Humans ; Respiratory Syncytial Virus Infections ; prevention & control ; therapy ; Respiratory Syncytial Viruses

Acetates ; adverse effects ; pharmacology ; therapeutic use ; Animals ; Asthma ; drug therapy ; Humans ; Leukotriene Antagonists ; pharmacology ; Quinolines ; adverse effects ; pharmacology ; therapeutic use ; Receptors, Leukotriene ; drug effects

OBJECTIVETo explore the molecular biological mechanism of ZHU's Tiaojing Cuyun Recipe (TCR) for treating anovulatory infertility patients with Shen deficiency syndrome (SDS) by observing its clinical efficacy.

METHODSUsing randomized blocking methods, 80 patients were assigned to the treatment group (40 cases) and the control group (40 cases). Patients with regular menstrual cycle started medication from the 5th day of menstruation. Those with irregular menstrual cycle first took progesterone till withdrawal bleeding ,and then started medication from the 5th day of vaginal bleeding. Patients in the treatment group took ZHU's TCR, one dose per day, while those in the control group took Clomifene Citrate (CC), 50 mg per day. Three menstrual cycles consisted of one therapeutic course, a total of 2 courses. Clinical efficacy such as pregnancy rates and abortion rates were observed. Ovulation indices (the maximal diameter of mature follicles, luteinized follicles, ovulational follicles, and the endometrial thickness on the ovulation day), SDS, and integrals of menstrual symptoms were monitored before and after treatment. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH) , and estradiol (E2) were determined using chemiluminescent immunoassay before treatment and after on therapeutic course. Serum levels of activin A (ACTA), inhibin B (INHB), and follistatin (FS) were detected using double antibody sandwich ELISA.

RESULTSCompared with the control group, the pregnancy rate was obviously elevated and the abortion rate was obviously lowered in the treatment group (P <0. 05). Ovulation rates of mature follicles and luteinizing follicles decreased more in the treatment group (P < 0.05). Compared with before treatment, integrals for SDS were lower, the maximal diameter of pre-ovulational follicles was increased, and integrals for menstrual symptoms in non-pregnant patients of the two groups were obviously lowered. Meanwhile, the endometrial thickness on the ovulation day was increased in the treatment group after treatment, but reduced in the control group (P < 0.05, P < 0.01). Compared with the control group, integrals for SDS were decreased, and the maximal diameter of pre-ovulational follicles was lowered in the treatment group after treatment (P < 0.05, P < 0.01). Integrals for SDS and the difference in the endometrial thickness on the ovulation day were increased, but the difference in the maximal diameter of pre-ovulational follicles were reduced (P < 0.05, P < 0.01). In the treatment group serum levels of E2 and ACTA increased more after one therapeutic course than before treatment (P < 0.01), but serum levels of INHB and FS decreased more after one therapeutic course than before treatment (P < 0.05). In the control group serum levels of FSH and ACTA increased more, and the serum level of FS decreased more after one therapeutic course than before treatment (P < 0.05, P < 0.01). Compared with the control group, serum levels of FSH and ACTA increased more, and serum levels of INHB decreased more in the treatment group after one therapeutic course than before treatment (P < 0.05, P < 0.01).

CONCLUSIONSZHU'sTCR could improve SDS of anovulatory infertility patients, regulate the follicular development, and elevate the pregnancy rate. Its actions might be associated with regulating their sex hormones, expressions of ovary local factors such as INHB, ACTA, and FS.

Activins ; Clomiphene ; Drugs, Chinese Herbal ; therapeutic use ; Estradiol ; Female ; Follicle Stimulating Hormone ; Follistatin ; Humans ; Infertility, Female ; complications ; therapy ; Inhibins ; Luteinizing Hormone ; Medicine, Chinese Traditional ; Ovarian Diseases ; Ovarian Follicle ; Ovulation ; Progesterone

OBJECTIVE:To evaluate therapuetic efficacy and toxic reaction of 2 therapuetic regimens for 56 cases of bone me-tastasis of cervical cancer. METHODS:56 cases of bone metastasis of cervical cancer with complete information were collected ret-rospectively and randomly divided into radiochemotherapy group(31 cases)and diphosphonate group(25 cases). Radiochemothera-py group was given radiotherapy,chemotherapy and diphosphonate;diphosphonate group was given palliative symptomatic treat-ment of diphosphonate;a treatment course lasted for 3-4 weeks,and both groups received 3 courses of treatment. Average survival time,survival quality and toxic reaction were comparison in 2 groups. RESULTS:After bone metastasis of cervical cancer,the av-erage survival time of radiochemotherapy group was 17 months (95%CI:9.1-24.9),and that of diphosphonate group was 19 months (95%CI:2.4-35.6);3 years later,the survival rate of radiochemotherapy group was 16.1%,and that of diphosphonate group was 16.0%,there was no statistical significance(P=0.820). To compared KPS after 3 treatment courses,the improvement rate of radiochemotherapy group(22.6%)was significantly lower than that of diphosphonate group(40.0%),with statistical signif-icance (χ2=4.36,P<0.05). Toxic reaction of radiochemotherapy group was more significant than that of diphosphonate group. CONCLUSIONS:Diphosphonate has little effect on survial quality and slight toxic reaction. It is worthy of spreading in some pa-tients with advanced,elderly and reccurred bone metastasis of cervical cancer.

Human fetal esophageal epithelial tissue were cultured in vitro and treatedwith mycotoxins of Alternaria alternata (AME or AOH) for 4 h. The genomic DNAwere extracted from these tissues. Genomic DNA was isolated from normal human fetalesophageal epithelium (as blank control), DNA from malignant tissue and its adjacentnormal mucosa was obtained from esophagectomy patients. DNA was amplified with PCRreaction, using genomic DNA as templet. The PCR products was a 104bp fragment from which the 12 codon of c-Ha-ras gene was contained. The excition point of restriction en-zyme Hpe Ⅱ was located in this fragment. The PCR amplified 104bp fragment was diges-ted by Hpa Ⅱ and analysed by agarose gel electrophoresis. The results showed that the104bp fragment amplified from genomic DNA of blank control and esophagectomy patientcould be digested by Hpa Ⅱ ; but that from genomic DNA of human fetal esophagealepithelium treated by AME or AOH could not. These results indicated that a mutationhad taken place at 12-codon of c-Ha-ras gene after it was treated by AME, AOH for ashort time. The mutation of Ha-ras gene might be the early event during esophageal car-cinogenesis. The effect of AME and AOH during the onset of esophageal cancer and themolecular machanisms of the effect were worth of further study.

Objective To investigate whether high cholesterol diet (HCD) can cause gallbladder cholesterol calculus and to probe into the mechanism of its influence on gallbladder motility function. Methods Noumenon dissect and B-type ultrasonic apparatus was used to observe condition of gallbladder calculus formation in HCD group and normal control group; gallbladder motility function of the two groups were measured; radioimmunoassay was used to measure plasma CCK level in fast and 30min after fatty meal; colorimetry was used to measure cholesterol concentration in bile; and the pathologic changes of gallbladder specimen were observed. Results The gallbladder cholesterol calculus formation rate and cholesterol concentration in bile of HCD group was remarkably higher than that in normal control group (P

A study was made; of the effect of malotilate on the acute liver injury induced by carbon tetrachlorid,e ( CC14 ) and d-galactosamine in mice. Malotilate ( 50~150mg/kg ig?3 ) significantly inhibited the elevation of serum glu tamic pyruvic transaminase ( SGPT ) in CC14- intoxicated mice.At the dose of 100mg/kg ig?3, malotilate remarkably increased the content of hepatic glycogea in CCl4-injected mice. The contents of serum protein, liver protein, and cytochrom P-450 in liver hemogenate were increased by malotilate ( 100mg/kg ig?3) in CC14-intoxicated mice. The drug also reduced the accumulation of liver triglycerides induced by CCl4 in mice.In addition to, malotilate(50mg /kg, ip?5) could act against the increase of SGPT and the decrease of liver protein content induced with d-galactosamine in mice. These results suggest that malotilate may be a new therapeutic agent for liver injury.

Objective To investigate the effect of pidotimod combined with antiviral drugs on immunologic function and myocardial enzyme spectrum in children with infectious mononucleosis.Methods 94 cases of children with infectious mononucleosis selected in Wenzhou Children's hospital were randomly divided into control group and research group,47 cases in each group.The control group were given conventional antiviral treatment; On this basis,the research group were given pidotimod,2 weeks for one period of treatment.Before and after treatment,immune function,cell factors and myocardial enzyme spectrum were tested,the clinical symptoms,signs,the curative effect and complications were observed and compared.Results Compared with before treatment,after treatment,serum CD4+,CD4+/CD8+ IgA and IgG increased,CD8+ decreased,TNF alpha,IL-6,AST,LDH,CK,CK-MB decreased(P<0.05).Compared with the control group,in the research group,CD4+,CD4+/CD8+,IgA and IgG content is higher,CD8+ content is lower,the TNF alpha,IL-6,AST,LDH,CK,CK-MB content is lower(P<0.05).The time of antipyretic,angina fade,narrow lymph nodes,liver and spleen shrinks and length of hospital stay in the research group were shorter than that in the control group(P<0.05).The effective rate was 74.47％in the control group,lower than 91.49％of research group(P<0.05).Conclusion The curative is exact that pidotimod combined with antiviral drugs on the treatment of infectious mononucleosis.It could improve immune function,reduce myocardial enzyme spectrum and inflammation.

Frailty is an age-related non-specificity status caused by a decline of physiological reservation,which leads to an increase of vulnerability in body and decrease of ability in stress resistance.The pathophysiology of frailty involves multiple systems including neuromuscular system,metabolism and immune system.Studies have showed that frailty is closely related with negative events of elder people.People with frailty are subject to higher risks for fall,hospitalization,and mortality.However,the development of frailty can be delayed if early prevention or interventions are performed.Prospective cohort studies have showed that the risk factors for frailty include age,gender,family history of obesity,BMI,waist circumference,blood pressure,hyperglycemia,smoking and lack of exercise.Among them,nutrition,exercise and therapeutic drugs are hot spots in prevention study.Here,we reviewed the intervention studies by focusing on nutrition,exercise and therapeutic drugs in frail population.