Objective To investigate whether high cholesterol diet (HCD) can cause gallbladder cholesterol calculus and to probe into the mechanism of its influence on gallbladder motility function. Methods Noumenon dissect and B-type ultrasonic apparatus was used to observe condition of gallbladder calculus formation in HCD group and normal control group; gallbladder motility function of the two groups were measured; radioimmunoassay was used to measure plasma CCK level in fast and 30min after fatty meal; colorimetry was used to measure cholesterol concentration in bile; and the pathologic changes of gallbladder specimen were observed. Results The gallbladder cholesterol calculus formation rate and cholesterol concentration in bile of HCD group was remarkably higher than that in normal control group (P

BackgroundAdenoid cystic carcinoma is a malignant tumor originating from lacrimal gland epithelium with high recurrence rate and mortality because of its invasiveness. Although surgery, radiotherapy and chemotherapy are used clinically,the curative effect is not enough satisfied. ObjectiveThis study was to provide an experimental basis for clinical application of 125 Ⅰ seeds interstitial brachytherapy. MethodsHuman ACC-2 cell links were transplanted subcutaneously in the back of 30 SPF BALB/C nude mice to establish the ACC model,and 25 of these mice with suitable sizes of tumor were selected and randomly divided into the G1 (0. 4 mCi 125 Ⅰ ), G2 (0. 6 mCi 125 Ⅰ ) ,G3(0. 8mCi 125 Ⅰ ) ,and G4( 1.0mCi 125 Ⅰ ) groups according to the different therapeutic radioactivity treatments,with 5 nude mice for each group,and a 125 Ⅰ seed without radioactivity was used in 5 mice as the control group. The dimensions of the tumors were measured at 2-day intervals and the inhibition rates of tumors were calculated. Nude mice were killed 14 days later by the broken neck method,and the amount of apoptosis and necrosis as well as the maximum effective radius of tumor to 125Ⅰseed were detected under the transmission electron microscope and routine pathological examination. ResultsFourteen day after operation, the dimension of tumors was (3713.19±243.23)mm3 in the G0 group;while in the G1 ,G2 ,G3 and G4 groups,the dimensions of tumors were (3113.35±316.54) mm3,(2635.85±261.21) mm3, (2538.37±312.16) mm3 and(1686.28±231.65) mm3,respectively, showing a significant decrease in comparison with the Go group( P＜0. 05 ). The tumor inhibitory rates in the G1 ,G2,G3 and G4 group were(20. 11±3.09)％, (36. 18±2.54)％ ,(40. 83±4. 17)％ ,and(66. 63±5.34)％ ,with an obvious elevation with the increase in the dose of 125 Ⅰ ( F=120. 240,P=0. 000). Correlative analysis showed that the intensity of radioactivity from 125Ⅰhad a positive correlation with tumor inhibitory rate (r =0. 653,P =0. 008 ). The maximum effective radius were ( 5.2 ±0.5 ) mm, ( 6.4 ±0. 7 ) mm, ( 7.4 ±0.4 ) mm, and ( 8.2 ±0. 5 ) mm in the G1 ,G2, G3 and G4 groups, with the considerable differences among them (F=29. 22, P=0. 000). Radioactivity of 125 Ⅰ exhibited positive correlation with the maximum effective radius ( r =0. 609, P =0. 004). Conclusions125 Ⅰseed implantation brachytherapy can inhibit the growth of the transplanted ACC in BALB/C nude mice by suppressing the proliferation of tumor cells. It is a safe ,feasible and effective method to treat adenoid cystic carcinoma.

Objective: To explore the predictors of drive for thinness in female college students in Beijing. Methods: 499 female students were investigated with Drive For Thinness of EDI, Body-Esteem Scale for Adolescents and Adults and BMI-based Silhouette Matching Test (BMI-SMT). Results: The deviation of perception, the deviation of expectation of one's own body and body-esteem were found to be predictors of drive for thinness. For someone with normal weight, the deviation of perception, the deviation of expectation of one's own body were significantly larger than someone slightly underweight and very underweight, the body-esteem were significantly lower and their drive for thinness were significantly greater. Conclusion: The distorting perception, the unreasonable expectation of one's own body and low body-esteem are important predictors of drive for thinness, which will then cause eating disorder in female college students.

Objective A biomechanical comparison of three kinds of internal fixation for intercondylar fracture of humerus was, made so as to provide a theoretical basis for clinical practice. Methods 18 fresh humeri were used to reproduce T-type intercondylar fracture, and three different fixation appliances were applied, including cross Kirschner wire with addition of “8” wire, single “Y” type plate, 1/3 tubular plate with reconstruction plate. Biomechanical tests were performed on these specimens. Result Under the same load condition, 1/3 tubular plate with reconstruction plate yielded the best results, while crossing Kirschner wire with “8” wire was the poorest. Conclusion The results showed that 1/3 tubular plate with reconstruction plate was a good internal fixation appliance. It has a proper design and stability, but it is not easy to apply. Therefore, an optimal method should be chosen according to local condition in clinical practice.

Objective To study the therapeutic effect of anisodamine on pulmonary edema after sea-water drowning(PE-SWD). Methods Rat models of PE-SWD were set up by infusing sea-water into rats lungs,and then were randomly divided into PE-SWD model group(M group) and therapeutic group with anisodamine(T group). Blood-gas indexes of arterial blood were measured at 0.5, 1, 2, 4 and 6 hours after infusion. TNF-? levels in the plasma,bronchoalveolar lavage fluid (BALF) and lung tissues were detected by ELISA. At the same time,the dry/wet weight of lung was also measured. Results The levels of blood PaO_2,SO_2 and pH in T group were obviously higher than those in M group 2h after infusion. The dry/wet weight of lung in T group was lower than that in M group. The concentration of TNF-? in plasma, BALF and lung tissues in the both M and T groups significantly higher than that in control group, and reached maximum 1h after infusion. After infusion,the TNF-? contentration in all tested specimens in T group was obviously lower than that in M group. Conclusion There was the therapeutic effect of anisodamine on the pulmonary edema after sea-water drowning possibly by decreasing TNF-? level.

Objective To investigate the expression of Pin1 and cyclin D1 in human pancreatic carcinoma and to discuss its role in oncogenesis of pancreatic cancer.Methods The mRNA expression of Pin1 and cyclinD1 in pancreatic tumor tissues and corresponding adjacent nontumor tissues 27 patients was detected by the real-time quantitative reverse transcription-polymerase chain reaction(RQ-RT-PCR).The results of RQ-RT-PCR were analyzed by one-way ANOVA and Fisher's exact probabilities test.Results Cyclin D1 and Pin1 were overexpressed at mRNA level in pancreatic carcinoma tissues compared with their adjacent nontumor tissues.Cyclin D1 overexpression were found in 14 of 20 pancreatic carcinoma tissue specimens and Pin1 overexpression in 13 of 20 carcinoma tissue specimens.The expression of cyclin D1 and Pin1 in pancreatic cystadenoma tissues was not different than that of corresponding adjacent nontumor pancreatic tissue.Pin1 overexpression positively correlated with an increase in cyclin D1 levels as shown by Fisher's exact probabilities test.However,Pin1 and cyclin D1 expression was not correlated with clinical stage and pathological parameters.Conclusions The overexpression of Pin1 in pancreatic carcinoma tissues could promote cyclin D1 expression,which might be a critical event in oncogenesis of pancreatic carcinoma.Pin1 may play a key role in pancreatic carcinoma.

Objective To evaluate effectiveness of ex vivo adenoviral gene transfer creating human bone morphogenetic protein 7-producing mesenchymal stem cells (Ad-hBMP-7 cells) compounded with nano-hydroxyapatite/collagen(NHAC) composite to induce spinal fusion in a rat posterolateral spine fusion model. Methods Mesenchymal stem cells were cultured and transduced by Ad-hBMP-7. Then Ad-hBMP-7 cells were cocultured with NHAC. Totally 56 Wistar rats were divided into four groups. Intertransverse spinal arthrodesis was attempted in four groups of Wistar rats with autogenous bone (group A); NHAC(group B); NHAC and BMSCs (group C); Ad-hBMP-7 cells and NHAC (group D). Each specimen underwent plain radiography, manual palpation and histological analysis. Results All spines in group D had fusion. In contrast, none of the spines in group A and group B had fusion. Three of ten rats in group C achieved fusion at the 12th week post-operatively.Conclusion BMP-7-producing mesenchymal stem cells compounded with NHAC composite may induce intertransverse fusion in the rat spine model.

Objective To explore the value of serum CA125 in prediction of the first-trimester threatened abortion.Methods The serum CA125 level of 78 patients with threatened abortionand( miscarriage success group and failure group) and 40 normal early pregnant women before treatment and after 1 ～ 4 weeks were tested by chemiluminescent immunoassay(CLIA) methods and the results were compared.Results The serum CA125 level of the failure group was significantly higher than that of the miscarriage success group[ (28.52 ± 19.12) x 103 U/L] and normal early pregnant women group [ (20.45 ± 9.55) × 103 U/L] ( t =- 1.28,- 1.24,all P ＜ 0.05 ),and it increased by degree and by time,and the sensitivity and specificity of detection was 93.1％ and 87.85％ respectively.Conclusion Monitoring of serum CA125 had highly clinical value in predicting the prognosis of threatened abortion.

The potamon that were sold by Mengla market were examined positive for two species of paragonimus metacercariae andexcyted metac ercariae, this is the first report in Yunnan Province． The potamon that were sold by cities market from other suburbs may cause epdemic or outbreak of paragonimiasis, there is of momentous significance in epidemiology．

Objective To investigate the relationship between plasma homocysteine (Hcy) levels and TCM syndromes of dementia.Methods 250 cases were divided into a dementia group (120 cases) and non-dementia group (130 cases).120 cases in the dementia group include 36 Alzheimer dementia(AD) patients and 84 vascular dementia (VaD) patients.The plasma levels of Hcy were measured in all 250 cases.The relationship between Hcy levels,TCM syndromes and dementia was analyzed.Results The level of plasma Hcy was significantly higher in the dementia group (17.13 ± 9.01) nmol/ml than that in the non-dementia group (12.81 ± 7.94) nmol/ml,and it was highest in the VaD patients (P＜0.01).The mean of Hcy concentration in obstruction of phlem-turbid syndrome was higher(18.43±7.81)nmol/ml than syndromes [Tanyuzuluo (5.38 ± 8.91)nmol/ml,Shenxusuikui (16.44 ± 9.05)nmol/ml,Shenyinkuixu(16.19 ± 9.24)nmol/ml,Ganjinghuowang (15.92 ± 7.28)nmol/m1,Pishen-Yangxu(12.26 ± 5.17) nmol/ml (P ＜ 0.01)].Conclusion Increased plasma Hcy levels may be one of the risk factors leading to AD or VaD.