Objective:To detect the expressions of COX-2 protein and BCL-2 protein in transitional mucosa adjacent to rectal carcinoma,and determine whether the transitional mucosa was the cancer precursor event.Methods:Mucin histochemical method was employed to detect the distal mucosa 2 cm away from rectal tumor and the transitional mucosa was found in 54 cases of rectal carcinoma.Immunohistochemical method was used to investigate the expressions of BCL-2 and COX-2 protein in the specimen of rectal carcinoma mass,transitional mucosa and non-transitional mucosa,and 20 cases of normal rectal mucosa,and the points of the expressions of COX-2 protein and BCL-2 protein were counted.Results:35.19%(19/54)of distal mucosa were characterized as the transitional mucosa.The expressions of COX-2 and BCL-2 protein were detected in carcinoma mass and distal mucosa.Significant difference was observed in carcinoma mass and transitional mucosa(p<0.01).But there were no significant differences between the expressions of those in transitional mucosa and non-transitional mucosa(p>0.05),as well as transitional mucosa and normal mucosa(p>0.05).Conclusion:The performance of transitional mucosa was not the cancer precursor event,but the non-specificity reaction of carcinoma or inflammation.

Objective To observe the effect of epidural injection with different dose of butorphanol on the rats' neurological function.Methods A PE-530 catheter was inserted into the epidural space of all the SpragueDawley rats (male, weighting 180 ～210 g) at L1-2 level.After three days, a total of 32 rats without any motor dysfunction were randomly divided into 4 groups as follows saline(NS) group (group C, n= 8 )and butorphanol injection (B) group( B1∶ n=8;B2∶ n=8;B3∶ n=8).Rats in group C were epidurally injected NS 30 μl each ,and rats in group B1, B2 and B3 were respectively epidurally injected Butorphanol 60 μg/30μl, 120 μg/30 μl,240 μg/30 μl (all diluted with NS) ,and 1 time per day for5 days.The neurological function of rats was recorded before injection (T0) and 6h after injection on day 1 ～4(T1 ～T 4) and 6h,24h and 72h after injection on day 5 (T5 ～T7) by BBB (BASSO,BEATTIE and BRESNAHAN ) Score and the inclined plane test .Results Compared with group C ,the BBB score and the inclined plane test of group B1 showed no significant difference throughout the experimental period(P＞ 0.05 ).There was also no significant difference at T0 ～ T3 of group B2 and group B3 compared with group C (P ＞ 0.05 ), while at T4, the BBB score ( ( 18.50 ± 2.00 ) points, ( 16.38 ± 2.33 ) points) and the inclined plane test( (58.75 ± 5.17 )°, (59.38 ± 3.20) ° ) of the two groups were both obviously decreased when compared with group C( (21.00 ±0.00) points, (65.00 ±3.78)°, P＜0.05) ,and the same significant differences appeared at T5,T6 and T7 (P ＜ 0.05 ).Conclusion Repeated epidural injection of butorphanol 60 μg have no effect on neurological function of rats,while repeated epidural injection of butorphanol 120 μg and 240 μg could impaire the neurological function.

Objective To investigate the relationship between the gene polymorphism of the inducible costimulatory molecules (ICOS),CD_(28),CD_(24) and susceptibility to multiple sclerosis(MS). Methods 83 patients with MS and 110 controls selected from healthy individuals and hospital staff in Chinese Han people with non-autoimmune diseases were studied by detecting genotype of the 3 genes using PCR-RFLP method. Results The frequency of ICOS-2394 TT genotypes was significantly higher in MS patients than in controls (MS 33.7%vs controls 10.9%, P

Objective To know the rural doctors' knowledge,belief and practice on iodine deficiency disorders(IDD),as well as their capability on health education communication in Ye County,Henan Province.Methods Semi-structured interviews were conducted among 50 rural doctors in Ye County,Henan Province.Topics covered include:sales of salt purchases,daily supervision and monitoring activities of salt,the health effects of salt,the situation of rural doctors trained,the situation of rural doctors participated in health education and related recommendations.Results ①Twenty-nine of the rural doctors knew exactly the advantage of salt iodization.②Thirty-nine of the rural doctors had taken part in the half quantitative detection of salt once or twice a year and 15 of them knew the supervision activities of salt industry department.③Forty-nine of the rural doctors believed salt iodization was beneficial to health and 48 of them were willing to participate in health education on IDD.④The major ways of their acquiring health knowledge were promotional materials [54.0％ (27/50)],TV [50.0％ (25/50)],and lecture/training [24.0％ (12/50)].⑤Thirty-seven of the rural doctors had taken part in health education on IDD.They were used to use booklets [68.0％ (34/50)],promotional slogan [36.0％ (18/50)],broadcast [24.0％ (12/50)],and doctor consultation [24.0％ (12/50)].⑥Twenty-eight of the rural doctors thought the current health education on IDD was better.They hoped to reduce the price of salt,strengthen supervision,receive more training and health education on IDD.Conclusions The rural doctors remain relatively lack of knowledge on IDD.Rural doctors have the initiative and responsibility to conduct health education on IDD,but their pattern is still a one-way propagation.

Thirty-four cases out of 830 children with diarrhea were confirmed to be positive of fecal cryptosporidial oocysts with IFA technique and monoclonal antibodies in a period of 2 years. These 34 cases could be diagnosed as cryptosporidial enteritis. Their age ranged from 4 months to 7. 6 years with an average of 2. 5?1. 7 years. The stool was frothy and watery in appearance. High incidence occurred form June to September. Determination of PHA skin test, LBT and serum Ig was performed on 27 patients before, during and after the treatment of Astragalus membranaceus Bunge and Sophora flavescens Ait ( 2 traditional Chinese herb medicines). It was found that diarrhea lasted 10. 9?7. 1 days and oocyst shedding lasted 21. 5? 12. 3 days. Before treatment) PHA skin test showed an erythema of 7. 9?2. 4 mm in diameter, and LBT value was 48. 7%?10. 1% and they returned to normal after treatment.It is suggested that hot safrain-methylene blue stain and modified acid-fast stain of the stool be performed in those children with diarrhea of unknown etiology as a routine screening for cryptosporidial oocysts. The 2 Chinese herb medicines may have a repelling effect on the parasites and be. helpful to the cell immunity of the patients. Asymptomatic carriers of cry-tosporidial oocysts may be the source of infection.

Objective: To examine the causal relationship between food intake and atherosclerosis using two-sample Mendelian randomization (MR) approach, so as to provide the reference for the prevention of atherosclerosis. Methods: Data of 16 types of food (grains, vegetables, fruits, meats, etc.) and 4 types of atherosclerosis (coronary atherosclerosis, cerebral atherosclerosis, peripheral atherosclerosis and other atherosclerosis) was collected through IEU OpenGWAS database, with food data comprising approximately 500 000 subjects and 9 851 867 SNPs and atherosclerosis data comprising approximately 200 000 subjects and 16 380 447 SNPs. The causal relationship was analyzed using inverse-variance weighted (IVW) method with food as the exposure variable and atherosclerosis as the outcome variable. Sensitivity analysis was performed using funnel plots and leave-one-out. Results: Dried fruit intake was associated with decreased risks of peripheral atherosclerosis (OR=0.195, 95%CI: 0.082-0.466) and other atherosclerosis (OR=0.208, 95%CI: 0.095-0.452), and cheese intake was associated with decreased risk of peripheral atherosclerosis (OR=0.575, 95%CI: 0.380-0.870). Coffee intake was associated with increased risk of coronary atherosclerosis (OR=1.645, 95%CI: 1.099-2.462), and alcohol intake was associated with increased risk of other atherosclerosis (OR=1.269, 95%CI: 1.032-1.561). There was no statistically significant association between 16 types of food and cerebral atherosclerosis. No horizontal pleiotropy was found, no single SNP had significant impact on the overall estimated value, and the funnel plots did not show significant bias. Conclusion Dried fruit and cheese intake are protective factors for atherosclerosis, while coffee and alcohol intake are risk factors for atherosclerosis.

Purpose To investigate the effects ofβ-1apachone on inhibition of pro1iferation and migration and induction of apoptosis in gastric cancer ce11s in vitro. Methods The ce11 viabi1ity was detected using MTT and co1ony formation assay,the migration abi1ity was determined using scratch assay method,and the apoptosis was examined using f1ow cytometry. Meanwhi1e,the expression of biomarkers of pro1iferation,EMT markers andapoptosiswere detected using Western b1ot ana1ysis. Results β-1apachone cou1d significant1y inhibit the pro1iferation of SGC-7901 and AGS gastric cancer ce11s( P<0. 05),and down-regu1ate the expression 1eve1s of Skp2 and DEK pro-teins. β-1apachonecou1d a1so inhibited the invasion and moti1ity of gastric cancer ce11s via down-regu1ating the expression 1eve1s of MMP-2/9 and Ezrin proteins and up-regu1ating the epithe1ia1 markers. In addition,β-1apachone enhanced the apoptosis of gastric canc-er ce11s,down-regu1ation of BCL-2/Bax ratio and up-regu1ation of activated Caspase-3/8/9. Conclusions β-1apachone can effective1y inhibit the pro1iferation and induce the apoptosis of gastric cancer ce11s,and inhibit the migration of gastric cancer ce11s via MMPs and EMT pathways.

Objective To analyze the influence of Absidia corymbifera on cell activity of human umbilical vein endothelial cells (HUVEC) as well as the related mechanism. Methods Time course analy sis of the influence of A. corymbifera on cell viability of HUVEC was determined by cell counting after Trypan blue staining. Apoptosis of HUVEC induced by A. corymbifera was observed under fluorescence microscope after treatment with apoptosis detection kit. Time course analysis of HUVEC apoptosis induced by A. corymbifera was detected by flow cytometry quantitatively. Effect of caspase-3 inhibitor on A. corymbifera associated apoptosis was also evaluated at the same time. Activation of caspase-3 inside HUVEC was detected by Western blot. Results A. corymbifera inhibited cell viability of HUVEC in a time-dependent manner by Trypan blue staining. After 12 hours' co-culture, A. corymbifera began to show suppression on cell viability (P =0. 001 ). Fluorescence microscope observation revealed A. corymbifera induced apoptosis of HUVEC instead of necrosis. Flow cytometry analysis showed A. corymbifera induced apoptosis of HUVEC in a time-dependent manner. A. corymbifera began to show obvious effect on apoptosis after 12 h co-culture (P =0.0036). Moreover, A. corymbifera-associated apoptosis was almost abrogated completely by caspase-3 inhibitor. Western blot analysis demonstrated that A. corymbifera triggered the activation of caspase-3 inside HUVEC in a timedependent fashion. Conclusion A. corymbifera induces apoptosis of HUVEC in vitro. Such apoptotic signal is transmitted through caspase cascade reaction.

ObjectiveThe trial was to examining the effectiveness and cost effects of family intervention for rehabilitating chronic outpatients with schizophrenia in the country.Methods90 subjects were randomly assigned to the family intervention group and the control group. Both groups received the same treatments, but the family intervention courses mainly containing mental health education were given to the family intervention group for one year. During the time, all subjects were evaluated with standard rating scales and self made criteria. ResultsThe family intervention group demonstrated clinical results significantly superior to those of the control group on overall improvement according to the scores on the SDSS, the SAPS, the SANS and the MRSS. Substantially, the direct and indirect average cost in the family intervention group was significantly lower than those of the control group. Conclusions Family intervention is effective not only in making the schizophrenics recover from illness but also in both increasing their social functions and reducing their medical cost.