy.

Objective To investigate the incidence and related risk factors of female stress urinary incontinence(SUI) in a county of Chengdu. Methods The women were interviewed with questionnaires,which were designed to the modified standard of the Advisory Committee of Urinary Incontinence of Asia-pacific Region. Results The total number of the women investigated was 563,while the number of the valid questionnaires was 512,with the efficient rate 98.5%.Of these women,46.1%(236/512) had urinary incontinence,21.9%(112/512) had simplex SUI,4.7%(24/512) had simplex urgent urinary incontinence and 19.5%(100/512) had complex urinary incontinence.The incidence of SUI(simplex SUI plus complex urinary incontinence) was 41.4%(212/512).The incidence of SUI was associated with factors such as age,pregnancies,drinking and education level,and the severity was associated with age,body mass index and education level.Conclusion The incidence of SUI in this county of Chengdu is relatively higher.Heath education is very important for the female health.

Sleep disorders are commonly occurred among patients with Parkinson's disease,such as difficulties in the initiation of sleep,fragmented sleep,sleep behavior disorder and excessive daytime sleepiness.The mechanism of sleep disorders associated with Parkinson's disease is not clear,which may be associated with the injury of brain stem,nuclei of median raphe,nuclei fasciculi solitarii,thalamencephalon and the changes of neurotransmitters as dopamine,hypocretin(orexin) and melatonin.This article gives an overview of the mechanism of sleep disorders associated with Parkinson's disease.

ObjectiveTo investigate sleep disorder of patients with depression and find out the regularity.Methods78 depression cases in the east-region of Shenyang were evaluated by Pittsburgh Sleep Quality Index (PSQI) questionnaire and mental health symptom checklist-90 (SCL-90). The results were contrasted with normal control group.Results63 depression cases (80%) had sleep disorder (PSQI scores≥8) and were significantly higher than normal control group. The PSQI evaluation showed that sleep quality, enter-sleep time, sleep efficiency, sleep disorder, hypnotige drugs, function of daytime and total scores of PSQI of the depression group were significantly higher than that of normal control group (P<0.05～0.01). The SCL-90 test showed that scores of somatic, obsessive, depressive, anxiety, phobia, paranoid and mental-illness, etc. and total quota of bad-sleep quality group were significantly higher than that of the fine-sleep quality group (P<0.05～0.01).ConclusionDepression patients have significantly descent in sleep quality, latter is often closely related to the mental psychotic expressiveness of somatic, obsession, depression, anxiety, phobia, etc.

Objective To establish an animal model of knee osteoarthritis (OA) , and to explore the relationship between cartilage degeneration and the expression of IL-1β and MMP-1. Methods A total of 16 healthy male rabbits (32 hind knee joints) were used. The experimental OA model was established in their left knees by intraarticular injection of0.2 ml of 4% papain solution and 0.1 ml of 0.03 mol/L L-cysteine solution. The right knees were used as the controls and were treated with intraarticular injection of 0.3 ml of normal saline solution. General and histological scoring, and IL-1β and MMP-1 expression intensity were compared between the 2 groups. Results The degree of cartilage degeneration in the left knees was higher than in the right knees as observed by naked eye and using an electron microscope. The left knees scored significantly higher than the right ones with Mankin's scoring system. There was significantly greater expression of IL-1β and MMP-1 in the chondrocytes of the left knees. Conclusions lntraarticular injection of papain and L-cysteine can induce experimental knee osteoarthritis in rabbits. The expression of IL-1β and MMP-I is significantly related with carti-lage degeneration.

Objective To investigate the effects of uhrashortwave therapy and passive motion on experimen-tal osteoarthritis caused by immobilization of the joint. Methods Twenty healthy male rabbits had their left knee joints fixed in extension for 4 weeks. They were randomly divided into 4 groups : a control group which did not receive any treatment, an uhrashortwave therapy group, a passive motion group, and an ultrashortwave therapy plus passive motion group, and treated accordingly for 4 weeks. The range of motion of the joint before and after treatment was compared in every group. At the end of the 4th week, all the rabbits were sacrificed, and the cartilage at the condylus medialis femoris was sampled and observed with toluidine blue staining, haematoxylin-eosin staining. Its gross appearance was noted and it was also analyzed using immunohistochemical techniques. Results ①Passive range of motion (PROM): there were no significant differences among the 4 groups before the treatment. Uhrashortwave therapy per se did not yield significant therapeutic effects in terms of PROM as compared to the controls, However, passive motion alone and in conjunction with ultrashortwave therapy brought about significant improvement of PROM when compared against the control group. The most significant change was seen in the integrated group, followed by the passive motion group. ②The histological scoring system : Mankin's scoring system showed significant differences a-mong all 4 groups. The highest value was the control group, which was followed by the ultrashortwave therapy group, the passive motion group and the integrated group. ③The positive expression rate of inducible nitric oxide synthase: there were significant differences among all 4 groups. The control group had the highest values, followed by the ultra-shortwave treated group, the passive motion treated group and the integrated group. Conclusions Ultrashortwave therapy, passive motion therapy and integrated therapy combining ultrashortwave therapy with passive motion can all can reduce and prevent the cataplasia of articular cartilage. Integrated therapy is the best treatment method, followed by passive motion therapy, and then uhrashortwave therapy.

Objective To evaluate the diagnostic value of tissue Doppler imaging (TDI) in classification of fetal arrhythmias, and conclude characters of each type of arrhythmias by analyzing wave forms of TDI. Methods Fifty-five fetuses suffered arrhythmia were included in study group. By comparing the results with control standard(M and PW), the sensitivity, specificity and reliability of TDI in diagnosis of fetal arrhythmias was calculated. At last, the character of waves by TDI of each type of arrhythmia was concluded. Fetal kinetocardiogram was made to identify the origin of arrhythmias. All cases were asked to perform electrocardiography and echocardiography examination after birth. Results The sensitivity, specificity and reliability of TDI was 98%, 100% and 100%, respectively. The character of atrioventricular waves and kinetocardiogram of 8 types fetal arrhythmias were conclude. Conclusions TDI has great value in classification of fetal arrhythmias.