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Objective To investigate the levels of interleukin 1? (IL 1?) and tumor necrosis factor ? (TNF ?) in both serum and CSF in patients with Alzheimer's disease Methods Eleven patients with Alzheimer's disease,13 patients with vascular dementia (VD) and 13 healthy controls were analyzed by using commercial ELISA kits Results The average serum level of IL 1? was found higher in AD patients(60 13?17 81 pg/ml) than in VD patients(38 51?14 39 pg/ml) and in healthy controls(33 98?16 31 pg/ml)( P

Objective Recent researches show that bone sialoprotein is closely related with metastasis of breast cancer to bone. In order to investigate the effect of bone sialoprotein in the course of metastasis of breast cancer to bone, the following study was undertaken. Methods The growth curve, clone formation and adherence of breast cancer cells experiments were carried out to study the effect of hBSP over-expression on biological behaviors of breast cancer cells. Results It was found that breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID) stably transfected with hBSP cDNA were not inhibited through proliferation assay of breast cancer cells. The activity and tumorigenicity of breast cancer cells with hBSP over-expression were enhanced. Comprising with MDA-MB-231BO non-transfected the colony formation percentage of breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID) increased from 8.90%?0.53% to 15.6%?0.94% and 12.8%?0.77%. The OD_ 620 value of adherence of breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID)increased from 0.29?0.017 to 0.52?0.031 and 0.43?0.026 respectively in cells attachment assay, which suggested that over-expression of hBSP enhances breast cancer cell MDA-MB-231BO adherence and attachment property. Conclusion The study results suggested that hBSP over-expression had autocrine effect on breast cancer cells and could enhance the ability of breast cancer cells metastasizing to bone.

In this study, we examined the effects of aspirin on the growth rates, subcellar distribution of beta-catenin protein, the expression of beta-catenin/TCF signaling pathway target gene cyclinD1 mRNA, and cell cycle of Jurkat cell line (Human T-acute lymphoblastic leukemia). Our results showed that the treatment with aspirin inhibited the growth of Jurkat cell line. Jurkat cells treated with 3 mmol/L of aspirin could significantly decrease nuclear localization of beta-catenin, and at 5 mmol/L of aspirin, the nuclear localization of beta-catenin was undetectable. QRT-PCR showed that the target gene cyclinD1 mRNA expression was gradually decreased with the dosage of aspirin. Aspirin induced G0/G1 cell cycle arrest in Jurkat cells. We are led to conclude that aspirin acts through beta-catenin-independent mechanisms. The effects of aspirin include down-regulation of beta-catenin nuclear localization and G0/G1 cell cycle arrest, which might serve as a means of growth inhibition in aspirin-treated human Jurkat cell line.

Objective:To describe PACS hardware infrastructure experimental design and summary under big data environment.Methods: A real DICOM communication test of PACS hardware infrastructure experimental design.Results: Archive test figures of DICOM communication for 64 channel CT, 1.5T MRI and DR.Conclusion: Currently, an efficient, reasonable hardware infrastructure design is essential to guarantee large hospital PACS application.

Objective To explore the influence of different hemodialysis ways on cognitive function in the patients with end-stage renal failure(ESRF) in maintenance dialysis.Methods Choosing 68 ESRF patients who needed maintenance dialysis were divided into high flux dialysis group and normal flux dialysis group,34 cases in each group.The P300 lantency period(LP) and amplitude(Amp),mini-mental status examination(MMSE) scale were applied to examine the patients in the two groups before treatment,1 month and 6 months after treatment,respectively.Results The P300 LP and Amp in high flux dialysis group and low flux dialysis group were(398.6?38.5)ms,(7.3?1.21)?V vs(410.3?49.7)ms,(6.9?1.03)?V before the treatment;and(336.8?32.1)ms,(8.2?1.15)?V vs(342.6?35.8)ms,(7.9?1.01) ?V 1 month after treatment.There were no significantly differences between the two groups.6 months after treatment,the P300 LP and Amp of high flux dialysis group and low flux dialysis group were(320.6?31.9)ms,(8.5?1.02) ?V vs(390.6?42.8)ms,(6.5?1.12)?V.There were significantly differences between the two groups(all P

Objective The K-ras gene plays a major role in the development , progression, and drug selection for the target treatment of colorectal cancer .The aim of this study was to evaluate the feasibility of detecting K-ras mutation in colorectal cancer by high-resolution melting ( HRM) analysis and to investigate the relationship between K-ras mutation and the clinicopathological parame-ters of colorectal cancer. Methods We collected the tissue samples of colorectal cancer from 179 patients, detected the mutations in codons 12 and 13 of the K-ras gene, and analyzed the relationship between K-ras mutation and the clinicopathological parameters of the patients. Results In the 179 cases of colorectal cancer, K-ras mutation was found in 77 (43.02%), significantly higher in those aged ≥60 years than in those aged <60 years (55.17% vs 33.70%, P<0.05).Multivariate logistic regression analysis showed a significant influence of age on K-ras mutation (OR=1.506, 95%CI:1.028-2.011, P<0.05). Conclusion HRM analysis is a rapid, sensitive, and inexpensive diagnostic tool for the detection of K-ras mutation, and K-ras mutation is associated with the age of colorectal cancer patients .

Arrhythmogenic Right Ventricular Dysplasia ; complications ; Autopsy ; Cardiomyopathy, Hypertrophic ; complications ; Cause of Death ; Coronary Disease ; complications ; Coronary Vessel Anomalies ; complications ; Death, Sudden, Cardiac ; etiology ; pathology ; Humans

As the actual clinical reflecting of transform Chinese medicine special curative effect, Chinese medicine preparation not only satisfies the need of hospital clinic, scientific research and teaching, but also plays an important role in deepening medical and health system reform, improving people's health level and contributing to the economic growth. However, some problems about administration and approval (tending to western medicine), contraction of the scale, lack of synchronization for clinic and scientific research, and the imbalance of regional development make Chinese medicine preparation move forwards slowly in contradiction. It has not only reduced the effectiveness of the Chinese medicine preparation in hospital clinic, but also brought bad effect on modernized development of Chinese medicine preparation. Research shows that main influencing factors of status quo of Chinese medicine preparation in medical institution include imperfect laws and regulations, high cost than income, and shortage of talents in preparation research. The analysis indicated that the necessary measures to break the contradiction, improve clinical effect of Chinese medicine, and promote the modernization development of Chinese drugs preparation were as follows: government and related departments should strengthen the supporting force in policy by adjusting the examination and approval policy, speeding up dispensing use, reforming pricing system, including into medicare reimbursement, integrating advantage resources and so on; medical institution should actively carry out research and development of traditional Chinese Medicine through drawing the traditional and modern essence, reserving professional talents, and developing characteristic preparation; companies cooperate with hospitals for complementary advantages, which can rapidly transform Chinese medicine preparation into clinical practice.
Chemistry, Pharmaceutical ; economics ; methods ; trends ; China ; Drug Discovery ; economics ; trends ; Drugs, Chinese Herbal ; chemistry ; economics ; pharmacology ; Humans ; Medicine, Chinese Traditional ; economics ; trends