Objective To study the distribution and drug‐resistance of pathogens isolated from vaginal secretion in the patients with vaginitis in Dalian area so as to provide an evidence for clinical diagnosis and treatment .Methods The statistical analysis was performed on the detection results of pathogens isolated from vaginal secretion in 5 308 patients with vaginitis .Results In 5 308 patients with vaginitis ,the routine detection of vaginal secretion found that 810 cases(15 .3% ) were bacterial vaginosis ,1 110 cases (20 .9% ) were candida positive and 60 cases (1 .1% ) were trichomoniasis positive ,in which 3 526 cases were performed the patho‐gen detection ,2 615 strains of pathogen were isolated ,including 1 948 strains (74 .5% ) of mycoplasma ,136 strains (5 .2% ) of Chlamydia ,17 strains (0 .7% ) of Neisseria gonorrhoeae ,276 strains(10 .6% ) of candida ,140 strains (5 .4% ) of Gram‐positive cocci and 98 strains(3 .7% ) of Gram‐negative bacilli .The susceptibility rates of Ureaplasma urealyticum to ofloxacin ,levofloxacin ,spar‐floxacin and roxithromycin were lower ,which were 7 .8% ,18 .2% ,26 .3% and 27 .2% respectively ,the susceptibility rates to clar‐ithromycin ,doxycycline and minocycline were higher ,which were 89 .4% ,99 .8% and 99 .9% respectively .The drug resistance of Mycoplasma humenis was higher .The more sensitive antibacterial drugs were only josamycin ,doxycycline and minocycline ,the sen‐sitivity rates were 75 .3% ,96 .2% and 97 .3% respectively ,which to other antibacterial drugs were less than 7 .0% .Candida albi‐cans was generally sensitive to antifungal drugs .The susceptibility rates of Staphylococcus aureus to commonly used antibacterial drugs were higher .All of 24 strains of Streptococcus agalactiae were sensitive to penicillin .No Gram‐positive coccus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to imipenem and meropenem was found .Conclusion The pathogens causing vaginitis are very complex .In the treatment of female vaginitis ,clinicians should rationally use antibacterial drugs according to the results of pathogens detection and drug susceptibility test .

microRNA (miRNA) is a class of endogenous small non-coding RNA (20-25 nt) which can regulate the expression of protein-coding genes post-transcriptionally and participate in a series of critical biological processes including cell proliferation,differentiation,metabolism,apoptosis,et al.Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma subtype.DLBCL is defined as the malignant lymphoma which originates from the B cells,with aggressive clinical behaviors and significant clinical heterogeneity.In recent years,the researches on miRNA in DLBCL have shown that several kinds of miRNA directly involved in the occurrence and development of tumors and there is a correlation between the miRNA expression profiles and molecular subtypes of DLBCL.As a kind of new molecular target,miRNA has a broad prospect in the application both for diagnosis and biotherapy of DLBCL.

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase(iNOS) in ovarian neoplasm,and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method.Results(1)The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(9.09%,13.13%,65.31%;18.18%,20.00%,61.22%) P

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase in ovarian neoplasm and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method. Results ①The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(909%、13.13%、65.31%;18.18%、20.00%、61.22%) (P

Objective:To investigate the clinical features,pathological changes of 34 cases that had suffered infantile hepatic glycogen storage disease in order to improve the recognition.Methods:The clinical manifestations of 34 cases of infantile hepatic glycogen storage disease during May 2005 to May 2009 were collected.The hepatic tissues from liver biopsy were observed under microscope and electron microscope.Results:Hepatomegaly and growth retardation were the main clinical manifestations of hepatic glycogen storage disease, partial cases were complicated with the manifestations of hypoglycemia.Hypoglycemia,hyperlactacidemia and abnormality of liver function were detected.And the pathology features were observed under microscope and electron microscope.Conclusion:The clinical manifestations of infantile hepatic glycogen storage disease were common,and the final diagnosis can depende on liver biopsy.

Objective] The article summarizes the experience of using sanren decoction to treat pediatric diseases by Professor Liu Lin, the famous TCM physician of Hubei. [Methods] Through following Professor Liu Lin clinical diagnosis, organize his medical records, summarize the experience and thoughts of using Sanren decoction to treat pediatric diseases, and for some cases. [Results] Professor Liu Lin believes that the physiological and pathological characteristics of children prone to cause hot and humid accumulation, pediatric Chih-Yin is not substantial, with their lungs often delicate and spleens often inadequate, they prone to get hot and humid accumulation after sick, and later appear a variety of symptoms including upper burner, center burner and lower burner. Therefore, the treatment of pediatric disease should take the heat into account, bearing in mind the residence site of heat evil and syndrome differentiation. Professor Liu Lin makes good use of Sanren decoction to treat a variety of pediatric diseases, judging the residence site of heat evil and clinical manifestations, giving the corresponding treatment flexibly. Such as the treatment of bronchitis with almonds as the monarch herb to expectorant cough, treatment of infantile anorexia with White Kou Ren as the monarch herb to activate spleen and remove dampness, treatment of children with nephrotic syndrome with Yiyiren as the monarch herb for penetration of water, treatment of HFMD with the Sanren to regulate Qi of triple burner, according to the symptoms to medicate and always has good clinical effect. [Conclusion] Professor Liu Lin using Sanren decoction to treat pediatric diseases is effective, his thought is worthy of depth study and promotion.

Objective:To study the inhibitory effects of adenovirus mediated vascular endothelial cell growth inhibitor 151(VEGI_(151)) on venous endothelial cell proliferation. Methods: VEGI_(151) gene was cloned into adenovirus vector pCA13 and the product was packaged and amplified with 293A cells.The expressed protein was identified by Western blot. X-gal staining was used to determine the transfection efficiency of the recombinant adenovirus system. The inhibitory effect of Ad-VEGI_(151) on ECV304 cell proliferarion was observed and assessed by violet crystal assay. The protein expression of VEGI_(151) gene in ECV304 cells was detected by immunohistochemistry. Results: Liposome-mediated pCA13-VEGI_(151) and pJM17 cotransfection of 293A cells (through homologous recombination of intracellular plasmid) is a practical and feasible method to obtain high-titre recombinant adenovirus. The constructed Ad-VEGI_(151), with high transfection efficiency, significantly inhibited ECV304 cells proliferation and expressed functional protein in target cells. Conclusion: Ad-VEGI_(151) can express bioactive protein in target cells and can inhibit the proliferation of venous endothelial cells, and this may pave a new way for gene therapy of tumors and neovascular diseases.

BACKGROUND:Studies have reported that puerarin can reduce bone resorption and promote bone formation. OBJECTIVE: To further explore the effects of puerarin at different doses on the proliferation of osteoblasts culturedin vitro. METHODS: Osteoblasts isolated from the cranium of newborn rats were culturedin vitro, and then passage 3 cels were cultured in DMEM medium with the presence of 0, 20, 40, 80 and 100 μmol/L puerarin+10% fetal bovine serum for 48 hours. The viability of osteoblasts, alkaline phosphatase activity and mineral node formation were determined using MTT, alkaline phosphatase kit and alizarin red staining, respectively. RESULTS AND CONCLUSION:The viability of osteoblasts treated with puerarin at either 40 or 80 μmol/L was significantly higher than that of the control group. Alkaline phosphatase activity and the number of mineral modules were significantly increased in osteoblasts cultured with puerarin at 40 or 80 μmol/L when compared with the untreated cels. These findings demonstrate that puerarin is able to promote osteoblast proliferation in vitro.

Objective To investigate the difference of serum levels of adiponectin (APN)and its gene polymorphism +276 G/T 和-11377 C/G in Yao patients with CHD and Han CHD patients and Yao normal people in Guanxi district.Methods Yao CHD patients,Han CHD patients,Yao normal people and Han normal people,each of 100 cases were included in the study.The levels of serum adiponectin of all research object were detected by ELISA and APN+276 G/T and APN-11377 C/G genotyping were conducted by using PCR-RFLP.Results The levels of adiponectin in two groups of CHD patients were significantly lower than same race normal groups,the difference were statically significant (t=10.311,8.642,all P =0.000).The adiponectin levels of two CHD groups was not statically significant difference (t = 1.792,P =0.076);②The APN+276 G/T and APN-11377 C/G of four groups conform the Hardy-Weinberg population genetic equilibrium law (all P>0.05);③The main type of all groups APN+276 G/T was the wild type.The genotype frequencies of APN+276 G/T were no significant differences in between 4 groups (all P >0.05);④The CG and GG genotype frequency of APN-11377 C/G in two CHD groups were higher than same race normal group,the difference of Yao CHD group and Yao normal group was statically significant (χ2 =8.908,P =0.012;χ2 = 17.275,P =0.000),and the difference of Yao CHD group and Han CHD group were not statically significant (χ2 = 0.363,P = 0.834).Conclusion As with Han CHD patients,serum low APN level and APN-11377 C/G loci may be risk factors of Yao CHD patients.

Objective To explore the risk factors and control strategies of nosocomial infection in intensive care patients in the neurological department of internal medicine.Methods 422 intensive care patients in the neuro-logical department of internal medicine were retrospectively analyzed.Results The incidence rate of neurology ICU infection was 24.41%,which was significantly higher than 4.54%of the same period of nosocomial infection rate,the difference was statistically significant(χ2 =10.36,P<0.05).In the intensive care unit of the nervous system,the respiratory infection was mainly caused by respiratory infection,the incidence rate was 57.28%,urinary system was 25.24%,and digestive system was 9.71%.Neural medical intensive care unit patients aged 60 years old,time was more than or equal to 14 days in hospital,impaired consciousness,invasive procedures,use of antibiotics and a history of diabetes mellitus in hospital infection incidence increased significantly,and the differences were statistically signifi-cant(χ2 =7.65,10.76,16.87,9.43,9.25,9.87,all P<0.05).Conclusion Older patients,hospitalized time lon-ger,impaired consciousness,invasive procedures,antibiotic treatment and a history of diabetes mellitus were neural medical intensive care unit patients occurred nosocomial infection risk factors,clinical should strengthen nursing,to improve the work of prevention and monitoring to minimize nosocomial infection.