Objective To evaluate the feasibility of combined application of helicobacter pylori stool antigen(HpSA)-and traditional serology in epidemiologic study of helicobacter pylori(Hp) infection in children. Methods Hp serum IgG antibodies were examined among 480 healthy children. Heticobacter pylori stool antigen(HpSA) were measured among children who were seropositive. Hp status was defined as positive when stool antigen test was positive, Hp status was defined as negative when seronegative. A case - control study was conducted to identify risk factors of Hp infection in preschool children. Results Among 480 preschool children, 172 (35 8 %) were sero- positive, 64(13.3 %) with positive result of HpSA test were diagnosed as current Hp infection. Univariate factor analysis demonstrated that Hp infection clustered in families and Hp infection was often acquired in childhood by oral-oral and fecal - oral person to person transmission. Conclusions HpSA and serology screening are two nomnvasive tests, simple not costly, effective and accurate. The combination of these 2 noninvasive methods can be applied in epidemiologic study of Hp infection in childrenJAppl Clin Pediatr,2004,19(11):948-950

Acquaintance of the lymph node is very important to evaluate whether the tumor is malignant or benign and therefore to the treatment of the tumors. Lymphography is now considered the gold standard for this purpose. Many computer aided diagnose (CAD) technologies have been developed to help radiologists to diagnose the tumor by the lymphography cases. In this paper, a computer aided diagnose model is constructed by Semi-naive Bayes Classification. The experiments carried out in our laboratory validated the Semi-Naive Bayes Classification on lymphography case. The result of experiments showed that Semi-Naive Bayes Classification could classify lymphography case effectively.
Algorithms ; Bayes Theorem ; Diagnosis, Computer-Assisted ; Humans ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; diagnosis ; Lymphography ; Neoplasms ; diagnosis

Objective To investigate the influence factors of malnutrition risk in hospitalized children,in order to provide theoretical basis for early identifying hospitalized children at the risk of malnutrition and for guiding clinical nutritional intervention.Methods Hospitalized children in the Department of Pediatrics of our hospital from March 1st 2013 to April 30th 2014 were included and assessed using Screening Tool for the Assessment of Malnutrition in Pediatrics (STAMP).Questionnaire survey was conducted and clinical data was recorded.The children were divided into two groups according to STAMP scores upon admission,namely high malnutrition risk group and low malnutrition risk group.Comparing the differences of basic characteristics,laboratory examinations,and treatments between the two groups,associated factors of statistical significance were detected.With the associated factors identified in single factor analysis as independent variables and STAMP score-based group division as the dependent variable,multifactor unconditional Logistic regression analysis was performed to explore the independent risk factors influencing STAMP scores of hospitalized children.Results A total of 1 406 hospitalized children were included,of whom 738 were at high malnutrition risk,and the other 668 were at low malnutrition risk.Single factor analysis indicated that fever before admission (Z =-3.809,P =0.000),severity of condition (x2 =14.068,P =0.000),age (x2 =5.813,P =0.017),and length of fever before admission (t =2.793,P =0.005) were associated with high malnutrition risk of hospitalized children.Non-conditional Logistic regression suggested that severity of condition (OR =1.557,95％ CI:1.164-2.083,P =0.003),length of fever before admission (OR =1.039,95％ CI:1.011-1.068,P =0.006),and granulocyte count (OR =1.032,95％ CI:1.004-1.061,P =0.027) were risk factors of high malnutrition risk in hospitalized children,and age (OR =0.942,95％ CI:0.909-0.977,P =0.001) was protective factor.Conclusion Age,severity of condition,length of fever before admission,and granulocyte count can provide helpful information for early identification of hospitalized children at high malnutrition risk.

Objective To evaluate transsphincteric operation (Mason operation) for rectal tumors.Methods Retrospective study was used to analyze the experience of Mason operation for 150 patients with mid and lower rectal tumors between Aug 1990 to Dee 2008. Results There were villous adenoma in 75 cases,early rectal cancer in 48 and advanced rectal cancer in 9,submucosal carcinoid nodule in 23.Of the 126 rectal tumor patients,120 underwent partial rectectomy,6 underwent segmental rectectomy.Seventeen out of the 21 rectovaginal fistula or rectourethral fistula cases underwent successful one-stage repair.Six patients(4%)developed wound infection.Five patients(3.3%)were complicated with recto-cutaneous fistula.Two patients(4.3%) suffered from local recurrence in 46 followed up early staged rectal carcinoma with a five-year survival rate of 84.5%(39/46).On discharge from hospital no patient suffered from incontinence.Conclusion Mason operation is satisfactory with good exposure and simple access to the rectum,which Was suitable for those lesions that could be locally resected on mid and lower rectum.

Objective To investigate the difference of serum prealbumin in hospitalized children and its value in Screening Tool for the Assessment of Malnutrition in Pediatrics ( STAMP) in hospital-izedchildren.Methods 867hospitalizedchildrenwererecruitedfromMarch2013toApril2014in the First Affiliated Hospital of Fujian Medical University .All the patients were assessed using STAMP and collected venous blood sample for measuring serum prealbumin within 24 hours after admission.All the patients were surveyed for information regarding gender , age, dietary changes, etc.and their clini-cal data and laboratory results during hospitalization collected .The patients were divided into high mal-nutrition risk group ( HMRG) and low malnutrition risk group ( LMRG) according to STAMP scores upon admission. Results There were 463 children ( 53.4%) in HMRG, and 404 in LMRG (46.6%).Compared with the LMRG, the HMRG had significantly lower serum prealbumin [ (144.7 ± 50.6) mg/L vs.(173.6 ±71.3) mg/L, t=6.795, P=0.000].After controlling for age, course of disease, white blood cell count, albumin, glutamic-oxalacetic transaminase, C-reactive protein in covariance analysis, the HMRG still had significantly lower serum prealbumin than the LMRG [ estimate ( 95% CI): 139.8 ( 134.9 -144.8 ) mg/L vs.157.9 ( 151.9 -163.8 ) mg/L, F =20.433 , P=0.000 ) .Clinical cure rates in HMRG with low serum prealbumin , HMRG with normal serum pre-albumin, LMRG with low serum prealbumin, and LMRG with normal serum prealbumin were 62.9%(95/151), 80.5% (251/312), 77.1% (27/35), and 98.1% (362/369) (χ2 =112.80, P=0.000 ) , respectively; incidences of hospital acquired infection were 21.9% ( 33/151 ) , 8.7%( 27/312 ) , 22.9% ( 8/35 ) , and 1.9% ( 7/369 ) (χ2 =63.55 , P =0.000 ) , respectively. Conclusion High malnutrtion could be distinguished more accurately using the combination of the as-sessment of malnutrition screening tools and serum prealbumin measurement .

Objective: To investigate the mechanism of forsythin in inhibiting the growth, migration and invasion of human renal cancer cells (786-0). Methods Human renal cancer cells (786-0) were cultured in vitro, and different concentrations of forsythin were added. Cell viability was detected by MTT assay, cell apoptosis was detected by AO/EB assay, cell migration and invasion abilities were respectively investigated by wound healing and transwell migration assays. The expression levels of PI3K, p-PI3K, Akt, p-Akt, FOXO3a, p-FOXO3a, p21, p27, Fasl, Bim, MMP-2, and MMP-9 were detected by Western bloting. Results:In 786-0 cells, forsythin effectively inhibited the growth of renal cancer cells, promoted apoptosis, interfered with cell cycle, and upregulated expression levels of p21, p27, Fasl, and Bim, when compared with control group (P < 0.05, 0.01); Compared with the control group, different concentrations of forsythin can significantly inhibit the migration and invasion of renal cancer cells, and reduce the synthesis of MMP-2 and MMP-9 (P < 0.05, 0.01). Meanwhile, forsythin can significantly inhibit the phosphorylation of PI3K, Akt and FOXO3a in a concentration-dependent manner (P < 0.05, 0.01). Conclusion: Forsythin can regulate apoptosis and cell cycle, and inhibit the growth of renal cancer cells by down-regulating the PI3K/Akt signaling pathway. At the same time, forsythin can effectively inhibit the ability of renal cell migration and invasion through the PI3K/Akt pathway.

OBJECTIVE To investigate the pathogenic causes of community-acquired pneumonia(CAP) in adult patients in Tongling.METHODS A prospective study was performed on 260 consecutive adult patients with CAP in Tongling city during last three years.Bacteria culture of sputum and serological tests in paired serum samples were detected.RESULTS Of 260 patients with etiological evaluation,128(49.2%) patients had an identifiable etiology,63(24.2%) had positive outcome from sputum cultured,atypical pathogens were detected from 75(28.8%)patients.Pathogens identified in 128 patients were:Mycoplasma pneumoniae(35.4%),Chlamydia pneumoniae(17.7%) and Streptococcus pneumoniae(13.6%).6.5% All patients had mixed infection.The resistance rate of S.pneumoniae to penicillin and erythromycin was 5 and 50%,respectively.CONCLUSIONS Atypical pathogens have important role in CAP,of which M.pneumoniae is the most common pathogen.S.pneumoniae and K.pneumoniae are the commonly encountered bacteria for CAP in Tongling.

Objective To explore the mediation role of university graduates' core self-evaluation played between perfectionism and job-selecting anxiety.Methods 200 university graduates were investigated by Chinese frost multidimensional perfectionism questionnaires,core self-evaluation scale and self-rating anxiety scale with convenience sampling method.Results ①Job-selecting anxiety had no distinct differences in gender,residence and the aspects of only-child(P＞0.05),but in different subjects,it was different(science and technology(1.79±0.49),culture and history(3.21±0.50),t=-4.38,P＜0.01).② University graduates' positive perfectionism was positively correlated with core self-evaluation (r=0.31,P＜ 0.01),while negatively correlated with job-selecting anxiety (r=-0.35,P＜0.01).University graduates' negative perfectionism was negatively correlated with core self-evaluation(r =-0.35,P＜ 0.01),while positively correlated with job-selecting anxiety (r =0.42,P＜ 0.01).Simultaneously core self-evaluation was negatively correlated with job-selecting anxiety (r=-0.46,P＜ 0.01).③ Core self-evaluation played a part of the intermediary role between negative perfectionism and job-selecting anxiety.The mediating effect was 30.0％ of the total effect.Core self-evaluation played a part of the intermediary role between positive perfectionism and job-selecting anxiety.The mediating effect was 34.5％ of the total effect.Conclusion Perfectionism as a personality trait can calculate the level of job-selecting anxiety.Core self-evaluation plays a part of the intermediary role between perfectionism and job-selecting anxiety.It is helpful to the working tactics of university graduates.

Objective To study the antibacterial constituents of Senecio cannabifolius.Methods The antibacterial effect of all the extracts was tested in vitro with Staphylococcus aureus,Bacillus subtilis(Gram-positive bacilli),and Escherichia coli(Gram-negative bacillus).Chemical constituents were isolated by chromatography.Physicochemical characters and spectroscopic analyses were employed for their structural identification.Results Five compounds were isolated and their structures were elucidated by chemical and spectral methods as follows: methyl-5-hydroxy-2-pyridinecarboxylate(E-13),6-hydroxy-7,7a-dihydro-2(6H)-benzofuranone(E-14),2-(1,4-dihydroxycyclohexanyl)-acetic acid(E-16),3-hydroxycyclohexanecarboxylic acid(E-17),and 4-hydroxy benzaldehyde(B-14).Conclusion All of them are isolated from the plants of Senecio L.for the first time.

The voltage-gated potassium channels Kvl.5 is widely expressed in the plasma membranes of numerous tumor cells and it can contribute to a variety of cellular functions such as proliferation,ap optosis,and cell cycle.Several types of antineoplastic drugs can change the expression of Kv1.5 and then affect the biological processes.Kv1.5 is identified as a novel target for therapy in human cancer.The researches of Kv1.5 will contribute to gain a further understanding of the molecular mechanism of tumor and provide therapeutic opportunity for the prevention and treatment of cancer.