Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Transplantation is regarded as the only therapeutic choice for end-stage organ failure,however,rejection restricted its efficacy after transplantation.MSCs exhibit several desirable characteristics,which may advocate their use in organ transplantation.This includes the capacity to suppress alloreactive and autoimmune T-cell responses,and pro mote a cytokine environment which is likely to be graft protective.This review describes the recent research of MSCs used in organ transplant.

Objective To explore the relation between single umbilical artery with other malformations and pregnancy outcome,and to provide evidence for pregnancy counseling and diagnosis.Methods The outcomes of 92 fetuses with single umbilical artery diagnosed and delivered in Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University from September 2007 to July 2009 were followed up and retrospectively analyzed with Chi-square test.Results Among 25 292 pregnant women,92 cases were finally diagnosed as single umbilical artery giving the incidence of 0.36％,among which 57.6％ (53/92) of newborns survived without malformations,15.2％(14/92) survived with malformations and 27.2％ (25/92) died during perinatal period.There were 36 fetuses (39.1％,36/92) with single umbilical artery complicated with other malformations,among which 29 had single malformation (80.6 ％ ),and seven ( 19.4 ％ ) had multiple malformations.The incidence of heart defects was the highest among the complicated malformations,and then followed by central nervous system,digestive system,motor system and urinary system.Thirty-three fetuses with single umbilical artery accepted karyotype analysis.Chromosome abnormality was diagnosed in three subjects (9.1％,3/33).Twenty-three fetus (25.0％,23/92) associated with small for gestational age,and among which 18 fetuses (78.3％,18/23) died with a higher mortality than that (4.0％,24/597) of small for gestational age patients without single umbilical artery (x2=181.71,P＜0.01).Conclusions Single umbilical artery is likely to be complicated with congenital anomalies.After single umbilical artery was diagnosed by routine ultrasound,fetal ultrasonography,echocardiography and amniocentesis for karyotype analysis is suggested.Fetal growth restriction is an important indicator of fetal adverse outcomes.

Transjugular intrahepatic portosystemic shunt (TIPS) has been developed for two decades in order to treat portal hypertension accompanying with upper gastrointestinal hemorrhage and refractory ascites.TIPS may cause the development of hepatic encephalopathy (HE).TIPS induces portalsystemic shunt and leads to hemodynamics chaos. Hepatic encephalopathy belongs to the complications of the portal hypertension. There are many possible predictors of the post-TIPS HE. The relationship between TIPS and HE was reviewed.

Objective To explore psychological defense mechanism and its relationship with personality in patients with hysteria(HyS). Method Both HyS patients (n=30) and normal control(NC, n=66) comple-ted Defense Style Questionnaire (DSQ) and Eysenck Personality Questionnaire(EPQ) . Results The scores of projection, isolation, somatization and the factor scores of immature defenses were significantly higher in HyS pa-tients(3.64±1.45, 5.63±2.29,5.33±2.36 and 4.74±1.27, respectively) than those in NC [(2.61±1.09,4.33±2.02, 3.86±1.60 and 3.98±0.91, respectively) (all P<0.01)]. The scores of undoing, idealization, pseudo-altruism and the factor scores of neurotic defenses were also significantly higher in HyS patients compared with NC(P<0.01). The scores of suppression, humor and the factor scores of mature defenses and the lie were found lower significantly in HyS patients compared with NC (P<0.05). The scores of the neuroticism, psychoticism and lie subscale of EPQ in HyS patients were significantly higher than that of NC(P<0.01). The scores of extro-vert subscale were not found difference significantly between HyS patients and NC. The results of correlation analy-sis showed that the scores of the psychoticism subscale of EPQ in HyS patients correlated negatively with the factor scores of mature mechanism and the scores of suppression. Conclusion The HyS patients were more likely than the normal control to endorse the immature mechanism and neurotic defense and less likely to endorse the mature mechanism, which related with unhealthy personality, especially the psychoticism.

Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.

This review snmmarized and estimated the management of cataract blindness in China recently,and introduced the purport of clinic medical economics.Based on it,it was proposed that our service system of cataract blindness could use the experiences of other countries as reference and engaged in more investigations in search of Chinese way for cataract blindness.

Catheter-Related Infections ; drug therapy ; prevention & control ; Catheters, Indwelling ; adverse effects ; Humans ; Intensive Care Units, Neonatal ; Thrombosis ; prevention & control

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use