Objective To investigate the effects of rosiglitazone on proangiogenesis function of human umbilical vein endothelial cells ( HUVEC) in high glucose environment. Methods HUVECs were cultured in high glucose environment and stimulated by rosiglitazone. MTT, cell scratch test and Transwell assay were used to detect HUVEC proliferation and migration. The concentration of VEGF, SDF-1 was also detected in the supernatant. Results Rosiglitazone could effectively promote HUVEC proliferation and migration. The concentration of VEGF and SDF-1 in rosiglitazone stimulated supernatant was higher than that in high glucose group. The inhibition of AKT signal could block the promotion of rosiglitazone on the HUVEC proliferation, migration and secretion. Conclusion Rosiglitazone could significantly promote HUVEC secretion, proliferation and migration in high glucose environment. AKT signal played an important role in this process.

Objective To evaluate the clinical role of F-18 fluorodeoxyglucose (FDG)-positron emission tomography (PET) for the diagnosis and staging of prostate cancer.Methods 18F-FDG PET/CT and 99Tcm-MDP ECT whole body bone scan were both performed in 40 patients with prostate cancer proved by biopsy or surgery.We then evaluated the diagnostic value of 18F-FDG PET/CT for prostate cancer.Results Of 40 cases,17 cases were detected by 18 F-FDG PET/CT with a sensitivity of 42.5％.Of the 17 cases with lymphoma metastasis,8 cases were detected by CT while 15 cases were detected by 18F-FDG PET/CT.The sensitivity of 18F-FDG PET/CT is 88.2％.Though 18F-FDG PET/CT and 99Tcm-MDP bone scan have a similar sensitivity in detecting bone metastases,18 F-FDG PET/CT has better specificity and accuracy in detecting bone metastases than that of an 99Tcm-MDP bone scan.Conclusions Though the diagnostic value of 18F-FDG PET/CT in detecting primary focus of prostate is limited,it still has an important role in detecting lymph node metastases and bone metastases.18F-FDG PET/CT has its superiority in prostate cancer staging.It can also help clinicians to select the regimen of treatment.

Objective To know the focus groups' iodine nutrition level after implementation of a new standard of salt iodization,and to provide technical support for iodine deficiency disorders (IDD) prevention in Nanping City.Methods According to the requirements of Focus Groups' Iodine Nutrition and Related Health Monitoring Program in Fujian Province,Yanping,Jian'ou,Zhenghe,Jianyang and Shaowu counties (cities,districts) were selected for monitoring in 2014;Pucheng,Songxi,Wuyishan,Shunchang and Guangze counties (cities,districts) were selected for monitoring in 2015.We studied:①The water iodine:2 tap water samples were collected from centralized water supply system;from decentralized water supply system,2 residents drinking water samples were collected according to each of five directions of east,west,south,north,and center,for determination of water iodine content.②Salt iodine:4 administrative villages (neighborhood committees) were selected from each monitoring site,from which 15 households were selected,edible salt samples were collected,and salt iodine content was tested.③Urinary iodine:one primary school was selected from each monitoring site,30-40 students aged from 8-10 years old were selected from each primary school,urine samples were collected for detecting urinary iodine.④Salt intake per capita:among the 8-10 years old students whom had been collected urine samples,10 students' family edible salt samples were collected,then the salt intake per capita was measured via 3-days weighing method.⑤ Goiter:in Jianyang and Shaowu Cities in 2014,and in Guangze and Shunchang Cities in 2015,the thyroid volume was measured using ultrasound,and goiter rate was calculated of 8-10 years old students whom had been collected urine samples before.⑥Pregnant women urinary iodine:20 pregnant women (early,middle and late pregnancy were included) were selected in each monitoring site,urine samples were collected for detecting urinary iodine.Results Investigation results in 2014 and 2015 were as follows:61 and 50 samples of daily drinking water of the two years were investigated,the medians of drinking water iodine were 1.5 and 5.7 μg/L for each group of samples,respectively;and daily salt intake of 253 and 250 residents of the two years were investigated,and the medians intake were 6.8 and 6.9 g/d,respectively;1 500 and 1 500 residential edible salt samples in the two years were tested,iodized salt coverage rates were 99.7％ (1 496/1 500) and 99.9％ (1 498/1 500),respectively,qualified rates of iodized salt were 95.7％ (1 431/1 496) and 94.5％ (1 416/1 498),respectively,qualified iodized salt consumption rates were 95.4％ (1 431/1 500) and 94.4％ (1 416/ 1 500),respectively;of which 749 and 1 000 8-10 years old children's urinary iodine were also tested,and children's urinary iodine medians were 190.1 and 210.4 μg/L,respectively;300 and 275 thyroids of children aged from 8 to 10 were tested,and goiter rates (ultrasound method) were 1.3％ (4/300) and 2.9％ (8/275),respectively;500 and 500 pregnant women urinary iodine were tested in the two years,and pregnant women urinary iodine medians were 133.2 and 150.3 μg/L,respectively,the pregnant women urinary iodine median of the Nanping City of 10 counties (cities,districts) and 7 counties (cities,districts) were less than 150 μg/L.Conclusions Nanping City's natural environment iodine is deficient.After the new standard of salt iodization,we can continue to promote the process of the standard of eliminating IDD in China,besides,8-10 years old children's iodine nutrition is basically at the appropriate level,but the level of pregnant women has showed a slightly deficiency.

Abstract: Objective To analyze the changes of coagulation indicators in patients with hepatitis B complicated with Clonorchis sinensis (C. Sinensis), and provide reference value for diagnosis, drug using and prognosis monitoring. Methods　The patient samples were collected from the Third Affiliated Hospital of Sun Yat-sen University from June 2018 to February 2022 and divided into six groups. They were 40 healthy patients, 47 patients with simple chronic hepatitis B, 47 patients with post-hepatitis B liver cirrhosis, 40 patients with C. Sinensis mono-infection, 30 patients with chronic hepatitis B patients co-infected with C. Sinensis and 27 patients with post-hepatitis B liver cirrhosis patients coinfected with C. Sinensis. Four coagulation indexes, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB), were detected and compared among the groups. Results Compared with the healthy group, C. Sinensis mono-infection group had higher level of FIB and delayed PT, APTT; chronic hepatitis B group and chronic hepatitis B patients co-infected with C. Sinensis group had delayed PT, APTT, TT and significant lower FIB, these differences were statistically significant (all P<0.05). Compared with simple chronic hepatitis B group, post-hepatitis B liver cirrhosis and chronic hepatitis B patients co-infected with C. Sinensis had significant delayed PT, APTT, TT and lower FIB (all P<0.05). Compared with the post-hepatitis B liver cirrhosis and chronic hepatitis B patients co-infected with C. Sinensis, post-hepatitis B liver cirrhosis patients coinfected with C. Sinensis group had significant delayed PT, APTT, TT and lower FIB (all P<0.05). Conclusions The coinfection of C. Sinensis will further aggravate the coagulation dysfunction of HBV patients, leading to poor treatment and prognosis. HBV patients will have worse coagulation function in the process to post-hepatitis B liver cirrhosis; Therefore, it is important to pay attention to C. Sinensis co-infection when treating HBV patients, so that further guidance on clinical use and monitoring of prognosis can be provided.

Objective To systematically evaluate the health status and intervention measures in the US Armed Forces, and to provide reference for the development of health promotion strategies in our army.Methods The PubMed, Medline, Springer, Elservier, HighWire and CNKI Database were searched electronically, with assigned search strategy for American military health status and intervention measures published from Jan.1997 to Dec.2015.Results There were 25 of pieces literature about training injury, low back pain, posttraumatic stress disorder, depression and cardiovascular disease that were screened.Conclusion The main health problems facing the US Armed Forces and the focus of the intervention measures involved training injuries, low back pain, mental illness and cardiovascular disease.Protection of military health is shifting from the single disease prevention to diversified comprehensive maintenance in the new era.

Objective To analyze the pregnant outcome of women with chronic hypertension,and to investigate the high risk factors associated with harmful maternal and perinatal prognosis.Methods Of the 14127 deliveries in Peking University First Hospital from Jan 2001 to Dec 2005,121 pregnant women with chronic hypertension were identified and divided into two groups:chronic hypertension with and without preeclampsia(group PE,64 cases;group N-PE,57 cases).The maternal and perinatal outcomes of the two groups and the high risk factors for adverse maternal and perinatal prognosis were analyzed.Results(1) The incidence of pregnancy with chronic hypertension was 0.86%(121/14127).(2)The incidences of placental abruption,pulmonary edema and retinopathy in groups PE and N-PE were 16%(10/64)vs 2% (1/57),11%(7/64)vs 0 and 41%(26/64)vs 16%(9/57,P

Adult ; Choanal Atresia ; etiology ; therapy ; Constriction, Pathologic ; etiology ; Humans ; Male ; Nasopharyngeal Diseases ; etiology ; therapy ; Otorhinolaryngologic Surgical Procedures ; adverse effects ; Palate ; surgery ; Postoperative Complications ; therapy ; Uvula ; surgery

Ubiquitin-proteasome pathway is one of the primary pathway in intracellular protein degrada-tion,therefore the ubiquitin conjugating enzyme D3(UbE2D3)which involved in the ubiquitin mineralization process can affect the biological effects accordingly to affect some protein and nucleic acid content and activity. The function that participate in modification and degradation of some cancer factors is vital in tumor cells,and followed by tumor biological behavior changing. Researches show that UbE2D3 correlates with human telomer-ase reverse transcriptase( hTERT),radiation sensitivity,aggressive,etc in breast cancer.

Objective To analyze the clinical,muscle pathological features and molecular mutations in the 2 Chinese Han siblings with limb-girdle muscular dystrophy(LGMD) and conclude the phenotype/genotype correlations.Methods Clinical and muscle pathological data were collected.Genomic DNA of the two siblings and their parents were extracted using standard procedures from the peripheral leukocytes.A custom of targeted gene panel including 61 neuromuscular genes were designed by using the Agilent Sureselect Target Enrichment Kit.Targeted next generation sequencing(NGS) was performed in the proband,and CAPN3 gene mutation was verified with Sanger sequencing in the two siblings and their parents.The dbSNP138 and http://www.dmd.nl were searched to determine the disease-causing mutations.Results The proband slowly showed muscle weakness profoundly with pelvic muscles,developed difficulty in squatting and standing and climbing stairs.She had a tight Achilles tendon,high CK level (1 908-9 241 IU/L),without winging scapula and hypertrophy calf.The affected brother was only diagnosed hyper CKemia.By using the targeted NGS,the two siblings possessed the same two compound heterozygous mutations(c.717delT and c.2243G ＞ A) in CAPN3 gene.The two mutations both were verified by Sanger sequencing and had been reported before.Conclusions LGMD is clinically and genetically heterogeneous,and targeted NGS is powerful in defining the causal mutation of LGMD and helpful in investigating the exact genotype/phenotype analysis.

AIM To study whether inhibition of semicarbazide (Sem)-sensitive amine oxidases (SSAO) attenuates myocardial ischemia-reperfusion (I-R) injury. METHODS Male Sprague-Dawley rats were randomly divided into 4 groups. Each group consisted of 10 rats. Sham group: the ligature was placed under the left coronary artery (LCA), but not ligated. Sham+Sem group: Sem (30 mg·kg-1, ip) was given 10 min prior to the experiment, the LCA ligature was not ligated. I-R group: the LCA was occluded for 30 min and reperfused for 180 min. I-R+Sem group: Sem (30 mg·kg-1, ip) was given 10 min prior to the experiment, and then 30 min ischemia followed by 180 min reperfusion. Myocardial infarct size was determined by using nitroblue tetrazolium staining. Plasma creatine kinase (CK) and myeloperoxidase (MPO) activities, and malondialdehyde (MDA) and hydroxyl radicals levels were determined by spectrophotometer. Plasma SSAO activity was determined by high performance liquid chromatography. HE staining was used for histopathological evaluation. RESULTS There were no significant differences on each index between sham and sham+Sem groups. Plasma MPO and SSAO activities, and MDA and hydroxyl radials levels significantly increased in I-R group, compared with sham group. Myocardial infarct size was remarkably smaller in I-R+Sem group (27.7±3.7)%, compared with I-R group (43.2±3.1)%. Plasma MDA level, MPO activity and hydroxyl radical level were lower in I-R+Sem group than those in I-R group, from (27.5±9.3) μmol·min-1·L-1,(2.6±0.4)mmol·L-1 and (628±50)mmol·min-1·L-1 down to (14.2±5.6)μmol·min-1·L-1,(1.7±0.5)mmol·L-1 and (503±88)mmol·min-1·L-1, respectively. Histological results showed that inhibition of SSAO activity significantly attenuated leukocyte infiltration. CONCLUSIONPlasma SSAO activity is increased in myocardial I-R injury and inhibition of SSAO can attenuate myocardial I-R injury.