Background Many researches have reported the high proliferation ability of pterygium tissue.However,the origin of cells in pterygium tissue and their role in pathogenesis and recurrence of pterygium are below understand up to now.Objective This study is to explore the role of bone marrow derived stem cells and progenitor cells in the pathogenesis of pterygium.Methods Informed consent was obtained from 12 patients with the pterygium stretching the edge of the pupil prior to this trail.Pterygium specimen was obtained from 8 patients with primary pterygium and 4 patients with recurrent pterygium during the operation.The normal conjunctival tissue was obtained from the location of 10 mm from the pterygium in the operation eye.The expression levels of marker of the primitive haematopoietic progenitors(AC133),marker of the haematopoietic progenitor cells and endothelium (CD_(34)),marker of haematopoietic and stromal progenitor cells (C KIT ),and differentiation antigen of bone marrow fibroblast cells and nonhaematopoietic progenitor cells 1 (STRO 1) were detected by using immunohistochemistry.Results Various progenitor cell markers originated from bone marrow,including CD_(34),AC133,C KIT,STRO 1,were positively expressed in pterygium specimen rather than normal conjunctival specimen mainly in the basal epithelium or stroma of pterygia tissue.Majority of the positive cells were seen in the head of pterygium.C KIT positive cells were observed mainly in the basal epithelium and stroma of pterygium.The morphology of positive cells varied upon different distribution.Fibroblast like and spindle shaped positive cells were seen in the stroma layer of pterygium,and round or ovoid shaped cells were in basal epithelial layer.The distribution and shape of positive cells for AC133 and CD_(34) were similar to ones of C KIT but numbers of cells were varied between them.C KIT,AC133 and CD_(34) positive cells were coexpressed in basal epithelium and vascular endothelium.Expression of STRO 1 was found in the stroma layer not in the basal epithelium of pterygium.Similar distribution,numbers and shape of positive cells were seen between recurrent and primary pterygium.Conclusion The cells with stem cells markers and progenitor cells markers originated from bone marrow may play a role in the pathogenesis of pterygium.

OBJECTIVE:To observe the efficacy and safety of Huanglian shangqing soft capsule in the adjuvant treatment of in-flammation around the implant. METHODS:40 patients(72 implants)with inflammation around the implant were randomly divid-ed into control group (20 cases) and observation group (20 cases). Control group received full mouth supragingival scaling tech-nique,subgingival curettage,root planing,occlusal adjustment,loose teeth fixation or removal can not be reserved teeth using ul-trasonic scaler row scaling postoperative with 3% hydrogen peroxide,0.9% Sodium chloride injection alternately flushing periodon-tal pocket,removing residue until the inflammation control after the break-adjusted+appropriate amount of Minocycline hydrochlo-ride ointment,along with fine needles inserted into the periodontal pocket wall slowly until bag after pushed into the bottom of Mi-nocycline hydrochloride ointment,slow retreated the drug overflow until the gingival margin,no gargle and drinking water within half an hour,once a week. Observation group additionally received 1.2 g Huanglian shangqing soft capsules,twice a day. 4-week was regarded as 1 course,and it last 2 courses. Clinical efficacy,PLI,PD,SBI,IL-1β,IL-6,IL-2 and IL-17 levels before and af-ter treatment,and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,the difference was statistically significant (P<0.05). After treatment,the PLI, PD,SBI,IL-1β,IL-6,IL-2 and IL-17 levels in 2 groups were significantly lower than before,and observation group was lower than control group,the differences were statistically significant(P<0.05). The incidence of adverse reactions in observation group was significantly higher than control group,the difference was statistically significant (P<0.05),but the symptoms were mild. CONCLUSIONS:Based on conventional treatment,Huanglian shangqing soft capsule shows significant efficacy in the adjuvant treatment of inflammation around the implant,it can reduce inflammatory cytokine levels in gingival sulcus fluid,but with more ad-verse reactions.

Objective To study the antibacterial constituents in the fermentation broth of marine actinomyces N331.Methods The antibacterial components were isolated and purified from the fermentation broth of strain N331 by absorption with macroporous resin and silica gel column chromatography,their physiochemical characteristics and bioactivities were preliminarily investigated.Results A crystal ingredient with bioactivities was isolated from the fermentation broth and it was identified as alkaloids which was composed of several compounds with approximate polarity,and its the minimum inhibitory concentration against sensitive and drug-resistant Staphylococcus aureus was 4?g?mL-1,and its half maximal inhibitory concentration(IC50) was 2.0,3.0?g?mL-1 respectively against KB and lung cancer A549 cells,which was equivalent to cisplatin.Conclusion Marine actinomyces N331 can produce the alkaloidal substances with strong antibacterial activity and cytotoxicity.

Objective To observe and analyze the effect of dexamethasone on the adult osteoblast proliferation and differentiation in vitro. Methods Iliac trabecular bone specimens were obtained from eight adult patients undergoing scheduled surgery. After the bone pieces were treated with collagenase trypsin, larage numbers of osteoblasts were released out. Then the osteoblasts were purified and their passages were fed with DMED-F12 and fetal bovine serum. The cells were incubated under the condition of 37 ℃, 95% relative humidity and 5% CO2. Subsequently, 1?10-8 mol/L dexamethasone were added into culture medium to feed eight groups of osteoblasts for three days and the control groups were cultured not to add medicine at the same time. All the groups were observed continually under light microscope and transmission electron microscope. Finally, apoptosis detection was performed by use of the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and biochemical indexes alkaline phosphatase (ALP) and osteocalcin (OCN), were assayed to check if dexamethasone has any effect on proliferation and differentiation of osteoblasts in vitro. Results Of the adult osteoblasts obtained by collagenase trypsin method, the survival condition was good, the biochemical indexes were stable, the degree of purification was excellent, and they were fit for the next step study. The following data demonstrated that under the condition of dexamethasone 1?10-8 mol/L and osteoblasts 10 000 /ml, there were significant increase of ALP and OCN secretion without apoptosis alteration. Conclusion Dexamethasone has significant promoting effect on proliferation and differentiation of adult osteoblasts in vitro without apoptosis alteration and could be used as positive control during screening research of osteoporosis.

Objective To investigate indications,technique,prognosis and complications of anterior flap hemipelvectomy (hemipelvectomy using a quadriceps femoris muscle flap with superficial femoral artery)in the treatment of pelvic tumors.Methods From April 2009 to October 2010,10 patients with pelvic tumors underwent anterior flap hemipelvectomy in our department.There were 9 males and 1 female,aged from 30 to 62 years (average,46 years).There were 7 cases of chondrosarcoma,1 case of epithelioid sarcoma,1 case of pleomorphic liposarcoma and 1 case of spindle cell sarcoma.Results Nine patients were followed up for 12 to 30 months (average,21.5 months).Superficial skin necrosis occurred in 1 patient,while there was no wound infection.Local recurrence (33.3％) occurred in 3 patients,including 2 cases of chondrosarcoma and 1 case of epithelioid sarcoma.Three patients died at last,including 2 patients with local recurrence and 1 with pulmonary metastasis from liposarcoma.Two patients survived with pulmonary metastasis,and remaining 3 patients survived without recurrence and metastasis.Conclusion Anterior flap hemipelvectomy is indicated for:(1) patients with extensive soft tissue sarcoma in the buttock,and the tumor or reaction zone involve the subcutaneous tissue; (2) patients with recurrent pelvic tumors,and the conventional posterior flap can't be used due to contamination by the original incision; (3) patients whose posterior flap has poor blood supply due to radiotherapy or other factors.The prerequisite of the surgery is that the blood stream between external iliac artery and femoral artery is unobstructed; moreover the blood vessels are not in the tumor reaction zone.Compared with conventional posterior flap,the anterior flap hemipelvectomy is an easier procedure,which not only can easily cover surgical defect and obtain better surgical margin,but also has less complications.

The status quo of American informatics nurses investigated 4 times by US HIMSS from 2004 to 2014 was analyzed.The following characteristics of American informatics nurses were summarized in terms of their educa-tion level, income, qualification authentication, occupation satisfaction, work experience and duties, including high education level and interdisciplinary background, high income and occupation satisfaction, necessary clinical background, and systematic professional development, which may provide reference for nursing staff in China to have a better understanding of informatics nurse.

Isoprene is an important precursor of synthetic rubber material. In our previous study, metabolic engineered Escherichia coli strain (BW-01) was constructed and used to produce isoprene. Based on the theory of protein budget, using synthetic biology strategies including the increased copy number of genes and rare codons, we regulated the expression of key enzyme to improve isoprene production in Escherichia coli strain. Under shake-flask conditions, isoprene productivity of the engineered strain (BW-07) increased by 73% compared with BW-01, reached 761.1 mg/L. It provides a reference for further studies.
Butadienes ; Escherichia coli ; genetics ; metabolism ; Gene Dosage ; Hemiterpenes ; biosynthesis ; Industrial Microbiology ; Metabolic Engineering ; Mevalonic Acid ; Pentanes ; Synthetic Biology

Adult ; Arteries ; anatomy & histology ; Asian Continental Ancestry Group ; Female ; Humans ; Larynx ; anatomy & histology ; blood supply ; Male ; Microsurgery

We made the third party medical disputes mediation organization dispose medical disputes more quickly and effectively by analyzing its theoretical knowledge,running mode,economic framework,administrative agency and personnel disposition,which provided institutional insurance for the establishment of harmonious relationship between doctors and patients in our country.

Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.