Objective To detect and determine the specificity of platelet-reactive antibodies in patients who were refractory to platelet transfusions.Methods Serum samples from 48 patients who were refractory to platelet transfusions were screened with MACE for platelet-reactive antibodies.Specificity of platelet alloantibodies was determined with PAK12 and MAIPA.Results Platelet-reactive antibodies were detected in the serum of 50% PTR patients(24/48).The incidence of HLA antibodies was 39.6%(19/48),accounting for 79.2% of serum with platelete alloantibodies.The HPA alloantibodies were found in 29.2%(14/48)serum,of which,64.3%(9/14)occurred together with anti-HLA.The following platelet-specific antibodies were identified:anti-HPA-3a(n=2),anti-HPA-5b(n=1),anti-HPA-5a(n=1),anti-HPA-2b(n=1),anti-HPA-4b(n=1).Of the 14 serum with HPA antibodies,78.6%(11/14)contained panreactive anibodies against platelet glycoprotein(GP)Ⅱb/Ⅲa,GPⅠa/Ⅱa,and/or GPⅠb/Ⅸ.Platelet-reactive antibodies were detected more in female(16/29)than in male(8/19)with a frequency of 55.2%,42.1%,respectively,but there was no statistical significant difference.Conclusion The platelet-specific antibody in PTR patients are not as rare as previous thought although alloantibodies are predominantly anti-HLA.Antibody specificities in Chinese PTR patients are different from those observed in Caucasians,in whom anti-HPA-5b and-1b are the most prevalent specificity.The most prevalent platelet-specific antibodies are anti-HPA-3 and anti-HPA-5 while anti-HPA-4b and anti-HPA-2b are also detected.

Objective:To explore a new way of nursing teaching round-making compatible with the nursing students at junior college,and to establish and improve the standards for its assessment.Methods:We performed a new mode of nursing teaching ward round jointly organized by nursing teachers of the school and clinical supervisors of the hospital among student nurses of Grade 2006 on four different subjects respectively,by going through the following four steps: establishing the subject,working out the plan,making the ward round and assessing the quality.Results:The average score on the quality assessment of the four subjects by teachers and students was 93.78.Conclusion:The new mode of nursing teaching ward round jointly organized by the school and hospital can make the best of nursing faculty resources,give full play to nursing students'potential and optimize this activity and facilitate the teaching of clinical nursing.

Objective: To explore an integral training mode for student nurses in large modern general hospitals.Methods: Integral nursing training was conducted in our hospital among 610 student nurses from universities and postsecondary specialized colleges.Results: All the student nurses considered the integral training to be helpful for the promotion of their professional nursing competence.Conclusion: The professional competence-oriented integral nursing training can optimize the teaching quality of clinical nursing and help enhance the professional competence and comprehensive qualities of student nurses.

Drug Resistance, Neoplasm ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Humans ; Ovarian Neoplasms ; drug therapy ; radiotherapy ; Radiation Tolerance

Objective To investigate the protective effect of pinacidil on the intestinal mucosa of scalded rats and the mechanism. Methods A total of 24 healthy SD rats were randomly divided into normal control group, burn group, and pinacidil treated group. Rats in burn group and pinacidil treated group were inflicted with 30% TBSA Ⅲ degree burn and resuscitated intraperitoneally with Ringer's solution immediately after burn. Pinacidil was injected intraperitoneally into rats in pinacidil treated group at the dose of 2 mg/kg. Mitochondrial respiratory function intestinal mucosa and levels of reactive oxygen species (ROS), maleic dialdehyde (MDA), and superoxide dismutase (SOD) in plasma were determined at 6 h after burn. Results Mitochondrial respiratory function control rate (RCR), ST3, and SOD levels in pinacidil treated group were evidently higher than those in the burn group. However, ST4, MDA, and ROS levels in pinacidil treated group were significantly lower than those in the burn group, but ST4 was not significantly different from that in the normal control. Conclusion Pinacidil can attenuate the damage of intestinal mucosa in scalded rats.

Bronchial asthma is the most common chronic diseases in children.Asthma can not be fully explained by imbalance of Thl/Th2.With the research progress of CD4+ CD25+ Treg cell,it has been found that CD4+ CD25+ Treg cell related factors such as forkhead/winged helix transcription factor,heine oxygenase-1,transforming growth factor-?,cytotoxic T lymphocyte-associated antigen-4 are closely linked to asthmatic mechanisms.

Objective To evaluate the prognostic value of white blood cell and hemoglobin in patients with acute aortic dissection(AAD).Methods White blood cell.hemoglobin and fibrinogen were measured in 42 AAD patients.The mortality during hospitalization was observed and the short-term prognosis in AAD patients was as-seased.Results There was 13 death during hospitalization.with the mortality of 30.95%.The value of white blood cell was higher in death group than in survival group[(13.73±6.91)×109>/L vs.(9.43±4.97)×109>/L.P< 0.05).The value of hemoglobin was lower in death group of than in survival group[(118.54±22.38)g/L vs. (131.72±18.17) g/L,P<0.05].There were no differences in the value of fibrinogen between the groups [(3.15±1.15)g/L vs.(3.48±1.24)g/L,P>0.05).The mortality in the group of elevated white blood cell(≥10.0×109>/L)was higher than that in the group of normal white blood cell(10.0×109/L)(41.18%vs.24%.P<0.05).The mortality in the group of decreased hemoglobin(≤110g/L)was higher than that in the group of nor-mal hemoglobin(>110 g/L)(50.00%vs.27.78%,P<0.05).Conclusion The value of white blood cell and he-moglobin could help to assess the short-term prognosis of patients with AAD.

A strain was separeted from the Yueqing bay using pine pollen baiting.The vegetative thallus of the separated strain is oval and unincleate.It possesses a cell wall composed of many compact layers of closely pressed scales, which can be resolved where the cell wall is disrupted.The radiating branched extensions of the thallus, the ectoplasmic net, emerges from the sagenogenetosome.Asexual reproduction is by conversion of the vegetative thallus to many biflagellate zoospores, during which tetrads of cells are formed.It was identified with Schizochytrium sp.based on the features mentioned above.

AIMTo investigate the effect of morp hine on purine nucleotides catabolism and its possible mechanisms. METHO DSRats were administered with morphine by intraperitoneal injection with increasing dose to develop ad diction model. The determination of uric acid concentration in plasma and the ac tivities of xanthine oxidase (XO) in the plasma and the small intestine were per formed. RT-PCR was used to examine the expressing level of key enzyme of purine nucleotides catabolism,XO mRNA. ?-actin was used as control gene in RT-PCR s tudy. RESULTSThe concentration of uric acid in plasma was sign ificantly increased in morphine-pretreated rats compared with control(P

0.05).(4) Significant differences between CC and CIN Ⅰ for p16,CDH1,RASSF1A and TIMP3 genes(P